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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 2,125 Documents
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SELEKSI JAMUR TANAH PENGURAI LIGNIN DAN PAH DARI BEBERAPA LINGKUNGAN DI BALI [Selection of Lignin and PAHs Degrading Fungi from Some Environment in Bali] YB Subowo; Corazon Corazon
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1976

Abstract

An investigation on the selection of soil fungi that degrade lignin and PAHs from some environment in Bali had been done. Some soil fungi are able to degrade lignin compound and PAHs. This fungus can be used to degrade industrial waste containing lignin and hydrocarbon compound. The aim is to obtain fungal capable of degrading lignin and hydrocarbon compounds.The soil samples were taken from mangrove areas, beaches and organic farms to the laboratory for fungal isolation.The isolation of the sample was obtained 20 number isolates. Penicillium sp KSt3 is the highest yield of mycelium.This fungus has ability on poly R-478 degrading as much as 2.3% within 60 minutes. This fungus has laccase activity of 3,32 units/ml and capable of degrading phenanthren (PAH) as much as 85.8% in 8 days.
KEEFEKTTFAN BEBERAPA BIAK RHIZOBIUM SEBAGAI INOKULAN PADA PERTUMBUHAN ALBIZIA PROCERA DAN A. SAPONARIA HJD. LATUPAPUA; S.H. RAHAYU; S. ABDULKADIR
BERITA BIOLOGI Vol 3, No 8 (1988)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i8.1310

Abstract

HJ.D. LATUPAPUA,S.H. RAHAJU & S.ABDULKADIR.1988. The effectiveness of some rhizobial strains as inoculants on the growth of Albizia procera and A. saponaria.Berita Biologi 3(8) 405 - 412.Four experiments have been carried out under green house conditions,using 0.5 gallon plastic pots, filled with 1.5 kg of sterile sands.The experimental design was completely randomized design with five replications.The first and second experiments were implemented on A.procera which consisted of 11 and 9 strains, lasted in 10 weeks respectively.The third and the fourth used 7 strains for each experiment,were implemented on A.saponaria,lasted in 12 and 16 weeks respectively.The results showed that all inoculated plants produced root nodules. In the experiments I and H,good results were demonstrated by strains number 34, 85 and 119, while strain Rsg showed very poor results.Rhizobium which showed poor results were strains Raf 1,Raf 2, Rap, Rss, Ri and Le. In the experiments III and IV, good results were demonstrated by strains 82 and 85,while very poor results were shown by strains Cg and Ri, poor results were shown by strains 34, 119 and Le.
POTENSI ANTIBAKTERIA EKSTRAK DAN FRAKSI LIBO (Piper miniatum BL) Sumarnie -H Priyono
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.808

Abstract

Libo (Piper miniatum Bl.) is traditionally used in Papua as spices and tonic. Besides as food flavor, it is commonly used as food natural preservative that is related to its antibacterial activity. But so far, there is no scientific report about antibacterial activity from the plant extract.The antibacterial activity test result in this study, showed that the four extracts (n-hexane, chloroform, ethyl acetate and methanol) of libo were active against Streptococcus mutants (BCC1863) and Staphylococcus aureus (ATCC 25923). The extracts were active only on high consentration againts Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 25922), meanwhile on Salmonella enteriditis (BCC 2754), Staptylococcus epidermidis strain Cowan (BCC 1820) and Bacillus subtilis (ATCC 10400) gave different antibacterial effect. The n-hexane extract has the highest antibacterial activity againts S. mutans (BCC 1863). Fractionation result of n-hexane extract, F3 and F4 at concentration 124.4|ig showed antibacterial effect against Listeria monocytogenes (M 19117) equivalent to erythromycin 10 \\g as standart antibiotic. While Fl and F2 at concentration 124.4|ig had antibacterial activity against E. coli (ATCC 25922) equivalent to erythromycin 10 ug.
PERTUMBUHAN, PRODUKSI DAN POTENSI GIZI TERONG ASAL ENGGANO PADA BERBAGAI KOMBINASI PERLAKUAN PEMUPUKAN Titi Juhaeti; Peni Lestari
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2246

Abstract

This research was carried out to study growth, production and nutritional potential of two accessions of Enggano eggplant on various combinations of organic and anorganic fertilizer. The experiment was designed using to Factorial Randomized Complete Block with 5 replications. The first factor was two eggplant accessions (Meok accesion/round fruit and Malakoni accession/long finger fruit). The second factor was 9 combinations of organic and inorganic (NPK) fertilizer. The result showed that long finger eggplant more productive than rounded eggplant. The total fruit fresh weight of long finger eggplant (2383.3 g) higher than rounded eggplant (1016.4 g). The ½ NPK + ½ StarTmik fertilization treatment showed better vegetative growth than other treatments. Meanwhile, for total plant production, NPK treatment showed the highest fruit production followed by ½ NPK +½ POKomersial and ½ NPK +½ starTmik treatments.  Nutritional analysis showed that long finger eggplant contained of vitamin C 15.887 g/100g, phosphor 25.983-27.440 mg/100g; calcium 12.340-13.274 mg/100g; fat 0.120-0.277%, carbohydrate 6.244-8.315% and crude fiber 0.872-1.110%. Meanwhile, round eggplant,  contained of vitamin C 10.354 g/100g, phosphor 34.919-37.012 mg/100g; calcium 14.355-15.240 mg/100g; fat 0.275-0.338%,  carbohydrate 6.733-7.896% and crude fiber 2.005-2.211%.
POTENSI SUMBER DAYA HAYATI SEBAGAI PENUNJANG PEMBANGUNAN DAERAH YANG BERKELANJUTAN Aca Sugandhy
BERITA BIOLOGI Vol 5, No 5 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i5.1451

Abstract

Konsep perabangunan berkelanjutan (sustainable development) di Indonesia selatna ini belum diimplementasikan sebagaimana mestinya.Pembangunan yang dijalankan selama ini masih menitikberatkan pada aspek ekonomi dan meninggalkan aspek konservasi jauh di belakang. Akibatnya adalah menurunnya kualitas lingkungan hidup yang terjadi di mana-mana, termasuk degradasi lahan, penurunan fiingsi dan produktivitas lahan serta meningkatnya berbagai bentuk bencana alam. Untuk itu kebutuhan manusia dan kegiatannya haras diselaraskan dengan pemeliharaan keanekaragaman hayati. Pendekatan keterpaduan ini dijabarkan dalam pengelolaan bioregional yang mewadahi dan menitikberatkan konservasi keanekaragaman hayati dan peran serta masyarakat dalam memanfaatkan sumberdaya alam.
PERBANYAKAN DAN PENYIMPANAN KULTUR SAMBUNG NYAWA [Gynura procumbens (Lour.) Merr.] DENGAN TEKNIK IN-VITRO Djadja Siti Hazar Hoesen
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1122

Abstract

Sambung nyawa [Gynura procumbens(Lour.)Merr.]is one of the traditional medicine sources.The plants are not cultivated intensively in the field. One of the efforts to maintain and to propagate this species is by multiplication through tissue culture method. Nodes explants were cultured in MS normal and half strength concentration macroelements, supplemented with microelements and vitamins; combination of cytokinin BA (2 mg/1), thidiazuron (0.01 mg/1 and 0.1 mg/1) and adenine sulphate (5 mg/1).Auxin (2,4D 0.5 mg/1) in combination were added in media as treatments and activated charcoal (2 g/1) as antioxidant. Young leaves explants were also cultured in the same basic medium (MS and 'A MS) in treatments with cytokinin (BA and thidiazuron).The results from nodes and young leaves explant indicated that the highest number of survival cultures were obtained from combination between BA (2 mg/1) and thidiazuron (0.01 mg/1) in MS normal strength basic medium.In acclimatization stage, 100% of plantlets survived and successfully transplanted to soil medium in the field; maintenance study indicated that subculture was prolonged until 52 weeks.
GEJALA KLINIS DAN PATOLOGI ANATOMI PASCA INFEKSI Campylobacter jejuni PADA AYAM BROILER Andriani Andriani; Mirnawati Sudarwanto; Surachmi Setiyaningsih; Harsi Dewantari Kusumaningrum; Herwin Pisestyani
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i3.498

Abstract

Campylobacter spp. is a bacteria foodborne zoonotic agent that can infect humans and animals, especially birds. This bacteria can cause important problems in the public health since the beginning at the farm level, the level of retail and on the table. Poultry meat is the main source of contamination because the digestive tract of poultry is the predilection of Campylobacter jejuni. During this infection C. jejuni in poultry did not show typical clinical symptoms, making detection quite difficult to diagnose this disease at the farm level. Incidence infection of Campylobacter sp. in developed countries had 5-90%. Currently poultry production and consumption are predicted to still increase due to a high demand for low price protein. This research is conducted to determine the clinical and pathologic anatomy due to an infection of C. jejuni on chicken digestive tract. A total of 105-day-old chickens are divided into 3 groups, each consisting of 35 individuals. Group A was the control group (not infected) group B (infected with C. jejuni ATCC 33291), and group C (infected with field isolate of C. jejuni/C1). Infection of the chicken is done 0.5 ml peroral suspension concentration of104cfu/ml. Anova Duncan is used to analyze parameters of body weight, increase weight gain, feed consumption, FCR values. Kurskall Walis method used to analyze the parameters non-parametric form of the score changes in the macroscopic and microscopic intestinal and liver. Infection of C. jejuni changes the pathology anatomy of intestine i.e. hyperemia, enteritis and enteritis catharalis haemorrhagic. A microscopic change of intestinal is oedema,haemorrhage and infiltration of inflammatory cell. Changes of pathology anatomy liver are colour smeary, congesti, pale, fragile and necrotic. Microscopic changes of liver are degeneration and focal hepatic necrosis. Group observation infected by C. jejuni caused chicken cannot reach maximum body weight and can be detrimental to farmers because of increase of the value of the feed conversion ratio (FCR).
AKTIVITAS ANTIBAKTERI EKSTRAK KULTUR JAMUR ENDOFIT Fusarium sp. CSP-4 YANG DIISOLASI DARI Curcuma sumatrana Miq. Dewi Wulansari; Ersaliany Nurul Pratiwi Qodrie; Bodhi Dharma; Andi Saptaji Kamal; Lukman Hafid; Lina Marlina; Praptiwi Praptiwi
BERITA BIOLOGI Vol 19, No 1 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i1.3350

Abstract

Endophytic fungus Fusarium sp. CSP-4 was isolated from the rhizome of Curcuma sumatrana Miq. The ethyl acetate extract of CSP-4 was fractionated by chromatographic column with silica as a stationary phase. The fractions were tested for antibacterial activity against S.aureus and E.coli by Thin Layer Chromatography (TLC)- bioautography. Determination of Minimum Inhibitory Concentration (MIC) was carried out by the microdilution method on a 96-microwell plate with the concentrations from 2 to 256 µg/ml. The results showed that fraction 15 and 11 were able to inhibit bacterial growth with the MIC values of 256 and> 256 μg / mL against S.aureus and>256 μg / mL against E.coli, respectively.The antibacterial activity of fractions 15 and 11 was weaker compared to the positive control of chloramphenicol with the MIC value of 4 µg/mL against S.aureus and E.coli. Based on the results obtained showed that the fractions of the methanol extract of endophytic fungus Fusarium sp. CSP-4 have weak antibacterial activity. 
RESPON PERTUMBUHAN DAN KETERGANTUNGAN Albizzia saponaria (LOUR.) MIQ TERHADAP INOKULASI FUNGI MIKORIZA ARBUSKULA LOKAL SULAWESI TENGGARA PADA MEDIA TANAH PASCA TAMBANG NIKEL Faisal Danu Tuheteru; Husna Husna; Asrianti Arif
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i5.1917

Abstract

This study that so far has not been conducted yet by others, regarding the effect of a local AMF species from Southeast Sulawesi on the growth of Albizzia saponaria (Lour.) Miq. The effect of AMF to the plant growth depends on the compatibility between host plant and fungi species which was controlled by genotypes of the both symbionts. The aim of this research is to know the growth respons and dependency of A. saponaria to this local AMF grown in post-nickel mining soil. This research was conducted in the Screen House of Forestry Department, Faculty of Agriculture-Haluoleo University since June to September 2010. A completely randomized design with three replications was used. The treatments were no inoculation of AMF (A), inoculated with -1 -1AMF Mycofer inoculum 5 g polybag (B), and inoculated with local AMF inoculum 10 g polybag (C). The seedling parameter observed were height, diameter, number of leaf, total biomass, number of nodule, percentage of root colonization and relative -1 mycorrhizal dependency (RMD). Results showed the increasing growth and biomass with the application of AMF 10 g polybag (C). There was a positive-strongest correlation value (P<0.01) between percentage of root colonization to all of seedling growth parameters, except to the diameter which was only strong correlated (P<0.05). A. saponaria seedling has high dependency level on local AMF for their survival in heavy metal contaminant soil.
KAJIAN KERAGAMAN GENETIK BURUNG KAKATUA TANIMBAR (Cacatua goffini, Finsch) MENGGUNAKAN PENCIRI "RAPD" Dwi Astuti; Siti N. Priyono; Heddy Julistiono; Dedy Duryadi
BERITA BIOLOGI Vol 4, No 2&3 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i2&3.1277

Abstract

The study was conducted to analyse genetic diversity of Goffini Finsch Cockatoo (Cacatua goffini Finsch) bird using RAPD (Random Amplified Polymorphic DNA) marker.PCR (Polymerase Chain Reaction) was performed on DNA samples extracted from 14 birds,using 18 random 10-merprimers and 2 random 12-merprimers. Fourteen out of 20 primers (70 %) successfully amplified DNAfragmens and 11 out of 14 primers (78,57 %) generated 1-2 specific alkies.The result clearly demonstrated that the RAPD marker allows for genetic diversity analyses of these birds in efficiently. Tree of relationship among 14 birds showed that there were two groups in the population ofGoffin 's Cockatoo.

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