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Antibacterial Effect Of Curcuma Domestica Ethanolic Extract On Group A Streptococcus Î’ Hemoyticus Halim, Stefan W; Wihanto, Laura; Sutandhio, Silvia
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 1, No 3 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Introduction : Pharyngitis is one of the most common infection that general practitioners handled. It is estimated in one year there are 15 million people with pharyngitis will come to the doctor. American Society of Microbiology said that 94,3 % from 402 patient who were suspected pharyngitis received antibiotic therapy even without indication of antibiotic therapy. Antibiotic resistance is becoming a great threat for the world today. World Health Organization state that one of the plan to fight antibiotic resistance is to research and develop new drugs. Curcuma domestica is a plant that is widely used in Indonesia and some research said that it has anti-inflammatory, antioxidant, and antimicrobial effect. Aim: The aim of this study is to find the antibacterial effect of Curcuma domestica ethanolic extract on Group A Streptococcus ? hemolyticus and find the Minimum Bactericidal Concentration (MBC) value. Methods : This was in vitro experimental study with broth microdilution method and inoculation on agar blood media to find the MBC. Treatment group consisting of, media (Mueller Hinton Blood Broth), Group A Streptococcus ? hemolyticus bacteria, and 5 concentrations (312.5 ?g/ml, 625 ?g/ml, 1250 ?g/ml, 2500 ?g/ml, 5000 ?g/ml) of Curcuma domestica extract. Result : There is no bacterial growth at 5000 ?g/ml concentration on solid media, but there are less bacterial growth at 2500 ?g/ml than at concentration 312.5 ?g/ml, 625 ?g/ml, 1250 ?g/ml. Conclusion: There is an antibacterial effect of Curcuma domestica ethanolic extract on Group A Streptococcus ? hemolyticus with MBC value in range 2500 ? 5000 ?g/ml.

The Comparison Of Antibacterial Effects On Cinnamomum Burmannii Water Extract With Penicillin Against Staphylococcus Aureus In Vitro Halim, Felicia; Dewi, Bernadette Dian Novita; Sutandhio, Silvia
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 2, No 1 (2020): January
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Introduction: Along with the development of science, many varieties of microorganisms are resistant to antibiotics. To avoid cases of resistance grew severe, researchers developed alternative medicines as a substitute for antibiotics. One of the ingredients of herbs researched that has antibacterial activity is Cinnamon (Cinnamomum burmannii). Aim: This study aimed to compare antibacterial effects water extract of Cinnamomum burmannii with penicillin against Staphylococcus aureus Methods: This an experimental studies witd non-equivalent control group design. Research using the bacteria S. aureus are divided into two groups, the control group and experimental group. The concentration of Cinnamomum burmannii and penicillin are used that is 80- 150mg/L. Antibacterial test used dilution method and a microplate reader for reading absorbance. This research conducted in the laboratory of Microbiology, Faculty of Pharmacy Widya Mandala Catholic University Surabaya for 2 weeks. Result: Based on visual observations, Cinnamomum burmanniiâ€™s MIC is located at concentration 320- 600 mg/L while penicillinâ€™s MIC is located at concentration 160- 300 mg/L. Based on the results of microdilution test, Cinnamomum burmanniiâ€™s MIC is located at concentration 160- 300 mg/L while penicillinâ€™s MIC is located at concentration 640- 1200 mg/L. Conclusion: The conclusion of this research is giving Cinnamomum burmannii water extract in Staphylococcus aureus has efficacy or inhibitory that is not significantly different with penicillin.

Comparation of Phenotypic and Genotypic Profile of Carbapenemase Producing Escherichia coli Silvia Sutandhio; Budiono Budiono; Hardiono Hardiono; Kuntaman Kuntaman; Eddy Bagus Wasito; Maria Inge Lusida
Folia Medica Indonesiana Vol. 54 No. 1 (2018): March
Publisher : Faculty of Medicine, Universitas Airlangga

Carbapenemase-producing Escherichia coli (E. coli) has caused trouble in therapeutic antibiotic selection. Carbapenemase screening procedure in laboratories is usually based on inacurate semi-automatic system. Confirmation and classification of carbapenemases according to Ambler can be done with combination of phenotypic methods, i.e., Modified Hodge Test (MHT), Sodium Mercaptoacetic Acid (SMA), and 3-Aminophenylboronic Acid (PBA). This study aimed to compare profiles of carbapenemase-producing E. coli which were confirmed and classified phenotypically with the genotypic profiles. E. coli isolates from urine specimens which were potential as carbapenemase-producers according to semi-automatic system BD Phoenix were phenotypically tested with MHT, SMA, and PBA. Isolates were grouped as carbapenemase-producers and non carbapenemase-producers. Phenotypic carbapenemase-producer isolates were classified based on Ambler criteria. All isolates were then tested with Polymerase Chain Reaction (PCR) for the presence of OXA-48, IMP1, IMP2, GES, VIM, NDM, KPC genes. Out of 30 isolates, 6 isolates (20.0%) were MHT positive, and 25 isolates (83.3%) were SMA positive, which indicated that most isolates produced were carbapenemase Ambler B. PCR confirmed 12 isolates (40.0%) had VIM gene which were classified as carbapenemase Ambler B. Phenotypic confirmatory test had 100% sensitivity and 22.2% specificity. Classification with phenotypic confirmatory test had 91.7% match with PCR. Phenotypic confirmatory test detected more carbapenemase than PCR. This low specificity may be caused by inappropriate use of diagnostic gold standard. PCR should not be used for routine carbapenemase confirmation because of vast diversity of carbapenemases. Phenotypic confirmatory test can classify carbapenemase according to Ambler classification.

The Effect of Cinnamomum burmannii Water Extraction Against Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans: In Vitro Study Novita, Bernadette Dian; Sutandhio, Silvia
Folia Medica Indonesiana Vol. 55 No. 4 (2019): December
Publisher : Universitas Airlangga

Indonesian cinnamon (Cinnamomum burmannii) is a native plant of Indonesia that has a lot of potential. The most consumed part is the bark. This study aims to examine the antimicrobial effect of Cinnamomum burmannii bark extract on various types of pathogenic microbes, namely Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans. An experimental study using a water extract (infusion) of Cinnamomum burmannii bark and a microbial test obtained from the Faculty of Pharmacy, Widya Mandala Catholic University, Surabaya. The antimicrobial effect test was carried out by the microdilution method in 96-well-microplate to determine the Minimum Inhibitory Level (MIC) and implantation on solid media to determine the Minimum Kill Rate (KBM). The MIC and KBM against Staphylococcus aureus were 625-1,250 ppm and 1,250-2,500 ppm, respectively. MIC and KBM for Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans were not found at the highest concentrations tested at 10,000 ppm. Cinnamomum burmannii extract can be used as a potential ingredient with antimicrobial effects, especially against Gram-positive bacteria. Future studies should pay attention to the quality of simplicia, particle size, and the most effective extraction methods extracting antimicrobial substances from simplicia

Distribusi Dan Pola Kepekaan enterobacteriaceae Dari Spesimen Urin Di RSUD DR. Soetomo Surabaya Periode Januari – Juni 2015 Silvia Sutandhio; Lindawati Alimsardjono; Maria Inge Lusida
JURNAL WIDYA MEDIKA Vol. 3 No. 1 (2015)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v3i1.775

Latar Belakang: Bakteri penyebab infeksi saluran kemih (ISK) didominasi oleh Enterobacteriaceae. Idealnya, setiap rumah sakit memiliki peta kuman dan pola kepekaan sendiri untuk digunakan sebagai panduan terapi empirik dan monitor penyebaran bakteri multiresisten. Hasil kultur urin, yang merupakan pemeriksaan penunjang untuk diagnosis dan pemilihan terapi antimikroba definitif, dapat dimanfaatkan untuk tujuan tersebut. Metode: Spesimen urin dikultur pada media isolasi primer, lalu diidentifikasi secara manual dan sistem semi-otomatis, yaitu BD Phoenix dan Vitek 2, yang telah dikonfirmasi dengan Clinical and Laboratory Standards Institute 2015. Hasil: Sebanyak 57.2% dari 1983 isolat hasil kultur teridentifikasi sebagai Enterobacteriaceae, dengan spesifikasi: 59.6%, 18.1%, 0.1%, 10.0%, dan 3.8%, berturut-turut untuk Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter spp., dan Proteus spp. Lebih dari 50% isolat Escherichia coli, Klebsiella pneumoniae, dan Klebsiella oxytoca merupakan penghasil Extended-Spectrum Beta Lactamase (ESBL). Kesimpulan: Enterobacteriaceae yang diisolasi umumnya resisten terhadap Ampisilin dan Sefalosporin generasi I, tetapi masih sensitif terhadap antimikroba golongan Karbapenem dan Aminoglikosida. Antimikroba golongan Karbapenem, yang merupakan pilihan terakhir pada kasus infeksi oleh Enterobacteriaceae multiresisten, hanya boleh diresepkan bila sesuai dengan indikasi, untuk mencegah timbulnya organisme resisten Karbapenem.

Perbandingan Distribusi Dan Pola Kepekaan Acinetobacter baumannii Terhadap Antimikroba Di RSUD Dr. Soetomo Surabaya Periode Januari–Maret 2015, April–Juni 2015, dan Januari–Maret 2016 Silvia Sutandhio; Agung Dwi Wahyu Widodo; Lindawati Alimsardjono; Eddy Bagus Wasito
JURNAL WIDYA MEDIKA Vol. 4 No. 1 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v4i1.1782

Acinetobacter baumannii is an environmental bacteria that can cause opportunistic infections such as ventilator-associated pneumonia, burn wound infection, urinary tract infection, and sepsis in immunocompromised patients. A. baumannii infections are troublesome because the bacteria resistance to many antimicrobial agents. Surveillance of distribution and sensitivity pattern of A. baumannii is best to be done frequently in hospitals.Venous blood as much as 10 milliliters was drawn aseptically and transferred into liquid medium Bactec® to be sent to Clinical Microbiology Laboratory of Dr. Soetomo General and Teaching Hospital. Medium that showed bacterial growth was subcultured on Blood Agar Plate and MacConkey Agar, and incubated for 18-24 hours in aerobic condition. Identification and susceptibility test were done with semi-automated system BD Phoenix, and interpreted based onClinical and Laboratory Standards Institute 2015.A. baumannii collected on trimester January-March 2015 were 30 isolates, with 19 isolates being Multidrug Resistant(MDR) A. baumannii (63%) and 6 isolatesPandrug Resistant(PDR)A. baumannii (20%). Isolates from trimester April-June 2015 were 32 isolates, with 21 isolates being MDR A. baumannii (66%) and 2 isolates PDR A. baumannii (6%). Isolates from trimester January-March 2016 were 40 isolates, with 22 isolates being MDR A. baumannii (55%) and 2 isolates PDR A. baumannii (5%).A. baumannii mostly isolated from Intensive Care Unit, followed by Internal medicine ward, Pediatric ward, Surgery ward and Emergency Department. High sensitivity of A. baumanniion trimester January-March 2016 was to imipenem (50%), meropenem (50%), amikacin (53%), cotrimoxazole (53%), dan cefoperazone-sulbactam (55%).. MDRA. baumannii mostly isolated from ICU, which reflects high burden of antimicrobial use. At this moment, antimicrobial agents that can be used as empirical therapy for A. baumannii infection are imipenem, meropenem, amikacin, cotrimoxazole, and cefoperazone-sulbactam. The entire hospital member must implement the Infection Control and Prevention and Antimicrobial Stewardship Program to preventnosocomial infections byA. baumanniiand the emergence of MDRA. baumannii.

Antimikroba: Magic Bullet Versus Superbugs Silvia Sutandhio; Lindawati Alimsardjono; Eddy Bagus Wasito
JURNAL WIDYA MEDIKA Vol. 4 No. 1 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v4i1.1807

Magic bullet is a terminology used by Paul Ehrlich to refer to substance that selectively attacks microbes that cause infection without affecting human tissues. The concept of magic bullet is adopted in attempts to find antimicrobial drugs that is safe to human. Improper and excessive use of antimicrobial drugs has caused the emergence ofmicrobes that are resistant to various antimicrobial drugs (superbugs). Superbugs are recent healthcare and public health issues. This condition is exacerbated bythe discovery void of effective antimicrobial drug against superbugs. Health practitioners must perform prudent use of antibiotics in order to prevent transmission of and infection by superbugs.

Distribusi dan Antibiogram MRSA dari Spesimen Darah selama Empat Semester di RSUD Dr. Soetomo Surabaya Silvia Sutandhio; Agung Dwi Wahyu Widodo; Eddy Bagus Wasito
JURNAL WIDYA MEDIKA Vol. 4 No. 2 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v4i2.1812

MRSA bloodstream infection is hospital-acquired problem that is difficult to treat. Bacteria spread to body organs, create multiple loci of infection, and survive in the presence of beta-lactam antibiotics. Study of distribution and antibiogram of MRSA from blood specimens was done in four semesters; i.e. July–December 2014, January–June 2015, July–December 2015, and January–June 2016. Blood of patients suspected for bloodstream infection was drawn aseptically, transferred into liquid medium, and sent to Clinical Microbiology Laboratory of Dr. Soetomo General Hospital. Medium with microbial growth was subcultured on solid medium, and incubated for 18-24 hours in aerobic condition. Identification and susceptibility test were done with BD Phoenix, and interpreted based on Clinical and Laboratory Standards Institute 2015. Of total 107 MRSA isolates; 23 isolates (22%) were collected on July–December 2014, 12 isolates (11%) on January–June 2015, 25 isolates (23%) on July–December 2015, and 47 isolates (44%) on January–June 2016. MRSA mostly isolated from internal disease wards (45%). Isolates are sensitive to linezolid (91%), quinupristin-dalfopristin (83%), fosfomycin (82%), and vancomycin (80%). Trend of MRSA bloodstream infection is increasing. Although antibacterial agents against MRSA are available, it is best to prevent MRSA transmissions and infections.

Progress In Knowledge Of Microscopic Tuberculosis Diagnosis Laboratory Training Participants Silvia Sutandhio; Ni Made Mertaniasih; Eko Budi Koendhori; Deby Kusumaningrum; Titiek Sulistyowati; Lindawati Alimsardjono; Pepy Dwi Endraswari; Manik Retno Wahyunitisari; Rebekah Juniati Setiabudi
JURNAL WIDYA MEDIKA Vol. 5 No. 2 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v5i2.2202

Indonesia is the second highest tuberculosis (TB)-burden country in the world. TB affects men and women in productive ages. Early and accurate diagnosis of TB is crucial in TB management because it allows prompt treatment with appropriate regimen. Sputum smear microscopy is the most common method used in resource-limited laboratories. The aim of this study is to assess whether adult learning method with student-centered approach can improve knowledge in laboratory techniques in microscopic TB diagnosis. It was a quasi experimental research with one group pretest and posttest design. Pretest was conducted before the training started. During the training, participants attended lectures, discussion sessions, and hands-on laboratory activities. After training, participants were asked to respond to posttest. There were 33 participants of this training (n=33). Paired t-test analysis showed significant difference between pretest and posttest scores (p=6.5x10-9). Variance between pretest scores was 4.00 and variance between posttest scores were 1.34. Pearson correlation coefficient was 0.45. Regardless their level of knowledge before training, participants could achieve relatively equivalent results after training. Significant difference between pre- and post-training test scores indicated that the learning method was effective and the training could improve participants knowledge in laboratory techniques in microscopic TB diagnosis.

In Vitro Analysis of SARS-CoV-2 Variants that Caused Severe COVID-19 in the Elderly Sutandhio, Silvia; Wihanto, Laura; Tedyanto, Cecilia Putri; Santoso, Sentot
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 1 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i1.65484

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the global problem of respiratory disease from 2019 to 2024. One of the earliest variations in the SARS-CoV-2 S protein was the S D614G mutation. SARS-CoV-2 has several important variants, namely, Alpha, Beta, Gamma, Delta, and Omicron. Omicron is the variant that has caused severe health problems, someresulting in death, in the elderly. Omicron has further differentiated to some wellknown variants, such as, BA.1, BA.2, BA.2.75, BA.5, BQ.1.1, and XBB.1. According to Japanese Government data, the number of citizens aged 65 years old and above reached 28.9% in 2021. From our previous experiment, antibodies of the elderly that have received four doses of mRNA vaccine still could not optimally neutralize Omicron BQ.1.1 and XBB.1. We aimed to analyze the plaque size of SARS-CoV-2 variants that caused severe COVID-19 in the elderly. SARS-CoV-2 variants were seeded in Vero E6-TMPRSS2 cell culture to create plaques. The resulting plaques were analyzed with ImageJ application to select solitary plaques and to determine plaque sizes. The size of BA.1 plaque was indifferent to BA.2 plaque. The plaque area comparison result was as follows, BA.1/BA.2<BA.5<BA.2.75<BQ.1.1<XBB.1. The plaque sizes of Omicron BQ.1.1 and XBB.1 were bigger that those of Omicron BA.1 and BA.2. The plaque sizes of all Omicron variants were smaller than those of the previousvariants, S D614G and Delta. The result of this in vitro experiment inferred that there is increase in fusogenicity of BQ.1.1 and XBB.1, when compared with BA.1 and BA.2.

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