cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota denpasar,
Bali
INDONESIA
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES
Published by Universitas Udayana
ISSN : 20854773     EISSN : 23022906     DOI : -
Core Subject : Health,
Medicine & Pharmacology
Indonesia Journal of Biomedical Science (IJBS), Print-ISSN 2085-4773; E-ISSN 2302-2906 is an international and peer-reviewed journal published twice per year in print and online by Indonesian of Biomedical Association in collaboration to Postgraduate School of Biomedicine Udayana University, Bali-Indonesia which was founded in 2007. The Journal aims to bridge and integrate the intellectual, methodological, and substantive diversity of biomedical scholarship, and to encourage a vigorous dialogue between biomedical scholars and researches. The Journal welcomes contributions which promote the exchange of ideas and rational discourse between practicing educators and biomedical researchers all over the world.
Arjuna Subject : -
Articles 87 Documents
 2   3   4   5   6 
ALDEHYDE DEHIDROGENASE LEVEL AND FATTY ACID ETHYL ESTER AS BIOCHEMICAL MARKERS PERSIST LONGER THAN ETHANOL IN WISTAR RATS AFTER CHRONIC ALCOHOL CONSUMPTION Suaniti, Ni Made; Sudewa Djelantik, A. A. Gde; Suastika, Ketut; Mantik Astawa, Nyoman
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 4, No. 2 Juli 2010
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.344 KB)

Abstract

Alcohol consumption in human has increased from year to year in Indonesia and more recently, anincreasing number of cases of alcohol intoxication, alcoholic liver disease, and death were observed.The purpose of this experimental study was to examine the significance of two known biochemicalmarkers of alcohol given by mouth in the Wistar rats. The study design used was the “Truerandomized experimental post test only control group design". The rats were randomly distributedaccording to the experimental design and were treated daily for six weeks (chronic intake) with 5%and 20% alcohol. This study used 15 rats with 5 rats for treatment group treated with 5% alcohol, 5rats for treatment group treated with 20% alcohol, and 5 rats as control group treated with distilledwater. The biochemical markers were aldehyde dehydrogenase (ALDH) and Fatty Acid Ethyl Esters(FAEE). ALDH and FAEE were two biochemical markers of ethanol which are sensitive and specificfor alcohol consumption. The study was conducted in two phases. Initially, rats were treated orallyeveryday for six weeks with 5% and 20% alcohol, and then the blood level of ethanol, ALDH andFAEE were measured. Blood samples were collected at 6 and 24 hours after the last oral intake ofchronic alcohol administration. Qualitative analysis was carried out to detect the presence of ethanol,ALDH, and FAEE in the treatment groups and quantitative analysis to determine their levels in theblood of Wistar rats. Statistical analysis of ALDH was done by using parametric test and the presenceof FAEE persisting longer than ethanol by non-parametric test. The results showed that ALDHpersisted and increased significantly following chronic consumption of alcohol in the rats. Similarly,FAEEs persisted longer than ethanol after alcohol intake. After six hours, the ALDH level increasedby 108.14% in the rat treated chronically with 5% alcohol and by 85.07% in rat treated with 20%alcohol. After 24 hours, FAEE also persisted longer in the blood than ethanol following treatmentwith alcohol 5%. ALDH levels increased by 83.11% after chronic treatment with 5% alcohol and by112.05% in the rats treated with 20% alcohol. In the blood collected 24 hours after the last treatmentwith 5% alcohol, ALDH increased by 95.11% and by 86.79% in the rats treated with 20% alcohol.FAEE persisted longer than ethanol in the blood following administration of 5 % and 20% alcoholboth at 24 hours following chronic treatment. The longer persisting ALDH and FAEE were new andgood biochemical blood markers for chronic alcohol consumption in the Wistar rats.
BALINESE AGRICULTURE IN A CHANGING WORLD Sutjana, I.D.P.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 2, No. 1 Maret 2008
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1243.489 KB)

Abstract

Agriculture has been and continues to be one of the economic foundations of Bali and to be a major livelihood for Balinese people. The wide of agricultural area or rice field to be a symbol of community prosperity. It is accepted that in the region which consists of wide of rice fields is considered as a rich region and their inhabitants consequently have a better standard of living, due to having enough food. Because the agricultural sector capable to enhance the community prosperity the people should give effort to own more rice field (build or bought). All of agricultural activities influenced the daily community activity and become community cultural as well as organization, work tool, household tool, value system, plant which are cultivated, ceremony etc. always related to the agriculture. Agricultural technology (machine or method) used by the Balinese farmers still simple and inherited from generation to generation. It seems to be no development or progress resulted in that
DOSE AND SAFETY OF HYPOETES POLYTHYRSA MIQ ETHANOL EXTRACT FOR DISSOLVING RENAL CALCULI: an affordable medication K, Widana; I G. M., Adioka; D. K., Ernawati
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol 6 No 2 (2012): Indonesian Journal of Biomedical Sciences
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.114 KB)

Abstract

Renal calculi develops when the urine contains more crystal-forming substances, such as calcium, uric acid and a compound called oxalate. Most renal calculis are calcium-based, usually in combination with oxalate. Hypoetes Polythyrsa Miq has been used empirically to prevent or treat renal calculi. This study aims to identify toxicity or side effect of Hypoetes Polythyrsa Miq ethanol extract for dissolving renal calculi, therefore this extract can be applied safely and effective for curing renal calculi. An in vitro research with pre-post control group design was conducted at Pharmacy Department Faculty of Medicine Udayana University. In addition, to determine as to whether the extract is safe orally, LD50 test was also conducted using mice strain BALB/c. The results showed that ethanol extract of Hypoetes Polythyrsa Miq leaves has dissolved renal calculi significantly (? <0.05).  Five percent of the extract was effectively dissolving calcium stone 3.36 times higher than control positive (BE). It was also proven that an acute LD50 was achieved at a dose of 0.694 g extract (139% of usual dose). It can be concluded that the use of ethanol extract  per oral in short term therapy is safe. Keywords: traditional medicine, hypoetes polythyrsa Miq, renal calculi.
Ergonomic Intervention on the Stamping Process of Part Body Component Improved Work Quality and Satisfaction and Time Efficiency at the Stamping Plant Division of PT ADM Jakarta Isna Oesman, Titin; Manuaba, A.; Adiputra, N.; Sutjana, D.P.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 4, No. 1 Januari 2010
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.653 KB)

Abstract

Part body component of a car is the finished product component that is mainlyproduced by the big press machine. In the production process of part body component of acar in the stamping plant division, the manual task is performed by two operators to feedingthe material sheet into the big press machine simultaneously. After the knobs were pressedtogether, the big press machine started to stamp the material sheet and the outcoming productis taken by the next operator. This task is performed repeatedly until it is the last one.Manual and comprehensive task that combined with speed in operating the big press machinecause who muscular tension which at the end could cause muscular complaints and prematurefatigue, that could decrease production and productivity.The current study of ergonomic intervention on stamping process of part bodycomponent of car was conducted in order to determine whether ergonomic intervention couldimprove the work quality, satisfaction and time efficiency in the stamping process.The subjects of study were 10 persons that were taken randomly. It was designedexperimentally with treatment by subject design. The method of data collection was byquestionnaire distribution and direct measurement of subject’s condition, time of stampingprocess and work environment before and after intervention. The Shapiro Wilk test showedthat all of the data had normal distribution and was followed by t-paired for data of workquality, work satisfaction, time efficiency, and the environment data. The significance level isset at 5%.The research result showed that work quality was improved (muscular complaints wasdecreased by 6,65%, fatigue was decreased by 5,47% and work boredom was decreased by5,87%), work satisfaction was improved up to 6,43%, time efficiency of stamping processwas improved up to 10,7%, the production was improved up to 2,59% and productivity wasimproved up to 32,65% as well as company’s profit was increased up to 3,95%.Hence, the study concludes that ergonomic intervention in the production process ofpart body component of car in the stamping plant division could improve work quality, worksatisfaction and time efficiency.
EFFECT OF ORAL IRON TABLET ADMINISTRATION ON SERUM FERITIN AND HEMOGLOBIN CONCENTRATION OF PRE-PREGNANT WOMEN WITH MILD IRON DEFICIENCY ANEMIA IN BALI Seri Ani, Luh; Bakta, I Made; Suryadhi, INT; Agus Bagiada, IN
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 1, No. 3 Desember 2007
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (61.037 KB)

Abstract

Iron deficiency anemia (IDA) is still to be a problem of pregnant women healthrelated to its high prevalence and its negative effects on health. Prevention efforts by ironsuplementation in pregnant woman have not reducing IDA problem in pregnant women yet. This failure is probably due to the assumption that IDA have been seen pre-pregnant. Totest this hypothesis, a quasi experimental study was conducted by randomized pre and posttest control group design. Sample were collected by multistage sampling random technicconsist of 47 women in treated group and 52 in control group. Both group were serumferritin and hemoglobin value test untill 3 time, pre-pregnant, early pregnant and duringpregnant. Iron tablet was administrated to treated group from the beginning of pre-pregnantperiod, continued until the first 3 months of pregnancy, while in control group iron tabletwas only given during the first 3 months of pregnancy. T-group result shown that meanserum ferritin and hemoglobin concentration at pregnant women on treated group(33,45±14,12 ?g/dL dan 12,25±1,20 g/dl) more high than control group (19,65±8,99 ?g/dLdan 10,91±0,67 g/dl), p<0,05. Mean difference serum ferritin and hemoglobinconcentration at pregnant women is 13,8 ?g/dL dan 1,34 g/dl (p<0,05). Benefid analysisresult shown that iron suplementation since pre-pregnant more benefid than ironsuplementation during pregnant (BCR >1). Based on these results, it can be concluded thatiron supplementation to IDA women starting from pre-pregnant period results in a bettereffect compare to oral iron supplementation during pregnancy only.
The Lactate/Pyruvate Ratio of Metabolic Modulation using Glucose Insulin Kalium and Lactate Solution and their Effect on Functional Mechanical Recovery of the Isolated Perfused Heart Suranadi, I Wayan; Bakta, Made; Arhya, I Nyoman; Leverve, Xavier
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 3, No. 1 Januari 2009
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (65.787 KB)

Abstract

Metabolic modulation with Glucose Insulin Kalium (GIK) solution has beenreally well known in their capacity to improve post ischemic heart function. In this regardGIK intervention on post operative Coronary Artery Bypass Graft (CABG) can improveheart function recovery on reperfusion period (Goldhaber dan Weiss, 1992; Atwell et al.,1997). Post operative CABG intervention with GIK will produce a beneficial effect onthe elevation of heart energy to prevent ionic homeostasis disturbance and reactiveoxygen species (ROS) production that become the basis of reperfusion injury (Silvermandan Stern, 1994; Cross et al., 1995; Taegtmeyer et al., 1997; Opie, 1999; Lazar, 2002;Doenst et al., 2003; Trence et al., 2003).Many efforts have been made to clarify how exactly GIK works to improve postischemic heart function as in CABG. This is crucially done in order to be able to modifythe solution concerned. Although this solution has been clearly proved to improve postischemic heart function, it is not totally free from its adverse effect. Its main side effect isthat it can provoke hyperglycemic state, which contrasts with the tight glucose control incontinuously normal range for the patients who are critically ill.In this study lactate and pyruvate level in the coronary effluent were measuredfrom the isolated heart directly perfused with GIK and lactate. It was shown that thepreischemic lactate level was low and then clearly elevated as soon as the reperfusiontook place due to anaerobic metabolism. In accordance with reperfusion time lactate leveldecreased gradually. In relation with pyruvate level, this substrate evolution looked likethe appearance of lactate but its value was lower if compared with lactate.The recovery in functional mechanical activity of the post ischemic heart seems tobe much more related to the pattern of the evolution of logarithmic lactate/pyruvate ratio(L/P ratio). Logarithmic value of L/P ratio in GIK group increased since the earlyreperfusion period (+40%, p < 0.05), followed by improvement in recovery ofmechanical activity in this group which was significantly higher if compared with thecontrol group. Similar fashion was found in lactate group in regard to the evolution of thelogarithmic value of L/P ratio in this group, where its value was significantly highercompared with the control group. The logarithmic evolution pattern on L/P ratio for thisgroup increased along the reperfusion time (+34% p < 0.05).From the present study, it can be concluded that the recovery of functionalmechanical activity of the post ischemic heart perfused with GIK is through modificationon cellular lactate metabolism.
IN VITRO INHIBITION ZONE TEST OF BINAHONG (ANREDERA CORDIFOLIA) TOWARDS STAPHYLOCOCCUS AUREUS, ENTEROCOCCUS FAECALIS, ESCHERICHIA COLI, AND PSEUDOMONAS AERUGINOSA Amertha, IBPM; Soeliongan, S; Kountul, C
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 6, No. 1 Januari 2012
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.245 KB)

Abstract

ABSTRACT This is a true experimental research with post test-only control group design. The study was conducted to test the inhibitory zone of the Binahong leaf extract (Anredera cordifolia) against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa. Binahong leaf extract is prepared using maceration technique, by soaking it in a sealed jar for 24 hours with 95% methanol. Then subsequently filtered using a funnel with filter paper, and the filtrate is collected inside an erlenmeyer. The filtrate then concentrated using a rotavapor, this concentrated extract dissolved into aquadest with a concentration of 50 ppm, 100 ppm and 1000 ppm. By taking a few colonies with a sterile loop into a stock of Staphylococcus aureus, Enterococcus faecalis, Esherichia coli, Pseudomonas aeruginosa then scratch it into MH blood agar medium, and incubate it for 24 hours with a temperature of 370C. The next day, bacterial suspension was made in test tube, which already contains 0.9% NaCl. The suspension tturbidity is equivalent to 0.5 Mc Farland. Bacterial inhibition zone of binahong leaf extract (Anredera cordifolia) is tested using absorbance disc method or better known as the Kirby-Bauer method. First, pour 10 ml of agar medium (± 400C) into a cup (petridish) and then wait until it’s cold. After the medium becomes solid, the suspension of bacteria Staphylococcus aureus, Enterococcus faecalis, Esherichia coli, and Pseudomonas aeruginosa are slowly smeared with sterile cotton sticks on the surface of the media. Soak the paper discs into binahong leaf extract (Anredera cordifolia) with concentrations of 50, 100, and 1000 ppm, for about 5 minutes, and placed it on the surface of the petridish, together with the positive control (amoxicillin) and negative control (aquadest). Then incubate it at 370C for 24 hours. The effectiveness of binahong leaf extract (Anredera cordifolia) inhibition zone, can be determined by measuring the diameter of clear zone around the paper using a sliding-term. Binahong leaf extract (Anredera cordifolia) zone of inhibition is negative, a very slight different is showed by the amoxicillin inhibition zone, for having a clear zone diameter of 28 mm for Staphylococcus aureus and Esherichia coli, and 21 mm for Enterococcus faecalis. This fact is probably caused by several things concerning the mechanism of action of a substance as an anti bacterial of the binahong leaf extract (Anredera cordifolia).
THE HIGH PLASMA RETINOL BINDING PROTEIN 4 LEVEL AS A RISK FACTOR CONSEQUENTLY OF TYPE 2 DIABETES MELLITUS OF ABDOMINAL OBESITY Budhitresna, AAG; Suastika, Ketut; Mantik Astawa, Nyoman; Santoso, Anwar
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 5, No. 1 Januari 2011
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.463 KB)

Abstract

Abdominal obesity (Ab-Ob) related to cardiometabolic risk, that is riskfactor constellation for succeeded cardiovasculer disease and type 2 DiabetesMellitus (DM). That factors such as atherogenic dislipidemia, hypertension,hyperglycemia, protrombotic state, and proinflammation state. Type 2 DMcharacterised by insulin resistance (IR). Plasma levels of retinol binding protein 4(RBP4) that is secreted by adipocytes are increased in insulin resistance (IR) state.Experiment in mice suggest that elevated RBP4 level cause IR. Although theunderlying mechanism is not clearly understood, RBP4 considered playimportance role consequently of type 2 DM in Ab-Ob.This research was carried out to determine the role of high plasma RBP4level as a risk factor consequently of type 2 DM in Ab-Ob. The research wasconducted by cross sectional analytic in 81 patients with Ab-Ob and case controlstudy with matching on 33 patients with Ab-Ob type 2 DM as cases and 33patients with Ab-Ob non type 2 DM as control. The plasma of TNF-?, sTNFR1,and RBP4 levels was measured by ELISA. IR status of the patients wasdetermined by HOMA-IR, whereas the ?-cell function was determined byHOMA-B. Ab-Ob was defined by using criteria for Asian peoples (male WC ? 90cm; female WC ? 80 cm). The result of 81 patients with Ab-Ob showed that bothplasma of TNF-? and sTNFR1 levels were significant positive correlated withplasma RBP4 level (coeficient correlation r = 0,294; p = 0,008 dan r = 0,458; p =<0,001 respectively). In addition, the plasma of RBP4 level significantly positivecorrelation with HOMA-IR (r = 0,450; p = 0,000) and significantly negativecorrelation with HOMA-B (r = -0,564; p = <0,001). In the matched case-controlstudy, it was shown that mean plasma of RBP4 level of type 2 DM group (76,08 ±16,84 ?g/ml) statistically higher than that without type 2 DM group (41,13 ±14,75 ?g/ml) (p = <0,001). The odds ratio higher plasma of RBP4 level was 5,426(CI 95%; 1,343 – 21, 928) statistically significant for increases risk type 2 DM (p= < 0,05). It has been proven that RBP4 was a dominant and consisten risk factor(66.9%, p = < 0.001) which influenced the incidence of type 2 DM in Ab-Ob. It can be concluded that high plasma of RBP4 level have a greater risk tosuffered from type 2 DM compared to low plasma of RBP4 in Ab-Ob. The highplasma of RBP4 level is most dominant and consistent risk factor consequently oftype 2 DM. These mechanism could behind the association between high plasmaof RBP4 level and type 2 DM.
FRAKSI HEKSAN EKSTRAK BIJI PEPAYA MUDA DAPAT MENGHAMBAT PROSES SPERMATOGENESIS MENCIT JANTAN LEBIH BESAR DARIPADA FRAKSI METANOL EKSTRAK BIJI PAPAYA MUDA Satriyasa, Bagus Komang
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 2, No. 2 Mei 2008
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.165 KB)

Abstract

Fraksi ekstrak heksan mengandung dua golongan zat aktif yang bersifat antifertilitas yaitu golongan steroid dan golongan triterpenoid yang diperkirakan bersifat antifertilitas, walupun mekanisme kerjanya belum jelas. Rancangan penelitian yang digunakan ialah ‘’ Pre-test post-test control group design’’. Penelitian ini memakai 30 ekor mencit jantan strain balb C , umur sekitar 12 minggu dengan berat 20-22 gram, kemudian dikelompokkan secara random menjadi 3 kelompok yang masing-masing terdiri dari 10 ekor. Satu kelompok kontrol (P0 = yang diberikan aquabides), dan dua kelompok perlakuan (P1 = kelompok perlakuan yang diberikan fraksi heksan ekstrak 20 mg/20 gram/hari, P2 = kelompok perlakuan yang diberikan fraksi ekstrak metanol 20 mg/20 gram/hari). Setelah 36 hari perlakuan lalu dilakukan pemeriksaan testis dan darah mencit. Data yang diperoleh dianalisis secara statistik dengan menggunakan uji normalitas Kolmogorov Smirnov Goodnees of Fit test, uji homogenitas, dan uji anova. Didapatkan hasil bahwa fraksi heksan ekstrak maupun metanol dapat menurunkan jumlah sel spermatogonia A, sel spermatosit primer pakhiten, sel spermatid, dan sel Sertoli secara sangat bermakna (p < 0,01), sedangkan jumlah sel Leydig dan kadar hormon testosteron menurun tidak bermakna (p > 0,05). Dari hasil penelitian ini dapat disimpulkan bahwa fraksi heksan ekstrak biji pepaya dapat menurunkan jumlah rata-rata sel spermatogonia A, spermatosit primer pakiten, spermatid, sel Sertoli, sel Leydig dan kadar hormon testosteron lebih besar dari pada fraksi metanol ekstrak biji pepaya muda.
A NOVEL APPROACH OF VIROTHERAPY BASED HSF-1 shRNA IN CANCER ERADICATION Supadmanaba, I G. P.; Wibawa-Manuaba, I. B. T.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol 7 No 1 (2013): IJBS Vol.7 No.1 January-June 2013
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (817.183 KB)

Abstract

Cancer is the second leading cause of death worldwide with continues rise mortality rate. Current cancer treatment modalities are still ineffective and associated with many side effects leading to robust research to find new specific target therapy. Heat shock factor (HSF)-1 is heat shock response mediator protein and act as transcription factor for HSP encoding gene. Many cancers have up-regulated HSP as a result of increase HSF-1 expression. Interestingly, inhibition of HSF-1 has no effect to normal cell, indicating HSF-1 as promises target therapy. RNAi is potential mechanism to block and down regulate HSF-1 which will affect many cellular processes in cancer cell. Combining RNAi base treatment with oncolityc viruses will boost the therapeutic effect of this novel treatment. Despite its potency, this modality still need further research in order to evaluate its efficacy and optimal doses to gain optimal result.

Page 4 of 9 | Total Record : 87

 2   3   4   5   6 

Filter by Year

2007 2014


Reset
Filter By Issues
All Issue Vol 8 No 1 (2014): IJBS Vol8 No1 Vol 7 No 1 (2013): IJBS Vol.7 No.1 January-June 2013 Vol 7 No 2 (2013): IJBS Vol2 No2 Vol 6 No 2 (2012): Indonesian Journal of Biomedical Sciences Vol. 6, No. 1 Januari 2012 Vol. 5, No. 2 Mei 2011 Vol. 5, No. 1 Januari 2011 Vol. 4, No. 2 Juli 2010 Vol. 4, No. 1 Januari 2010 Vol. 3, No. 2 Juli 2009 Vol. 3, No. 1 Januari 2009 Vol. 2, No. 2 Mei 2008 Vol. 2, No. 1 Maret 2008 Vol. 1, No. 3 Desember 2007 More Issue