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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 19 Documents
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Search results for , issue "Vol 14 No 2 (2013)" : 19 Documents clear
Ekstrak Pegagan Meningkatkan Titer Antibodi Mencit Setelah Diinfeksi Salmonella typhi (CENTELLA ASIATICA EXTRACT INCREASE ANTIBODY TITER IN MICE AFTER SALMONELLA TYPHI INFECTION) I Nengah Kerta Besung; I Nyoman Mantik Astawa; I Ketut Suatha; Hartaningsih .
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was conducted to find out the ability of Centella asiatica (C. asiatica) in enhancing antibodyresponse of C. asiatica treated mice following Salmonella typhi (S. typhi) infections. It is therefore expectedthat herbal drug such as  C. asiatica  can be used as an alternative medicine to prevent and to curesalmonellosis both in animals and human. Experimental laboratory studies were conducted usingCompletely Factorial Randomized Design. Mice were divided into four groups and they were treatedrespectively with destilated water (negative control), 125, 250, and 500 mg/kg BW/day of  C. asiaticaextract. The treatment was conducted daily for two weeks  and the mice were inoculated with 105 cells/mlof  S. typhi. The antibody response were examined by indirect enzyme-linked immunosorbent assay (ELISA)on first day, second week and fourth week  after S. typhi infections.  The result showed that treatment ofmice with C. asiatica extract significantly (p<0,05) enhanced antibody titer of Balb/c mice after S. typhiinfections. The highest antibody titer was observed at four weeks after S. typhi infections with 500 mg/kgBW/day (94,0370 ± 1,69 IU).
Kepadatan Sel Hipokampus Insulin Imunoreaktif pada Formasi Hipokampus Mencit yang Diinduksi Berulang dengan Streptozotosin (THE DENSITY OF HIPPOCAMPUS INSULIN IMMUNOREACTIVE CELLS IN HIPPOCAMPUS FORMATION OF REPEAT STREPTOZOSIN INDUCED MICE) Erwin .; Tri Wahyu Pangestiningsih; Sitarina Widyarini
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The presence of insulin in the hippocampus may indicate its involvement in brain cognitive function,such as learning and memory phenomena. The purpose of this study was to find out the density ofhippocampus insulin immunoreactive cells in hippocampus formation in Balb-C mice which treated withstreptozosin repeated as the animal model of diabetes mellitus. Thirty male mice Balb-C strain, aged 12-14 weeks, weight 30-40 g, divided into 2 treatment groups, each group consisted of 15 individuals. GroupI (KI) was treated with sodium citrate buffer, while group II (K2) was treated with streptozotosin at  dose0,5 ml of 40 mg/kg bw in 50 mM sodium citrate buffer pH 4.5 in intra-peritoneal of for five consecutive days.Every two animals from each group euthanasia and necropsied on day 7, 14, 21 and 28 respectively afterthe administration of treatment. Subsequently, the brain tissues were collected and fixatived in NBF10%. Brain sampel were the processed immunohistochemically using anti-insulin mouse antibody. Thedensity of hippocampus insulin immunoreactive cells in hippocampus formation in group 1 were highercompared to group 2. This comparasion as well as the time of observation and interaction between groupand time showed significant differences (p<0.05). it can be concluded that low-dose induction of repeatedstreptozotosin may  cause a decrease in density of hippocampus insulin imunoreaktif cell.
Faktor-Faktor Risiko Tertular Flu Burung di Desa-Desa Kabupaten Klungkung, Bali (THE RISK FACTOR OF BIRD FLU CASES IN VILLAGES IN KLUNGKUNG REGENCY, BALI) I Gusti Ngurah Badiwangsa Temaja; I Nyoman Suartha; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was carried  out to identify the risk factor of  bird flu cases in villages in Klungkung regency,Bali. The data were collected through observation and interviews using questionnaire to head of householdsin villages of which were officially recorded as infected and uninfected area of bird flu.  Data was analyzedusing chi-square test and odds ratio to see the relationship AI infection and risks factors, as well as howsignificance is the relationship between them. The study showed that the crucial risk factor to bird flucases in infected villages were ceremonies (odds ratio : 2.401) and traditional rituals (odds ratio 3.229).The occurrence to bird flu in both villages is significantly affected by the following fctors: (1) poultry forconsuming, ceremony, and traditional ritual from markets and collected poultry, (2) disposal of deadchicken in to the  river, (3) the operation of villages markets, (4) Trading of live  poultry, (5) Commercialpoultry livestock in respected villages, (6) herding duck after rice harvesting, (7) frequency  of traditionalceremonies  in the villages.
Kandungan Senyawa Aktif dan Daya Antibakteri Daun Sambung Darah (ACTIVE COMPOUNDS AND ANTIBACTERIAL ACTIVITY OF EXCOECARIA AGALLOCHA) Masniari Poeloengan; Andriani .
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Sambung darah (Excoecaria agallocha L.) leaves are often used as traditional medicine. This experimentwas done to analyze the compounds of E. agallocha extract against  antibacterial activity either on Grampositive bacteria (Staphylococcus aureus, S.  epidermidis, and Streptococcus agalactiae), and Gram negativebacteria  (Escherichia coli and Salmonella sp.). Leaves extract of E. agallocha L. Was diluted with or madewith methanol percolation. Antibacterial activity test was performed by using paper diffusion, whileminimum concentration test was done by using dilution method. The result show that compounds ofsambung darah leaves extract were alkaloid, tanin, flavonoid, tritefenoid, glicosida and saponin. Sambungdarah extract has antibacterial activity on Gram positif bacteria (S. aureus, S. epidermidis, S agalactiae),where as it has not antibacterial activity against Gram-negative bacteria (E. coli and Salmonella sp.).
Virgin Coconut Oil Meningkatkan Aktivitas Fagositosis Makrofag Ayam Pedaging Pascavaksinasi Flu Burung (VIRGIN COCONUT OIL INCREASES THE PHAGOCYTOSIS ACTIVITY OF MACROPHAGE OF BROILER CHICKEN FOLLOWING AVIAN INFLUENZA VACCINATION) Enny Yusuf Wachidah Yuniwarti; Widya Asmara; Wayan Tunas Artama; Charles Rangga Tabbu
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The research objective was to find an alternative avian influenza prevention in broilers by increasinganimal’s antibody titer and macrophages phagocytic  activity.  Virgin coconut oil (VCO) is a food supplementthat is proven safe for human consumption therefore it is assumed to be safe for the animal’s (chickens).Factorial design  2 vaccinated: unvaccinated) x 4 (dose of VCO: 0, 5, 10 and 15 mL/kg feed) were applied inthis study.  A total of 40 day day old chick were allocated in the eight treatments groups.  Feed and drinkingwater were available  ad libitum.  Antibody titers of the animals were detected using ELISA, whereasphagocytic activity of the macrophages were detected from spleen.  The result showed that the highestphagocytic activity and antibody titers were seen in chickens which were given VCO at 10 mL/kg feed.  It isconcluded that the VCO could increased the phagocytic activity of macrophages.
Karakteristik Morfologi, Perbedaan Jenis Kelamin, dan Pendugaan Umur Burung Weris (Gallirallus philippensis) di Minahasa, Sulawesi Utara (MORPHOLOGICAL CHARACTERISTICS, SEX DIFFERENCES, AND AGE ESTIMATION OF WERIS (GALLIRALLUS PHILIPPENSIS) FROM MINAHASA Lucia Johana Lambey; Ronny Rachman Noor; Wasmen Manalu; Dedy Duryadi
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aim  to observeb Gallirallus philippensis  similarities between different sites in Minahasa,to detect sexual dimorphism, and to estimate the bird age based on morphological characteristics.  A totalof 35 birds  were collected  from four locations i.e. Tondano, Papontolan, Ranoyapo, and Wusa in MinahasaNorth Sulawesi.   The result showed that there were no differences in the length  of beak, wing, and shankbetween  birds from  the different locations.  Moreover, based on the Principle Component Analysis (PCA)the bird’s shank and tail’s length were the main component.  Based on cluster analysis, the birds from fourlocations had a close similarities.  The male bird’s body weight, beak length, and beak width were largercomponent to the female’s.  The bird’s age could be best predicted by their iris colour,  beak colour, feather colourof the neck and the growth of the wing feathers, respectively.
Peranan Zona Pelusida Sebagai Barier Terhadap Cemaran Escherichia coli K99 (THE ROLE OF ZONA PELLUCIADA AS A BARRIER OF E COLI K99 CONTAMINATION) I Wayan Batan; Bibiana Widiati Lay; Ita Djuwita; Supar .; Arief Boediono
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to investigate the role of zona pelucida (ZP) as a barrier of embryo againstE.coli K99 contamination.   The complete randomized design was used in this study.  The embryos weregiven treatment as a follow : 1) embryos without ZP were contaminated with E.coli K99;  2) embryo withintact ZP were contaminated with E.coli K99;  and  3) embryos  with intact ZP were not contaminated withE.coli K99, as a control.  In each treatment there was 15 replication and in each replication there was oneembryo.  The embryos were incubated in incubator at 37°C and 5% CO2 atmosphere.  The embryos wereobserved every six hours in 24 hours using inverted microscope.  The result showed that embryos withintact ZP could develop in culture contaminated with  E.coli K99, while embryos without ZP becomedegenerated.  The viability of intact embryos was 75% and the embryos without ZP were 65%.  Embryosculture in contaminated medium could develop from eight cells embryo into morulla stage of embryo,compact morulla, and blastocyst. E.coli K99 contamination could inhibit embryo development.  In conclusion,ZP could protect embryo against E.coli K99 contamination.
Induksi Ekstrak Pegagan Secara in vitro terhadap Proliferasi dan Diferensiasi Sel-Sel Otak Besar Anak Tikus (IN VITRO INDUCTION OF CENTELLA ASIATICA (PEGAGAN) EXTRACT ON THE PROLIFERATION AND DIFFERENTIATION OF NEWBORN RAT CORTEX CEREBRI CELLS) Ita Djuwita; Min Rahminiwati; Latifah Kosim Darusman; Siti Sa’diah
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to analyze the potency of Centella asiatica extract to induce proliferation andneurogenesis process of newborn rats cortex cerebri.   Research has been conducted on in vitro culture ofthree days old rat (Sprague Dawley) cerebrum cells in DMEM (Dulbecco’s Modiûed Eagle’s Medium)containing 10% NEAA (Non Essential Amino Acid), 1 mM NaHCO3, 10% NBCS (Newborn Calf Serum)and 50 µg/mL gentamycin (mDMEM), with and without Centella asiatica (CA) leaf extracts. The experimentwas set in five groups of treatment consisted of positive control (mDMEM+30 µg/mL asiaticoside (AC)),negative control (mDMEM), and mDMEM with three concentration of CA extract i.e. 100 ppm, 200 ppmand 400 ppm. Culture was done in 5% CO2 incubator at 37oC for six days. The parameters observed werecells proliferation based on Population Doubling Time (PDT), neuron and glia composition, and the lengthof axon and dendrite. Cells concentration were counted using Newbauer hemocytometer.  Neuron and gliacells were determined based on morphology after Hematoxylin-Eosin staining, and the length of axon anddendrite were measured using eyepiece micrometer. Data were analyzed using ANOVA and Duncan test.The results showed that Centella asiatica extract at concentration 100 ppm could induce neurogenesisand increased the axon length growth.  However, at concentration 200 and 400 ppm, CA extract  inhibitedthe neuronal cells proliferation and the axonal growth (P<0,05). In conclusion, induction of Centella asiaticaextracts at concentration of 100 ppm on the cortex cell cerebrum cells culture increase the axon lengthgrowth and tends to induce neurogenesis; however at higher concentration CA extract was neurotoxic.
Faktor Risiko Terkait Manajemen Kesehatan Unggas terhadap Infeksi Virus Flu Burung di Tempat Penampungan Ayam (THE RISK FACTOR OF POULTRY HEALTH MANAGEMENT TO THE INFECTION OF AVIAN INFLUENZA VIRUS IN POULTRY COLLECTING FACILITIES) Chaerul Basri; Zudanang .; Sunandar .; Etih Sudarnika
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to determine the association between the implementation of poultryhealth management and the spread of avian influenza virus in Poultry Collecting Facilities (PCFs).  Thestudy was performed by maintaining 7-8 sentinel chickens in 39 PCFs in Jakarta for three months.  Thevariables evaluated for poultry health management were health certificate, health inspection, healthinspector, health inspection method, and handling of sick and dead birds. Data on the variables werecollected by interview with supervisor of PCFs.  The AIV infection were detected by rt-PCR from the cloacaland tracheal swab of the dead birds with.  The results showed that the methodes of handling of sick birdswere significantly associated with infection of AIV (RR=2,00 ; 95% CI  = 1,31-3,05).  The other variables didnot show significance association.  The risk of AIV infection was twice higher  the sick keeping side birdsalive, or by separating, or treating the birds in the same cage than by slaughtering them. Poultry healthmanagement in PCFs need to be improved in order to prevent and control the spreading of AIV in Indonesia.

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