Alimsardjono, Lindawati
Departemen Mikrobiologi Kedokteran, Fakultas Kedokteran, Universitas Airlangga

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Progress In Knowledge Of Microscopic Tuberculosis Diagnosis Laboratory Training Participants Silvia Sutandhio; Ni Made Mertaniasih; Eko Budi Koendhori; Deby Kusumaningrum; Titiek Sulistyowati; Lindawati Alimsardjono; Pepy Dwi Endraswari; Manik Retno Wahyunitisari; Rebekah Juniati Setiabudi
JURNAL WIDYA MEDIKA Vol. 5 No. 2 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v5i2.2202

Abstract

Indonesia is the second highest tuberculosis (TB)-burden country in the world. TB affects men and women in productive ages. Early and accurate diagnosis of TB is crucial in TB management because it allows prompt treatment with appropriate regimen. Sputum smear microscopy is the most common method used in resource-limited laboratories. The aim of this study is to assess whether adult learning method with student-centered approach can improve knowledge in laboratory techniques in microscopic TB diagnosis. It was a quasi experimental research with one group pretest and posttest design. Pretest was conducted before the training started. During the training, participants attended lectures, discussion sessions, and hands-on laboratory activities. After training, participants were asked to respond to posttest. There were 33 participants of this training (n=33). Paired t-test analysis showed significant difference between pretest and posttest scores (p=6.5x10-9). Variance between pretest scores was 4.00 and variance between posttest scores were 1.34. Pearson correlation coefficient was 0.45. Regardless their level of knowledge before training, participants could achieve relatively equivalent results after training. Significant difference between pre- and post-training test scores indicated that the learning method was effective and the training could improve participants knowledge in laboratory techniques in microscopic TB diagnosis.
The Correlation between icaA and icaD Genes with Biofilm Formation Staphylococcus epidermidis In Vitro Rachmawati, Dian; Kuntaman, Kuntaman; Alimsardjono, Lindawati
Folia Medica Indonesiana Vol. 55, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

This study was conducted to identify the presence of icaA and icaD genes in S. epidermidis and to analyze the relationship between the presence of icaA and icaD genes with the ability of in vitro biofilm formation in S. epidermidis. S. epidermidis isolates from patients and healthy people were collected and PCR was examined to detect icaA and icaD genes. which then continued to examine the ability of biofilm formation by the method of Congo Red Agar. The results of this genotypic and phenotypic examination were then tested for correlation with statistical tests using SPSS 23.0. A total of 40 S. epidermidis isolates were collected, consisting of 20 clinical isolates and 20 isolates of normal flora. The icaA gene was positive in 5 isolates (12.5%), and 8 isolates (20%) were positive for the icaD gene, 3 isolates with icaA and icaD were both positive. One hundred percent of isolates with icaA or icaD positively formed biofilms, but there were 15 isolates (42.9%) who did not have the icaA gene but showed the ability to form biofilms, while 12 isolates (37.5%) who did not have the icaD gene also formed biofilms. Fifty percent of S. epidermidis isolates showed the ability to form biofilms at CRA. The Fisher Exact test showed a significant relationship between the icaA gene and the ability of biofilm formation (p=0.047 (p<0.05)) as well as the icaD gene (p=0.03 (p<0.05)). The icaA and icaD genes have a significant relationship to biofilm formation in S. epidermidis. There was another mechanism in the formation of biofilms that are not dependent on the ica gene.
Edukasi Resistensi Antimikroba pada Siswa SMA: Upaya Membangun Kesadaran Penggunaan Antibiotik yang Bijak: Upaya Membangun Kesadaran Penggunaan Antibiotik yang Bijak Koendhori, Eko Budi; Dwi Endraswari, Pepy; Kusumaningrum, Deby; Mahmudatul Muna, Nafdzu; Juniastuti, Juniastuti; Wiqoyah, Nurul; Retno Wahyunitisari, Manik; J. Setiabudi, Rebekah; Wiwin Retnowati; Setiawan, Firman; Alimsardjono, Lindawati
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 10 No 3 (2025): J-Dinamika
Publisher : Politeknik Negeri Jember

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Abstract

Latar Belakang: Resistensi antimikroba merupakan salah satu ancaman kesehatan global yang dapat meningkatkan angka kesakitan, kematian, dan biaya pelayanan kesehatan. Edukasi tentang penggunaan antibiotik yang bijak perlu diberikan sejak usia sekolah agar siswa dapat menjadi agen perubahan di masyarakat.Tujuan: Meningkatkan pengetahuan siswa SMA mengenai antibiotik dan resistensi antimikroba melalui edukasi interaktif.Metode: Kegiatan dilaksanakan pada 97 siswa SMA Al-Uswah Surabaya dengan metode seminar, diskusi, dan lomba media edukasi (video/poster). Evaluasi dilakukan melalui kuesioner pre-test dan post-test dengan 10 pertanyaan terkait antibiotik dan resistensi antimikroba. Analisis dilakukan dengan menghitung persentase jawaban benar dan peningkatan pengetahuan.Hasil: Rata-rata skor post-test meningkat dibandingkan pre-test. Peningkatan tertinggi pada pertanyaan mekanisme resistensi antibiotik (34,5%), terendah pada pemahaman dasar antibiotik (3,9%). Normalized gain (g=0,44) menunjukkan kategori peningkatan sedang.Kesimpulan: Edukasi interaktif efektif meningkatkan pemahaman siswa tentang resistensi antimikroba. Kegiatan serupa perlu dilanjutkan secara berkelanjutan dan diperluas ke sekolah lain.