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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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STUDI ETNOEKOLOGI MASYARAKAT DANI-BALIEM DAN PERUBAHAN LINGKUNGAN DILEMBAH BALIEM, JAYAWIJAYA, IRIAN JAYA Purwanto, Y
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The objective of the ethnoecology study of the Dani-Baliem society is to know the relationship between Dani-Baliem society and their environment.This study covers the perception and traditional management system of environment by the Dani-Baliem society, and also the impact of their activities to the environment change in Baliem Valley. This study is expected to determine the change of plant diversty, which happened, and to reconstruct this change from pre-historic era to the present time.The methods used in this study based on anthropological, ecological, paleoecological and bibliography study.The result showed the ecosystem change in the Baliem valley since 26.000 BP.It was indicated by Carbon content as a result of fired activities in the base of valley sedimentation.Since 7000 years ago, the impact of human activities in the Baliem valley area is significant. It is indicated by organic material sedimentation.This environment change continue untill now and relate to the intensity of human activities intervention. These human activities cause many enironment units which has physical and biological characteristics. By using floristic analysis in each environment unit, it can be seen the change of plant diversity composition in this area.

EVALUASI ANTIBAKTERI DAN ANTIOKSIDAN EKSTRAK SMILAX spp. DARI PULAU ENGGANO [Evaluation of Antibacterial and Antioxidant of Smilax spp. Extracts Collected from Enggano] Tiwi, Praptiwi; Palupi, Kartika Dyah; Fathoni, Ahmad; Keim, Ary P; Royani, M Fathi; Effendi, Oscar; Agusta, Andria
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Three species of Smilax spp. (Smilax macrophylla, S. odoratissima and S. zeylanica) collected from Enggano island were evaluated for their potential as an antibacterial and antioxidant. Stems and leaves of three species of Smilax spp. were extracted successively with nhexane,chloroform, ethyl acetate and methanol. Antibacterial activity was evaluated by TLC-bioautography against Eschericha. coli InaCC B5 and Staphylococcus. aureus Ina-CC B4. The antioxidant activity was analyzed by DPPH free radical activity by bioautography method. The value of minimum inhibitory concentration (MIC) and IC50 of active extracts were done by serial dilution in 96- well microplate. The results showed that 20 extracts have antioxidant activity, 13 extracts inhibited the growth of S. aureus Ina-CC B4, and 14 extracts inhibited the growth of E. coli Ina-CC B5. MIC values of active extracts against S. aureus Ina-CC B4 were in the range of 128 - > 512 Âµg / ml, while the values of MIC against E. coli B5 Ina-CC were > 512 Âµg / ml. IC50 values of extracts that has antioxidant activity were in the range of 184.11-4549.34 mg/L.

KOMPOSISI KIMIA MINYAK ATSIRI Melodorum cylindricum (Maing. ex Hook.f & 1 Thorns), Litseajirma (Blume) Hook.f., Fl. Brit. Ind. DAN Callistemon lanceolatus D.C. Jamal, Yuliasri
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Steam distillation of Melodorum cylindricum leaves (176 gram), Litsea firma leaves (295 gram), L firma stem bark (1,590 gram) and Callistemon lanceolatus leaves (300 gram) yielded 0.15%, 1.68%, 0.23% and 0.4% essential oils respectively.The oils were analyzed using GC-MS methods. The results identified 71 components from the leaves of M. Cylindricum which p-myrcene is the highest (12,56%). Leaves and stem bark of L. firma contained 20 and 24 components which 2-undecanone (34,95%) and undecanone (29,57%) as the highest major components. The essential oil of C. lanceolatus identified 24 components and 1,8 cineol is the major component with the highest content, 67.37%.

PERBANYAKAN Heritiera javanica (Blume) Koesterm. SEBAGAI JENIS PENGHASIL KAYU PADA BERBAGAI INTENSITAS NAUNGAN DAN MEDIA PERTUMBUHAN [Propagation of Heritiera javanica (Blume) Koesterm. as Timber Tree Species Under Several Shade Intensities and Growth Media] Sahromi, Sahromi; Purwantoro, R. Subekti; Siregar, Hartutiningsih M.
BERITA BIOLOGI Vol 14, No 3 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i3.1823

Heritiera javanica is a timber tree species and their natural regeneration is scanty, thus propagation of this species is urgently required. This research was aimed to identify the effect of shade intensity and growth media on the survival rate and growth of seedlings. Experi- mental design used was completely randomized with two factors, namely the shade intensity and the media that consisted of several cate- gories. Parameters measured were survival rate and seedlings growth; height, number of leaves, and number of roots. Survival rate was analyzed using descriptive statistics. Seedlings growth (height, number of leaves, and number of roots) were estimated by using analysis of variance. Results showed that treatments using categories of several media categories that were placed in the shade of 25%, 50%, and 85% had a high survival rate. In addition, seedlings that were placed in 50% shade grew better and optimally. Media of TK (soil + compost), TS (soil + chaff), and TSPk (soil + manure + chaff) that were placed on the 50% shade showed the best result to height of seedlings growth. Reintroduction needs to be done for the enrichment of H. javanica populations in nature.

C0-CULTURE OF AMYLOLYTIC FUNGI Aspergillus niger AND OLEAGINOUS YEAST Candida orthopsilosis ON CASSAVA WASTE FOR LIPID ACCUMULATION [Akumulasi lipid oleh kultur campuran kapang Aspergillus niger dan khamir Candida orthopsilosis pada media limbah singkong] Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The objective of study was to evaluate the effectiveness of co-culture between amylolytic fungiÂ Aspergillus niger and lipid accumulating yeast Candida orthopsilosis for lipid accumulation on cassava waste. When grown in 5 % starch medium, all microbes was able to grow, but best growth was observed on Aspergillus niger, other microbes grew slower. Moderate growth was observed Â on Saccharomycopsis fibuliger.Â The two isolates were then selected for further studies. Aspergillus niger and Saccharomycopsis fibuliger were amylolytic microbes. The amylolytic activities were affected by temperature, pH and nitrogen sources.Â Optimum temperature and pH for enzyme production were 30Â°C and 7.0 respectively. Both yeast extract and sodium nitrate were good nitrogen sources for amylase production. On cassava waste, the highest biomass and total lipid content were obtained by co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis. Major lipid composition was oleic acids and strearic acids. The ability of co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis grew and accumulated lipid on cassava waste would suggest that these culture were candidate for biofuel production.

PEMBENTUKAN KALUS DAN EMBRIOGENESIS KULTUR PELEPAH DAUN DAN DAUN Caladium HIBRIDA Irawati, Irawati
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i5.876

Petiole and leaf cultures of Caladium hybrid were grown on MS medium+ lppm 2,4-D and transplanted to MS medium + 1 ppm NAA and then were grown on MS media with or without Kinetin and NAA.The greatest capacity of explants to produce callus was from the base cut and the top cut of the petioles as well as from the main vein of the leaf. Compact calli were grew further into adventive buds and plantlets.Through Scanning Electron Microscope,the development of adventive buds were observed grew from the parenchymatous cells and the vascular bundles.

KARAKTERISASI DAN STUDI STABILISASI a-AMILASE Bacillus licheniformis TVII.6 MENGGUNAKAN BAHAN ADITIF Lestari, Puji; Richana, Nur; Rosmimik, Rosmimik
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The limited stability of enzyme during long-term storage attributes to its reduced function. In this study, a-amylase from Bacillus licheniformis TVII.6 were formulated with different kind of additives for storage stabilization and better performance. Simultaniously, some minerals and calcium ion were applied to elucidate the inhibition and activation effects to a-amylase. Crude enzyme which was prepared by aceton precipitation was used for this stability test. It demosntrated that 10% of mannitol in citrate phosphate buffer gave the highest residual activity after 3 months of storage (98.5%). Calcium ion influenced the thermal stability of a-amylase and it gave optimum activity at 5 mM CaCl , thus the stability increased from 76.0%/90 C/2 hours to 114.8%/90 C/22 hours in comparison without calcium ions. Calcium ions (5 mM CaCl ) on the stability of a-amylase at 4 C also produced the 2highest residual activity, which remained 100% during 48 hours of incubation. Chemical compounds like FeSO , Na CO and EDTA 4 2 3acted as inhibitors, while (NH ) SO , CuSO , CoSO , MgCl and K HPO did not inhibit activity of a-amylase. NaOH and MnCl 4 2 4 4 4 2 2 4 2 compounds at low concentrations (2 mM) did not inhibit the enzyme but at 10 mM became a-amylase inhibitors. This amylase stability information is very important as a consideration when applying and storing the enzyme, thereby reducing the degradation a-amylase activity.

NEROLIDOL, KOMPONEN KIMIA AROMATIK TANAMAN TEH YANG JUGA DIPRODUKSI OLEH JAMUR ENDOFIT Schizophyllum sp. D [Nerolidol, an Aromatic Chemical Constituent of Tea Plant that Produce by an Endophytic Fungi Schizophyllum sp. D] Agusta, Andria
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Cultivation of an endophytic fungus Schizophyllum sp.D, isolated from a tea plant Camellia sinensis (L.) O.K. in liquid medium PDB, on a rotary shaker at 100 rpm at room temperature (26 - 30 oC) produced a major metabolite with a production capacity of 56.7 mg/L. Structure elucidation based on MS, NMR spectra and published data showed that metabolite is nerolidol. This chemical compounds is one of important aromatic chemical constituent that contribute to tea aroma and defense system in a tea plant.

KARAKTERISASI ENZIM PENDEGRADASI SENYAWA ASETONITRIL DALAM SEL Fusiarum solani AIII2 Subowo, YS
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

An acetonitrlie degrading-enzyme of Fusarium solani AIII2 from whole cells were studied. Decrease ofÂ acetonitrlie concentration and formation of its degraded products were determined by gas chromatography. Ammonia analysis was done by Nesslers method. Fusarium solani AIII2 degraded 1% (v/v)Â acetonitrlie and produced acetic acid and ammonia. Acetonitrlie was degraded by nitrilase, with a rate of 0.903 mol min. mg dry weight cells. Maximum nitrilase activity of fusarium solani AIII2 was observed.

KARAKTERISTIK MIKROBA RUMEN HASIL ISOLASI DARI FESES SEGAR RUSA TIMOR (Cervus timorensis) Artiningsih, Typuk; Semiadi, Gono
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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