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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 23 Documents
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Search results for , issue "Vol 17 No 4 (2016)" : 23 Documents clear
Karakteristik Parsial Gen Sitokrom-C Oksidase Subunit-I Katak Pohon Suaka Marga Satwa Tanjung Peropa Moramo, Sulawesi Tenggara (CHARACTERISTICS OF PARTIAL CYTOCHROME C OXIDASE SUBUNIT I (COI) GENE OF TREE FROG (Polypedates celebensis) IN TANJUNG PEROPA WI Suriana Suriana; Nasaruddin Nasaruddin
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Tree frog (Polypedates celebensis) is an endemic amphibi found in Southeast Sulawesi. Database ofthis species, in particular genetic databases is still very limited. The purpose of this research was tocharacterize the partial gene cytochrome C oxidase subunit I (COI) of tree frog (P. celebensis). Frog sampleswere collected from Tanjung Peropa Wildlife Reserve, Moramo, Southeast Sulawesi, and extracted theirDNA genome, and amplified using the polymerase chain reaction (PCR) method, with specific primers,and then sequenced. Characterization was done by comparing the COI gene with another COI gene fromother frog (data available on GenBank). The results showed that the COI gene fragment P. celebensisconsisted of 665 base pairs. These fragment gene had 99% conserved nucleotides, and only 1% nucleotidesthat varied. Nine sites of nucleotide varied namely: 65th, 98th, 101th, 358th, 396th, 403th, 405th, 424th, and426th. These sites of nucleotide varied can be used as genetic markers for P. celebensis. Composition ofnitrogenous bases P. celebensis COI gene fragment were 29.9% timine, 28.9% cytocine, 22.0% adenine, and19.2% guanine. Molecular phylogeny of P. celebensis based on 665 bp of their COI gene suggests that thesespecies was monophyletic. It is concluded that the gene fragment of cytochrome C oxidase of tree frog (P.celebensis) the origin of Wildlife Moramo, Southeast Sulawesi, has conserved nucleotides more thannucleotides that varied. These varied nucleotides can be used as genetic markers especially for the treefrog origin of Wildlife Moramo, Southeast Sulawesi. The most nitrogenous bases is thymine, while thefewest is guanine. Molecular phylogeny based on cytochrome C oxidase gene is monophyletic
Case of Entamoebiasis in Pigs Raised with a Free Range Systems in Bali, Indonesia (KASUS ENTAMOEBIASIS PADA BABI YANG DIPELIHARA DENGAN CARA DIUMBAR DI BALI, INDONESIA) Kadek Karang Agustina; Anak Agung Gde Oka Dharmayudha; Ida Bagus Made Oka; I Made Dwinata; I Made Kardena; Nyoman Sadra Dharmawan; I Made Damriyasa
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aims were to measure the prevalence of Entamoeba in pigs in Bali and to identify thezoonotic potential species of Entamoeba. A total of 183 pig stool samples from Bali have been examined.The method being used in this study were combination between coproscopy and molecular techniques.Concentration sedimentation with Sodium Acetic Formaldehide (SAF) as a solution was used in thecoproscopy method, while the Polimerase Chain Reaction method was used to amplify DNA of Entamoeba.Extracted sample’s DNA examined by using primers that specifically for Entamoeba: Entam 1 (F) (5’-GTTGAT CCT GCC AGT ATT ATA TG-3’) and Entam 2 (R) (5’-CAC TAT TGG AGC TGG AAT TAC-3), and toidentify the zoonotic potential species of Entamoeba, samples that produce 550 bp in first amplificationcontinued by primers Epolecki1 (F) (5’-TCG ATA TTT ATA TTG ATT CAA ATG-3’) and Epolecki2 (R) (5’-CCT TTC TCC TTT TTT TAT ATT AG-3’). The results showed that 76.6% of samples were positive incoproscopical examination, but 84.7 % produced 550 bp bands on PCR amplification by using generalprimers. All positive samples on the first PCR continued to second PCR used specific primers for E.poleckii as a potential zoonotic disease and all of the samples showed negative results. This datademonstrated that the prevalence of Entamoeba in a traditional pig scavenging systems in Bali was 84.7%but no specific infection infection caused by E. polecki was found.
Effect on Feeding Thymolina Powder in The Carcass Characteristics and Morphology of Small Intestine of Ross 308 Broiler Chickens (PENGIMBUHAN BUBUK THYMOLINA DALAM RANSUM MEMPERBAIKI PERFORMANS DAN MORFOLOGI USUS HALUS AYAM PEDAGING) Seyyedmousa Hosseini; Mohammad Chamani; Alireza Seidavi; Ali Asghar Sadeghi; Zarbakht Ansari-Pirsaraei
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was performed to elucidate the association effect on feeding thymolina powder in thecarcass characteristics and morphology of small intestine of Ross 308 broiler chickens. In the presentexperiment, 320 one day old Ross 308 broiler chickens were used by using completely randomized designwith 4 treatments, 4 replicates, in which each replicate contained 20 broiler chickens. Experimentaltreatments groups were: control, 0.5, 1, and 2% of Thymolina powder in chickens’ diet. Thymolina® is ananti-bacterial powder drug which is made by composing 4 medicinal plants. A significant difference observedamong treatments in each parameter of the intestinal morphology (villus height, villus width, crypt depthand epithelial thickness. The number of goblet cells were not affected. Results of this findings indicatedthat using Thymolina® in the diet of broiler chickens causes improvements in carcass characteristics.Therefore, it may improve the performance and creation of the favorable broiler chickens.
Waktu dan Kemerahan Vulva Saat Inseminasi Buatan Merupakan Faktor Penentu Angka Kebuntingan Sapi di Sumatera Barat (TIME AND REDDISH SIGN OF VULVA DURINGARTIFICIAL INSEMINATION AS A DETERMINANT FACTORS ON CONCEPTION RATE OF COW IN WEST SUMATERA) Zaituni Udin; Ferdinal Rahim; Hendri Hendri; Yulia Yellita
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

An experiment was conducted to evaluate determinant factors on conception rate of local and crossbreedcow at West Sumatera. There were 162 cows of four AI center at parity-0 to 4TH parity (P-4), inseminatedusing frozen semen of four different bulls (simental,brahman, limousine and Bali cattle). All fed similarityat four AI centers and location to observation of sign of estrus in high land and low land in West Sumatera.All data were analyzed using analyzed of variance in Randomized Block Design (RBD) in factorial as afactor A was time of inseminated; parity;sign of vulva,breed and two location as a replication. Resultsofthis study showed that there were no interaction between time of insemination, breed, parity and thereddish sign of vulva (P>0.05) on conception rate. On the contrary,there were significantly different(P>0.05) on conception rate by time of insemination. The highest conception rate wasfound at the end ofestrus 13–18 h (68.91%),local cow (71.82%), P-3 (67.19%) and reddish sign of vulva (74.25%). It wasconcluded that the effects,both inseminationtime and reddish sign of vulva might be the important factorto get the highest conception rate of the cows.
Performans Produksi Ayam Pedaging pada Lingkungan Pemeliharaan dengan Ketinggian yang Berbeda di Sulawesi Selatan (BROILER PRODUCTIONS PERFORMANCE ON THE DIFFERENT BREEDING ALTITUDE IN SOUTH SULAWESI) Anas Qurniawan; Irma Isnafia Arief; Rudi Afnan
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to identify the environmental factors of chickens based on the different altitudebreeding against the microclimatic and performance production in Takalar district, South Sulawesi. Atotal of 180 Lohmann strain chickens consisted of 90 male chickens and 90 female chickens. A randomizedblock design with two factorial patterns was used in this study. The first factor was based on altitude (low<300 mabove sea level, medium 300-600 m, and high >700 m ), the second factorwas based on gender(male and female). Data were analyzed using analysis of variance (ANOVA) and then the significant effectwas followed with Tukey test. The results showed that the altitudehad a significant effect against thephysiological status (rectal temperature), production performance (feed intake), water intake, final bodyweight, and FCR. The male and female chickens had a significant effect against feed intake, water intake,breeding final body weight, and carcass weight. There was interaction between altitude and sex on weightgain performances. The altitudes may influence the effects of physiological status and productionperformance. The production performance of male seems to behigher than the female.
Polimorfisme Protein Serum Darah Induk Sapi Beranak Kembar dan Tunggal pada Sapi Peranakan Ongole dan Keturunan Simental (BLOOD SERUM PROTEIN POLYMORPHISM OF THE COW DELIVERED TWIN OR SINGLE CALVES IN ONGOLE GRADE AND SIMENTAL CROSSBRED) Tri Yuwono; Irene Sumeidiana; Yon Soepri Ondho; Edy Kurnianto
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The twinning cow is presumable have difference in blood protein typology with the single cow. Thestudy investigated blood protein polymorphism in twinning and single of ongole grade and Simmentalcrossbred cows. The study used four twinning versus eight single ongole grade and seven twinning versussix single Simmental crossbred cows. Blood samples were analyzed by using marker ExactPro BroadRange (10-245kDa) and Sodium Dodecyl Sulphate-polyacrilamide Gel Electrophoresis method, to estimatethe loci of albumin (Alb), post-albumin (Pa), ceruloplasmin (Cp), transpherrin (Tf), and amylase-I (Amy-I).The genetic variation was analyzed based on the value of gene frequency, individual heterozigosity andaverage heterozigosity. In this study the twinning ongole grade cows had lower average heterozigosity(0.46) than the single ongole grade cows (0.49). The twinning Simmental crossbred cows had lower averageheterozigosity (0.35) than the single Simmental crossbred cows (0.40). The average heterozigosity ofongole grade cows had higher value than Simmental crossbred cows. The average heterozigosity rangedfrom 0.35-0.49, which was indicated high genetic variation in all cows. Higher gene frequency of allelealbumin A than albumin B influences the twinning trait in ongole grade and Simmental crossbred cows.
Monoclonal Antibodies as Ligands for Purificaion of Rabies virus Proteins from the Brain Tissues of Infected Dogs and Mice (ANTIBODI MONOCLONAL SEBAGAI LIGAND UNTUK PURIFIKASI PROTEIN VIRUS RABIES ASAL JARINGAN OTAK ANJING DAN MENCIT TERINFEKSI) Nyoman Mantik Astawa; Gusti Ayu Yuniati Kencana; Ida Bagus Suardana
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Immunoaffinity chromatography using monoclonal antibodies (mAbs) as ligands has been used topurify rabies virus (RV) individual proteins. In this method, mAbs against RV were firstly purified,coupled to CnBr-agarose resin and used for purification RV individual proteins. Brain tissue homogenatesderived from infected and uninfected dogs and mice were mixed with mAbs-CnBr agarose resin and washedextensivelly phosphate buffered salin (PBS). Following elution and neutralization, purified proteins weredetected by enzyme-linked immunosirbent assay (ELISA) and Western blotting assay. Of the three mAbs(BB5, AE11 and AF6) as ligands, mAb AE11-CnBr agarose resin yielded highest protein levels as comparedto those of mAb BB5-CnBr agarose and mAb AF6-CnBr agarose resins. In Western Blotting assay, thepurified protein appeared to be 65 Kda (glycoprotein) and 38 kDa proteins. In ELISA test, the purifiedproteins reacted with both mAbs and policlonal antibodies (pAbs).
Kesetaraan Uji Mastitis IPB-1 dengan Metode Breed untuk Mendiagnosis Mastitis Subklinis pada Susu Kerbau Murrah dan Kambing (THE EQUALITY OF IPB-1 MASTITIS TEST WITH BREED METHOD FOR SUB-CLINICAL MASTITIS DETECTION ON MURRAH BUFFALO’S MILK AND GOAT’S MILK Mirnawati Bachrum Sudarwanto; Hera Maheshwari; Faisal Tanjung
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Sub-clinical mastitis causes decrease in milk production and milk quality. It is not only happen tomilking dairies, but also happens to dairy buffalos and goats. The objective of this study is to know thedifferences between IPB-1 mastitis test (IMT) and Breed method to diagnose sub-clinical mastitis ondairy buffaloes and goats. Fourty two samples of buffalo’s milk and 20 samples of goat’s milk were used tosomatic cell count (SCC) with direct and indirect method. Direct method was performed by counting themilk’s SCC with Breed method, and indirect method was performed by observing the reaction betweenIMT reagent and milk. The results showed that 28 from 42 samples (66.67%) of buffalo’s milk and 13 from20 samples (65%) of goat’s milk tested with Breed method came from the herds which suffered from subclinicalmastitis and 27 from 42 samples (64.28%) and 10 from 20 samples (50%) of goat’s milk testedwith IMT showed positive reaction. This research also showed that IMT has sensitivity of 96% and specivicityof 100% for buffalo’s milk and sensitivity of 71% and specivicity of 100% for goat’s milk. IMT can be usedto obtain fast result for sub-clinical mastitis diagnosis and it is faster and easier for buffalo’s and goat’smilk.
Modifikasi Limbah Tulang Sapi Bali dan Pemanfaatannya untuk Adsorpsi Methylene Blue (MODIFICATION OF BALI COW BONE WASTE AND ITS APPLICATION TO ADSORPTION OF METHYLENE BLUE) I Nengah Simpen; Ni Gusti Ayu Made Dwi Adhi Suastuti
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The research of modification, characterization, and application of nano-porous adsorbent base onBali cow bone waste to methylene blue (MB) adsorption has been carried out. The modification was carriedout through extraction of hydroxyapatite (HA) from cow bone, then its activated by 0.4 M NaOH solutionand coating to form active sites of Fe and Fe-Al oxides. The adsorbent was applied to know quantitativelyadsorption capacity coated-HA (CHA) to MB as wastewaters simulation. Compound of CHA wascharacterized amount of active sites by base-acid titration, Bronsted-Lewis acid sites by FTIR spectroscopy,specific surface area by MB method and pore size by surface area analyzer gasorption nitrogen. While,amount of coated-Fe and Al by LIBS. Amount of MB adsorbed was analyzed by UV-Vis spectrophotometer.The results showed that coating of Fe and Fe-Al oxides increased amount of active sites, sites distributionof new Lewis acid site, everage nano pore size with pore volume is microporous (less than 2 nm), and Fe andFe-Al oxides was into HA crystal structure. The amount of active sites was the higest of T1-Fe-Al2O3 andall pores diameter less than 58.182 nm.While, the specific surface area increased T3-Fe2O3>T3-Fe-Al2O3>T2-Fe2O3 respectively. In the adsorption isotherm, coating on HA significantly increased ability of adsorbentand through Freundlich model (R2 > 0.77) with the higest adsorption capacity on T3-Fe2O3 (3.2019 mg/g)then T2-Fe-Al2O3 (2.7921 mg/g). Results of this research indicate that Fe and Fe-Al oxides HA-modifiedincreased adsorption ability.
Penambahan Astaxanthin pada Pengencer Kuning Telur Berbagai Jenis Unggas Dapat Memproteksi Semen Babi Selama Penyimpanan (THE ADDITION OF ASTAXANTHIN ON SPERM DILUENTS PHOSPHATE EGGYOLK OF VARIOUS POULTRY CAN PROTECT QUALITY OF PIG SPERM DURING STORAGE) Wayan Bebas; Wayan Gorda
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was conducted to formulate semen diluent of pigswith a better quality, cheap and easy toprepare using egg yolk of various poultries such as chickens, ducks and quails in combinationwithastaxanthin, a potent antioxidant. The research design used was a completely randomized factorialdesign with three different types of sperm diluents and four levels of astaxanthin concentration. Spermdiluents used were phosphate duck egg yolk, phosphate quail egg yolk and yolks phosphate supplementedrespectivelywith 0,002%, 0,004% and 0,008%astaxanthine. The treated sperm were strored at 5oC for 48hours. The sperm qualities were examined for progressive motility, spermatozoa abnormalities, viabilityand plasma membrane integrity. The result showed that sperm diluents of using duck egg yolk phosphatein combination with 0.002% astaxanthinresulted in the highest progressive motility, viability and plasmamembrane intact while abnormalities spermatozoa is lowest. It can be concluded that phosphate duck eggyolk sperm diluents with the addition of 0,002% astaxanthinappeared to be able to maintain the qualityof pig sperm stored at 5oC for 48 hours.

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