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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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UJI DAYA HAMBAT DAUN SENGGANI (Melastoma malabathricum L.) TERHADAP Trichophyton mentagrophytees DAN Candida albicans Gholib, Djaenudin
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The in vitro study of" inhibition effect of senggani leaves (Melastoma malabathricum L.) ethanol extract on Trichophyton mentagrophytees and Candida albicans was conducted by diffusion and dilution method. The extract was diluted into 5%, 10%,15% and 20% for diffusion test, and 1%, 2%, 3%, 4% and 5% for dilution test on T. mentagrophytees, and 2%, 4%, 6%, 8%, and 10% on C. albicans. The results showed that the extract inhibited fungal growth. Agar diffusion test (agar wheel) was observed by diameter of inhibition zone around the wheel. At 20% extract dilution, the diameter mean was estimated 30 mm on T. mentagrophytees, and 21 mm on C. albicans. In dilution test, the result showed Minimal Inhibition Concentration (MIC) of the extract was 3% on T. mentagrophytees, and 6% on C. albicans. It is concluded that the extract effect of inhibition on T. mentagrophytees is greater than that on C. albicans.

OPTIMASI PRODUKSI FRUCTOSYLTRANSFERASE OLEH Aspergillw sp. WNIC Toharisman, Aris; Triantarti, Triantarti; Marantesa, Hendro Santoso
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Fructo Oligo Saccharides (FOS) are considered as biologically benefit, have been developed recently to be used as functional factors in healthy foods.FOS shows low cariogenicity, nondigestibility and proliferation of bifidobacteria in human intestinal tract and dietary fiberlike action. The aim of this research is to produce FOS from sucrose by using fructocyltransferase (FT-ase) from Aspergillus sp. Research on the production of FOS was the optimization of FT-ase production. Inoculum of selected Aspergillus was added into medium with various composition and incubation conditions. Enzyme solution was mixed with sucrose and incubated at various times, pHs, temperatures and agitations.The best parameter condition was based on the highest FT-ase activity. The results showed that production of FT-ase was affected by fermentation time, pH and incubation temperature. The carbon source tested permitted good growth and enzyme production where sucrose supported rather good enzyme production.It was obvious that enzyme production was not closely correlated with cell growth.The best fructo-oligosaccharide yield (20.53%) was achieved when 20 g/100 ml sucrose was utilized. Yeast extract was good nitrogen source for enzyme production. The best FT-ase activity was achieved when 1.2 g/100 ml yeast extract was utilized. Addition of mineral salt also enhanced enzyme production where 1 g/l magnesium salt gave the best cell growth and enzyme production.

KEANEKARAGAMAN TUMBUHAN HUTAN "GUNUNG LUMUT" KABUPATEN PASIR, KALIMANTAN TIMUR Wiriadinata, Harry
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.788

The diversity of forest plants found in three locations in Gunung Lumut Protected Forest, East Kalimantan had been studied in November 2005 in which 252 number of herbarium specimen have been collected, encompassing 194 of herbs and shrubs,33 of trees, 21 numbers of climbers and 4 numbers of orchids.Among the collections like some members of Begoniaceae are interesting for their limited distribution and endemicity.

KOMPOSISI JENIS KEPITING {Decapoda: Brachyura) DALAM EKOSISTEM MANGROVE DAN ESTUARI, TAMAN NASIONAL BALI BARAT Murniati, Dewi Citra
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Mangrove and estuary ecosystem has highly biodiversity of benthic fauna. Crabs are the common benthic fauna found in this ecosystem. West Bali National Park is one area in Indonesia that has a good condition of mangrove and estuary ecosystem.The present study was done to provide data of the species composition of crabs in West Bali National Park. Sampling was undertaken on June 2009 in Teluk Gilimanuk, Teluk Terima and Teluk Banyuwedang. From a total 411 of crabs specimens, the highest species number was found in Teluk Terima.

PENGARUH MUTAGEN ETIL METAN SULFONAT (EMS) TERHADAP PERTUMBUHAN KULTUR IN VITRO ILES-ILES {Amorphophallus muelleri Blume) Poerba, Yuyu S; Leksonowati, Aryani; Martanti, Diyah
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Amorphophallus muelleri Blume (Araceae) is one of 27 Amorphophallus species occur wild in Indonesia (Sumatera, Java, Floresand Timor). The species is valued for its glucoman content for use in food industry (heathy diet food), paper industry, pharmacyand cosmetics. The cultivation of A. muelleri is hampered by limited genetic quality of seed. The species is triploid (2n=3x=39),the seed is developed apomictically. and pollen production is low. The species is only propagated vegetatively. This may explainthat the species is difficult to breed conventionally and genetic variabillity in the exiting landaraces cultivars is rather limited.Induced mutation using ethyl methan sulfonate is one of techniques to increase genetic variation. The present research is aimed todetermine Lethal Dosage (LD) 50% and 75% of EMS and to study effects of EMS on growth of A, muelleri in vitro cultures for usein induced mutation program. Results of the experiment showed that LD-50 and LD-75 was observed at 0.875% EMS and 0.5%EMS. respectively. Number of shoot, and percentage of rooting culture were decreasing as EMS level concentration increases.

HUBUNGAN TRANSPIRASI DENGAN HASIL DAN RENDEMEN MINYAK BIJI JARAK PAGAR (Jatropha curcas L.) Bora, Charles Y; Sulistyono, E; Yahya, S; Mahmud, Z
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The objective of this research was to evaluate the relationship between transpiration, yield and seed oil content of jatropha/jarak pagar (Jatropha curcas L.). The research was conducted at Cikabayan Research Garden of IPB July 2007-July 2008.The experimental design was Randomized Block Design with two factors and three replications. The first factor was genotype of Jatropha: IP-1A, IP-1M and IP-IP. The second factor was irrigation frequency: 7, 14, 21 and 28 days.The observed variables were evapotranspiration and yield component.The results show that the irrigation frequency of 7 days gave a higher evapotranspiration and transpiration rate than 14, 21 and 28 days irrigation frequencies. The differences affected yield component of each genotipe. The interaction effects between genotype and irrigation frequency were fruit number per plant. The transpiration positively and strongly related to fruits number, seed productions and oil production. Increased transpiration was followed by increasing on fruit number 0.014 (0.112 fruits/1/plant) for IP-1A and 0.024 (0.183 fruits/1/plant) for IP-IP; and increasing by 0.029 g (0.23 g/1/plant) for IP-1A and 0.024 g (0.19 g/1/plant) for IP-IP in seed productions. The transpiration correlated to oil production but not to oil content. Each mm of transpiration increased the oil production 0.0094 g (0.075 g/1/plant) for IP-1A and 0.0073 g (0.058 g/1/plant) for IP-IP. Water use of IP-IP genotype was more efficient than IP-1A in fruits number, but the IP-1A genotype more efficient in seed and oil productions. The differences on growth and yield responses of the jatropha genotypes to transpiration can be used as the model to describe the relationship between transpiration and growth and yield of the plant.

KEANEKARAGAMAN JA BAMBU DI PULAU SUMBA Kramadibrata, Kartini
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Arbuscular fungi (AF) associated with eight species of bamboo in Sumba island was studied. The study was initiated by collecting soil samples from bamboo rhizospheres followed by mass propagation of AF by culturing in pot. After several months soil from pot cultures were wet sieved and decanted followed by centrifugation and spores of AF were collected, identified by morphological characters under light microscope. The result showed that diversity of AF in bamboo in Sumba island is low but record of Acaulospora foveata on Bambusa blumeana, Dinochloa sp. and Nastus reholttumianus; A. scrobiculata on B. blumeana,Schizostachyum brachycladum (green) and N. reholttumianus; A. tuberculata on Gigantochloa attter, S. brachycladum (yellow) and S. lima; Glomus etunicatum on G. atter; and G. rubiforme on G. atter associated with bamboo is new for Indonesia.

KEANEKARAGAMAN KUMBANG LUCANID {Coleoptera: Lucanidae) DI TAMAN NASIONAL BOGANINANIWARTABONE, SULAWESI UTARA Koneri, Roni
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The objective of the research is to study the lucanids beetle diversity (Coleoptera: Lucanidae) in Bogani Nani Wartabone National Park, North Sulawesi. Research was conducted between June 2008 and August 2008. Ecological samplings were conducted in three degrees of forest habitat (primary forest, secondary forest and margin forest). Beetles were surveyed by using light traps. Results indicates that number of species recorded are 82 individuals consist of 10 species. This results also indicates that habitat type has a strong effect on lucanid beetle community. Statistical analysis shows significant different of species richness, abundance and species diversity among habitat types.

KEANEKARAGAMAN DAN SEBARAN SERANGGA DI KAWASAN PULAU-PULAU KECIL TAMAN NASIONAL KARIMUNJAWA Erniwati, Erniwati
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Study on diversity and distribution of insects was conducted in seven small islands of Karimunjawa National Park. Central Java from March 24 to April 7, 2006. using two collection methods i.e. pitfall traps and sweeping nets. The study collected 108 species of insects from the seven small islands included 9 ordo of 44 families. They were Lepidoptera (27 species), Odonata (7 species).Orthoptera (8 species). Mantodea (2 species). Blattodea (9 species). Hymenoptera (20 species). Diptera (8 species), Coleoptera (12 species) and Hemiptera (15 species). The highest number of species was collected from Karimunjawa Island (96 species), while other islands were low: denting (30 species).Kumbang (23 species).Kembar (26 species), Nyamiik (56 species). Parang (50 species) and Bengkuang (23 species).Base on fuction on nature, these insects could be divided into phytophagous, pollinator, decomposer, pasitoid, vector and unknown.Three species of butterflies were rediscovered during this study - they were Euploea crameri karimondjawensis.Euploea syhesier karimondjawana and Idea leitconoee karimondjawae. while one species Neplis columelle karimondjawae has not been found yet. Shannon. Simpsons, and Fishers Alpha indexes, and similarity of Jackard to compare the difference among the islands is disccussed.

KANDUNGAN KUERSETIN DAN POLA PROTEOMIK VARIETAS JAMBU B ATU (Psidium guajava L.) TUMBUH LIAR DIKAWASAN CIBINONG, BOGOR Jusuf, Eddy
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

This work was purposed to discover the potencies of some guava {Psidium guajava) varieties or cultivars as medicinal and fruit plant, from which the economic value then would be raised. We have collected from about 500 hectares area of our institute including the village surroundings, 35 numbers of morphologically different guavas wild growing consisting of 18 cultivars of red fruit and 17 of white fruit. Methanol extraction of dried leaves giving flavonoids, which by thin layer chromatography Kieselgel 60F2S4, the spots of quercetin of HRf > 50 obtained from all guava varieties. After extraction of each spot and measuring spectrophotometrically, the quercetin content of each plant number was found to be varied in both, among red fruit and white fruit varieties. The highest, >6.0% quercetin content obtained in two numbers of red fruit and one from white fruit and the lowest, < 0.6% obtained in two numbers of white fruit. Proteomic profile from cutted and blended fresh leaves after extraction using anti proteolytic buffer in coldness, bring us to make a phenogram giving the variability of genetic kinship, that 35 varieties were divided in three groups of kinship; first, all cultivars of red fruit, secondly sixteen cultivars of white fruit, and third one cultivar of white fruit.

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