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Pro-Life
Published by Universitas Kristen Indonesia
ISSN : 23020903     EISSN : 25797557     DOI : 10.33541
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology
Ilmu Biologi merupakan salah satu ilmu yang terus bertumbuh dan menjadi bagian yang penting dalam kehidupan. Ilmu Biologi mencakup kajian teori, penelitian hingga pendidikan Biologi menjadi cakupan ilmiah yang terus diteliti dan dikaji hingga memperoleh manfaat bagi kehidupan. Program studi pendidikan Biologi FKIP-UKI ingin memberikan wadah bagi para peneliti baik di dunia Ilmu Biologi maupun Pendidikan Biologi untuk mempublikasikan hasil kajiannya. Jurnal ini terbentuk pada tahun 2014 dan menjadi referensi bagi para mahasiswa dan peneliti lain dalam melakukan penelitian. Bidang spesifik yang dicakup dalam Jurnal adalah Pendidikan dan Ilmu Biologi. Cakupan/ fokus masalah yang dicakup dalam Jurnal antara lain Pendidikan, Metode Ajar, Tumbuhan, Hewan dan Lingkungan. Jurnal terbit 3 kali dalam satu tahun, yaitu pada Bulan November, Maret dan Juli.
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Articles 10 Documents
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Search results for , issue "Vol. 10 No. 1 (2023): Maret" : 10 Documents clear
Potensi Minyak Sereh Wangi (Cymbopogon nardus) sebagai Antibakteri terhadap Propionibacterium acnes dan Staphylococcus aureus pada Sediaan Gel Antijerawat Kania Apenta Olisvelos; Dwi Aditiyarini; Aniek Prasetyaningsih
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Acne can be caused by the bacteria Propionibacterium acnes and Staphylococcus aureus. One way to treat acne is to use antibiotics. However, this can create resistance. In this study, citronella oil will be formulated into an anti-acne gel preparation with various concentrations of 10%, 15% and 20%. The study began with distillation of citronella oil and analysis and identification of compounds. Then the gel was made by mixing citronella oil in the HPMC gel base and then the gel preparation was tested for antibacterial activity by the disc diffusion method and physical preparation tests included organoleptic tests, pH measurements, viscosity tests, homogeneity tests, dispersion tests, gel stability tests. Based on the results of compound analysis using GC-MS, citronella oil contains geraniol (55.05%). Of the three formulations, the preparation that has good gel physical properties based on its physical properties is F2 with 15% citronella oil content. In the antibacterial test, the results showed that citronella oil gel had the ability to inhibit Propionibacterium acnes and Staphylococcus aureus with the most optimal formulation being F2 with an average inhibition of 1.5 cm.
Uji Aktivitas Antikoagulan pada Sel Darah Manusia dari Ekstrak Bawang Bombai (Allium cepa L.) Fany Febriani; Junius Hardy; Riskianto
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Thromboembolic disorders can cause various life-threatening cardiovascular diseases. Anticoagulants are the most common treatment for thromboembolic disorders. Organosulfur compounds in onions, such as allicin and flavonoid compounds, have anticoagulant properties. The purpose of this study is to test the anticoagulant activity of onions extracted by maceration method using 96% ethanol as a solvent. The anticoagulant test was performed on samples of blood groups A, B, AB, and O using the Lee-White method. Six tubes were filled with 1 cc of each blood sample with treatments including control (without treatment), negative control (96% ethanol), positive control (EDTA), and variation of extract concentrations 5; 7.5; 10%. The results of 96% ethanol extract of Onion (Allium cepa Linnaeus) using the maceration method contains secondary metabolites including flavonoids, saponins, and phenols. The onion extract had a significant mean difference (p < 0.05) with the EDTA positive control group and did not produce a significant mean difference (p > 0.05) with the untreated group, so the onion extract with a concentration of 5; 7.5; 10% extracted with 96% ethanol solvent using maceration method had no anticoagulant activity. Blood type A, B, AB, and O did not have a significant difference in the mean blood clotting time with a value of P = 0.504 (p > 0.05).
Inventarisasi Arthropoda pada Tanaman Tebu (Saccharum officinarum L.) Di Tiga Umur Tanaman yang Berbeda Zainal Abidin; Funky Dwy Ushardyna; Arief Lukman Hakim
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Sugarcane (Saccharum officinarum L.) is a national sugar-producing plant. One of the declines in sugar production is caused by pests and diseases and improper control. The study aims to make an inventory of arthropods in sugarcane at the age of 1,5 – 3 months, 4 – 10 months, and < 11 months in order to obtain information on these matters. The research was carried out in three plantation areas in Sumber Perkul, Gedangan, Malang and at the Basic Laboratory of the Universitas Islam Raden Rahmat Malang during March – May 2022. This study used the relative method (Sticky yellow traps, pitfall traps, light traps) and the absolute method (visual control). The results of this 14.932 individu, study obtained 68 families from the Arachnida and Insecta classes, with groups of predatory roles, parasitoids, herbivores and others. The Important Value Index of dominant natural enemies (Parasitoid) Dolichopodidae (45%), Detritivor arthropod (Drosophilidae) and Polinator arthropod (Noctuidae) (26%), Herbivora arthropod Delphacidae (15%), and natural enemies (Predator) Coccinelidae (14%). The conclusion is that from all the calculated indicators there is no group of arthropods that dominates, the diversity is moderate, and the distribution is evenly distributed. There is a change in composition, but in all indicators the calculation of the entire age of the sugarcane plant is still in the stable and balanced category.
Efektivitas Virtual Laboratory Dibandingkan Real Laboratory pada Perkuliahan Hybrid terhadap Kemampuan Berpikir Kritis Mahasiswa Dewi Nilam Tyas; Barokah Isdaryanti; Feylosofia Putri Agry
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Students have the opportunity to carry out Biology practicums through real laboratories in labs and virtual laboratories during post-pandemic hybrid lectures. In this regard, this study aims to compare the effectiveness of real laboratory and virtual laboratory and compare the effectiveness of both through hybrid lectures in improving the critical thinking skills of elementary school teacher education students at Universitas Negeri Semarang. This type of research is a quasi-experimental study with a control group pretest-post-test design. The population in this study were second-semester students taking science laboratory courses, with a sample of 94 students. The research will be carried out at the elementary school teacher education campus, Universitas Negeri Semarang, Central Java in April-June 2022. Biology practicum activities using real laboratories as opposed to using virtual laboratories show that virtual laboratories and real laboratories are effective in improving critical thinking skills. The N-gain score in the virtual laboratory group was 0.4494 (medium) and the N-gain score in the real laboratory group was 0.4867 (moderate). Even though the N-gain is in the moderate category, the score is higher in the real laboratory, so it is concluded that the real laboratory is more effective for increasing critical thinking skills through hybrid lectures.
Ulasan Aspek Etnobotani dan Fitokimia pada Tumbuhan Andaliman (Zanthoxylum acanthopodium DC.) oleh Suku Batak Di Sumatera Utara Bernadheta Rumondang Saragih; Reza Raihandhany
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Andaliman (Zanthoxylum acanthopodium DC.) is an essential plant for the Batak people who utilize its fruit and seeds as spices for cooking, due to the tart taste. Apart of being food needs, andaliman is often used as a traditional medicine and traditional ceremonies. This research was conducted using a literature study method on various scientific using some keywords on the google scholar site. Aspects which were collected including the information on botany, ecology, distribution, local names, ethnobotany as local food and traditional medicine, and phytochemicals. Andaliman fruits and seeds are used as ingredients for cook such as arsik, sangsang, sup trites, gulai biawak, tasa telu, and kidu-kidu. In traditional medicine, andaliman is used to cure various diseases such as improving blood circulation, asthma, rheumatism, and increasing appetite. Besides for food and traditional medicine, andaliman can be utilized in agroforestry schemes as a plant to support natural environment conservation. Extract of andaliman fruit and seed contain various secondary metabolite chemical compounds, including alkaloids, flavonoids, glycosides, saponins, tannins, and triterpenoids. In the end, information on the utilization of andaliman becomes a consideration for maintaining its existence.
Studi Keanekaragaman Invertebrata Di Kawasan Perairan Teluk Maumere Nusa Tenggara Timur Sitti Arafah; Hastuti Juniyati
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

The research objective was to determine the diversity of invertebrates in the waters of Maumere Bay, East Nusa Tenggara. This research was conducted in July-August using an exploratory descriptive approach. Sampling using purposive sampling technique. The results of the study found 150 species with descriptions: 26 species of Porifera phylum, 22 Coelenterata phylum, 67 Mollusca phylum, 5 Arthropoda phylum, and 30 Echinodermata phylum. Ecological parameters: Invertebrate density 2.05/m2-0.227/m2, relative density (RD) 0.017-0.001. The highest density value was from the type of Conus teselatus and the lowest density was from the type Aphrocallistes vastus. Frequency 0.017-0.001, relative frequency (RF) 0.015-0.001 with the highest frequency value of the Conus tesulatus type and the lowest frequency of the Aphrocallistes vastus type. The highest Importance Value Index for Conus tesulatus is 0.033 and the lowest for Aphrocallistes vastus is 0.002. The Shannon-Wiener species diversity index (H') value is 784,562 or the high richness category and the evenness value (E) is 77,856 which means that quantitatively the density value among community members is evenly distributed (E passes 1). The distribution pattern of all types of invertebrates is grouped with a Morisita Index (IM) value of 11.46-38.78 or an IM value > 1. The diversity of invertebrates in the study area is strongly influenced by environmental factors such as temperature, pH, salinity, and substrate which can reflect good conditions for the growth of invertebrates.
Pemanfaatan, Toksisitas dan Glikosida Jantung dari Digitalis purpurea L Marina Silalahi
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Digitalis purpurea (Schrophulariaceae) is used by the local Indonesian community as an ornamental plant, even though this plant has high toxicity and has the potential to treat heart problems. This study aims to explain the botany, bioactivity and toxicity of D. purpurea based on literature studies. Literature was obtained online, especially Google scholar using the keywords Digitalis purpurea, bioactivity D. purpurea, and glycoside heart. In Europe, D. purpurea is very popular to treat congestive heart failure and has been used since 200 years ago, but it is very toxic to humans. Digitalis poisoning causes anorexia, nausea, vomiting, and diarrhea, yellow or jaundice vision, appearance of blurred lines, decreased heart rate, and weight loss. D. purpurea's bioactivity includes overcoming heart failure, antioxidants, anti-cancer/tumor. Cardiac glycosides present in D. purpurea include cardenolides, digitoxin, gitoxin, purpureaside A, purpureaside B, acteoside and purpureaside C, which are related to their bioactivity. The toxicity and inotropic effects of cardiac glycosides result directly from inhibition of membrane-bound Na+K+_ATPase, an enzyme involved in the transport of Na+K+ across cell membranes. The use of D. purpurea needs strict control so that side effects can be minimized.
Isolasi dan Uji Patogen Bakteri Asam Laktat (BAL) dari Usus Ayam Kampung yang Dipelihara Secara Intensif Di Kefamenanu Welsiliana; Charles Venirius Lisnahan; Lukas Pardosi
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This study aimed to isolate LAB from the intestines of native chickens and to test the ability of LAB activity against pathogenic bacteria. Bacterial isolation was carried out by taking chicken intestines, then diluting up to 102. The results of the dilution were taken 1 ml to be inoculated into MRSA media using the pour plate technique, streak plate technique was used to obtain pure cultures and then incubated at 37˚C for 24 hours. LAB culture testing for pathogenic bacteria was carried out using MHA media containing Escherichia coli cultures, then incubation for 120 hours at 37˚C. Observation parameters included colony shape, colony color, number of LAB colonies, the diameter of the clear zone and LAB colonies. The results showed that the bacteria isolated from the intestines of native chickens were LAB which had around colony shape and were yellowish-white (beige)-milky white. The number of LAB colonies obtained was 3.7 x 105 CFU/ml with the best inhibitory ability of pathogenic bacteria found in USA 3 isolates (1.85 cm clear zone; 0.90 cm colony size) after incubation for 120 hours.
Eksplorasi Fungi Endofit dari Tanaman Sambung Nyawa (Gynura procumbens (Lour.)Merr.) Ranti; Ummi Hiras Habisukan; Amin Nurokhman
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Sambung nyawa (Gynura procumbens(Lour.) Merr.) is a plant that can be used as herbal medicine such as healing cuts, lowering blood sugar levels, facilitating menstruation, and preventing cancer formation. The various properties possessed by sambung nyawa plants make these plants continue to be used and experience plant scarcity. Another alternative is to use endophytic fungi which are known as a source of efficacious medicinal secondary metabolites but have not been widely used. This study aims to isolate and identify the genus of endophytic fungi on sambung nyawa plants. The method used in this study was sampling, namely root organs, root bark, stems and leaves of sambung nyawa plants, making media using instant PDA media mixed with aquadest and antibiotics, endophytic fungi using direct planting techniques on PDA media in cups. petri, purification of endophytic fungi by eliminating fungi that are considered to be the same from their morphological characteristics until truly pure fungi are found, endophytic fungi help macroscopically by observing the color, texture, and pattern of the colony then observing microscopically using the culture slide technique then observing under a hyrox microscope by observing the type of spore, spore shape and hyphae. The results of this study were 12 isolates of endophytic fungi identified in 10 different genera, namely Nigrospora, Blastomyces, Fusarium, Pythium, Cladosporium, Trichoderma, Gliocladium, Papulaspora, Aspergillus, Monacrosporium.
Peranan Mikroorganisme Pendegradasi Plastik: Tinjauan Biodegradasi Plastik, Mekanismenya, serta Mikroorganisme yang Berperan Putu Cindy Arista
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Plastic is difficult to decompose in nature because it is a synthetic polymer made by humans and is made from petroleum. The problem that arises from the uncontrolled use of plastic is the accumulation of plastic waste in the environment which is not handled properly and becomes a source of environmental pollution. Plastic waste creates various kinds of environmental problems that disturb the balance of ecosystems and have an impact on the life of organisms in the environment. To overcome this problem, it is necessary to carry out related research as an effective solution for dealing with plastic waste. Biodegradation is one of the right methods for dealing with plastic waste now and in the future. However, there is limited review of the bio-decomposition of plastics, the microbial species present, the effectiveness of degradation, and the mechanisms involved. Based on this, this research focuses on a brief review of plastic biodegradation such as the factors that affect biodegradation, the species of microorganisms that play a role in the degradation of macro and micro-plastics, as well as the mechanism of plastic degradation through enzymes from the viewpoint of microorganisms. Various species of microorganisms such as bacteria, algae and fungi have the ability to decompose plastics but they cannot mineralize plastics completely. Meanwhile, research on the enzymes involved in plastic degradation is still limited, especially in the case of algae. This review can be a reference for researchers to find effective strategies to address the problem of plastic waste worldwide.

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