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All Journal Jurnal Penelitian Kesehatan Suara Forikes Jurnal Ilmu dan Teknologi Kesehatan INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) HEALTHY: Jurnal Inovasi Riset Ilmu Kesehatan Journal of Global Pharma Technology Termometer: Jurnal Ilmiah Ilmu Kesehatan Dan Kedokteran Analis Kesehatan Sains (e-Journal) Jurnal Sains Student Research
Krihariyani, Dwi
Poltekkes Kemenkes Surabaya
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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In Silico Analysis of Antiviral Activity and Pharmacokinetic Prediction of Brazilein Sappan Wood (Caesalpinia sappan L.) Against SARS-CoV-2 Spike Glycoproteins Dwi Krihariyani; Edy Haryanto; Retno Sasongkowati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1854

Abstract

Brazilein is one of the secondary sappan wood metabolites which can be used empirically as an antivirus. The SARS-CoV-2 spike (S) glycoproteins play significant roles in attaching and entering the virus into the host cell. This study aims to predict the antiviral activity and pharmacokinetic properties of brazilein of the sappan wood against the in-silico SARS-CoV-2 S glycoproteins with vitamin C as the reference compound. Molegro Virtual Docker 5.5 was used to predict antiviral activity by docking process. SARS-CoV-2 S glycoprotein with NAG ligand available in Protein Data Bank (PDB) (PDB ID: 7C01) was the receptor used. The pkCSM online tool was used to predict the pharmacokinetic properties and toxicity of brazilein. Data were analyzed on the target receptors by comparing the docking bond energies between NAG, brazilein, and vitamin C. The smaller the ligands’ bond energy to the target receptor, the more stable the bonds are. The bond energy of NAG, brazilein, and vitamin C was -59.2864 kcal/mol, -65.8911 kcal/mol, and -53.9093 kcal/mol, respectively. These results suggested that brazilein has a greater capacity as an antivirus compared to NAG and vitamin C. In silico test using the pkCSM online tool demonstrated that brazilein had strong pharmacokinetic properties and relatively low toxicity.
POLA PERTUMBUHAN Staphylococcus aureus PADA MEDIA AGAR DARAH MANUSIA GOLONGAN O, AB, DAN DARAH DOMBA SEBAGAI KONTROL Dwi Krihariyani
Jurnal Ilmu dan Teknologi Kesehatan Vol 3 No 2 (2016): Maret
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.14 KB)

Abstract

Blood Agar Plate (BAP) with sheep blood is the medium used for the identification and isolation of the bacterium Staphylococcus aureus. Because to produce is difficult, but is needed in health education institutions, BAP then used media as a human blood substitute. This study was to observe the pattern of growth of Staphylococcus aureus in media BAP human blood group O, AB and the blood of the sheep as a controle. With the aim to test the feasibility of using human blood group O and AB as essential compounds in the manufacture of a blood substitute sheep BAP media. Staphylococcus aureus growth patterns observed macroscopically to colony morphology, the number of colonies, and the diameter of the haemolysis zone. The suspension of bacteria used werepure cultures of Staphylococcus aureus (ATCC 25923) which is synchronized with Mc Farland 0.5 Standart solution, then do thinning 10-2. The suspension of bacteria that are already experiencing thinning planted in the media BAP human blood group O, AB and sheep blood each at 9 petri dish.From the observation of colony morphology, the number of colonies, and the diameter of the zone of haemolysis performed statistical tests using Kruskal Wallis test values obtained Asymp. Sig of 0.352 for the number of colonies and haemolysis zone diameters greater than α (0.05), which means there is no significant difference between the growth pattern of Staphylococcus aureus in media BAP using human blood group O, AB and sheep blood as a medium BAP controle.
SEROPREVALENSI ANTIBODI IgGToxoplasma gondii PADA IBU DI RANGKAH 6 SURABAYA Dwi Krihariyani Krihariyani; Evy Diah Woelansari; Entuy Kurniawan Kurniawan
Jurnal Ilmu dan Teknologi Kesehatan Vol 3 No 1 (2015): September
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.172 KB)

Abstract

Toxoplasmosis is zoonotic diseases caused Toxoplasma gondii and can infected women. Risk factor Toxoplasmosis infection in women are habits of washing hand after contact wih animal. Thepurpose of the study was to research seroprevalence antibody IgG Toxoplasma gondii among women in Rangkah 6 Surabaya. This research is observational purposive sampling study.Total sample amount 45 sample from women and analyzed antibody IgG Toxoplasma gondii using ELISA technique. The result showed 57,5% (26/40) seropositive antibody IgG Toxoplasma gondii whereas42,5% (14/40) seronegative antibody IgG Toxoplasma gondii. Seropositive 6 of 26 women (23,1%) is pregnant women. Seronegative IgG T.gondii 5 from 14 (35,7%) is pregnant women.These research with Chi-Square test was level value p=0.976that meansno significantrelations between antibody IgG Toxoplasma gondii with habits of washing hand.The prevalence of Toxoplasma gondii antibody among women is high in Rangkah 6 Surabaya. these research will be neededAppropriated to screen women before and during pregnancy, should be dealt in order to minimize of this infection.
STUDI IN SILICO SIFAT FARMAKOKINETIK, TOKSISITAS, DAN AKTIVITAS IMUNOMODULATOR BRAZILEIN KAYU SECANG TERHADAP ENZIM 3-CHYMOTRYPSIN-LIKE CYSTEINE PROTEASE CORONAVIRUS Dwi Krihariyani Dwi; Retno Sasongkowati; Edy Haryanto
Journal of Indonesian Medical Laboratory and Science Vol 1 No 1: Oktober 2020
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v1i1.14

Abstract

The 3-chymotrypsin-like cysteine ??protease (3CLpro) is an enzyme that control replication of the coronavirus life cycle. The 3CLpro is a biological target that will interact with drugs. Brazilein is a major compound found in secang wood that used as an immunomodulator. This study aims to predict the immunomodulatory activity of the brazilein against the 3CLpro using in silico method, with curcumin as comparative compound. In silico test was used to predict immunomodulatory activity by docking using the Molegro Virtual Docke. Receptors used are 3CLpro, PDB code: 6M2N, with ligand 3WL_401[C]. pkCSM online program used to predict pharmacokinetic properties (ADME) and toxicity from brazilein and curcumin. Data was analyzed by comparing the docking bond energy between brazilein, ligands and curcumin at the target receptor. The lower the ligand bond energy, the more stable the bond is formed. The results of the in silico test showed that the ligand bond energy = -83,1153kcal/mol, brazilein = -82.0583kcal/mol, and curcumin = -115.852kcal/mol. Brazilein has lower potential as an immunomodulator than ligands and curcumin. The results of the in silico test using the pkCSM online program showed that the brazilein compound had good pharmacokinetic properties and low toxicity.
Potential of Quercetin in Bitter Melon (Momordica charantia L) as an Antidiabetic: In Silico Study Roni Yuliwar; I Ketut Sudiana; Kusnanto Kusnanto; Dwi Krihariyanie; Hidayat Arifin
Journal of Global Pharma Technology Volume 12 Issue 11 (2020) Nov. 2020
Publisher : Journal of Global Pharma Technology

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Abstract

Purpose: The purpose of this study is to predict the in silico molecular interactions of bitter melon quercetin with GLP-1 receptors using the comparable compounds of myricetin and allosteric modulators. Methods: The in silico test was used to predict the quercetin molecular interactions by docking using the Molegro Virtual Docker computer program. The receptor used was human GLP-1, code PDB: 5VEX with allosteric modulators ligand (97V_1201 [A]). As part from predicting molecular interactions, this study also carried out the prediction of its pharmacokinetic properties (ADME) and the toxicity of quercetin and myricetin using the pkCSM online tool program. The data analysis was performed by comparing the docking bond energies between quercetin, the allosteric modulator ligands and the myricetin comparators at the target receptor. The lower the bond energy of the ligands to the target receptor, the more stable the bonds are. This can be used to predict the biological activity of the compound. Results: The in silico test results showed that the bond energy of quercetin = -70.2678 kcal/mol, myricetin = -105,298 kcal/mol and allosteric modulators = -126,992 kcal/mol. Conclusions: The above test results indicate that quercetin has antidiabetic potential by activating the GLP-1 receptor although it is lower than the myricetin and allosteric modulators. The results of the in silico test using the pkCSM online tool program showed that the quercetin compound had good pharmacokinetic properties and a low toxicity level.
The Pola Pertumbuhan Bakteri Staphylococcus aureus Pada Media Blood Agar Plate (BAP) Menggunakan Darah Manusia, Darah Ayam, dan Darah Domba Krihariyani, Dwi
Analis Kesehatan Sains Vol. 13 No. 1 (2024): Analis Kesehatan Sains
Publisher : Politeknik Kesehatan Kementerian Kesehatan Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/anakes.v13i1.93

Abstract

ABSTRACT Transmission of infection from Staphylococcus aureus bacteria generally occurs in people who have low awareness of maintaining body hygiene, starting with the bacteria entering the skin through scratches and then multiplying causing inflammation. Blood Agar Plate (BAP) is a general medium that is used as a special growth medium to identify certain types of bacteria using the addition of sheep's blood. The aim of this research was to find the growth pattern of Staphylococcus aureus bacteria on Blood Agar Plate (BAP) media which used human blood, chicken blood and sheep blood. Add 5% chicken blood, human blood and sheep blood to the Blood Agar Plate (BAP) then plant Staphylococcus aureus and observe for 24 hours followed by 48 hours. In the 24 hour sample with a total of 27 samples, the results obtained for the number of colonies in sheep blood were 246 x 10-12 CFU/mL, in human blood it was 198.5 x 10-12 CFU/mL, in chicken blood the TBUD results were obtained (Cannot be calculated). In the 48 hour sample with a total of 27 samples, the results obtained for the number of sheep blood colonies were 224.5 x 10-12 CFU/mL, for human blood it was 203 x 10-12 CFU/mL, for chicken blood the results were TBUD (Cannot to be calculated). The macroscopic results are round in shape, grayish white in color, and have a zone of complete hemolysis. Keywords: Staphylococcus aureus, Blood Agar Plate, blood ABSTRAK Penularan infeksi dari bakteri Staphylococcus aureus umumnya terjadi pada masyarakat yang memiliki kesadaran rendah dalam menjaga kebersihan tubuh, diawali dengan masuknya bakteri ke kulit melalui goresan luka kemudian berkembang biak menyebabkan peradangan. Blood Agar Plate (BAP) adalah media umum yang digunakan sebagai media pertumbuhan khusus untuk mengidentifikasi jenis bakteri tertentu menggunakan penambahan darah domba. Tujuan pada penelitian ini untuk mengetahui pola pertumbuhan bakteri Staphylococcus aureus pada media Blood Agar Plate (BAP) yang menggunakan darah manusia, darah ayam, dan darah domba. Ditambahkan sebanyak 5% darah ayam, darah manusia, dan darah domba pada Blood Agar Plate (BAP) lalu ditanam Staphylococcus aureus dan dilakukan pengamatan dalam waktu 24 jam dilanjutkan pada 48 jam. Pada sampel 24 jam dengan total 27 sampel, maka diperoleh hasil pada jumlah koloni pada darah domba sebanyak 246 x 10-12 CFU/mL, pada darah manusia sebanyak 198,5 x 10-12 CFU/mL, pada darah ayam didapatkan hasil TBUD (Tidak Bisa Untuk Dihitung). Pada sampel 48 jam dengan total 27 sampel, diperoleh hasil pada jumlah koloni darah domba sebanyak 224,5 x 10-12 CFU/mL, pada darah manusia sebanyak 203 x 10-12 CFU/mL, pada darah ayam didapatkan hasil TBUD (Tidak Bisa Untuk Dihitung). Hasil makroskopis yaitu berbentuk bulat, berwarna putih keabuan, serta zona hemolisis sempurna. Kata Kunci : Staphylococcus aureus, Blood Agar Plate, darah
Uji Efektivitas Daya Hambat Ekstrak dan Perasan Lidah Buaya (Aloe barbadensis Miller) pada Bakteri Staphylococcus Aureus dengan Metode Difusi Farah Adilah Ambar Sari; Suliati Suliati; Syamsul Arifin; Dwi Krihariyani
Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran Vol. 2 No. 4 (2024): Oktober : Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran
Publisher : Pusat Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/termometer.v2i4.4307

Abstract

Aloe vera is a plant that is rich in minerals, vitamins, and antibacterial properties. A study to analyze the antibacterial effectiveness of aloe vera in inhibiting the growth of Staphylococcus aureus bacteria isolated from acne pus. This study was an experimental laboratory study using the disc diffusion method, located at the Bacteriology Laboratory, Department of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya, from February to May 2024. There were 6 sample treatments with 4 replications, 4 treatments of extract and juice concentration variations, namely concentrations of 70%, 80%, 90%, 100%, positive control using penicillin antibiotics, and negative control using clean distilled water. The results of this study show that at aloe vera extract concentrations of 70%, 80%, 90%, and 100%, the average diameter of the inhibition zone was 9 mm, 10 mm, 11 mm, and 12 mm, respectively, while in aloe vera juice, these concentrations did not form an inhibition zone. So it was concluded that aloe vera extract is more effective than aloe vera juice, the most successful test material to slow the growth of Staphylococcus aureus bacteria in this study was aloe vera extract with a concentration of 100%. This study also conducted qualitative phytochemical testing on aloe vera extract which has antibacterial compounds, namely saponins, tannins, flavonoids, and terpenoids. The results of the study on the effectiveness of the inhibitory power of aloe vera extract and juice against Staphylococcus aureus bacteria using the disc diffusion method were analyzed using quantitative descriptive and presented in tabulation form in the form of the diameter of the inhibition zone formed.
Pour Plate dan Spread Plate Sebagai Metode yang Akurat dalam Pemeriksaan Jumlah Bakteri dalam Urin pada Suhu Ruang dan Suhu Kulkas A'yunin, Zahratin Nisa'; Pestariati, Pestariati; Endarini, Lully Hanni; Krihariyani, Dwi
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 16, No 2 (2025): April-Juni 2025 (up coming)
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf.v16i1.5012

Abstract

in process
KORELASI LEUKOSIT DAN IGM ANTI-SALMONELLA SEBAGAI PENUNJANG DIAGNOSIS PASIEN SUSPEK DEMAM TIFOID Ariyani, Ahna Fitri; Krihariyani, Dwi; Istanto, Wisnu; Suhariyadi, Suhariyadi
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 4 No. 2 (2025)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v4i2.5826

Abstract

ABSTRACT Typhoid fever is an infectious disease that remains prevalent in Indonesia and often presents with non-specific clinical symptoms, necessitating supportive diagnostic examinations. Two commonly used parameters are leukocyte count and anti-Salmonella typhi IgM serological testing using the rapid test method. This study aims to determine the correlation between leukocyte count and the results of the anti-Salmonella IgM rapid test in patients suspected of having typhoid fever as a diagnostic aid. This research employed an analytical observational method with a cross-sectional approach. A total of 32 out of 45 suspected typhoid fever patients were selected through purposive sampling at RSUD Dr. Sayidiman Magetan. Leukocyte counts were measured using an automated hematology analyzer, and IgM testing was performed using the immunochromatographic rapid test method. The results showed that 59.4% of patients had leukopenia, and only 21.9% tested positive for IgM. Spearman’s rank correlation test yielded a significance value (p) = 0.027 and a correlation coefficient (r) = 0.391, indicating a moderate and statistically significant correlation between the two variables. In conclusion, there is a significant positive correlation between leukocyte count and anti-Salmonella IgM results, suggesting that both tests can serve as complementary diagnostic tools for the early diagnosis of typhoid fever. ABSTRAK Demam tifoid merupakan penyakit infeksi yang masih sering dijumpai di Indonesia dan menimbulkan gejala klinis yang tidak spesifik, sehingga diperlukan pemeriksaan penunjang untuk membantu diagnosis. Dua parameter yang umum digunakan adalah jumlah leukosit dan uji serologis IgM anti-Salmonella typhi dengan metode rapid test. Penelitian ini bertujuan untuk mengetahui korelasi antara jumlah leukosit dan hasil rapid test IgM anti-Salmonella pada pasien suspek demam tifoid sebagai penunjang diagnosis. Penelitian ini menggunakan metode observasional analitik dengan pendekatan cross-sectional. Sampel sebanyak 32 pasien dari 45 pasien suspek demam tifoid yang diambil di RSUD Dr. Sayidiman Magetan secara purposive sampling. Pemeriksaan jumlah leukosit dilakukan dengan alat hematologi otomatis dan uji IgM dilakukan menggunakan metode rapid test imunokromatografi. Hasil penelitian menunjukkan bahwa 59,4% pasien mengalami leukopenia dan hanya 21,9% pasien yang menunjukkan hasil IgM positif. Uji korelasi Rank Spearman menunjukkan nilai signifikansi (p) = 0,027 dan koefisien korelasi (r) = 0,391, yang menunjukkan adanya hubungan yang cukup kuat dan signifikan secara statistik antara kedua variabel. Kesimpulannya, terdapat hubungan positif yang signifikan antara jumlah leukosit dan hasil IgM anti-Salmonella, sehingga keduanya dapat digunakan sebagai pemeriksaan penunjang yang saling melengkapi dalam diagnosis awal demam tifoid.
Korelasi Kadar Serum Iron Dengan Hemoglobin Pada Pasien Gagal Ginjal Kronik Level V Di RSUD Dr. Harjono S Ponorogo Yulia Hermawati; Dwi Krihariyani; Syamsul Arifin; Wisnu Istanto
JOURNAL SAINS STUDENT RESEARCH Vol. 3 No. 4 (2025): Jurnal Sains Student Research (JSSR) Agustus
Publisher : CV. KAMPUS AKADEMIK PUBLISING

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61722/jssr.v3i4.5975

Abstract

Chronic Kidney Disease (CKD) is not merely a condition of permanently decreased renal function, but it also significantly impacts patients’ quality of life. One of the major complications is anemia. Iron deficiency is a primary contributor to anemia in CKD patients, often resulting from reduced erythropoietin production, blood loss during hemodialysis, and inadequate iron intake. This study aims to determine the relationship between serum iron levels (iron in the blood) and hemoglobin levels in stage V chronic kidney disease (CKD) patients undergoing regular hemodialysis at Dr. Harjono S. Ponorogo Regional Hospital, using a quantitative descriptive-analytic approach based on secondary data from the hospital's Laboratory Information System (LIS). A total of 24 CKD patients undergoing hemodialysis twice a week during the period of July to December 2024 were included as research subjects. The results showed that all patients experienced anemia. Although most patients had serum iron levels within the normal range, hemoglobin levels remained low. Statistical analysis revealed a moderate correlation between serum iron and hemoglobin levels, with a statistically significant p-value (p < 0.05) in both examinations. A moderate and statistically significant correlation was found between serum iron and hemoglobin levels in stage V CKD patients, indicating that monitoring iron status is important in the management of anemia in chronic kidney disease.
Co-Authors ., Pestariati A'yunin, Zahratin Nisa' Arifin, Hidayat Ariyani, Ahna Fitri Edy Haryanto Endarini, Lully Hanni Entuy Kurniawan Kurniawan Evy Diah Woelansari Farah Adilah Ambar Sari Istanto, Wisnu Kusnanto Kusnanto Reni Susanti Retno Sasongkowati Retno Sasongkowati Roni Yuliwar S.Pd. M Kes I Ketut Sudiana . Sholihah, Irodatus Suhariyadi Suhariyadi Suliati Suliati Syamsul Arifin Syamsul Arifin Wisnu Istanto Yulia Hermawati