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SNPs in splicing region and miRNA binding region of Bos taurus TREM-1 gene reveals its association with mastitis N. S. Pandupuspitasari; F. A. Khan; A. Setiaji; D. A. Lestari; Z. Shujun
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 4 (2023): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.4.306-314

Proper splicing is important for the functioning of a gene, and any interruption in splicing causes several deleterious events. Triggering receptors present on myeloid cells, TREM-1, are implicated in inflammation and act as amplifiers by mediating the release of proinflammatory chemokines and cytokines in response to fungal and bacterial infections. In bovines, mastitis is an inflammatory disease in which mammary gland inflammation is generally caused by bacteria. We found rs109937179 and rs208224995 SNPs in the splicing and miRNA binding region of TREM-1 gene in Chinese Holstein cows. The genotype distribution of the alleles for TREM-1 (rs109937179 and rs208224995) gene polymorphisms was investigated in 364 and 320 Chinese Holstein cows, respectively. We found that the GG genotype of the rs109937179 polymorphism and rs208224995 genotype of CA within the TREM-1 gene were associated with an increased risk of mastitis. Importantly, rs109937179 was found in the splicing region of TREM-1, and rs208224995 has a miRNA binding region for bta-miR- 2329-3p in the 3'UTR, which determines its effective roles in gene expression regulation.

Status Hematology of Nellore Crossbreed through Feed Supplementation Containing Gopro, Rumen-Protected Nutrients, and Fecal Microbiota Transplantation Ardiansyah, Prasetyo; Widiyanto, Widiyanto; Pandupuspitasari, Nuruliarizki Shinta; Prasetiyono, Bambang Waluyo Hadi Eko
ANIMAL PRODUCTION Vol. 26 No. 2 (2024)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2024.26.2.297

Produksi daging yang optimal dapat terjadi pada ternak yang sehat. Pada hewan ruminansia, produksi daging dapat dioptimalkan dengan meningkatkan perkembangbiakan mikroba rumen, menggunakan nutrisi non-degradable rumen, dan menjaga keseimbangan mikroba melalui penerapan Gopro (produk eksklusif yang dikembangkan di laboratorium kami), nutrisi non-degradable rumen (RUN), dan transplantasi mikrobiota tinja (FMT). kami kemudian menentukan profil hematologi sapi yang diberi pakan Gopro, RUN, dan FMT. Lima belas ekor sapi peranakan Nellore berumur kurang lebih 8 bulan dengan rata-rata bobot badan 199,53 ± 22,82 kg, dibagi menjadi tiga kelompok pakan: T1 mendapat pakan yang terdiri dari 40% hijauan dan 60% konsentrat yang dilengkapi dengan Gopro; T2 mendapat diet yang sama dengan suplementasi RUN; dan T3 menerima diet T2 dengan 5 g FMT. Pakan diberikan sebanyak 3% bahan kering/berat badan, dengan akses air minum ad libitum. Nilai hematologi masih dalam batas nilai hematologi normal. Kami mengamati nilai hemoglobin 11,78-12 gr/dL; hematokrit 32,7-34,52 %; leukosit 9,75-14,25 10 3 /μL; neutrofil 1,93-2,54 10 3 /μL, eosinofil 0,32-0,48 10 3 /μL, limfosit 7,38-10,12 10 3 /μL. Kombinasi Gopro dan RUN mampu mengurangi zat penyebab alergi. Penggunaan FMT tidak menimbulkan efek samping, seperti alergi dan infeksi parasit. Pemberian kombinasi Gopro, RUN dan FMT mampu menjaga kesehatan.

Status Hematology of Nellore Crossbreed through Feed Supplementation Containing Gopro, Rumen-Protected Nutrients, and Fecal Microbiota Transplantation Ardiansyah, Prasetyo; Widiyanto, Widiyanto; Pandupuspitasari, Nuruliarizki Shinta; Prasetiyono, Bambang Waluyo Hadi Eko
ANIMAL PRODUCTION Vol. 26 No. 2 (2024)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2024.26.2.297

Produksi daging yang optimal dapat terjadi pada ternak yang sehat. Pada hewan ruminansia, produksi daging dapat dioptimalkan dengan meningkatkan perkembangbiakan mikroba rumen, menggunakan nutrisi non-degradable rumen, dan menjaga keseimbangan mikroba melalui penerapan Gopro (produk eksklusif yang dikembangkan di laboratorium kami), nutrisi non-degradable rumen (RUN), dan transplantasi mikrobiota tinja (FMT). kami kemudian menentukan profil hematologi sapi yang diberi pakan Gopro, RUN, dan FMT. Lima belas ekor sapi peranakan Nellore berumur kurang lebih 8 bulan dengan rata-rata bobot badan 199,53 ± 22,82 kg, dibagi menjadi tiga kelompok pakan: T1 mendapat pakan yang terdiri dari 40% hijauan dan 60% konsentrat yang dilengkapi dengan Gopro; T2 mendapat diet yang sama dengan suplementasi RUN; dan T3 menerima diet T2 dengan 5 g FMT. Pakan diberikan sebanyak 3% bahan kering/berat badan, dengan akses air minum ad libitum. Nilai hematologi masih dalam batas nilai hematologi normal. Kami mengamati nilai hemoglobin 11,78-12 gr/dL; hematokrit 32,7-34,52 %; leukosit 9,75-14,25 10 3 /μL; neutrofil 1,93-2,54 10 3 /μL, eosinofil 0,32-0,48 10 3 /μL, limfosit 7,38-10,12 10 3 /μL. Kombinasi Gopro dan RUN mampu mengurangi zat penyebab alergi. Penggunaan FMT tidak menimbulkan efek samping, seperti alergi dan infeksi parasit. Pemberian kombinasi Gopro, RUN dan FMT mampu menjaga kesehatan.

Correction to: Supplementation of Zinc Palm Oil Soap Improves Feed Fermentability and Unsaturated Fatty Acid Profile in Rumen Liquid Muktiani, A.; Widiyanto, W.; Pandupuspitasari, N. S.
Tropical Animal Science Journal Vol. 48 No. 3 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.3.279

Correction to: Tropical Animal Science Journal, 47(3), 371-380. https://doi.org/10.5398/tasj.2024.47.3.371 An error occurred on Page 373, Table 1, on “Note” under the table. The previous version was written: 1Total digestible nutrients (TDN) were calculated using TDN (%DM). TDN = -17.2649 + 1.2120(PK) + 0.8352(BETN) + 2.4637(LK) + 0.4475(SK), according to Wardeh (1981). The correct version should be: 1Total digestible nutrients (TDN) were calculated using TDN (%DM). TDN = -17.2649 + 1.2120(CP) + 0.8352(NFE) + 2.4637(EE) + 0.4475(CF), according to Wardeh (1981).

Tingkah Laku Makan Kambing Perah dengan Subtitusi Daun Kayu Jawa (Lannea coromendelica) dalam Pakan Armayanti, Andi Kurnia; Irfan, Andi Muh.; Hermawansyah; Joelal Achmadi; Nusantara, Limbang Kustiawan; Pandupuspitasari, Nuruliarizki Shinta; Danial
Jurnal Peternakan Lokal Vol. 7 No. 2 (2025): Jurnal Peternakan Lokal
Publisher : Program Studi Peternakan Universitas Muslim Maros

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46918/peternakan.v7i2.2804

Keterbatasan dan ketersediaan pakan menjadi faktor penghambat dalam proses pemeliharaan ternak kambing perah. Pemanfaatan daun kayu jawa (Lannea coromendelica) menjadi pakan alternatif jika dilihat dari kandungan nutriennya. Penelitian ini bertujuan untuk mengetahui pengaruh substitusi tepung daun kayu jawa (Lannea coromandelica) terhadap tingkah laku makan kambing perah. Substitusi pakan konvensional dengan daun kayu jawa dilakukan untuk mengevaluasi efektivitasnya dalam mendukung perilaku makan, ruminasi, dan istirahat ternak. Penelitian dilaksanakan di CV. Zafira Goat Farm, Jeneponto, menggunakan Rancangan Acak Kelompok (RAK) dengan empat perlakuan (P₀ = 0% daun kayu jawa, P₁ = 20%, P₂ = 40%, P₃ = 60%) dan lima ulangan. Parameter yang diamati meliputi durasi makan, ruminasi, dan istirahat, dicatat se’tiap 15 menit dari pukul 08.00–16.00 WITA. Hasil penelitian menunjukkan bahwa substitusi tepung daun kayu jawa tidak memberikan pengaruh yang nyata (P>0,05) terhadap tingkah laku makan, ruminasi, maupun istirahat kambing perah. Meskipun demikian, pada beberapa jam tertentu terjadi perbedaan aktivitas yang mencerminkan adaptasi ternak terhadap komposisi pakan yang berbeda. Daun kayu jawa tetap menunjukkan potensi sebagai sumber pakan alternatif karena mengandung protein kasar dan serat yang mendukung kebutuhan fisiologis kambing perah tanpa mengganggu perilaku alaminya.

Supplementation of Zinc Palm Oil Soap Improves Feed Fermentability and Unsaturated Fatty Acid Profile in Rumen Liquid Muktiani, A.; Widiyanto, W.; Pandupuspitasari, N. S.
Tropical Animal Science Journal Vol. 47 No. 3 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.3.371

This study aimed to evaluate the effects of energy and organic zinc supplements, specifically zinc palm oil soap (ZPOS), on digestibility and unsaturated fatty acid profiles in vitro. The study used a completely randomized design with 4 treatments and 5 replications. The treatments were: T0= basal diet without supplementation, T1= basal diet + 5% palm oil (PO), T2= basal diet + 5% partial ZPOS (3.75% ZPOS + 1.25% PO), and T3= basal diet + 5% ZPOS. The inoculum source was rumen liquid from three fistulated female dairy goats and was homogenized. The goats were fed ration consisting of corn straw, soybean hulls, and concentrate containing total digestible nutrients (TDN) 63%, crude protein (CP) 14%, and neutral detergent fiber (NDF) 35%. Results showed that both 5% partial ZPOS and 5% ZPOS supplementation (T2 and T3) resulted in the increase of total volatile fatty acids (VFA), acetate, propionate, butyrate, unsaturated fatty acids (USFA) and a decrease in the ratio of acetate/propionate (A/P) compared to the control and supplementation of 5% PO (p<0.05). Supplementation of 5% partial ZPOS (T2) is better than 5% ZPOS because increased the digestibility of ether extract (EE), crude fiber (CF), NDF, and acids detergent fiber (ADF) (p<0.05) and decreased of methane compared to the control (p<0.05). In conclusion, adding 5% partial ZPOS (3.5% ZPOS and 1.5% PO) increases fiber digestibility, VFA, LCFA, and USFA concentration, and decreases methane production in the rumen liquid.

PCA-based metagenomics reveals functional and taxonomic diversity in Kedu chicken gut microbiota NURULIARIZKI SHINTA PANDUPUSPITASARI; SUGIHARTO SUGIHARTO; IKANIA AGUSETYANINGSIH; DELA AYU LESTARI; FAHEEM AHMED KHAN; ASEP SETIAJI; MUHAMMAD ASIF RAZA
Biodiversitas Journal of Biological Diversity Vol. 26 No. 8 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260832

Abstract. Pandupuspitasari NS, Sugiharto S, Agusetyaningsih I, Lestari DA, Khan FA, Setiaji A, Raza MA. 2025. PCA-based metagenomics reveals functional and taxonomic diversity in Kedu chicken gut microbiota. Biodiversitas 26: 4035-4041. The Gastrointestinal Tract (GIT) microbiota plays a central role in poultry health, productivity, and resilience, influencing digestion, nutrient absorption, immune development, and disease resistance. This study provides the first multi-database Principal Component Analysis (PCA)-based metagenomic characterization of the gut microbiota in Kedu chickens (Gallus gallus (Linnaeus, 1758)), an indigenous Indonesian breed. Digesta from five GIT segments (crop, gizzard, jejunum, colon, and cecum) of 21 traditionally reared Kedu chickens were sequenced on the Illumina NovaSeq X Plus platform, yielding 1,523,876 unigenes. Functional annotation against Non-Redundant Protein Database (NR), Clusters of Orthologous Groups (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG), Carbohydrate-Active enZYmes Database (CAZy), Antibiotic Resistance Genes Database (ARDB), Comprehensive Antibiotic Resistance Database (CARD), and Virulence Factor Database (VFDB) revealed enrichment of genes involved in metabolism (38.2%), cellular processes (15.7%), environmental information processing (12.6%), and genetic information processing (11.0%). PCA separated GIT segments by functional specialization: The jejunum clustered distinctly (PC1 variance = 26.48% for NR, 30.04% for COG, 67.16% for KEGG) due to nutrient absorption-related functions, while the cecum exhibited the highest diversity and carbohydrate-active enzyme profiles (PC1 = 24.98% for CAZy). ARDB/CARD analysis showed that distal GIT segments (colon, cecum) contained higher densities of antimicrobial resistance genes, and VFDB revealed elevated virulence factor abundance in ileum and cecum. These findings highlight segment-specific microbiota roles, supporting the development of targeted probiotics and feed strategies, and contribute to conserving the functional biodiversity of Kedu chickens in sustainable production systems.

Effect of faecal microbiota transplantation on growth, haematological and intestinal indices of Kedu chickens M. R. Yousaf; A. N. M. Ramli; D. A. Lestari; S. Sutaryo; I. Agusetyaningsih; T. Ayasan; N. S. Pandupuspitasari; A. Setiaji; S. Sugiharto
Journal of the Indonesian Tropical Animal Agriculture Vol 51, No 3 (2026): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.51.3.162-170

The study examined the effect of lyophilized fecal microbiota transplantation (FMT) from healthy chicken donors on growth performance, haematological indices and intestinal morphology of Kedu chickens during the starter phase. A number of 120 Kedu chickens (0-6 weeks old) were randomly as-signed to T0 (orally inoculated with 1 mL PBS), T1 (inoculated with 0.5 mL FMT) and T2 (1.0 mL FMT). Body weight and feed intake were weekly recorded, while blood and intestinal segments were collected at week 6. Results showed that live body weight and body weight gain were highest (p<0.001) in T1, and the lowest in T0 group. Feed conversion ratio (FCR) was better (p=0.001) in T1 and T2 compared to T0 group. Feed intake was lower (p=0.014) in T2 than in T0 and T1 groups. The FMT administration tended to increase erythrocytes (p=0.054) and haemoglobin levels (p=0.056) of Kedu chickens. The relative weight of duodenum, jejunum, ileum, caecum and colon were lower (p<0.05) in T1 and T2 compared to those in T0 group. The FMT administration had no effect (p>0.05) on duodenal and jejunal villi height, crypt depth and the ratio of villi height to crypt depth of Kedu chickens. In conclusion, daily low-dose lyophilized FMT was associated with improved growth perfor-mance and feed efficiency in starter Kedu chickens, while haematological and intestinal morphological responses were limited.

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