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All Journal HAYATI Journal of Biosciences Jurnal Hortikultura Indonesia (JHI) AL KAUNIYAH Jurnal Penelitian Tanaman Industri Indonesian Journal of Biotechnology Jurnal Sumberdaya Hayati JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Journal of Microbial Systematics and Biotechnology Jurnal Penelitian Tanaman Industri (Littri) Berkala Penelitian Hayati Makara Journal of Science Annales Bogorienses
Aris Tjahjoleksono
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor. Jl. Agathis, Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Optimization of Somatic Embryogenesis Induction of Cassava (Manihot esculenta Crantz) Idha Susanti; Suharsono Suharsono; Utut Widyastuti; Ulfah Juniarti Siregar; Aris Tjahjoleksono
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (535.56 KB) | DOI: 10.14203/ann.bogor.2017.v21.n2.45-51

Abstract

The embryogenesis (SE) has important role for genetic engineering of cassava (Manihot esculenta Crantz). However, the success of SE induction depend on plant growth regulator s (PGR)s and treatment enriched in induction media.  This experiment tried to induce callus formation of cassava from several in vitro explants: immature leaf, apical bud, and internode; and to develop somatic embryogenesis of cassava in several media enriched with tyrosine and copper sulphate (CuSO4) added into media enrich with picloram as treatment.  Different response of explants source to callus induction treatment from those three varieties in callus induction as well as friable callus formation were found in this experiment. The best medium to induce varied with variety; MS media supplemented 12 mg/L picloram + 0.5 mg/L CuSO4 was the best for “Adira 4” and  half MS and half GD media supplemented 12 mg/L picloram + 100 mg/L tyrosine for “Malang 6”.  All treatments resulted somatic embryo which developed indirectly and in morphologically normal somatic embryos
Characterization and application of nitrogen-fixing and indole-3-acetic acid producing bacteria A13 in Oil Palm (Elaeisguineensis Jacq.) seedling Ismi Isti'anah; Nisa Rachmania Mubarik; Aris Tjahjoleksono
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.77

Abstract

Oil palm plantations have a good prospect in Indonesia. One of the efforts to improve the productivity of oil palm plantation is the application of bacteria as biological fertilizer. The research was conducted to characterize and apply the nitrogen-fixing and indole-3-acetic acid producing bacteria in oil palm seedlings. The bacteria was isolated from soil samples which taken from Taman Nasional Bukit Dua Belas (TNBD) Jambi. Nitrogen free bromthymol blue (NFB) is used as media for nitrogen-fixing bacterial isolation. Selected isolate named A13 had an ability to form white pellicle on the surface of the semisolid medium, increased the pH, and changed the color of medium from green to blue Isolate A13 was identified as Gram-negative bacteria and had a rods shape. Analysis of 16S rRNA gene sequence showed that isolate A13 had a similarity with Pseudochrobactrum assacharolyticum. Hypersensitivity assay on tobacco leaves showed that isolate A13 was not a pathogen. During 48 hours of incubation, isolate A13 produced a maximum of IAA at the 24th hour of incubation. Isolate A13 produced 0.675 ppm of ethylene/hour in Acetylene Reduction Assay and 69,839 ppm of IAA in HPLC methods. This was the first report on nitrogen fixation and IAA production by Pseudochrobactrum assacharolyticum and its application in the soil of oil palm seedlings. Application of isolate A13 in oil palm seedling increased significantly the number of lateral roots, stem diameter, and height of plants
Identification of Drought-tolerant Local Cowpea Varieties of Southwest Maluku (Indonesia) Karuwal, Ritha Lusian; Suharsono, Suharsono; Tjahjoleksono, Aris; Hanif, Novriyandi
Makara Journal of Science Vol. 22, No. 4
Publisher : UI Scholars Hub

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Abstract

Cowpeas grown in Southwest Maluku have local potential due to their diversity and ability to adapt to drought stress conditions that otherwise cause low productivity. The purpose of this study was to identify of local cowpea varieties of Southwest Maluku that show tolerance to drought. The experimental samples were seven local varieties and threereference cultivars. The drought stress treatment was altered watering periods, i.e., every two (P0) and ten days (P1) from growth to harvest. The results of this research showed that a ten-day watering period significantly decreased plant height, number of leaves, number of root nodules, root and shoot dry weight, photosynthesis rate, stomatal conductance, transpiration rate, relative water content, media water content, chlorophyll content, and number and weight of seeds perplant. Drought stress treatment increased proline content and root length. Correlation analysis showed a significant correlation between plant height with all variables, except for root length, proline content, and weight of seedsper plant. The correlation and cluster analyses showed that the KM7 variety is a drought tolerant genotype among the local cowpea varieties from Southwest Maluku. Therefore, the KM7 variety can be used as plant material in future breeding programs.
Transformation of Inhibitor of Meristem Activity (IMA) Gene into Jatropha curcas L. Paserang, Asri Pirade; Tjahjoleksono, Aris; Widyastuti, Utut; Suharsono,
Makara Journal of Science Vol. 19, No. 3
Publisher : UI Scholars Hub

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Abstract

Jatropha is one of the many biodiesel plants developed in tropical countries. Efforts to increase its productivity can be done using various methods of breeding. One of the breeding methods is the introduction of genes into the Jatropha plant. The aim of this study is to assess the success of genetic transformation using the Inhibitor of Meristem Activity (IMA) gene in Jatropha curcas. The research procedures included inoculation of explants with Agrobacterium tumefaciens, callus induction, screening test of selection media, regeneration, and gene expression analysis using Polymerase Chain Reaction (PCR). IMA is one of the genes that controls flowering genes and ovule development. It was first isolated from tomato plants and has been successfully overexpressed in these plants using the Cauliflower Mosaic Virus (CaMV) 35S promoter. In this experiment, plant transformation was performed on J. curcas as the target. Explant callus formation in both the control and treated samples was good, but shoot formation decreased dramatically in the treated explants. PCR analysis indicated that IMA genes can be inserted into J. curcas with the size of the IMA gene is 500 bp.
Establishment of transgenic potato cultivar IPB CP1 plants containing gene encoding for superoxide dismutase to increase the abiotic stress tolerance Musawira Musawira; Suharsono Suharsono; Miftahudin Miftahudin; Aris Tjahjoleksono
Indonesian Journal of Biotechnology Vol 27, No 3 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.68040

Abstract

Potato ( Solanum tuberosum L.) cultivar IPB CP1 is suitable as a raw material for the potato chip industry. Potato plants are sensitive to various abiotic stresses such as drought, aluminium and salinity, which induce reactive oxygen species (ROS). ROS is very toxic to plant cells. Superoxide dismutase (SOD) is one of the enzymes that catalyse ROS to H2O2 and O2. This study aimed to establish transgenic potato cv. IPB CP1 plants containing the MmCuZn‐SOD gene that are tolerant to various abiotic stresses. Genetic transformation using internodes without buds as explants produced putative transgenic potato with a transformation efficiency of 51.25% and a regeneration efficiency of 38.87%. Integration analysis of the MmCuZn‐SOD transgene in putative transgenic plants by polymerase chain reaction (PCR) with a set of specific primers showed that eight plants contained the MmCuZn‐SOD gene under the control of the 35S CaMV promoter. In vitro salinity stress, aluminium stress, and drought stress assays showed that transgenic plants had a higher number of roots and total root length than non‐transgenic ones. These results indicate that transgenic potato cv. IPB CP1 plants are more tolerant to abiotic stresses than non‐transgenic ones.
OPTIMIZATION OF Agrobacterium-MEDIATED GENETIC TRANSFORMATION OF oshox4 GENE IN SATOIMO TARO (Colocasia esculenta var. antiquorum) Febrina Ariyanti Ismail; Aris Tjahjoleksono; N Sri Hartati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.43 KB) | DOI: 10.29122/jbbi.v9i1.3063

Abstract

Satoimo (Colocasia esculenta var. antiquorum) can be considered as an alternative food to support food diversification. The aim of this research was to obtain an optimum condition of the genetic transformation method of Satoimo taro through Agrobacterium-mediated genetic transformation using binary vector harboring gene construct of oshox4 and hpt gene as a selectable marker to produce transgenic plants. Plant materials that were used in this study were meristem, root, leaf, petiole, and basal stem. Transformation procedures were carried out using three different co-cultivation periods (1, 2, and 3 days) and different optical densities of A. tumefaciens cells (0,5 and 0,7). Furthermore, callus induction was performed on MS medium containing 2.4-D, cefotaxime, hygromycin and then regenerated on MS medium containing thidiazuron and hygromycin. The putative transgenic plantlets were selected on MS medium containing hygromycin and analyzed using PCR. The results showed that putative transgenic plants derived from the basal stem could survive on a selection medium containing hygromycin and based on PCR analysis some of them contained the oshox4 and hpt genes. Satoimo (Colocasia esculenta var. antiquorum) dapat dijadikan sebagai alternatif pangan untuk mendukung penganekaragaman pangan. Tujuan penelitian ini adalah mendapatkan kondisi optimal untuk transformasi genetik talas Satoimo menggunakan gen oshox4 yang dimediasi oleh Agrobacterium tumefaciens. Transformasi genetik dilakukan dengan menggunakan vektor biner yang mengandung gen oshox4 dan gen hpt. Bahan tanaman yang digunakan dalam penelitian ini adalah meristem, akar, daun, tangkai daun dan pangkal batang talas Satoimo. Transformasi dilakukan dengan menggunakan tiga perlakuan waktu ko-kultivasi (1, 2, dan 3 hari) serta dua optical density (0,5 dan 0,7). Selanjutnya dilakukan induksi kalus pada media MS yang mengandung 2.4-D, cefotaksim, hogromisin dan diregenerasikan pada media MS yang mengandung thidiazuron dan higromisin. Plantlet putatif transgenik diseleksi menggunakan media MS yang mengandung higromisin serta dianalisis menggunakan PCR. Hasil penelitian menunjukkan bahwa tanaman putatif transgenik yang berasal dari pangkal batang dapat bertahan hidup pada media seleksi yang mengandung higromisin dan berdasarkan analisis PCR, beberapa di antaranya mengandung gen oshox4 dan gen hpt.
Peranan Pseudomonas aeruginosa ATCC 15442 terhadap Pertumbuhan Tanaman Cabai Rawit (Capsicum frutescens L.) Varietas Bonita pada Kondisi Salin: The Role of Pseudomonas aeruginosa ATCC 15442 on the Growth of Bonita Hot Pepper (Capsicum frutescens L.) Variety in Saline Conditions Aas Ratnasari; Nisa Rachmania Mubarik; Aris Tjahjoleksono
Jurnal Sumberdaya Hayati Vol. 9 No. 4 (2023): 2023
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.9.4.171-177

Abstract

Hot pepper (Capsicum frutescens L.) is one of the food crop commodities that is widely cultivated in Indonesia. Salinity stress can reduce the bioavailability of potassium and its uptake by plants, which will ultimately reduce plant growth and production. One way to reduce the effect of salinity and increase potassium uptake by plants is to use potassium-solubilizing bacteria. One of the bacteria that can solubilize potassium is Pseudomonas aeruginosa. This research aims to study the role of Pseudomonas aeruginosa ATCC 15442 on the growth of the Bonita variety of hot pepper under saline conditions. This study used a completely randomized factorial design with four concentration levels of NaCl treatment: 0, 4, 8, and 12 g/L, as well as two levels of bacteria administration: without bacteria and with bacteria. The results showed that the application of bacteria, salt concentration, and the interaction of the two had no effect (p-value> 0.05) on the growth of hot pepper plants with the observed parameters namely plant height, number of leaves, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, and chlorophyll content.
Analisis Metabolomik pada Interaksi Padi dan Bakteri: Metabolomics Analysis on the Interaction of Rice and Bacteria Theowidavitya , Brian; Muttaqin, Mafrikhul; Miftahudin; Tjahjoleksono, Aris
Jurnal Sumberdaya Hayati Vol. 5 No. 1 (2019)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.5.1.18-24

Abstract

Mutualistic plant-microbe interaction can increase the availability or absorption of nutrients and affect plant metabolism. Metabolomics is the study of all metabolites contained in living beings at a specific time. This research aims to study the effect of metanotrophic bacteria (BGM 9 isolate) and nitrogen-fixing bacteria (ITJ 7 isolate) on rice plant growth and reveal the information about metabolites produced by rice plant interacting with the bacteria. Three rice plant varieties (Ciherang, Hawara Bunar, and IR64) were grown on liquid media (Yoshida solution) and were inoculated with the bacteria five weeks after planting. Metabolites in the liquid media were detected seven days after inoculation using Liquid Chromatography-Mass Spectrometry and analyzed using MassLynx v4.1. The results showed that the bacterial inoculation had significant effect on rice growth. Chromatograms resulted from LC-MS showed there are 34 unique peaks from Ciherang, 21 unique peaks from Hawara Bunar, and 30 unique peaks from IR64. The unique peaks in each variety were affected by the bacteria inoculation treatments.
Stek Pucuk Kentang (Solanum tuberosum L.) Kultivar IPB CP1 Dapat Menghasilkan Umbi G0 di Dalam Rumah Kaca dan Rumah Kasa di Dataran Menengah: Shoot Cuttings of Potato Plant (Solanum tuberosum L.) Cultivar IPB CP1 Can Produce G0 Tubers in Greenhouses and Screen Houses in the Med-Altitude Area Fatimah, Anggi Siti; Miftahudin; Tjahjoleksono, Aris
Jurnal Sumberdaya Hayati Vol. 10 No. 2 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.2.78-84

Abstract

IPB CP1 potato is a raw material for potato chips. The advantage of IPB CP1 potatoes is their high yield. Challenges in potato production are limited areas with optimal altitude and condition as well as low quality and quantity of seeds. Utilizing a mid-altitude area for potato cultivation is one of the solutions to increase potato seed production. This study aimed to compare the growth and production of IPB CP1 G0 seed tuber in a greenhouse and screenhouse in mid-altitude with different numbers of shoot cuttings per polybag. This research was conducted with two factors. The first factor is the use of growth plant houses, i.e. greenhouse and screenhouse. The second factor is the number of shoot cuttings per polybag, i.e. 2 and 3 shoot cuttings per polybag. Growth parameters observed included plant height, number of nodes and leaves, and fresh and dry weight of shoots. The production parameters observed included the number as well as fresh and dry weight of tubers. The growth and production of IPB CP1 potatoes grown in the greenhouse were better than those grown in the screenhouse. The number of cuttings per polybag did not affect the growth and production of IPB CP1 potato.
Drought Tolerance in Transgenic Potato Cultivar IPB CP1 Expressing MmCuZn-SOD Gene Musawira; Tjahjoleksono, Aris; Hamim, Hamim; Miftahudin, M.
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1377-1386

Abstract

Drought stress induces reactive oxygen species (ROS) accumulation, which is harmful to plant cells. Consequently, it inhibits plant growth and decreases yield. The enzyme superoxide dismutase (SOD), which is encoded by the SOD gene, is the first defence enzyme in the cells that detoxify ROS. The study aimed to analyze the tolerance of transgenic potato cultivar IPB CP1 and its yield under drought-stress conditions. The results showed that transgenic plants had superior morphological characteristics, such as plant height, number of leaves, stem diameter, and plant biomass, than non-transgenic plants. However, photosynthetic rate, chlorophyll content, stomatal conductance, and transpiration rate showed similar levels between the transgenic and non-transgenic plants. The transgenic plants expressing the MmCu/Zn-SOD gene showed lower lipid peroxidation levels than the non-transgenic plants, indicating that the gene works well to reduce the cell's ROS level. Transgenic plant clone CP1S6 showed 13 times higher gene expression and tuber yield than non-transgenic plants. These research indicated that the transgenic plants expressing the MmCu/Zn-SOD gene are more tolerant to drought stress than the non-transgenic plants.
Co-Authors . SUHARSONO Aas Ratnasari Ai Nurhasanah Husnul Izzati Antonius Suwanto Aris Tri Wahyudi Asri Pirade Paserang Diah Ratnadewi Dwinita Wikan Utami Edi Husen Fatimah, Anggi Siti Febrina Ariyanti Ismail Fitri Damayanti Gustania, Leolita Hamim Hamim HIASINTA F. J. MOTULO Idha Susanti Idha Susanti Ika Mariska Ismi Isti'anah Karuwal, Ritha Lusian MEITY S. SINAGA Miftahudin Miftahudin . Miftahudin Miftahudin Miftahudin, M Miftahudin, M. Muhammad Faiz Amri Musawira Musawira Musawira MUTTAQIN, MAFRIKHUL N Sri Hartati NISA RACHMANIA MUBARIK Novriyandi Hanif Nurhasanah, Eka Ovi Prasetya Winandari Pasmawati Pasmawati Ritha Lusian Karuwal ROHANI CINTA BADIA GINTING SIENTJE MANDANG Suaidi, Malik Nuris Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono, Suharsono, Suharsono Theowidavitya , Brian Ulfah Juniarti Siregar Utut Widyastuti Utut Widyastuti Utut Widyastuti UTUT WIDYASTUTI Windarti Wahyuningtyas