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INDONESIA
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES
Published by Universitas Udayana
ISSN : 20854773     EISSN : 23022906     DOI : -
Core Subject : Health,
Medicine & Pharmacology
Indonesia Journal of Biomedical Science (IJBS), Print-ISSN 2085-4773; E-ISSN 2302-2906 is an international and peer-reviewed journal published twice per year in print and online by Indonesian of Biomedical Association in collaboration to Postgraduate School of Biomedicine Udayana University, Bali-Indonesia which was founded in 2007. The Journal aims to bridge and integrate the intellectual, methodological, and substantive diversity of biomedical scholarship, and to encourage a vigorous dialogue between biomedical scholars and researches. The Journal welcomes contributions which promote the exchange of ideas and rational discourse between practicing educators and biomedical researchers all over the world.
Arjuna Subject : -
Articles 87 Documents
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RESPON KEKEBALAN HUMORAL MENCIT BALB/C YANG DIVAKSINASI DENGAN VAKSIN LIMPA DAN VAKSIN KULTUR PENYAKIT JEMBRANA TERHADAP PROTEIN VIRUS JEMBRANA MANIK WIDIYANTI, NI LUH PUTU; SUATA, I KETUT; MANTIK ASTAWA, I NYOMAN; -, HARTANINGSIH
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 2, No. 2 Mei 2008
Publisher : Udayana University

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Abstract

Sapi bali adalah salah satu aset nasional Indonesia yang harus dilestarikan karena mempunyai keuntungan ekonomi. Tetapi sapi bali mempunyai beberapa kelemahan penyakit khususnya penyakit Jembrana yang disebabkan oleh virus penyakit Jembrana (JDV). Pencegahan terhadap penyakit Jembrana telah dilakukan dengan vaksinasi. Vaksin yang terbukti dapat menurunkan tingkat kematian sapi bali terserang JDV adalah vaksin limpa. Jenis vaksin ini hanya mampu menginduksi kekebalan dengan perlindungan 70%. Proteksi ini dapat ditingkatkan jika jumlah virus yang digunakan dalam vaksin meningkat. Tekhnik kultur in vitro adalah salah satu metode meningkatkan jumlah virus penyakit Jembrana, dan selanjutnya dibuat vaksin kultur. Hasil penelitian menunjukkan bahwa sel limfosit sapi bali terinfeksi JDV adalah 9,5% pada limpa dan 57,43% pada sel kultur. Uji westernimmunoblotting sel limfosit sapi bali dari darah tepi dan limpa terinfeksi JDV menggunakan antibodi monoklonal (AbMo) anti Ca, terdeteksi protein dengan berat molekul 26 kDa, 42 kDa dan 51 kDa. Pada medium kultur PBMC dan endapan plasma sapi bali terinfeksi JDV, teridentifikasi protein dengan berat molekul 16 kDa an 26 kDa menggunakan AbMo, dan teridentifikasi protein dengan berat molekul 16 kDa; 21,5 kDa. 26 kDa; 29,7 kDa; 40 kDa dan 50 kDa menggunakan AbPo. Uji Elisa didapatkan nilai absorban antibodi mencit balb/c yang divaksinasi dengan vaksin kultur penyakit Jembrana lebih tinggi yaitu sebesar 0,3089 dibandingkan vaksin limpa yaitu sebesar 0,177 dengan p<0,05. Nilai absorban antibodi mencit balb/c terhadap antigen Ca, SU dan tat, memperlihatkan nilai absorban terhadap antigen SU berbeda sangat signifikan dibandingkan dengan antigen Ca dan antigen tat (p<0,01). Antigen Ca berbeda signifikan terhadap antigen tat (p<0,05).
INTERLEUKIN-1?, INTERLEUKIN-6, AND ANTAGONIST INTERLEUKIN-1RECEPTOR AS MEMORY IMPAIRMENT RISK FACTOR IN COMPLEX PARTIAL EPILEPSY Purwa-Samatra, D. P. G.; Sudewa-Djelantik, A. A. G.; Suastika, and K.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol 7 No 1 (2013): IJBS Vol.7 No.1 January-June 2013
Publisher : Udayana University

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Abstract

Memory impairment is one of the most common adverse following epilepsy, particularly complex partial epilepsy. Cytokines physiologically play an important role in memory impairment by preventing long term potentiation process in hypocampus. Several literatures have mentioned that IL-1b, IL-6 and antagonist receptor IL-1Ra are crucial cytokines in complex partial epilepsy. This study aims to find out whether high level of IL-1b and IL-6 as well as low level of IL-1Ra might be risk factors of memory impairment in complex partial epilepsy patient. This was a case control study, enrolling 30 complex partial epilepsy patients with memory impairment as case group and 30 complex partial epilepsy patients without memory impairment as control group. In this study, it was obtained that the mean of IL-1? level in case group was significantly higher compared to the control (2.74 ± 4.36 vs. 0.42 ± 0.18 pg/ml, p = 0.007). The mean of IL-6 in case group was significantly higher compare to control (5.89 ± 6.32 vs. 2.34 ± 1.80 pg/ml, p = 0.006). The mean of IL-1Ra level of the case group was not significantly higher compared to the control (519.81 ± 262.64 vs. 413.28 ± 106.85, p = 0.767). By applying bivariate analysis, McNemar’s test, we observed that IL-1? with cut off point 0.63 pg/ml and OR = 70 is a risk factor of memory impairment in complex partial epilepsy indicated by p = 0.001. Similar result was also gained for IL-6 with cut off point 2.87 pg/ml and OR = 4.57 as a risk factor of memory impairment in complex partial epilepsy indicated by p = 0.007. Meanwhile, IL- 1Ra with cut off point 471 pg/ml and OR = 0.727 was not as a risk factor of memory impairment in complex partial epilepsy indicated by p = 0.573. It can be concluded that the high level of IL-1B and IL-6 were the risk factors of memory impairment in complex partial epilepsy patients. High level 1L-1B patient was 70 times higher risk of becoming memory impaired. High IL-6 patients will have the risk nearly 5 times higher. The low level of IL-1Ra does not as a risk factor in epilepsy patients for having the following memory impairment.
LOW PLASMA CORTISOL LEVEL IS A RISK FACTOR FOR ADULT ATOPIC DERMATITIS AND NEGATIVELY CORRELATED TO PLASMA LEVELS OF NOREPINEPHRINE AND INTERLEUKIN-4 Wardhana, Made; Suata, Ketut; Suastika, Ketut
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 4, No. 1 Januari 2010
Publisher : Udayana University

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Abstract

Atopic dermatitis is a skin inflammatory disease characterized by hyperactivity of the humoral immune system with a typical onset in infancy or early childhood. Many studies have focused on the patho-physiological role of the immune system in atopic dermatitis, but since the stress hormone receptor was recognized on the surface of immune cells, it appeared that cortisol was prominent stress hormones in regulating the immune system. Some studies have shown that individuals with atopic diseases such as atopic dermatitis, allergic rhinitis and bronchial asthma had lower concentration of saliva cortisol than those with non-atopic conditions. With this evidence, it can be assumed that lower concentration of cortisol as the result of hypo-response of hypothalamus-pituitary-adrenal to stressor can increase and interleukin (IL-4) concentrations. Both substances stimulate lymphocyte T helper 2 (Th2) cells to synthesize IL-4, which has an important role in atopic dermatitis pathogenesis to increase hypersensitivity to various environmental allergens. Until recently, few studies are available concerning the low concentration of plasma cortisol as a risk factor for atopic dermatitis, as well as its correlation with plasma levels of and IL-4. The purpose of this study was to show that low concentration of plasma cortisol is a risk factor for atopic dermatitis and it is negatively correlated with and plasma IL-4. Matched pair case control design, involving 36 cases and 36 controls, was used to show that low concentration of cortisol is a risk factor for atopic dermatitis, and cross sectional design was applied to find out the negative correlation between cortisol and IL-4 in 88 samples consisting of 52 patients with atopic dermatitis and 36 healthy person or persons without atopy. The result of the case-control study showed that plasma cortisol concentration of the case group was significantly lower (4.89 + 2.1 ug/dl; CI 95 %; p < 0.001) than in those of the control group (9.12 + 2.33 ug/dl) at confidence level of 95%; p < 0.001. Multiple logistic regression analysis showed odd ratio of cortisol 3.45, which was higher than the ratio of other parameters such as IL-4, history of atopy and skin moisture. Plasma and IL-4 concentrations of the patients with atopic dermatitis were significantly higher than in the subjects without atopy. The correlation test indicated that plasma cortisol was negatively correlated to norepinephrine (r = - 0.68; p < 0.05), and IL-4 (r = - 0.55; p = 0.05) levels in patients with atopic dermatitis. Based on the above findings, it can be deduced that low concentration of plasma cortisol, being correlated to increased plasma levels of norepinephrne and interleukin-4, is a risk factor for atopic dermatitis in adult
THE ERGONOMICS PROBLEM IN TROTOIR CONSTRUCTION Sutjana, I Dewa Putu
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 2, No. 1 Maret 2008
Publisher : Udayana University

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Abstract

Trotoir construction has become as one of the priorities of development in every town inBali Province; it is in accordance with the evaluation criteria of successful town development.The functions of trotoir are to provide comfortable and safety path for pedestrians and toprevent the traffic jam. But, unfortunately it has not yet met to the purpose. In relation to theproblem the study had been done to the trotoir condition along the street in Denpasar town. Theresults sowed that in general the trotoir (old and new construction) are not flat, too height, notcontinued, blocked by tree, traffic light pole, or holy building etc. These conditions produceunsafety and uncomfortable for the pedestrians especially to the elderly, handicapped (eye orleg) persons, wheel chair, baby trolley. In some trotoirs the accidents had been happened.Therefore, it is suggested that the trotoir must be constructed flatly, continued, not too height,synchronized with drainage system, street construction, electricity and telephone net work, andtree plantation for the green peace program.
VARIES SHUTTLE RUN EXERCISE METHODS INCREASE AGILITY OF STUDENTS OF SMK NEGERI 2 TONDANO KABUPATEN MINAHASA Sarapung, M; Pangkahila, J. A.; Adiputra, N.; Adiatmika, IPG
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 6, No. 1 Januari 2012
Publisher : Udayana University

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Abstract

ABSTRACT Achievment of Indonesian athletes at international level are only resting on badminton, archery, and weight lifting. Meanwhile, other sports do not exhibit encouraging achievement. Agility is one of the important factor on sports including group and individual sports. This is an experimental research with randomized pretest posttest conrol group design. Sample are 75 students recruited from SMK Negeri 2 Tondano Kabupaten Minahasa. Sample was grouped into 5 groups, each group composed of 15 students. The five groups observed were group 1 with treatment of 4 x 30 m shuttle run, group 2 treated with 8 x 15 m shuttle run, group 3 with 12 x 10 m shuttle run, group 4 within 16 x 7.5 m shuttle run, and the last is control group. Exercise was carried out for 6 weeks with a frequent of 3 time per week. Agility pre and posttest data were analysed using anova oneway followed by LSD Post Hoct Test at 5% significant level. This study obtained that there were a significant different among the five groups observed. Shuttle run exercise method with dose of 16x7.5 m increase the highest agility.
IMPROVEMENT OF WORK QUALITY BY APPLYING ERGONOMIC APPROACH INCREASES PERFORMANCE OF TRADITIONAL PORTERS IN BADUNG MARKET DENPASAR Hutagalung, Robert; Manuaba, A; Nala, I Gusti Ngurah; Sutjana, I Dewa Putu
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 3, No. 2 Juli 2009
Publisher : Udayana University

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Abstract

Female traditional porters work every evening at Badung market from 7 pm to3.30 am. Age interval of the porters is 18 – 40 years. The weight of goods carried by asingle porter is 60 – 100 kilograms plus 1-2 kilograms of the basket’s weight, carriedon the head. The distance of each porting activity is 100 meters. From ergonomicconcept, the burden is excessive and may cause injuries such as damage ofintervertebral discs, pain, excessive fatigue and head and neck muscles disorder. Thesymptoms are more obviously seen in those over 40 years old for most of them are notvery capable of working, even some of them need medical treatment. To overcome thisnon-ergonomic work condition, a work quality improvement was carried out to 11sampled porters, including work position, method, carrying weight and equipmentdesign in order to improve the unnatural work position and to give chance to them tohave an active rest as well as to alter the static work system to be more dynamic.Results of this study were: (1) average of pressure force on L5/S1 before improvementwas 7,967.65 ± 66.78 N and after improvement was 2,983.26 ± 16.63 N; (2) average ofmusculoskeletal complaints before improvement was 61.07 ± 0.72 and afterimprovement was 42.76 ± 1.21; (3) fatigue average before improvement was 77.44 ±3.93 and after improvement was 50.36 ± 2.21; (4) pulse rate average beforeimprovement was 150.61 ± 1.06 pulses/minute and after improvement was 119.51 ±1.39 pulses/minute; and (5) average productivity before improvement was 1.78 x 10-2 ±0.01 x 10-2 and after improvement was 2.24 x 10-2 ± 0.03 x 10-2. The results analysisshowed that improvement of work quality by applying ergonomic approach coulddecrease the pressure force on L5/S1 of 60.94 % (p<0.05), musculoskeletal complaintsof 29.99 % (p<0.05), level of fatigue by 34.97 % (p<0.05) and work load by 42.59 %(p<0.05), as well as could increase the work productivity to 26.04 % (p<0.05).Therefore, it is conclused that the improvement of work quality by applying ergonomicapproach increases performance of traditional porters.
HUBUNGAN POSITIF ANTARA ULKUS KAKI DIABETIK DENGAN PERSENTASE SEL BERMARKAH CD4+ PEMBAWA MALONDIALDEHID Putu SutirtaYasa, I Wayan; Suastika, Ketut; Sudewa Djelantik, Anak AgungGede; Mantik Astawa, I Nyoman
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 3, No. 1 Januari 2009
Publisher : Udayana University

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Abstract

Tingginya angka kejadian ulkus kaki diabetik (UKD) dan luka di kaki yangsulit sembuh memberi petunjuk kemungkinan ada proses kematian sel imun yangsangat banyak dan belum jelas mekanismenya secara molekuler pada jaringan UKD.Telah diteliti hubungan antara derajat UKD dengan persentase sel bermarkahCD4+ pembawa malondialdehid (MDA). Penelitian ini adalah penelitianobservasional dengan rancangan cross sectional analytic study yang dilakukan diRumah Sakit pemerintah dan swasta di Denpasar, Badung, Tabanan, dan Gianyar.Parameter yang diukur dari bahan darah adalah kadar gula darah memakai metodeenzimatik (heksokinase), dan dari bahan jaringan kaki, dihitung sel bermarkah CD4+pembawa MDA memakai metode imunohistokimia (reagen dari Biodesign danAbcam ). Dari 80 sampel UKD didapatkan 49 (61,2%) penderita laki-laki dan 31(38.8%) penderita wanita, berdasarkan tingkat keparahan UKD, sampel dipilah lagimenjadi: 29 (31,9%) derajat 2; 20 (21,9%) derajat 3; 13 (14,3%) derjat 4; dan18(19,8%) derajat 5, rata-rata persentase sel bermarkah CD4+MDA adalah 75,0 ±20,5 %, Didapatkan korelasi positif kuat antara persentase sel bermarkah CD4+pembawa malondialdehid dengan derajat UKD (r = 0,71; p < 0,01). Pada penelitianini membuktikan ada mekanisme kematian sel imun dan sekaligus menjawabpermasalahan bahwa pada penderita UKD mudah terkena infeksi dan sulit untukdisembuhkan, dengan dibuktikan bahwa ada korelasi positif kuat antara derajat UKDdengan persentase pembentukan MDA dari sel bermarkah CD4+, ini menyatakanbahwa semakin berat derajat UKD semakin banyak mengalami kematian sel imun..
GARCINIA MANGOSTANA L. RIND EXTRACT AND PHYSICAL TRAINING REDUCE OXIDATIVE STRESS IN WISTAR RATS DURING MAXIMAL PHYSICAL ACTIVITY Arsana, I N.; Adiputra, N.; Pangkahila, J. A.; Putra-Manuaba, and I. B.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol 7 No 2 (2013): IJBS Vol2 No2
Publisher : Udayana University

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Abstract

Oxidative stress is a condition of imbalance between the production of free radicals or reactive oxygen species (ROS) and antioxidants, in which the levels of free radicals higher than antioxidants. One causes of oxidative stress is the maximum physical activity. Oxidative stress can be reduced by antioxidants. One source of antioxidants is mangosteen rind (Garcinia mangostana L). This study aimed to investigate the role of mangosteen rind extract and physical training in reducing Malondialdehyde (MDA, increasing Superoxide dismutase (SOD) and Glutathione Peroxidase (GPx). This study used a randomized block design with factorial pattern of 6 x 2 with four repetitions. The first treatments are mangosteen rind extract with doses; 0, 50, 100; 200; 300, and 400 mg/kg bodyweight/day for four weeks. The second treatments are the physical training consists of; without and with physical training. MDA, SOD and GPx were assessed in the end of treatment. Data were analyzed with GLZ. The results showed that average of MDA, SOD and GPx different significantly (p<0.05) after administration of extracts as well as physical training. Extracts and physical training concurrently also showed effect significant (p<0.05). However, at doses 0 until 300 mg/kg bodyweight, MDA levels more higher while SOD and GPx lower significantly (p<0.05) at training group compared with without training. Whereas at dose 400 mg/kg bw, MDA lower at training (p>0.05), while SOD and GPx were recorded significantly (p<0.05) higher than the group without training. Overall, it could be concluded that physical training with administration of mangosteen rind extract reduce oxidative stress through reduction of MDA, as well as increased both SOD and GPx.
ANTIOXIDANT CAPACITY OF FRANGIPANI (Plumeria alba) POWDER EXTRACT Wrasiati, Luh Putu; Wirawan, I Gede Putu; Agus Bagiada, Nyoman; Mantik Astawa, I Nyoman
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 5, No. 2 Mei 2011
Publisher : Udayana University

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Abstract

This research aimed to identify the antioxidant capacity, vitamin C (ascorbic acid), and total phenolic compounds of frangipani flower powder. The powder was extracted using ethanol, methanol, acetic acid, and water (aquadest). Antioxidant capacity of each extract were determined using 2,2-diphenyl-1-picrylhydrazyl radical scavenging method, vitamin C were determined using 2,4 Dinitrophenylhydrazine, and total phenolic compounds were determined using Folin Ciocalteu reagent. All of parameters were measured by spectrofotometer. The result shows that the highest value of antioxidant capacity was ethanolic extract (18.19%) and the lowest value was acetic acid extract (12.74%). The highest value of vitamin C was aqueous extract (3.49 mg/100g) and the lowest value was acetic acid extract (3.02 mg/100g). The highest value of total phenolic content was aqueous extract (25.49 mg GAE/g) and the lowest value was acetic acid extract (22.74 mg GAE/g). In conclusion, the higher antioxidant capacity was not always followed by the higher of vitamin C and total phenolic compounds.
CANTHARANTHUS ROSEUS LEAF EXTRACT ACCELERATES HEALING OF WOUND WISTAR RAT Anom-Dada, I K.; Berata, I K.; Putra-Manuaba, I. B.; Damriyasa, and I M.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol 7 No 1 (2013): IJBS Vol.7 No.1 January-June 2013
Publisher : Udayana University

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Abstract

Cantharantus roseus (C. roseus) is a medical plant that has been used to treat various diseases. This study was carried to determine C. roseus leaf extract able to acclerate wound healing in Wistar rat. Full skin-tickness round wounds were created on the back of the rats. Wound healing was evaluated after treated with 15% of a mixture of leaf extract of C. roseus and vaseline. The rats were divided into two groups, each group consist of 16 rats. Wound healing was assesed in five and fifteen days after treatment by measuring wound area, wound closure, and counting the new capillary vessel number (angiogenesis). The result of the study indicated that extract of C. roseus leaf accelerates the wound healing in Wistar rat. Coverage rate of wound is faster on treated group than control group (p<0.05). Wound closure and angiogenesis in early wound healing were higher in treated group than control group (p<0.05). These results suggested that the first phase of wound healing was shortened, and the proliferative and maturation phases were advanced by methanol extract of C. roseus leaf. Therefore, it can be concluded that C. roseus could be potential to help in topical management of wound healing.  

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