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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 2,125 Documents
 202   203   204   205   206 
PENGARUH MINYAK ATSIRI DAUN JERUK PURUT (Citrus hystrix) TERHADAP DINDING SEL BAKTERI Staphylococcus aureus Opstaria Saptarini; Ismi Rahmawati
BERITA BIOLOGI Vol 20, No 1 (2021)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i1.3976

Abstract

Kaffir lime leaves essential oil has activity to inhibit bacterial growth. The mechanism of the inhibition is thought to be located in thebacterial cell wall. This study aimed to determine the antibacterial work location of kaffir lime leaf essential oil against Staphylococcus aureus ATCC 25923. Kaffir lime leaves were distilled by steam distillation, essential oils obtained were identified by organoleptic quality, evaporation, solubility in alcohol, refractive index and specific gravity. The results were compared under with the literature. The results of essential oils were made with various concentrations of 100, 50, 25, 12.5, 6.25, 3.13, 1.56, 0.73, 0.39, and 0.20%. The results of the concentration series were tested for antibacterial activity with the macrodilution method followed by the determination for the location of bacterial growth inhibition using the Scanning Electron Microscope (SEM). The yield of kaffir lime leaf essential oil was 0.82%. The observation of organoleptic essential oil has a light yellow color, the distinctive odor of kaffir lime, a liquid form, and distinctive lime taste. Refractive index examination resulted 1.454; specific gravity 0.8317, and soluble in 70% ethanol with a ratio of 1:1. The Minimum BactericidalConcentration (MBC) obtained from the antibacterial activity test was 6.25%. Kaffir lime leaves essential oil mechanism occurred in the cell walls and cell membranes of Staphylococcus aureus. 
EFEK AROMATERAPI MINYAK ATSIRI MAWAR (Rosa damascena MILL.) DAN KULIT JERUK LIMAU (Citrus amblycarpa) TERHADAP JUMLAH MIKROBA UDARA RUANGAN BERPENDINGIN Oom Komala; Novi Fajar Utami; Siti Mariyam Rosdiana
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3697

Abstract

Energy, material, and or other components were entered to the air by activities of human could cause air quality down to a specific level that could affect human health. The bacteria in the human oral cavity such as Staphylococcus sp. were found in the air during common activities such as coughing, sneezing, and speaking. The research aimed to assess the influence of the combination of oils essential of Rosa damacena and leather oil of Citrus amblycarpa against a decline in the total of microbes on air-conditioned rooms. The method of the research  was used the evaporation of aromatherapy and plate count method. The results were showed that P3 with the concentration of oils essential of R. damacena 2% and leather oil of C. amblycarpa 5% most effective could decrease the total of microbes with an average of 72.5%. Organoleptic, relative density, refractive index, solubility in 90% ethanol, and acid number analysis of Rosa damacena and Citrus amblycarpa leather essential oils met the Indonesian National Standard requirements except for the acid number of leatherlime essential oil and the optical rotation of rose essential oil. It is concluded that the combination of oils essential R. damacena and leather oil of C. amblycarpa  could reduce the total of microbes on air-conditioned rooms.
PERILAKU SINYAL AKUSTIK DAN VISUAL DARI KATAK JANTAN Staurois gutattus DI GUNUNG POTENG KALIMANTAN BARAT Mohamad Jakaria; Junardi Junardi; Riyandi Riyandi
BERITA BIOLOGI Vol 19, No 3B (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3B.3960

Abstract

The behavior of both acoustic and visual signals has been observed in Staurois gutattus black-spotted rock frogs. The aim of the study was to determine how S. gutattus frogs communicate to find their mates through acoustic and visual signals. This research was conducted in Mount Poteng, West Kalimantan. Data were analyzed descriptively and graphically to explain the relationship between the measured parameters. Data collection of advertisement call, foot-flagging and amplexus behavior is done directly in the field. All activities carried out are recorded with the camera. The duration of each part of the breeding activity is also calculated using a stopwatch. The male frog's advertisement call and foot-flagging are carried out by the male frogs while attracting their mates to mate. Where the foot-flagging activity was mostly carried out on the rock substrate (62%), while on the leaf substrate advertisement call was more dominant (47%). Then based on the duration, activity advertisement call, foot-flagging and amplexus the longest duration was performed by male frogs in the time range 18.00-05.59. Meanwhile, from the number of individuals, the advertisement call, foot-flagging and amplexus activities were mostly carried out at 18.00-05.59.   
ANTIFUNGAL EXTRACT ACTIVITYOF ISOLATE Nocardia sp. ATS-4.1 AGAINST Candida albicans InaCC-Y116 Abdullah Abdullah; Rahmawati Rahmawati; Rikhsan Kurniatuhadi
BERITA BIOLOGI Vol 19, No 3A (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3A.3868

Abstract

Candida albicans is a microorganism that knows as caused of candidiasis. Nocardia is known to have the ability to produce antifungal bioactive compounds to overcome cases of fungal infections. This research aims to determine the presence of antifungal activity and the good concentration from crude extract of Nocardia sp. ATS-4.1 to inhibit C. albicans InaCC-Y116 and. Antifungal activity test of isolate Nocardia sp. ATS-4.1 was performed using a well diffusion method on Mueller Hinton Agar (MHA) media with a concentration of 92%, 94%, 96%, 98%, 100% and nystatin (positive control) 0,0125% and DMSO 10% (negative control) incubated at 37 ᵒC for 24-48 hours. The results showed that the extract of Nocardia sp. ATS-4.1 can inhibit the growth of C. albicans. Nocardia sp. ATS-4.1 isolate extract concentration of 96% with a resistance diameter of 13.63 ± 0.53 mm with a strong category against the growth of  C. albicans InaCC-Y116.    
KAJIAN AWAL POTENSI OPOSUM LAYANG (Petaurus breviceps) SEBAGAI RESERVOIR BAKTERI ZOONOTIK DAN RESISTENSI ANTIMIKROBA Rifka A. N. Safitri; Sarsa A. Nisa; Nurul Inayah; Taufiq P. Nugraha; Agung Suprihadi; Sri Pujiyanto; Anang S. Achmadi; Achirul Nditasari; Sugiyono Saputra
BERITA BIOLOGI Vol 20, No 1 (2021)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i1.3974

Abstract

Oposum layang atau sugar glider (Petaurus breviceps) merupakan salah satu satwa endemik Indonesia. Permintaan akan satwa eksotis ini sebagai hewan peliharaan terus meningkat namun informasi terkait potensi zoonosis yang ditimbulkannya masih sangat terbatas. Penelitian ini bertujuan untuk mendeteksi dan mengidentifikasi bakteri patogen yang dibawa oleh oposum layang melalui pendekatan culture-dependant method dan untuk mengetahui pola resistensi antibiotiknya. Sampel yang digunakan adalah feses oposum layang (n=21) yang dikoleksi dari fasilitas riset satwa liar di Pusat Penelitian Biologi LIPI, Bogor. Berdasarkan uji presumptif Salmonella pada medium Xylose Lysine Deoxycholate (XLD) agar, sebanyak 6 sampel (29%) dinyatakan positif, sedangkan  uji presumtif untuk Listeria pada Listeria isolation transwab dinyatakan positif untuk semua sampel (100%). Secara total, sebanyak 43 isolat telah berhasil dikoleksi dan dikarakterisasi fenotipiknya terhadap antibiotik dan sembilan isolat (21%) diantaranya menunjukkan adanya resistensi terhadap satu jenis antibiotik atau lebih. Sementara itu, tiga isolat potensial patogen telah diidentifikasi menggunakan gen 16S rRNA yaitu Shigella sonnei (X15), Klebsiella pneumoniae (X21) dan Bacillus flexus (H8). Penelitian lanjutan untuk mengidentifikasi bakteri yang dikoleksi dan mengkonfirmasi patogenisitasnya masih perlu dilakukan namun berdasarkan hasil dari kajian awal ini, kami menguatkan hipotesis bahwa oposum layang berpotensi sebagai reservoir dari bakteri zoonosis sekaligus reservoir dari resistensi antimikroba. 
AKTIVITAS ANTIBAKTERI AIR PERASAN DAN REBUSAN DAUN CALINCING (Oxalis corniculata L.) TERHADAP Streptococcus mutans Ni Luh Arisa Prahastuti Winastri; Handa Muliasari; Ernin Hidayati
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3786

Abstract

Inappropriate use of antibiotics can cause bacteria to become resistant and have side effects, therefore an alternative source of antibacterial is needed. One species of plant that has antibacterial properties is calincing (Oxalis corniculata L.). Antibacterial activities of the juice and decoction of calincing leaves against Streptococcus mutans have not reported yet. This study aims to determine the antibacterial activity of the juice and decoction of calincing leaves against Streptococcus mutans. This study uses a well diffusion method with eight treatment groups, namely juice and decoction at the concentrations of 20%, 40%, 60%; listerin 100% as a positive control, and distilled water as a negative control. The diameter of the inhibitory zone of the juice and decoction of calincing leaf at a concentration of 20% are 19 mm and 24.33 mm respectively, and classified as strong growth inhibition, while the concentrations of 40% and 60% are classified as very strong growth inhibition. Growth inhibition of the juice and decoction of calincing leaf at concentration of 20% was significantly higher than the positive control. The best antibacterial activity against Streptococcus mutans is produced by the juice and decoction of calincing leaf at concentration of 60%. Increasing in the concentration of the juice or decoction of calincing leaf also increases the diameter of the inhibitory zone.   
ISOLASI DAN KARAKTERISASI ISOLAT BAKTERI RESISTEN TEMBAGA DARI SUNGAI CISADANE Wahyu Irawati; Candra Yulius Tahya
BERITA BIOLOGI Vol 19, No 3B (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3B.3937

Abstract

The water quality of the Cisadane River, located in the industrial factory area, it is contaminated with copper, it is necessary to treat sewage. Biological wastewater treatment using bacteria is a more economical and effective method of removing copper from the environment than chemical methods. This study aims to isolate, identify, determine the level of resistance, and analyze the ability of bacteria to accumulate copper. Isolation was carried out by the spread method in Luria Bertani Agar medium containing CuSO4. Bacterial isolates were identified based on analysis of the 16S rDNA gene. The resistance level is determined based on the Minimum Inhibitory Concentration (MIC) value. The accumulation ability of copper was determined using an atomic absorption spectrophotometer. Six bacteria resistant to copper were isolated and identified as Pantoea agglomerans strain IrCis1, Klebsiella grimontii strain IrCis3, Klebsiella penumoniae strain IrCis4, Shigella flexneri strain IrCis5, Enterobacter cloacae strain IrCis6, and Enterobacter cloacae strain IrCis9 with MIC of 7 mM - 9 mM. Pantoea agglomerans strains IrCis1, Klebsiella grimontii strains IrCis3, Klebsiella pneumoniae strains IrCis4 can accumulate copper of 1,19 mg, 1,39 mg, and 0,92 mg, respectively, per gram of dry cell weight.  
JAVANESE ENDEMIC STROBILANTHES(ACANTHACEAE): TAXONOMY, DISTRIBUTION AND CONSERVATION STATUS Yasper Michael Mambrasar; Yayah Robiah; Nira Ariasari Z.; Yayan Supriyanti; Dewi Rosalina; Sutikno Sutikno; Jaenudin Jaenudin; Wahyudi Santoso; Dede Surya; Megawati Megawati; Taufik Mahendra; Agusdin Dharma Fefirenta; Deby Arifiani
BERITA BIOLOGI Vol 19, No 3A (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3A.3992

Abstract

The taxonomic status of Javanese Strobilanthes has been revised by Bennett and Scotland (2003). However, the results of the validation of the Javanese Acanthaceae by Girmansyah (2014) show that several Strobilanthes species, especially those endemic to Java, need to be revised. Because they have become synonymous. Conservation status of Javanese endemic Strobilanthes was carried out according to the IUCN Red list Categories and Criteria.  
KERAGAMAN LUMUT KERAK PADA TANAMAN TEH (Camellia sinensis (L.) Kuntze) DI PERKEBUNAN TEH PT. SARANA MANDIRI MUKTI KABUPATEN KEPAHIANG PROVINSI BENGKULU Rochmah Supriati; Helmiyetti Helmiyetti; Dwi Agustian
BERITA BIOLOGI Vol 20, No 1 (2021)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i1.3944

Abstract

Lichen is a mutualism symbiotic organism between fungi (mycobiont) and photosynthetic symbiont in the form of algae (photobiont). It can be found from the lowlands to the highlands, growing epiphytically on soil, rocks, weathered wood, and tree bark, as shown on surface of the tea plants (Camellia sinensis (L.) Kuntze) in The PT Sarana Mandiri Mukti Tea plantation in Kepahiang regency, Bengkulu Province. The purpose of this research was to identify and find out the species of epiphytic lichens on the tea plant in this place. The study was conducted in May–November 2019. Samples was collected purposively, by taken ephyphitic lichens growth on the bark of tea plants stems. Then, samples was identified based on morphological characteristics at the Basic Science Biosystematics Laboratory, FMIPA University of Bengkulu. The data obtained were analyzed descriptively. It was identified as many as 35 species of lichens from the Ascomycota division, belonged to three classes, six orders, 11 families; those are  Graphidaaceae, Stereocaulaceae, Parmeliaceae, Lecanoraceae, Malmideaeceae, Pertusariaceae, Teloschistaceae, Caliciaceae, Physciaceae, Arthoniaceae, dan Pyrenulaceae. 23 species have crustose type thalus and 12 species have foliose type thalus.  
SELECTIVE ISOLATION OF Dactylosporangium AND Micromonospora FROM THE SOIL OF KARST CAVE OF SIMEULUE ISLAND AND THEIR ANTIBACTERIAL POTENCY Ade Lia Putri; I Nyoman Sumerta
BERITA BIOLOGI Vol 19, No 3A (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3A.3933

Abstract

Karst is a unique ecosystem that consists of a thin soil layer on the carbonate rocks. It has a diverse microorganism, especially actinomycetes group, which might potentially produce beneficial secondary metabolites that remain unknown. In this study, we were interested in isolating Dactylosporangium and Micromonospora that have been potentially reported as antibiotic sources. We used two methods using the chemical germicide (pretreatment 1.5% phenol) compared with the SDS-YE (0.05% SDS pretreatment). Thirty-nine isolates actinomycetes were successfully isolated from two samples of the karst cave soils. Thirteen isolates were obtained by using the germicide chemical method and closely related to the genera of Dactylosporangium and Micromonospora. On the other hand, the SDS-YE grew twenty-six isolates which closely related to 11 genera of actinomycetes (Catenulispora, Nocardia, Rhodococcus, Ornithinimicrobium, Catellatospora, Dactylosporangium, Micromonospora, Streptacidiphilus, Streptomyces, Nonomuraea, and Streptosporangium). These results suggest that 1.5% of phenol pretreatment could preserve the genera of Dactylosporangium and Micromonospora, while the Streptomyces and other rare actinomycetes were killed. Among all isolates, only 7 showed the antibacterial activity on tested bacteria. Even though the antibacterial activity of those isolates was not high, the isolation of actinomycetes from a specific substrate is necessary to be conducted on exploring the richness of our natural resources.  

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