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All Journal HAYATI Journal of Biosciences Jurnal Hortikultura Indonesia (JHI) AL KAUNIYAH Jurnal Penelitian Tanaman Industri Indonesian Journal of Biotechnology Jurnal Sumberdaya Hayati JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Journal of Microbial Systematics and Biotechnology Jurnal Penelitian Tanaman Industri (Littri) Berkala Penelitian Hayati Makara Journal of Science Annales Bogorienses Biodiversitas Journal of Biological Diversity
Aris Tjahjoleksono
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Institut Pertanian Bogor. Jl. Raya Dramaga, Kampus IPB Darmaga, Bogor 16680, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Stability of Morphophysiological Characters, Tuber Yield, and FBPase Gene Expression in Transgenic Potato Cultivar IPB-CP3 Suaidi, Malik Nuris; Tjahjoleksono, Aris; Miftahudin, M
Al-Kauniyah: Jurnal Biologi Vol. 19 No. 1 (2026): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v19i1.43558

Abstract

Potatoes are an essential agricultural commodity that needs to be genetically improved to increase productivity and meet industrial needs. The research aimed to evaluate the stability of the morpho-physiological characters, tuber yield, and transgene expression of transgenic potato cultivar IPB-CP3 harboring the FBPase gene in the G0 and G1 generations. The study was conducted by growing transgenic plants in a greenhouse and analyzing plant morpho-physiological characters, tuber yield, and gene expression. The results showed that transgenic plants had higher values in stem diameter (32.7–50.8%), number of leaves (33.9–41.2%), biomass dry weight (14.4–36.2%), photosynthetic rate (25.2–28.25%), and tuber weight (42.6–196.0%) than non-transgenic plants. However, there was no significant difference in plant height characters between the transgenic and non-transgenic plants. Transgenic plants consistently expressed the FBPase gene at higher level than the non-transgenic plants in the G0 and G1 generations. The research results suggest that overexpression of the FBPase gene increases plant growth and tuber yield. This finding implies agricultural practices, particularly in the context of crop improvement through genetic engineering.
Manipulasi Nutrien dan Zat Pengatur Tumbuh untuk Meningkatkan Produksi dan Kualitas Buah Stroberi (Fragaria x ananassa) Kultivar BAT-1 Tjahjoleksono, Aris; Gustania, Leolita; Ratnadewi, Diah
Jurnal Hortikultura Indonesia (JHI) Vol. 14 No. 2 (2023): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.14.2.63-70

Abstract

Stroberi kultivar BAT-1 semakin jarang dibudidayakan oleh petani Indonesia karena rasa buahnya kurang manis, padahal kultivar tersebut mampu beradaptasi dengan kondisi lingkungan tropis. Hal tersebut disebabkan perbaikan kualitas dan produktivitas stroberi adaptif itu masih sulit dilakukan dengan praktik pertanian biasa. Tujuan penelitian ini adalah untuk meningkatkan produksi dan kualitas buah stroberi BAT-1, yaitu ukuran, warna, rasa manis dan umur simpan, dengan memodifikasi nutrien dan manipulasi zat pengatur tumbuh (ZPT). Penelitian ini dilaksanakan pada Agustus – Desember 2021 (musim hujan) dan Februari – Juni 2022 (musim kemarau), di kaki Gunung Halimun-Salak, Kabupaten Bogor. Penelitian menggunakan rancangan acak lengkap faktorial: Perlakuan tahap pertama berupa pemberian kalium dan kalsium, dan diukur parameter vegetatifnya. Untuk tahap kedua diberikan perlakuan asam indol butarat dan brassinolida, dan diukur parameter generatifnya. Hasilnya, perlakuan kalium dan kalsium mempercepat dan mempersingkat rentang waktu berproduksi tanaman; dan modifikasi nutrien yang berkombinasi dengan ZPT mampu meningkatkan bobot buah, kandungan antosianin buah, nilai kemanisan serta menurunkan nilai total asam. D3I3B2 (PPC + CaCl2 2 g L-1 pada daun dan ZPT IBA 3 mM + BL 0.2 µM pada buah) merupakan perlakuan terbaik, di kedua musim tanam. Brassinolida (0.2 µM) mampu memperlama daya simpan buah setelah panen.-1Kata kunci: Brassinolide, brix, IBA, kalium, kalsium.
Optimization of Somatic Embryogenesis Induction of Cassava (Manihot esculenta Crantz) Susanti, Idha; Suharsono, Suharsono; Widyastuti, Utut; Siregar, Ulfah Juniarti; Tjahjoleksono, Aris
Annales Bogorienses Vol. 21 No. 2 (2017): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The embryogenesis (SE) has important role for genetic engineering of cassava (Manihot esculenta Crantz). However, the success of SE induction depend on plant growth regulator s (PGR)s and treatment enriched in induction media. This experiment tried to induce callus formation of cassava from several in vitro explants: immature leaf, apical bud, and internode; and to develop somatic embryogenesis of cassava in several media enriched with tyrosine and copper sulphate (CuSO4) added into media enrich with picloram as treatment. Different response of explants source to callus induction treatment from those three varieties in callus induction as well as friable callus formation were found in this experiment. The best medium to induce varied with variety; MS media supplemented 12 mg/L picloram + 0.5 mg/L CuSO4 was the best for “Adira 4” and half MS and half GD media supplemented 12 mg/L picloram + 100 mg/L tyrosine for “Malang 6”. All treatments resulted somatic embryo which developed indirectly and in morphologically normal somatic embryos
Genetic Engineering of Potato (Solanum tuberosum L.) IPB CP2 Variety with OsGERLP Gene: Genetic Transformation of Potato (Solanum tuberosum L.) IPB CP2 Variety with OsGERLP Gene Nurhasanah, Eka; Tjahjoleksono, Aris; Miftahudin, M.
Jurnal Sumberdaya Hayati Vol. 11 No. 4 (2025): 2025
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.11.4.137-145

Abstract

High dissolved aluminum (Al) content in acidic soil can inhibit root elongation, especially in plants sensitive to Al. The OsGERLP gene is known to be an Al tolerance gene in rice, but the role of this gene in other plants is not yet known. Introduction of the OsGERLP gene to potato cv. IPB CP2 mediated by Agrobacterium tumefaciens has been succesfully conducted. When using leaf explant, the transformation and regeneration efficiencies were 72.62 and 17.24%, respectively. However, transgene introduction using internode explant increased transformation and regeneration efficiencies to 71.3 and 87.65%, respectively. PCR-based transgene integration analysis showed that all transgenic clones contain the OsGERLP gene under the control of the 35ScaMV promoter, which was indicated by the formation of 1500 bp amplicons. Transgenic clones have been in vitro tested for Al stress using liquid MS medium with different pH and Al content. The result showed that all transgenic clones have higher tolerance to Al compared to non-transgenic plant. Morphological analysis showed that transgenic clones performed better in all parameters including plant height, total root length, number of roots, shoot and root fresh weight, shoot and root dry weight. The transgenic clones can potentially be developed as potato transgenic lines tolerant to Al toxicity.
Introduction of the OsGERLP Gene into Potato cv. IPB CP3 to Develop Aluminum Stress-Tolerant Potato Lines Mahfudhah, Farah; Tjahjoleksono, Aris; Miftahudin, M.
HAYATI Journal of Biosciences Vol. 33 No. 3 (2026): May 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.3.646-656

Abstract

The OsGERLP gene is an aluminum (Al) stress tolerance gene. Potato cv. IPB CP3 is a horticultural crop that has not been proven to be tolerant to Al; therefore, enhancing its tolerance through genetic transformation is necessary. This research aims to obtain transgenic potatoes cv. IPB CP3 contains the OsGERLP gene and is tolerant to Al stress. Experimental methods include transforming potatoes with the OsGERLP gene via Agrobacterium tumefaciens, transgene integration testing, in vitro assays of transgenic plants under low pH and Al stress, and analysis of transgene expression. The results showed that the transformation efficiency achieved was relatively high at 47.03%, with a regeneration efficiency of 42.19%. The transgenic clones had longer roots and more roots than the non-transgenic ones under aluminum stress. The transgenic clones GERLP2, GERLP3, and GERLP4 exhibited the greatest root growth enhancement under stress conditions and the highest OsGERLP gene expression levels. These clones have the potential to be developed into Al-tolerant potato varieties. Future research is required to evaluate aluminum stress tolerance, tuber yield performance, and transgene stability across the three clones under greenhouse and field conditions of the three clones.
Physiological responses of some local cowpea from Southwest Maluku (Indonesia) varieties to drought stress RITHA LUSIAN KARUWAL; SUHARSONO SUHARSONO; ARIS TJAHJOLEKSONO; NOVRIYANDI HANIF
Biodiversitas Journal of Biological Diversity Vol. 18 No. 4 (2017)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d180402

Abstract

Karuwal Rl, Suharsono, Tjahjoleksono A, Hanif N. 2017. Physiological responses of some local cowpea from Southwest Maluku (Indonesia) varieties to drought stress. Biodiversitas 18: 1294-1299. The aim of this study was to analyze the physiological responses of some local cowpea from Southwest Maluku (Indonesia) varieties to drought stress. The physiological responses were analyzed by measuring plant height, number of leaves, relative water content (RWC), and chlorophyll content using Anova and were continued with Duncan test at 5% significance level. Results of this research showed that the varieties and drought stress in the form of watering periods to affect to all variables except plant height. Physiological responses showed that at every ten days watering periods, the crimson varieties gives the highest plant height (33.85 cm), the brown varieties gives the highest number of leaves (30), the dark brown varieties have the highest RWC (88.675%), and the highest content of chlorophyll a (0.5088 mg/L), chlorophyll b (1.595 mg/L), and total chlorophyll (1.5095 mg/L) are found in white varieties. The supply of water at every ten days is the optimum time of drought stress in cowpea. Further research will be needed on the responses of the other variables for screening, selection, then multi-location trials to obtain tolerant local cowpea varieties to drought.
Correlation among Snpb11 markers, root growth, and physiological characters of upland rice under aluminum stress MIFTAHUL HUDA FENDIYANTO; RIZKY DWI SATRIO; SUHARSONO SUHARSONO; ARIS TJAHJOLEKSONO; M MIFTAHUDIN
Biodiversitas Journal of Biological Diversity Vol. 20 No. 5 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200514

Abstract

Abstract. Fendiyanto MH, Satrio RD, Suharsono, Tjahjoleksono A, Miftahudin. 2019. Correlation among Snpb11 markers, root growth, and physiological characters of upland rice under aluminum stress. Biodiversitas 20: 1243-1254. The cultivation of upland rice in acid soils faces aluminum (Al) toxicity. Development of Al-tolerant rice could be one of the solutions to overcome the problem. Marker-assisted breeding to develop Al-tolerant rice requires at least a molecular marker for foreground selection. Snpb11 is a molecular marker developed from the nucleotide differences in a specific allele between Al-tolerant and sensitive rice. Snpb11 has never been used as a molecular marker in rice. Therefore this study aimed to examine the correlation among Snpb11 marker, root growth, and physiological characters under Al stress in upland rice. We used physiological characters and the Snpb11 marker to justify the Al tolerance level in several upland rice varieties. We found that physiological characters, i.e.: primary root length, total root length, chlorophyll, and carotenoid content showed positive correlation to Snpb11. Conversely, root malondialdehyde content, which represents the level of lipid peroxidation showed a negative correlation to Snpb11. There is evidence that the Snpb11 highly correlated with primary and total root length characters, which are the Al tolerance parameters used in rice. Therefore, Snpb11 markers can be used to distinguish the Al tolerance level in upland rice.
Endophytic actinobacteria of Eleutherine palmifolia as antioxidant producer AFINA PUTRI SHABIRA; ARIS TJAHJOLEKSONO; YULIN LESTARI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 8 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230844

Abstract

Abstract. Shabira AP, Tjahjoleksono A, Lestari Y. 2022. Endophytic actinobacteria of Eleutherine palmifolia as antioxidant producer. Biodiversitas 23: 4209-4215. Endophytic actinobacteria can generate bioactive compounds similar to those produced by the host plant. The Eleutherine palmifolia (L.) Merr is an example of a widely utilized host plant in the Dayak community. Flavonoids, which have antioxidant effects, are among the bioactive compounds found in E. palmifolia. The antioxidant activity of bioactive compounds produced by endophytic actinobacteria of E. palmifolia remains unidentified. The aim of this study was to examine the antioxidant activity of bioactive compounds produced by endophytic actinobacteria of E. palmifolia. Antioxidant activity from isolates actinobacteria was determined quantitatively using the DPPH and ABTS methods. Identification of the types of bioactive compound in the samples was identified using TLC assay and bioautography test. Analysis of flavonoid levels was performed using colorimetric aluminum chloride (AlCl3). The result showed that the extraction of six isolates of endophytic actinobacteria with ethyl acetate solvent had different yields. The extract of endophytic actinobacteria EPB 6 isolate produced the highest (0.013%) yield. The extract of endophytic actinobacteria EPB 3 isolate had the best antioxidant activity, with 53.85 mg/mL (DPPH) and 42.43 mg/mL (ABTS). The extract of endophytic actinobacteria EPB 3 isolate and E. palmifolia had the same blue spots in TLC analysis, indicating the presence of bioactive compounds flavonol and flavones groups. The bioautography test of EPB 3 isolate endophytic actinobacteria extract produced yellow color, indicating qualitatively antioxidant activity. The total flavonoid content of the EPB 3 isolate extract was also higher (25.30 QE/mg) than the other five isolates, while it was 46.03 QE/mg in E. palmifolia extract. It is clear from the present study that the endophytic actinobacteria extract can produce antioxidants.
Peat-derived Streptomyces spp. isolated from edamame rhizosphere with plant growth-promoting properties IRA ERDIANDINI; ARIS TJAHJOLEKSONO; RIKA INDRI ASTUTI; EDI HUSEN; ARIS TRI WAHYUDI
Biodiversitas Journal of Biological Diversity Vol. 26 No. 1 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260132

Abstract

Abstract. Erdiandini I, Tjahjoleksono A, Astuti RI, Husen E, Wahyudi AT. 2025. Peat-derived Streptomyces spp. isolated from edamame rhizosphere with plant growth-promoting properties. Biodiversitas 26: 326-334. Peatlands, which are known for their high organic matter content, are common habitats for actinomycetes. These microorganisms have been recognized for their potential as plant growth promoters. However, there have been limited reports on peat-derived actinomycetes with plant growth-promoting properties, especially in edamame-cultivated peatlands. This study aimed to isolate and investigate plant growth-promoting actinomycetes in the rhizosphere of edamame-cultivated peatlands. The results showed that a total of 46 strains were isolated from the edamame rhizosphere during the anthesis and reproductive phase. Importantly, 36 of these strains were found to be biologically safe, as showed by the negative hemolysis and hypersensitivity test. All 36 strains produced indole-3-acetic acid in the range of 2.42 to 50.07 µg/mL. Based on the in vivo plant growth-promoting activity assay, strains RT34, AR26, AR39, and BT59 promoted the highest primary root growth of edamame sprouts. Interestingly, the acetylene reduction assay revealed that only RT34 and AR39 strains exhibited nitrogenase activity as high as that of Azotobacter sp. as a positive control. The nitrogenase activity of these strains was up to 27.42 nmol C2H4 h-1tube-1 and 28.10 nmol C2H4 h-1tube-1. Furthermore, quantitative phosphate solubilization assay showed phosphate solubilization up to 527.76 µg/mL in the range of 258.31 to 527.76 µg/mL. Based on 16S rRNA sequencing, these strains were closely related to the genus Streptomyces. The finding of present results strongly indicates that these Streptomyces strains have plant growth-promoting properties and can be proposed as biostimulants to enhance edamame growth in peatlands.
Co-Authors . SUHARSONO Aas Ratnasari AFINA PUTRI SHABIRA Ai Nurhasanah Husnul Izzati Antonius Suwanto Aris Tri Wahyudi Asri Pirade Paserang Diah Ratnadewi Dwinita Wikan Utami EDI HUSEN Edi Husen Fatimah, Anggi Siti Febrina Ariyanti Ismail Fitri Damayanti Gustania, Leolita Hamim Hamim HIASINTA F. J. MOTULO Idha Susanti Idha Susanti Ika Mariska IRA ERDIANDINI Ismi Isti'anah Karuwal, Ritha Lusian M MIFTAHUDIN Mahfudhah, Farah MEITY S. SINAGA Miftahudin Miftahudin . Miftahudin Miftahudin Miftahudin, M Miftahudin, M. MIFTAHUL HUDA FENDIYANTO Muhammad Faiz Amri Musawira MUTTAQIN, MAFRIKHUL N Sri Hartati NISA RACHMANIA MUBARIK Novriyandi Hanif Nurhasanah, Eka Ovi Prasetya Winandari Pasmawati Pasmawati Rika Indri Astuti RITHA LUSIAN KARUWAL Ritha Lusian Karuwal RIZKY DWI SATRIO ROHANI CINTA BADIA GINTING SIENTJE MANDANG Suaidi, Malik Nuris Suharsono Suharsono Suharsono Suharsono SUHARSONO SUHARSONO Suharsono Suharsono SUHARSONO SUHARSONO Suharsono Suharsono Suharsono Suharsono Suharsono, Suharsono, Suharsono Theowidavitya , Brian Ulfah Juniarti Siregar Utut Widyastuti UTUT WIDYASTUTI Utut Widyastuti Utut Widyastuti Windarti Wahyuningtyas YULIN LESTARI