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All Journal Indonesian Journal of Human Nutrition Indonesian Journal of Obstetrics & Gynecology Science Amerta Nutrition Andalas obstetrics and gynecology journal JOURNAL LA MEDIHEALTICO Jurnal Riset Kebidanan Indonesia
Utama, Bobby Indra
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary Other

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ISOLATION OF AMNIOTIC FLUID MESENCHYMAL STEM CELLS (AF MSCs) OBTAINED FROM CAESAREAN SECTIONS Utama, Bobby Indra
Andalas Obstetrics And Gynecology Journal Vol. 2 No. 1 (2018)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.2.1.1-9.2018

Abstract

Amniotic fluid is a liquid that fills the amniotic cavity which has defense and nutritional functions in fetal development. Human aterm amniotic fluid can be an ideal alternative as a source of mesenchymal stem cells, originating from the neonate. Preclinical studies of second and third trimester amnion fluid cells confirmed the number of potential donors from this wasted material. In several studies, AF-MSCs express mesenchymal markers such as CD90, CD73 (SH3, SH), CD105 (SH2), CD29, CD166, CD49e, CD58 and CD44 (MHC class I). These cells also express HLA-ABC antigens, CD 34, CD 45 which are hematopoietic markers, and endothelial CD31 markers. There is no expression of CD10, CD11b, CD14, CD34, CD117, EMA and HLA-DR, DP, DQ antigens. Most of AF-MSCs have pluripotent properties which are characterized by the discovery of octamer binding protein 3/4 (Oct-3/4), transcription factors Nanog (Nanog), and stage-specific embryonic antigen 4 (SSEA-4) on RT-PCR examination. From this study, 8 million cells was isolated. These cells will be used for research on pelvic organ prolapse therapy by using AF-MSCs. AF-MSCs isolation totally takes 6 weeks. From 1 flask, 2 million of stem cells was obtained. Keywords: amniotic fluid, AF-MSCs
UTERUS BIKORNU Marni, Herti; Ferry, Ferdinal; Utama, Bobby Indra
Andalas Obstetrics And Gynecology Journal Vol. 2 No. 2 (2018)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.2.2.115-118.2018

Abstract

Background : Uterus bicornis subseptum is a deformity of the uterus such as the shape of the heart, the fundus appears to be curved inward, and has a barrier or wall inside to the uterine cavity. This abnormality occurs due to failure of the fusion of the left and right mullerian ducts. If pregnant, women who have this form of the uterus will usually experience a location abnormality, in which the fetus is often transverse or breech. However, women who have this disorder sometimes find it difficult to get pregnant so they experience infertility. In addition, there is usually a disturbance in the formation of the kidneys, but in this patient there was no abnormality in the urinary tract. In cases where the patient does not have children and a bicornu uterine abnormality is found, uterine repair is performed and it is hoped that with the uterine anatomy returning to normal, the patient can become pregnant.Objective : Discuss the management of cases of congenital abnormalities of the internal genital organs in patients with a bicornu uterus.Method : Case Report.Case : Reported a patient aged 25 years with primary infertility 4 years and a bicornu uterus. In patients, chromotubation was performed first, after chromotubation, the patient was performed metrosplasty to remove the bulkhead in the uterus and to repair the uterus. During the post-op follow-up at the hospital the patient did not show any signs of infection or acute abdomen, then the patient was discharged on day 5. The patient was advised to go to the OBGYN polyclinic regularly for further follow-up to the success of his uterine repair. Assessment can be done in several ways including transvaginal ultrasound to assess whether there is adhesions to the endometrium from the results of uterine repair, HSG, SIS or hysteroscopy can also be done in assessing the success of metroplasty in this patient.Conclusion : After metrosplasty in a patient with a bicornu uterus, further follow-up is required to determine the success of this procedure by performing a follow-up ultrasound.Keywords: Bicornu uterus, metroplasty
SEPTUM VAGINA TRANSVERSA Utama, Bobby Indra; Ermawati, Ermawati
Andalas Obstetrics And Gynecology Journal Vol. 1 No. 2 (2017)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.1.2.80-85.2017

Abstract

The vagina is a membranous muscular tube that connects the vulva and uterus. Congenital or congenital abnormalities in the form of complete or partial absence of the vagina (vaginal agenesis). Patients who experience vaginal agenesis have less frequency, namely 1 in 4000 births, 1 in 4000 to 10,000 births (ACOG). Meanwhile in Dr. Cipto Mangunkusumo Jakarta from 1995 to 1999, an average of 10-12 cases per year. It has been reported the case of a female patient aged 14 years who entered the Gynecology Ward Dr. M. Djamil Padang on January 25, 2014 at 11.00 WIB with a delivery from the RSMJ gyn clinic with a diagnosis of transverse vaginal septum + hematometra + hematotrachelos + hematokolpos planned for a septal incision. After performing the operative action, ± 500 cc of blackish brown liquid was successfully removed.Keywords: Agenesis Vagina, TVS, Tranverse Septum Vagina, Case Report
PERBEDAAN LEVEL RERATA KEKUATAN OTOT DASAR PANGGUL SEBELUM DAN SETELAH PERSALINAN SPONTAN PADA KELOMPOK INKONTINENSIA URIN DAN KELOMPOK NORMAL Utama, Bobby Indra; Sari, Hasni Kemala; Bachtiar, Hafni
Andalas Obstetrics And Gynecology Journal Vol. 1 No. 1 (2017)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.1.1.7-14.2017

Abstract

Trauma to the pelvic floor during delivery is now recognized as a major etiological factor against PFM disorders such as urinary incontinence, pelvic organ prolapse and fecal incontinence. This study was conducted to analyze the differences in mean levels of differences of pelvic muscle strength before and after spontanous labor between stress urinary inconti-nence group and normal group. This research was done using analytic method with cross sectional design in 13 women with stress urinary incontinence, and 17 women with normal group. Subjects were collected in hospitals of Pariaman, Padang from May to December 2014. Examination of the pelvic floor muscle strength was performed with a perineometer. Differences between the mean difference in the strength of the pelvic floor muscles before and after spontaneous delivery between the two groups were analyzed using independent t test. The mean difference between the strength of the pelvic floor muscles before and after spontaneous labor in stress urinary incontinence group was larger than normal group (3.85 + 1.281 cmH2O vs 2,00 + 1.173 cmH2O, p = 0.000). The mean difference between the strength of the pelvic floor muscles before and after spontaneous labor in stress urinary incontinence group was significantly greater than the normal group.Keywords: Pelvic Floor Muscle, Urinary Incontinence, Spontaneous Labor
Co-Authors Andani Eka Putra Arni Amir Berliani, Hasvia Burhan, Ida Rahmah Desmawati Desmawati Dessy Arisanty Diniy Miftahul Muthmainah Ermawati Ermawati Ferry, Ferdinal Firdawati, Firdawati Hafni Bachtiar Haviz Yuad Hindun Mila Hudzaifah Husna Yetti Laila Rahmi, Laila Marni, Herti Nelmi Silvia Nur Indrawaty Lipoeto Rahman, Andio Roby Putrama Rossa, Fayola Sari, Hasni Kemala Sari, Nur Purnama Sari, Yulia Margaretta Sihaloho, Febri Theresia Ulfa Farrah Lisa, Ulfa Farrah Vella Amalia