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I G. Made Krisna Erawan
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krisnaerawan@unud.ac.id
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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia
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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 19 Documents
Search results for , issue "Vol 16 No 2 (2015)" : 19 Documents clear
Respons Antibodi terhadap Penyakit Tetelo pada Ayam yang Divaksin Tetelo dan Tetelo-Flu Burung (NEWCASTLE DISEASE/ND ANTIBODY RESPONSE OF CHICKENS VACCINATED WITH ND SINGLE AND COMBINED ND AND AVIAN INFLUENZA VACCINES) Gusti Ayu Yuniati Kencana; Nyoman Suartha; Mesakh Parlindungan Simbolon; Arini Nur Handayani; Steffi Ong; Syamsidar .; Aprillia Kusumastuti
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to investigate antibody response of specific pathogen-free (SPF) chickens vaccinatedwith single inactivated Newcastle disease (ND) vaccine and combined inactive ND and avian influenza(AI) vaccines and to known the efficacy of both vaccines. The vaccines used were killed ND vaccine andkilled ND-AI vaccine produced by PT. Sanbio Laboratories Bogor, West Java. SPF chickens were vaccinatedwith 3 different doses. Antibody titer of SPF chickens against ND virus were determined byhaemagglutination inhibition (HI) test. As many as 130 two week old SPF chickens were used and theywere divided into 2 groups (A and B) consisting of 60 chickens and 10 chickens were used as control withoutvaccine. Group A chickens were vaccinated with ND-K vaccine and group B were vaccinated with combinedkilled ND-AI vaccines. Each group was further divided into 3 subsgroups (1, 2 and 3) consisting 20 chickens.Subgroups 1, 2 and 3 were vaccinated intramuscularly respectively with intramuskular 1, 1/10 and 1/100doses of each vaccines. Antibody response of chickens against ND virus was examined before vaccinationand every three week after vaccination and was expresses as geometric mean titre (GMT) HI units. Theresult showed that the titre antibody against ND increased at the second week following the vaccination.The antibody titer against ND virus of chickens vaccinated single killed ND at the second week in eachdose were 6.05 GMT HI unit, 4.05 GMT HI unit, and 0.9 GMT HI unit. The antibody titre at the third week were 7.90 GMT HI unit ,5.40 GMT HI unit and 2.20 GMT HI unit. The antibody titre against ND virus ofchickens vaccinated with combined ND-AI vaccine at the second week were 6.30 GMT HI unit , 4.15 GMTHI unit , and 2.05 GMT HI unit. At the third week, the antibody titre against ND virus of chickensvaccinated with combined ND-AI vaccine in each subgroup were 7.45 GMT HI unit, 5.60 GMT HI unit , and2.40 GMT HI unit . It showed that the antibody titers at single doses of killed ND vaccine (7.90 GMT HIunit) and combined ND-AI vaccine (7.45 GMT HI unit) at the third week after vaccination were both stilleffective as both titres were above standard protective titre.
Respons Imun Mencit yang Diimunisasi dengan Cysticercus cellulosae (IMMUNE RESPONSE TO TAENIA SOLIUM CYSTICERCOSIS IN MICE) Ida Bagus Ngurah Swacita; I Made Damriyasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; Ida Ayu Pasti Apsari; I Wayan Masa Tenaya
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cysticercosis is a zoonotic parasitic disease which is still problem in Indonesia. The purpose of thisstudy was to investigate immune responses of mice that had been immunized using Taenia solium larval(Cysticercus cellulosae) antigens originated from infected pigs. Three kinds of the C.cellulosaeantigens,secretory and whole antigens were used to immunize three different groups Balb/c mice consisted of 15animals. The serum samples before and after immunization were tested with ELISA test. The resultsshowed that the third antigens induced highly significant titre (P<0,01)compared to unimmunized animals.However no significant different (P>0,05) were found when the third antigens were compared. It wasconcluded that immunization with the three kinds of C. cellulosae antigens can cause immunity in mice.
Karakterisasi Lactobacillus spp. yang Diisolasi dari Susu Kambing Etawa untuk Pengembangan Probiotik (CHARACTERIZATION OF LACTOBACILLUS SPP., ISOLATED FROM MILK OF ETAWA GOATS FOR LOCAL PROBIOTIC DEVELOPMENT) Putu Rima Sintyadewi; Yan Ramona; I Nengah Sujaya
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this research was to characterize Lactobacillus spp., isolated from milk of Etawagoats for local probiotic development. Total of 23 isolates Lactobacillus spp. were tested for resistance tolow pH conditions, high levels of natrium deoxy cholate, and modified gastric juice conditions. Besidesthat, those isolates were also tested to convert cholic acid (CA) into deoxycholic acid (DCA). Isolate thatshowed the most potential properties for local probiotic development was identified by 16S rDNA analysisusing following amplification of this sequence with primers of 27F and 520R). The results showed that 12isolates were found to be resistant to low pH conditions and to high level of NaDC (0.6 mM). Three of them(Lactobacillus spp. GMA46, Lactobacillus spp. GMA47 and Lactobacillus spp. GMA50) did not convertcholic acid into deoxy cholic acid, indicating that they are safe for human use. Lactobacillus spp. GMA46showed better performance in the gastric juice (a model of gastic and intestinal juice containing pepsin andpancreatin enzymes at pH 2, 3 and 4) simulation test. This GMA46 isolate was identified as L. caseiATCC 334 and L. paracasei subsp. tolerans strain NBRC 15906 with 100% similarity, in term of its 16srDNA nucleotide sequence. The results of this research indicate that Lactobacillus sp. GMA46 is anIndonesian potential probiotic strain, isolated from milk of etawa goats
Kemampuan Ekstrak Jamur Lingzhi dalam Menghambat a-Glucosidase dan Menurunkan Kadar Gula Darah pada Tikus Hiperglikemia (THE ABILITY OF LINGZHI MUSHROOM EXTRACT (GANODERMA LUCIDUM) IN INHIBITING a-GLUKOSIDASE AND ITS EFFECT ON HIPERGLYCEMIAOF RATS) Ratih Dwi Indriani; I Nyoman Suarsana; I Wayan Sudira
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Lingzhi mushrooms has been widely used by community empirically for the traditional medicine forlowering blood glucose levels. The purpose of this study was to determine the ability of the lingzhimushroom (Ganoderma lucidum) extract to inhibit á-glukosidase hyperglycemia in rat. Lingzhi mushroomethanol-extract were tested for their inhibition against á-glucosidase using spectrophotometry method. Atotal of 25 rats used in this study were divided into five groups. Four groups of rats (P1-P4) were given 2 mlof sucrose 80% orally, and one group of rats (P0) as a negative control. Ten minutes before giving sucrose,three groups (P2-P4) of rats were given the lingzhi mushroom extract of 1 ml orally at a dose of 10%, 20%,and 30% respectively, while the second groups (P1) as a positive control of hyperglycemia. After that in allgroups were examined the blood glucose levels at 0, 10, 30, 60, 90, and 120 minute after sucroseadministration. The results showed, lingzhi mushroom extract has the ability to inhibit á-glucosidasewith IC50 value of 162,01 ìgmL-1. The result of blood glucose test showed that administration of lingzhimushroom extract at a dose of 30% faster lowering blood glucose levels compared with a dose of 10% and20%.
Morfologi Anatomi dan Histologi Usus Biawak Air (Varanus salvator) THE ANATOMICAL AND HISTOLOGICAL MORPHOLOGY OF INTESTINAL WATER MONITOR (VARANUS SALVATOR) Hamny .; Sri Mulyani; Dian Masyitha; Sri Wahyuni; Muhammad Jalaluddin
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to determine the anatomical morphology and histology of the intestineof water monitor (Varanussalvator). Intestine organ from an adult male water monito was used in thisstudy. Perfusion was performed to flow the 4% paraformaldehyde fixative solution through the heart.Intestine was collected, then divided into six sections, followed with dipping them into 4% paraformaldehydesolution for four days, continued histological preparations and stained with haematoxylin eosin (HE). Theanatomical morphology observation was performed before the intestine was processed into histologicalpreparations by observing the outer shape of the intestine and measuring it’slength. Morphologicalobservation was made †after processing theintestinal tissue by observing each portion of the intestinalwall structure. The results showed that transverse fold were found on the outer and inner surfaces of theintestine section I-IV, while in the V-VI part of the intestine, the folds were not observed anymore. Theintestine wall consisted of four layers, such as mucosa, submucosa, tunica muscularis, and serosa. Thevilli on intestine section I-IV were relatively longer and higher, while the intestine vili in section V-VI wererelatively short. The entire mucosa in water monitor was coated with absorptive columnar epitheliumwith goblet cells. Based on the result study, it can be concluded that the water monitor has more efficientdigestive process which can be seen morphologically from the relatively short intestinal, has transversefolds on the surface of the small intestine, and does not have cecum. The intestinal wall consists of layersi.e : mucosa, submucosa, tunica muscularis, and serosa.
Karakteristik Semen dan Kadar Testosteron Berdasarkan Ukuran Lingkar Skrotum Kambing Kejobong Muda dan Dewasa (CHARACTERISTICS OF SEMEN AND TESTOSTERONE LEVELS BASED ON SCROTUM CIRCUMFERENCE SIZE IN YOUNG AND ADULT KEJOBONG BUCKS) Ono Syamyono; Daud Samsudewa; Enny Tantini Setiatin
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aims of this research were to investigate the characteristics of semen and testosterone levels based onthe different scrotum circumference size in young and adult Kejobong bucks. The samples in the study usedwere 35 Kejobong bucks, consisted of 20 young bucks (8 months-<1 year; BW 32.52 ± 5.58 kg) and 15 adultbucks (1-2 years; BW 37.83 ± 5.95 kg). The young bucks were divided into two group categories, i.e.: scrotalcircumference size of 19.1 to 22.0 cm (M1) and 22.1 to 25.0 cm (M2). The adult bucks were also divided intotwo group categories, scrotal circumference size of 21.1 to 24.0 cm (D1) and 24.1 to 27.0 cm (D2). Surveymethod was used and the samples were determined by using purposive sampling. Testosterone levelswere measured using the ELISA test. The data were analyzed descriptively and the scrotal circumferencesize comparison was analyzed with the t-test. The results showed that the characteristics of semen andtestosterone levels in young Kejobong bucks of M1 and M2 groups were not significant (P>0.05). Similarlythe characteristics of semen and testosterone levels in adult Kejobong bucks of D1 andD2 groups were alsonot significant (P>0.05). These can be concluded that different scrotal circumference size of young andadult Kejobong bucks had similarity characteristics of semen and testosterone levels.
Seroprevalensi Positif Sistiserkosis pada Babi Hutan di Kabupaten Way Kanan, Provinsi Lampung (POSITIVE SEROPREVALENCE OF WILD BOAR CYSTICERCOSIS IN WAY KANAN DISTRICT, LAMPUNG PROVINCE) Heri Yulianto; Fadjar Satrija; Denny Widaya Lukman; Mirnawati Sudarwanto
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Wild boar is also an intermediate host of Taenia solium, besides domestic pig which is the mainsource of Cysticercus infection in human. Cysticercosis in wild boar has not been reported in Indonesia.The aim of this study was to determine wild boar’s cysticercosis in Way Kanan District. One hundred serasamples consisting of 41 male wild boars and 59 female wild boars were collected. Eighty seven of boarscome from Banjit Subdistrict, while 13 of wild boars come from Blambangan Umpu Subdistrict in WayKanan District. The serum were tested serologically to determine antigen of parasite cycles. The test usedmonoclonal antibody-base sandwich Enzyme-Linked Immunosorbent Assay (Moab-ELISA). There is asero positive reaction of cysticercosis(1%) which is female wild boar from Banjit sub district. Controllingof meat inspection should be conducted to prevent cysticercosis transmission to human.
Identifikasi Keragaman Genetik Gen 12S Ribomsom RNA Sebagai Penanda Genetik untuk Penentuan Spesies Kuskus (IDENTIFICATION OF GENETIC DIVERSITY 12SRRNA GENES AS GENETIC MARKER FOR DETERMINING SPECIES CUSCUS) Rini Widayanti; Hery Wijayanto; Woro Danur Wendo; Rony Marsyal Kunda
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cuscus is marsupial’s animal (Phalageridae) which has limited spread in eastern Indonesia (Sulawesi,Maluku, Papua), Australia and Papua New Guinea. The ex-situ and in-situ conservation of cuscus undercaptivating condition is an alternative solution to protect cuscus from extinction. This study aimed todetermine nucleotide sequence and genetic markers on 12Sr RNA gene with sequencing method of eachspecies on three islands. Whole genome DNA was extracted from 17 samples of cuscus obtained fromdifferent habitats, Sulawesi (2 individual), Maluku (7 individual), and Papua (8 individual) according tothe protocol of Qiamp DNA Blood Mini Kit (Qiagen), and then it was used as template for amplificationof 12Sr RNA gene by using PCR. PCR product were then purified using column chromatography and wereused as template for sequencing reaction. Result sequencing of 12Sr RNA gene were analyzed usingMEGA program version 6. PCR product gives a result nucleotida of 958 bp according to databasegenebank, sequencing product gives result nucleotida of 896 bp and found of 105 different nucleotide sites.Filogram based on nucleotide sequences 12SrRNA gene from Sulawesi cuscus is Ailurops ursinus whereasthe cuscus from Papua and Maluku is Phalanger sp. and Spilocuscus maculatus species. Thirteen nucleotidasites were found, sites no 67 (A/G), 89 (G/C), 137 (T/C), 285 (G/A), 468 (T/C), 595 (T/C, 598 (T/C), 647 (T/C),654 (G/A), 665 (T/C), 769 (C/T), 874 (C/T), and 876 (A/G) which can be used as genetic marker betweenPhalanger genera from Papua and Maluku, and three nucleotida sites (sites no 127 (G/A), 481 (C/T), and885 (T/C) can be used as genetic marker between Spilocuscus genera from Papua and Maluku.
Pengembangan Enzyme-Linked Immunosorbent Assay Paratuberkulosis dengan Antigen Protoplasmik Mycobacterium avium Subspecies Paratuberculosis Isolat Lapang (DEVELOPMENT OF PARATUBERCULOSIS ENZYME-LINKED IMMUNO-SORBENT ASSAY WITH PROTOPLASMIC ANTIGEN OF MYCO Rahmat Setya Adji; I Wayan Teguh Wawan; Denny Widaya Lukman; Surachmi Setiyaningsih
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Enzyme-linked immunosorbent assay (ELISA) is a serological test method most widely used for thediagnosis of paratuberculosis, because it has a better sensitivity compared to other serological test.Protoplasmic antigen (PPA) or cellular extract is still the main choice for the diagnosis of paratuberkulosisdevelopment. The aim of research was to use the PPA Mycobacterium avium subspeciesparatuberculosis(MAP) field isolates for the development of paratuberculosis ELISA (ELISA PPA-L). As many as 322cattle sera (300 negative and 22 positive) were tested using this method and compared with IDEXXcommercial kit. The sensitivity and specificity of ELISA PPA-L test results were 68.18% and 97.0%,whereas for the IDEXX kit were 63.64% and 97.33%respectively. ELISA PPA-L had higher sensitivity andlower specificity compared to the IDEXX commercial kit. ELISA test using protoplasmic antigen of MAPfield isolates has good ability for paratuberculosis serological test and can be used for screening test of thedisease in Indonesia.

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