Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

International Journal of Biosciences and Biotechnology

jbb
Website

Published by Universitas Udayana

ISSN : 23033371 EISSN : 26559994 DOI : -

Core Subject : Science, Social,

Biochemistry, Genetics & Molecular Biology Environmental Science

International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences

Arjuna Subject : -

Articles 160 Documents

1 2 3 4 5

ELECTRIC STUNNING OF CATTLE FOR SLAUGHTERING AND SECURING THE BEEF FROM MICROORGANISM Wayan Sayang Yupardi; I Made Nuryasa; Ni Luh Putu Sriyani; Eny Puspani; I Gede Suranjaya
International Journal of Biosciences and Biotechnology Vol 3 No 2 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.647 KB)

Stunning electricity current of 1.0 A, 200 – 300 V were caused the destruction of cattle’s centralnerve. This is very important to drain blood as much as possible from the whole body. Thiscauses its beef not become a media of unexpected microorganism to develop theirself.In themodern age right now, electricity is needed much for living on the earth or outer space. In otherwords, live is much depend on it. Indonesia is a tropical and agricultural country with highhumidity. These are good media for microorganism to develop theirself i.e. in aerobic conditionsuch as Pseudomonas, Achromobacter (induce rancidity in), Strepcococcus, Leuconostoc,Bacillus (causes mucus on beef surface), Lactobaccillus (causes the changes of beef colour fromred to greenish), Photobacterium (causes phosphorescent). anaerobic codition Achromobacterand Proteus cause sour taste. The beef or other food stuff become spoiled fast if they are notpreserved in low temperatureIt is necessary to control the room temperature. The objectives ofthis study were to know and open insight about physiological works of electricity in order toprovide healthy beef as a source of animal protein to form a bright nation. The beef can bepreserved more than 30 days without decreasing its hygiene level in the temperature of 5 – 7oC.At these low temperatures the microorganism mentioned above can delayed their growth anddevelopment.

SUGAR PRODUCTION BY DIGESTING OF OIL PALM EMPTY FRUIT BUNCH USING EXTRACELLULAR ENZYMES FROM Aspergillus niger AND Trichoderma reesei FOR ETHANOL PRODUCTION Kahar Muzakhar; Sutoyo S; Siswoyo S
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.426 KB)

Extracellular enzymes which obtained from 4 days cultivation Aspergillus niger and Trichoderma reesei onsolid state fermentation of oil palm empty fruit bunch (OPEFB) were used for lignocellulosic-??rich OPEFBdigestion. The enzymes were concentrated using 70% saturated ammonium sulphate, dialysed against20mM acetate buffer at pH 5 and adjusted one tenth (v/v) from the initial volume with the same buffer.The concentrated enzymes were then used in hydrolysation of powdered OPEFB. Amount of 10.65 mg/ mland 11.47 mg/ml sugars were produced when each concetrated enzyme A. niger and T. reesei mixedwith2%OPEFB. These hydrolysation were done on 100 ml total volume, incubated at 37oC with 100 rpmshaken for 36 hours. Further, both hydrolyzates results were sterilised and fermented anaerobically usingSaccharomycess cerevisiae at concentration 0.5mg/ml cells and incubated in 30oC for 24 hours. Colorimetricanalysis using QuantiChrom Kit DIET-??500 at OD 580nm gave results the alcohol production were 0.86%and 0.92% which were similar with Gas Chromatograph analysis that of 0.83% and 0.93%, respectively.

PHENOTYPIC, GENOTYPIC CHARACTERS AND NUTRITIONAL VALUE OF SEEDLESS WANI (Mangivera caesia Jack. var. Ngumpen Bali) (A Review) I Nyoman Rai; Cokorda Gede Alit Semarajaya; Gede Wijana; I Wayan Wiraatmaja; Ngurah Gede Astawa; Ni Komang Alit Astiari
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.899 KB)

The diversity of Mangivera caesia Jack (Balinese name: wani) in Bali was quite high. Based on the morphological characters of the fruit, 22 cultivars had been explored in the previous research (Rai et al., 2008). One of the most superior cultivar among those and very potential to be commercially developed was seedless wani (M. caesia Jack. var. Ngumpen Bali). The cultivar had specific properties that were not possessed by the others. Ninety (90) % of the total fruits produced were seedless, while the remaining (10%) has small seed. Beside that, this seedless cultivar had thick flesh, very attractive skin color (glossy yellowish green), uniformity on the size and shape of fruits, a distinctive aroma, sweet, tasty, and highly nutritious. The results of RAPD analysis of 10 wani cultivars grown in Bali showed that this seedless cultivar (Ngumpen) was grouped in to different cluster, a part from others. In comparison with 4 seeded cultivars of wani, Ngumpen cultivar had a similar nutrient content, however, it had greater fiber and a greater percentage of edible part. We concluded that the Ngumpen cultivar was a specific and unique germplasm so that should be preserved and protected.

THE EFFECTS OF DIETARY INCLUSION OF DETOXIFIED Leucaena leucocephala LEAF MEAL ON THYROIDAL ACTIVITY OF RATS DURING GESTATION-LACTATION PERIOD Ngurah Intan Wiratmini; Inna Narayani; Ni Luh Eka Setiasih
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Leucaena leucocephala provides the highest amount of proteins compared to other greens. However, its use is restricted due to the content of mimosine, an antinutrition non-protein amino acid known to be toxic to animals. This study aimed to observe the effect of administration of more than 10% of processed Leucaena leucocephala leaf meal to the level of T3, T4 and the thyroid histopathology. In this study, Leucaena leucocephala leaves were soaked in distilled water for 12 hours. The processed of Leucaena leucocephala leaf meal was made into pellets containing 0%, 7.5%, 15%, and 22.5% leaf meal of total feeds respectively, and was fed to the rats during pregnancy and lactation. The level of triiodothyronine (T3) and thyroxine (T4), and the thyroid histological features were the parameters observed. The collected data were statistically analyzed by SPSS 20.0 for Windows using One-way ANOVA followed by Duncan Multiple Range Test to observe any significant difference among the 4 treatment groups (?=5%). Meanwhile, the presence of hyperplastic cells and follicle lumens filled with vesicles and colloids was descriptively analyzed by means of available literatures. The analysis of T3 and T4 level revealed that there was no any significant difference between the control and treatment groups. The epithelial cells of thyroid follicles in the treatment groups of P1, P2 and P3 showed hyperplasia and were detached from their respective basal membranes.

THE USE OF ADVANCED GENOMIC PLATFORMS TO ACCELERATE BREEDING PROGRAMS OF THE INDONESIAN AGENCY FOR AGRICULTURAL RESEARCH AND DEVELOPMENT (A Review) I Made Tasma
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (711.282 KB)

The use of advanced genomic platforms such as next generation sequencing (NGS) and highthroughput SNP (HT-SNP) genotyping platform facilitates the use of PGR collection in amore comprehensive manner for a more efficient breeding program. Indonesia is recognizedas the second richest mega-biodiversity in the world. This includes the plant and animal GRof agricultural importance. Few excellent example PGR of Indonesian origin included rice,banana, and sweet potato. The available GR diversity richness must be manipulated forhuman kinds (e.g. breeding purposes) to develop superior crop and animal for food, feed,ornament, and industry. A genomic-based breeding program facilitates the manipulation ofthe wealth GR collection in a more comprehensive, effective, and efficient manner. Thispresentation describes the current status of NGS-based genome sequencing project as well asthe application of HT-SNP array technology in genomic and breeding projects of theIndonesian Agency for Agricultural Research and Development (IAARD). The main cropsand animal under study included soybean, maize, rice, cacao, chili pepper, potato, physic nut,oil palm, and cattle. The sequencing project was done using NGS HiSeq2000 and the SNPchip genotyping study was done using the Illumina iScan. Genome Browser (GB) of thegenomic data was developed containing millions of genomic variations (SNP, Indels, andSSR). The GB is an excellent breeding resource to support breeding program of the crop andanimal under study. The genome browser also contains phenotypic and SNP genotypic dataof specific targeted populations. The SNPs discovered in this study are marker resources forHD SNP chip development. The SNP chips are useful for HT-SNP genotyping projects, GRcharacterization, and gene tagging to identify superior genes and QTLs for characters ofinterest to accelerate national breeding program of the national priority crops and animalunder study.

ISOLATION, CHARACTERIZATION AND INOCULUM FORMULATION OF NODULE FORMING BACTERIA OF KUDZU (Pueraria phaseoloides (Roxb.)Benth.) FOR COASTAL SANDY LAND CONSERVATION Nike Triwahyuningsih; Tati Budi Kusmiyarti
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Kudzu plantation (Pueraria phaseoloides) as legume cover crop is one of alternatives in coastal sandy land conservation. The crops are known to associate with a root nodule-forming bacteria (Rhizobium sp.) which give some benefits to nutrient cycling i.e. : atmospheric N2 fixing and play role as soil conditioner; soil Nitrogen enrichment; nutrient cycling; and increasing other nutrients availability. A research to study the isolation dan bacterial inoculum multiplication from wild kudzu root nodules, compatible isolates screening and selected isolates multiplication, and examining the form, amounts and most proper inoculum application method was conducted in Greenhouse and Laboratory of Microbiology in Yogyakarta province.The research were held in four phases : (1) isolation, purification and characterization of isolates; (2) reinoculation dan compatibility testing of isolates to kudzu seeds; (3) inoculum multiplication; and (4) examination of the form, amounts and most proper inoculum application method. Physical and biochemical properties of the isolates were observed during the isolation phase. Infection and nodulation activity were observed during the reinoculation phase. Indirectly counting of the microbial numbers to obtain the cell numbers was conducted during the inoculum multiplication. While infection and nodulation activity and plant growth were observed during the inoculum testing phase.Isolates purification on Yeast Mannitol Agar + congo-red media gave 5 different isolates named RP-Etp1, RP-Etp2, RP-Etp3, RP-Etp4, RP-Etp5. The RP-Etp4 isolate had the highest compatibility to the kudzu seeds (number of effective nodules >100 per plant), followed by RP-Etp5 (medium compatibility, number of effective nodules 50–100 per plant), RP-Etp1 and RP-Etp3 (low compatibility, number of effective nodules < 10). Isolate RP-Etp2 was incompatible to the kudzu.Optimum cell numbers was reached in 48 hours incubation time. Application of broth/liquid inoculum of Rhizobium sp. has advantages over solid inoculum (in peat) as it gives the highest number of nodules, and the optimum dosage was 2 – 4 ml per plant. The highest infection-nodulation activity and plant growth were reached in 4 ml inoculum per plant (direct application) or 2 ml inoculum per plant (weekly applied in two weeks).

Guide for Authors Guide for Authors
International Journal of Biosciences and Biotechnology Vol 3 No 2 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.743 KB)

Guide for Authors

EXPLORING STRIP INTERCROPPING POTENTIALS OF MAIZE-PULSE CROPS TO FIGHT CLIMATE VARIABILITY IMPACTS IN DRYLAND AREAS I Komang Damar Jaya; Sudirman Sudirman; Rosmilawati Rosmilawati
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Recent climate variability affects maize production in dryland areas. This study aimed to explore potentials of strip intercropping of maize-pulse crops in improving productivity of dryland areas. The study was conducted in dryland area of Gumantar village, North Lombok (8.253654 S, 116.285695 E). Soil in that area was categorized as poor soil with the following properties: 0.46% organic matter, 0.05% N total (Kejdhal), available P 11.25 ppm (Olsen) and exchangeable K 0.77 me%, pH 7.0 and field capacity 29% (%/V). Rainfall data were collected during the growing seasons of 2016/2016 and 2016/2017. A field experiment of maize-pulse crops strip intercropping was conducted during a dry season of 2016. The component crops in the strip intercropping were maize NK212, maize NK7328, mungbean Vima-1 and groundnut Hypoma-1. All component crops were grown as monocropping and strip intercropping of maize-pulse crops in 8.4 x 5.0m plot size for each treatment. To measure productivity of the strip intercropping, relative yield total (RYT) and benefit to cost ratio (B/C) were calculated. They were great variations in rainfall in the last two years. From the experiment, data showed that all the strip intercropping treatments have RYT and B/C values >1 meaning that strip intercropping of maize-pulse crops is more productive than monocropping and is feasible to be practiced in dryland areas. With the short growing period and their drought tolerant nature of the pulse crops, especially mungbean, the strip intercropping can be used to fight climate variability impacts in dryland areas.

THE POPULATION SUCCESSION PATTERNS OF CABBAGE MAIN PEST Plutella xylostella L. AND Crocidolomia pavonana Fab AT CABBAGE PLANTATION Ketut Ayu Yuliadhi; I Wayan Supartha; I Nyoman Wijaya; Pudjianto Pudjianto
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.173 KB)

This study was aimed to investigate the population dynamics of the cabbage insect pests, Plutella xylostella and Crocidolomia pavonana under natural conditions. The population succession patterns of both pests were determined by observing the abundance of their population on a regular basis from the beginning to the end of the planting season. Understanding the population succession patterns of P. xylostella and C. pavonana will give an idea the shifting time by both pests on attacking the crops, therefore on deciding the time of controlling. The population succession patterns occur in 8 weeks of planting season. P. xylostella dominated the cabbage plants when they were 1-5 weeks after planting (WAP), but when the cabbage plants were more than 5 weeks old they were dominated by C pavonana.

A PERSPECTIVE ON GLOBAL FOOD SECURITY-WHERE FROM HERE? Acram Taji; Tanya Tapingkae
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2012)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.874 KB)

A PERSPECTIVE ON GLOBAL FOOD SECURITY-WHERE FROM HERE?

Page 2 of 16 | Total Record : 160

1 2 3 4 5

Filter by Year

2012 2024

Filter By Issues

All Issue Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY Vol 1 No 1 (2023): Special Issue February (Online First) Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY Vol 9 No 2 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY Vol 8 No 2 (2021): International Journal of Bioscience and Biotechnology Vol 8 No 1 (2020): Vol. 8 No. 1, September 2020 Vol 7 No 2 (2020) Vol 7 No 1 (2019) Vol 6 No 2 (2019) Vol 6 No 1 (2018) Vol 5 No 2 (2018) Vol 5 No 1 (2017) Vol 4 No 2 (2017) Vol 4 No 1 (2016) Vol 3 No 2 (2016) Vol 3 No 1 (2015) Vol 2 No 2 (2015) Vol 2 No 1 (2014) Vol 1 No 2 (2013) Vol 1 No 1 (2012) More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota denpasar,

Bali

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kota denpasar, Bali INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota denpasar,

Bali

INDONESIA