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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 19 Documents
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Search results for , issue "Vol 15 No 1 (2014)" : 19 Documents clear
Pemberian Fikosianin Spirulina Meningkatkan Jumlah Sel Darah, Aktivitas Fagositosis, dan Pertumbuhan Ikan Kerapu Bebek Juvenil (ADMINISTRATION OF SPIRULINA PHYCOCYANIN ENHANCES BLOOD CELLS, PHAGOCYTIC ACTIVITY AND GROWTH IN HUMPBACK GROUPER JUVENILE) Woro Hastuti Satyantini; Sukenda .; Enang Harris; Nur Bambang Priyo Utomo
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to investigate effects of Spirulina phycocyanin on the total  blood cell count,phagocytic activity, and growth of humpback grouper fish, Cromileptes altivelis juvenil.  Fishes were fedwith a diet containing   0, 150, 250, 350 dan 450 mg  phycocyanin per kg diet for four weeks and eachtreatment was triplicates.  Initial body weight  of  grouper was  8.46 ± 0.22 g with a density of 10 fish per56 litre volume. The total count of  erythrocytes and leucocytes increased until the fourth week of rearingperiod. The highest of total erythrocyte and leucocytes were observed in fish treated with 150 mg phycocyaninper kg diet ( 13.17 x  105 cells/mm3 and 8.93 x 105 cells/mm3 respectively) which were not significantlydifferent (P>0.05) to those treated with 250 mg phycocyanin per kg diet. The total leucocytes and phagocyticactivity of fish fed diet containing  250 mg phycocyanin  per kg diet (8.49 x 105 cells/mm3 and 59.67%respectively) were significantly higher  (P <0.05) to those of control group. The highest of final weight(Wt=14.32 g) and weight growth (G=5.89g) and lowest of feed conversion ratio (FCR=1.13) were obtainedin fish treated with  250 mg phycocyanin per kg diet which were  significantly  higher  (P <0.05) than thosefed control diet. The data showed that  the addition of  phycocyanin 250 mg/kg diet enhances the totalleukocyte count, phagocytic activity and the growth of humpback grouper juvenil.
Kepekaan Telur Spesific Pathogen Free dan Clean Egg Terhadap Virus Flu Burung (SENSITIVITY OF SPESIFIC PATHOGEN FREE EGGS AND CLEAN EGG TO THE AVIAN INFLUENZA VIRUSES SUBTYPE H5N1) Gusti Ayu Yuniati Kencana; I Nyoman Suartha; Arini Nurhandayani; Muh Ramadhan
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian Influenza which is  in Indonesia  known as Flu Burung  is caused by the avian influenza virussubtype H5N1 (AIV-H5N1). Vaccination is one of the major strategies for preventing and eradicatingAIV-H5N1 in Indonesia. Several factors can affect the potential vaccine such as viral content and mediaused for the propagation of the virus. One of the media commonly used to propagate  the virus is pathogenspecific free (SPF) embryonated chicken eggs. However, as the SPF eggs production is limited and expensive,the use of clean embryonated chicken eggs as an alternative need to examined. This study aimed todetermine the sensitivity of SPF and clean embryonated chicken eggs to the AIV-H5N1. The virus usedwas seed avian influenza virus (A/ Chicken/West Java (Subang)/29/2007)  which haa previously werepropagated  in SPF eggs and the Clean Eggs. The virus titer was determined as Embryo infective Dose 50%(EID50) using Reed and Muench method. Sensitivity of SPF eggs and Clean Egg to the VAI-H5N1 wascompared using  Chi-square statistical analysis. The titers of Avian Influenza Virus subtype H5N1 were106.83EID50/0.1ml in SPF eggs and 106.17EID50/0.1 ml in the Clean Eggs. Statistical analysis showed that,the sensitivity of SPF Eggs and Egg Clean  for the propagation of the VAI-H5N1 was not significantlydifferent.
Kualitas Semen Cair Kambing Peranakan Etawah dalam Modifikasi Pengencer Tris dengan Trehalosa dan Rafinosa (THE QUALITY OF ETAWAH CROSSBREED BUCK LIQUID SEMEN IN MODIFIED TRIS DILUENTS WITH TREHALOSE AND RAFFINOSE) Oriza Savitri Ariantie; Tuty Laswardi Yusuf; Dondin Sajuthi; Raden Iis Arifiantini
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to compare the efficacy of trehalose and raffinose supplementation in Trisegg yolk (TEY) and Tris soya (TS) diluents in optimizing the quality of Etawah Crossbreed liquid semen.Semen were collected from three sexually mature bucks using artificial vagina. Semen were then evaluatedand divided into six aliquot tubes. Each of them was diluted in TEY and TS supplemented with 50 mMtrehalose or raffinose, respectively. The liquid semen were then stored in refrigerator (5°C). The motility,viability and plasma membrane integrity (PMI) of the spermatozoa were evaluated every 12 hours untilsperm motility remained up to 50%. The results showed that sperm motility in TEY supplemented withtrehalose or raffinose remained up to 50% for 72-84 hours, compared to in TS which was for 48-60 hours(P<0,05). The best diluent was demonstrated by TEY supplemented with trehalose where the spermmotility was 52,82±3,21% up to 84 h compared to raffinose supplementation (52,78±4,41%) and control(51,78±4,86%) which was up to 72 hours, respectively. Meanwhile, the spermatozoa motility in TS diluentsupplemented with raffinose was 52,78±4,41% for up to 60 hours compared to supplemented with trehalose(53,33±3,54%) and control (51,11±4,86%) which was up to 48 hours. In all diluents, the viability ofspermatozoa was 6-9% higher than the percentage of sperm motility, whilst the percentage of PMI wassimilar to the percentage of sperm motility. In conclusion, TEY supplemented with trehalose was the bestdiluents for preservation of Etawah Crossbreed buck liquid semen, and when using TS diluent it isrecommended to add raffinose  rather than trehalose.
Pemberian Kalsium Laktat dan Berenang Meningkatkan Osteoblast pada Epiphysis Tulang Radius Mencit Perimenopause (ORALLY LACTATE CALCIUM AND SWIMMING INCREASE OSTEOBLAST IN EPIPHYSIS RADIAL PERIMENOPAUSE MICE BONE) Muliani .; I Nyoman Mangku Karmaya; Ketut Tirtayasa
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study is to measure the effect(s) of orally administered calcium lactate and swimmingactivity to the increase of osteoblasts in the epiphysis of radial bone at perimenopause stage. We usedmice of Mus musculus species age 15-16 months as experimental animals in this particular study. Forexperimental design we used Pre-test and Post-test Control Group Design. There were 4 experimentalgroups: Control; Calcium lactate administration; Swimming activity; and Combination of calcium lactateand swimming activity. Each group contained 22 mice and treatments were given to each group for sixweeks (42 days). Our results showed that following treatment, the average numbers of osteoblasts were9.46±1.81; 26.58±2.82; 25.05±2.68; 59.38±2.91 in Control, Calcium lactate administration, Swimmingactivity, and Combination of calcium-lactate administration and swimming activity groups respectively.There was a significant difference in average osteoblasts between the Control group and the other 3 groups(p<0.05) and there was no significant difference between the Calcium lactate administration and Swimmingactivity groups (p>0.05). The increase number of osteoblasts in the Combination group was larger than theother groups. In conclusion, administration of calcium lactate or swimming activity could increase thenumber of osteoblasts in mice, and the increase is larger when applied in combination.
Peningkatan Kandungan Kalium Urin Setelah Pemberian Ekstrak Sari Buah Belimbing Manis (Averrhoa carambola) (THE INCREASE OF POTASSIUM URINE CONTENT AFTER ADMINSTRATION OF CARAMBOLA (AVERRHOA CARAMBOLA) FRUIT JUICE EXTRACT) Ruqiah Ganda Putri Panjaitan; Maria Bintang
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Carambola (Averrhoa carambola L.) has been used as medicinal plant. This research has beenconducted to study the potential diuretic of fruit juice carambola extract on male rats. Diuretic activitywas tested by using Cumming’s method. The treatment was administered only once, and the urine up to 24hours after treatment was collected. The result shows that the administration of 1.6 mL/100 g body weightof fruit juice carambola extract resulted in lower urine volume compared to the without treatment orklortalidon at dose 0.315 mg/100 body weight (p>0.05). Furthermore, Na+ content in treatment rats’ wasurine lower compared to the without treatment or klortalidon (p<0.05). in contrast, high content of K+ wasobserveb in treatment  rast’ urine compared to the without treatment or klortalidon (p> 0.05). It is concludedthat the administration of carambola fruit juice extract may increase K+ content in urine and produce moreconcentrated urine. The mechanism of action, however, remains need to be proven, further.
Real Time Polymerase Chain Reaction : Perangkat Diagnostic Alternatif untuk Melacak Virus Nipah (REAL TIME POLYMERASE CHAIN REACTION : AN ALTERNATIVE DIAGNOSTIC TOOL TO DETECT NIPAH VIRUS) Indrawati Sendow; Atik Ratnawati; Raden Mas Abdul Adjid; Muharam Saepulloh
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Nipah is a dangerous zoonotic disease with a high social, economical and psychological impact. Fruitbat Pteropus sp. is one of the nipah virus  reservoir host. As the virus is categorized as a dangerous zoonoticdisease that cause fatal in human, all works related to live virus should be conducted in a laboratory withBSL4 facilities. The detection of nipah virus using real time PCR to replace virus isolastion can thereforebe conducted in a laboratory without BSL4 facilities. The results was further  confirmed at referencelaboratory at   Australian Animal Health Laboratory ( AAHL) Geelong, Australia, indicated that nipahvirus can be detected in saliva of fruit bat P. vampyrus in Medan North Sumatera.
Praktek Sanitasi Higiene pada Usaha Pengolahan Dangke Susu Sapi di Kabupaten Enrekang, Sulawesi Selatan (THE PRACTICES OF HYGIENIC SANITATION IN PROCESSING INDUSTRIES OF COW MILK DANGKE IN ENREKANG DISTRICT, SOUTH SULAWESI) Wahniyathi Hatta; Mirnawati Bachrum Sudarwanto; Idwan Sudirman; Ratmawati Malaka
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Lack of hygienic sanitation conditions in food processing industry could contribute to the food safety.The aim of this study was to assess the implementation of hygienic sanitation practices in the processingindustries of cow milk dangke in Enrekang District, South Sulawesi. Total of 60 respondents wereinterviewed using questionnaire and observation on hygienic sanitation practices was carried out usingcheck-list.  Respondents were producers and also workers in the processing units in Cendana Sub-district,Enrekang which had been selected with simple random sampling technique.  Scoring of hygienic sanitationcondition were based on the application of hygienic sanitation practices on the checklist.  None of workerswore special clothes, put dangke molds in closed container, and wiped packaging of dangke with specialclothes.  Less than 50% of the workers  wore a head coverings, prepared papaya latex with boiled water,and covered dangke when molding.  A total of 50% of the workers immediately washed the molds afterused.  More than 50% of the workers washed their hands with soap before dangke processing, washed themolds with soap, wiped the packagings with a cloth before it used, stored the milk in a closed container,and cleaned papaya fruit before preparing papaya latex.  All of the workers made dangke while doinghousework. Hygienic sanitation condition of dangke processing industries that had good category was 3%while another 57% in moderate category and poor category was 40%.  The hygienic sanitation practices hadnot been fully implemented on the processing industries of cow milk dangke in Enrekang district, which ledto the category of hygienic sanitation condition of largely of them was moderate .
Identifikasi Flu Burung H5N1 pada Unggas di Sekitar Kasus Flu Burung pada Manusia Tahun 2011 di Bekasi (AVIAN INFLUENZA H5N1 IDENTIFICATION IN AVIAN SPECIES SURROUNDING AVIAN INFLUENZA H5N1 HUMAN CASES IN BEKASI, WEST JAVA, 2011) Dyah Ayu Hewajuli; Ni Luh Putu Indi Dharmayanti
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

H5N1 subtype Avian Influenza (AI) virus is the causal agent  of AI disease in humans. In Indonesia,the first human AI occurred in Tangerang 2005.  Human AI in Indonesia has now spread into 12 provinces,including West Java, Jakarta, Banten, North Sumatra, East Java, Central Java, Lampung, South Sulawesi,West Sumatra, South Sumatra, Riau, and Bali. Until 2011, the total human AI cases were 182 cases  with150 deaths. This study was conducted to identify of H5N1 AI virus in birds in area surrounding a humanAI human case  in Bekasi city  in March 2011 and to investigate its role in the spread of AI to humans usingmethods of Hemaglutination Inhibition (HI ), and Reverse Transcriptase-Polymerase Chain Reaction(RT-PCR). The result showed that 80% of birds in the area surrounding AI  surrounding H5N1 AI humancase in Bekasi 2011 were antibody negative  against  H5N1-AI virus. Antibody against H5N1-AI viruswith the titer less than 4 log 2 was detected in 4.4%  of birds and  with antibody titer 04 4-7 log 2 in 15%of birds. By RT-PCR, H5N1 AI virus was not detected in 47.6% of bird samples. H5 positive and N1negative  AI virus was detected in  30.2% samples.  Only 11.2% samples showed positive for H5N1 AI virus.The results suggest that H5N1-AI virus affecting birds may have a positive role in transmitting to thevirus to human in Bekasi 2011.
Pendugaan Produksi Karkas Dan Daging Kelelawar Pemakan Buah (Pteropus alecto ) Asal Sulawesi (ESTIMATION OF CARCASS AND MEAT PRODUCTION OF CELEBES NATIVE FRUIT BATS (Pteropus alecto)) Tiltje Andretha Ransaleleh; Rarah Ratih Adjie Maheswari; Purwantiningsih Sugita; Wasmen Manalu
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The study was conducted in North Sulawesi, Gorontalo, and Central Sulawesi during March untilOctober 2011. The purpose of this study was to determine the potential of fruit bats  (P. alecto) as sourceof meat. Observed variables were body weight, carcass weight, noncarcass weight, the bone weight, meatweight, fat weight, and skin weight, respectively. To estimate the growth rate of the bats, analysis of therelationship between body weight and carcass components was done, while estimation of  growth rate ofcarcass components were analyzed by multiple linear regression. The results showed that the carcassproduction of  P. alecto in the three locations was 54.49%-56.55%, meat production was 45.37% -54.07%,and the coefficient of determination was 0.65-0.99.  Conclusions of this study is that the body weight  canbe used for  prediction  of growth rate, and weight of carcass, meat, bone, fat, and skin of the fruit bats,respectively.
Perkembangan Praimplantasi Embrio Mencit dengan Materi Genetik yang Berasal dari Parental, Maternal, dan Inti Sel Somatik (PRE-IMPLANTATION DEVELOPMENT OF MOUSE EMBRYO WITH GENETIC MATERIAL DERIVED FROM PARENTAL, MATERNAL AND SOMATIC CELL NUCLEUS) Harry Murti; Mokhamad Fahrudin; Mohamad Agus Setiadi; Boenjamin Setiawan; Arief Boediono
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cloned embryo and parthenogenetic embryo are a potential source of stem cells for regenerativemedicine. Stem cells derived from those embryos are expected to overcome the ethical issues to the use offertilization embryos for therapeutic purposes. The pre-implantation development is a critical step fordeveloping embryos reach the blastocyst stage. The objectives in vivo of this research are to produce mousecloned embryo, parthenogenetic embryo, and fertilized embryo and to study stages of  in vitro pre-implantation development culture. In vivo fertilized embryos, mouse oocytes, and cumulus cells were usedin this study. Treatment was performed on female mice superovulated with PMSG and hCG injections.Two-cell stage of in vivo fertilized embryos were collected on the second day post hCG injection. Clonedembryos were produced through Somatic Cell Nuclear Transfer (SCNT), which included enucleation, nucleartransfer and artificial activation. Parthenogenetic embryos were produced with artificial activationtechnique. The result of the research indicated that SCNT application was able to produce cloned embryos which could develop to blastocyst stage (3,2%). In addition, artificial activation of oocytes could produceparthenogenetic embryos which were able to develop up to the blastocyst stage (8,6%). In conclusion,efficiency level of parthenogenetic embryos that is able to reach the blastocyst stage was higher than in thecloned embryos. Fertilized embryos shows a better development and more efficient compared to in vitrocloned embryos and parthenogenetic embryos cultures.

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