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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : - EISSN : - DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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RETRANSFORMATION AND EXPRESSION OF RECOMBINANT VIRAL PROTEIN OF JEMBRANA JSU AND JTat (JSU AND JTat) IN pGEX SYSTEM [Retransformasi dan Ekspresi Protein Virus Rekombinan JSU dan JTat Penyakit Jembrana dalam Sistem pGex] Margawati, Endang T; Utama, Andi; Indriawati, Indriawati
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Genom virus penyakit Jembrana setidaknya memiliki 3 gen besar yang menyandi protein dan beberapa di antaranya diperlukan untuk replikasi virus. Protein JSU dan JTat diduga dapat menginduksi kekebalan yang protektif pada sapi Bali terhadap penyakit Jembrana sehingga keduanya sangat berpotensi untuk dipakai sebagai vaksin rekombinan. Penelitian ini dirancang untuk meretransformasi protein rekombinan JSU dan JTat ke dalam Escherichia coli menggunakan sistem pGEX. Konstruk JSU dan JTat dalam pGEX dikoleksi plasmidnya dengan metode miniprep dan kemudian diretranformasikan ke dalam E. coli strain BL21 dan DH5a. JSU dan JTat hasil retransformasi diekspresikan pada medium LB untuk skala produksi kecil dengan sistem pGEX. Hasil penelitian ini meminjukkan bahwa kedua JSU dan JTat hasil retransformasi ke dalam E. coli strain BL21 terlihat tumbuh lebih baik pada medium LB jika dibandingkan retransformasi ke dalam E. coli strain DH5a. Hasil retransformasi JSU dan JTat dikarakterisasi dan diidentifikasi dengan Western blotting dan tampak menunjukkan ukuran protein yang benar, yaitu protein rekombinan JSU berukuran 60kDa dan JTat berukuran 36,7kDa. Protein rekombinan JSU muncul dengan pita tunggal dan lebih jelas jika dibandingkan dengan protein JTat. Konsentrasi protein JSU sedikit lebih rendah (1,883 mg ml ) jika dibandingkan dengan JTat (l,981mg ml).Penelitian ini menunjukkan bahwa JSU pGEX masih tersimpan dan diekspresikan dengan baik, sementara JTat mungkin perlu dilakukan perakitan ulang untuk memantapkan ekspresinya.

KONDISI HUTAN MANGROVE DITELUK AMBON: PROSPEK DAN TANTANGAN Suyadi, Suyadi
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The destruction of mangrove forest constitutes one of the greatest threats to biodiversity and conservation of Ambon Bay.But,data and information of condition and potential of mangrove in Ambon Bay are lacking. We link three methods: remote sensing,Biological survey using transect, and sedimentation sampling to study the condition and potential of mangrove in Ambon Bay specially for biodiversity and sediment trapped. Remote sensing data showed that area of mangrove forest in Ambon Bay 34 ha and has declined dramatically in the past decade.The research recorded 8 species of mangrove; two are recorded as new species compared to the last survey.Based on data collected from four transects, mangrove forest in Ambon Bay is habitat for 8 species of mollusc and species of fish, crustacean, bird and epiphytes. Sediment analysis showed that mangrove forest 80% more effective to reduce sediment that come in to Ambon bay. Finally, the result indicated that condition of mangrove forest in Ambon Bay has been declined, nevertheless, have great potential for conservation of biodiversity and efficient in trapping sediment that come in to Ambon Bay.

KONSTITUEN KIMIA MINYAK ATSIRI TIGA JENIS TUMBUHAN FAMILI RUTACEAE Jamal, Yuliasri; Sulianti, Sri Budi
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Hydrodistilation process of Feroniella lucida, Clausena harmandiana and Swinglea glutinosa leaves, 0.15%, 0.25 % and 0.20% essential oils were obtained respectively. The GC-MS analysis results shows the essential oil of Feroniella lucida contains w-desil ethanoate (38.23%), 1-decanol (11.01%), 1-decanol acetate (10.82%), and the essential oil of Clausena harmandiana contains a-pinene (12.23%) and copaene (12.40%).On the other hand, the essential oil of Swinglea glutinosa contains isocariophylene (10.09%), 3,3,7,7-tetramethyl-5-(2-methyl-l-prophenyl)-tricyclo 4.1.0.02.4 heptane (21.80%) and nerolidol type 2 (21.34%).O

SELEKSI JAMUR TANAH PENGURAI LIGNIN DAN PAH DARI BEBERAPA LINGKUNGAN DI BALI [Selection of Lignin and PAHs Degrading Fungi from Some Environment in Bali] Subowo, YB; Corazon, Corazon
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1976

An investigation on the selection of soil fungi that degrade lignin and PAHs from some environment in Bali had been done. Some soil fungi are able to degrade lignin compound and PAHs. This fungus can be used to degrade industrial waste containing lignin and hydrocarbon compound. The aim is to obtain fungal capable of degrading lignin and hydrocarbon compounds.The soil samples were taken from mangrove areas, beaches and organic farms to the laboratory for fungal isolation.The isolation of the sample was obtained 20 number isolates. Penicillium sp KSt3 is the highest yield of mycelium.This fungus has ability on poly R-478 degrading as much as 2.3% within 60 minutes. This fungus has laccase activity of 3,32 units/ml and capable of degrading phenanthren (PAH) as much as 85.8% in 8 days.

OPTIMASI DAN KARAKTERISASI a-AMILASE DARI ISOLAT AKTINOMISETES YANG BERASAL DARI KALIMANTAN TIMUR Soeka, Yati Sudaryati
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Forty-one actinomycetes isolates from East Kalimantan held in Microbiology Division Collection-UPI, and their ability to produce a-amylase has been assessed. Those 41 number of actinomycetes isolates performed amylolytic activity as shown by clear zone areal after being poured with iodium solution. The bacteria produced high a-amylase when was grown in media containing starch soluble 2% the a-amylase activity in media containing 8.24 U/ml. The isolate (number 7) was the most active compared to another (number 100) and it was identified as Nocardia; the activity of this enzyme obtained was 12.93 U/ml (one unit activity is defined as mol of glucose produced per ml per minute). The maximum temperature for enzyme reaction was 40Â°C, optimum pH was pH 7.5 the a-amylase activity were 15.76 U/ml and 31.11 U/ml, respectively. From kinetic characterization study, it was found that enzyme showed Km and Vmax value of 7.62 % (b/v) and 71.10- umol/ml/minute respectively at condition of temperature 40Â°C, pH 7.5 and incubation time 10 minute.

PENGARUH MEDIA TUMBUH TERHADAP PERKECAMBAHAN BIJI TANAMAN "LO"{Ficus racemoca L. var. elongata (King) Barrer} Solikin, Solikin
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Tanaman "lo" (Ficus racemosa L.) termasuk suku Moraceae yang berpotensi sebagai tanaman buah dan obat. Beberapa bagian tanaman lo telah dimanfaatkan penduduk, seperti buahnya dapat dimakan, daunnya berguna untuk pengobatan diare (Anonim, 1995); ekstrak kulit kayu dilaporkan dapat untuk aglutinasi sel-sel darah putih pada penderita leukimia((Rojo et al., 1999).

INDUKSIKALUS DAN REGENERASI TUNAS PULAI PANDAK (Rauwolfta serpentina L.) Yunita, Rossa; Lestari, Endang Gati
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.807

In vitro culture can be applied for producing new genotype which is tolerant to biotic and abiotic or to incerase secondary metabolic content. To obtain the optimum result of variety improvement, regeneration system should firstly be found out.It is sufficiently difficult to regenerate pulai pandak (Rauwolfia serpentina L.). Hence, with this system, the improvement of R. serpentina with secondary metabolic content higher than the other. The mother stok of R. serpentina used in this experiment, belongs to the collection of BB-Biogen. Calli were produced from leaves and internodes which is cultured at medium MS contain 2.4-D (0, 1, 3,5, 7 mg/1) combined with caseine hydrolysate 3 mg/1. Regeneration medium was MS contain BA (0,5, 1 mg/1) combined with zeatin (0, 0.1 and 0.5 mg/1) and root formation used was three kinds of auxin (IBA, IAA and NAA). The result showed that inter nodels was better that leaves to callus induction. In this experiment, MS + 2,4-D 1 mg/1 + CH 3 mg/1 was the best medium to induct calli,while medium MS + BA 1 mg/1 + Zeatin 0,5 mg/1 + maltosa 3% to regenerate and MS + IBA lmg/1 for root induction.

EKSTRAKSI MINYAK NABATI SECARA FERMENTASI Soeka, Yati Sudaryati
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Method of extracting vegetable oil from peanut and soybean cream through enzymatic fermentation was studied. Creams were incubated with respective strains of Bacillus sublilis, Candida rugosa and Pseudomonas aerogenes separately under mild condition that allowed for production of oil at pH 4.5-6.5 and room temperature overnight. A considerably amount of oil could be extracted from media containing coconut and peanut cream, however, none from soybean cream.The oil recovery was about 10 to 30% from approximately 46 to 52% total fat contained in the respective substrates.Those oil were extracted by application of culture filtrate of B. subtilis and C. rugosa exhibited higher linoleic and Iinolenic acid (0.50 to 0.61 % and 0.31 to 0.32% respectively), compared to those were of P. aerogenes lipase (0.20% and 0.13%, respectively).Further investigation was aimed to study the capacity of microbial strains on enzymatic reaction of fatty acid to fatty acid ester by incubating extracted oil in the present of organic solvents.

STUDI ANATOMI DAUN JENIS-JENIS AVERRHOA DI INDONESIA UNTUK MEMPERTEGAS STATUS TAKSONOMINYA Sunarti, Siti; Rugayah, Rugayah; Tihurua, Eka Fatmawati
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Leaves anatomy of Averrhoa spp. in Indonesia have been studied for supporting morphological character which used to recognize four species: A. carambola, A. bilimbi, A. leucopetala and A. dolichocarpa. The letter two species have leave crowded terminally or near the top of stem similar to those leave of A. bilimbi, whereas the fruit shape similar to A. carambola.The result of this study indicated that, these four species showed differently in their thickness of lamina and epidermis cell as well.

ANALISIS PREDIKSI SEBARAN ALAMI GAHARU MARGA Aquilaria DAN Gyrinops DI INDONESIA Roemantyo, Roemantyo; Partomihardjo, Tukirin
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1972

A spatial analysis was applied to predict the natural distribution of agarwood producing taxa of Aquilaria and Gyrinops in Indonesia.This research was conducted using herbarium materials which deposited in Hebarium Bogoriense, Research Center for Biology-LIPI and field data collected by researcher within periods of 1992-2009. Time serial maps of 1:250.000 were used in this analysis such as Indonesia digital base map of 1990, province land cover and deforestation digital maps of 1989 and 2005.Every sites of herbarium collection was identified using digital Cyclopedia of Malesian Collectors for the name of village/place and date of collection. The coordinate of those sites comprises latitude, longitude and altitude was retrieved by query method using Indonesia digital geo-reference data. Tabulated data of every collection/data was created and then overlaid to the time serial land cover and deforestation maps using GIS software, to identify the recent condition of that area. The results show that horizontal natural distribution of Aquilaria was mainly occur in the western part of Indonesia, while Gyrinops in eastern part of Indonesia. Vertical distribution analyses of the Aquilaria and Gyrinops showed that in general both genera are mainly grown naturally in the low land areas less than 300 m asl. Spatial analyses using time serial land cover and deforestation maps indicated that low land areas less than 300 m asl. having very high risk on the land use changes, whereas increasing land status classification from six on 1989 to fifteen on 2005. More than 20 % of forested areas have been change into non forest area such as plantation, agricultural land, resettlements and open unproductive lands since 1989. Natural distribution of agarwood producing taxa horizontally and vertically on all of major islands and the conservation strategy were discussed in this paper.

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