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Effects of gamal leaf extract (Gliricidia sepium Jacq.) on body weight and internal organ performance of mice (Mus musculus L.) Harlis, Wa Ode; Malik, Nurhayu; Luvita, Luvita; Resman, Resman
Jurnal Pijar Mipa Vol. 18 No. 6 (2023): November 2023
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v18i6.5730

Abstract

Gamal leaves (Gliricidia sepium Jacq.) contain toxic compounds that have the potential to act as vegetable rodenticides, such as alkaloids, tannins, dicumerol, and hydrogen cyanic acid (HCN). Coumarin compounds can bind vitamin K, disrupt the circulatory system, and damage liver cells. The compounds dicumerol and warfarin are used as rat poison because of their anticoagulant properties, which prevent blood clots in the heart. Alkaloid and tannin compounds can damage liver cells through liver necrosis and bleeding in the digestive system. This study aims to determine the effect of Gamal leaf extract on body weight and internal organ performance in mice (Mus musculus L.). The experimental research method is based on a Completely Randomized Design (CRD) pattern. A total of 16 mice weighing 20-22 grams consisting of 4 treatments, namely A0 (water control), A1 (extract 10 mg/g BW), A2 (extract 15 mg/g BW) and A3 (extract 20 mg/g BW). The extract was given orally for 14 days, and body weight was measured every 3 days to obtain data on changes in body weight (PBB). At the end of the treatment, the mice were sacrificed using chloroform and then dissected to observe the performance of the liver, kidney and heart organs in color appearance and organ weight. Quantitative data were analyzed using one-way ANOVA and further DMRT test α=0.05. The research showed that mice's mean change in body weight (PBB) decreased significantly as the dose of Gamal leaf extract increased. The percentage of liver organ weight (BOH), kidney organ weight (BOG), and heart organ weight (BOJ) decreased as the dose of Gamal leaf extract increased. The research results concluded that Gamal leaf extract caused color changes in the internal organs of mice and significantly affected the percentage change in body weight (PBB) and internal organ weight (BOD) of mice along with increasing doses of Gamal leaf extract.
Effect of White Ginger Rhizome Extract (Zingiber officinale var. amarum) on Pregnancy in Mice (Mus musculus L.) Early Post-implantation Stage Harlis, Wa Ode; Salwinda, Salwinda; Malik, Nurhayu; Resman, Resman
Jurnal Pijar Mipa Vol. 19 No. 4 (2024): July 2024
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v19i4.6932

Abstract

White ginger (Zingiber officinale var. Amarum) contains compounds in the antifertility group, namely flavonoids, terpenoids, and essential oils (gingerol and shogaol). Flavonoids can interfere with zygote division and implantation, while terpenoids and essential oils disrupt sperm transport and cause sperm to clot in male animals. This study aims to determine the effect of white ginger (Zingiber officinale var. Amarum) rhizome extract on the mouse (Mus musculus L.) early post-implantation stage. This research type is experimental and structured using a Completely Randomized Design (CRD) pattern. A total of 16 female mice weighing 20-30 g aged 2-3 months were divided into four treatments, namely K1 (control), K2 (0.7 mg/g BW), K3 (1.4 mg/g BW), and K4 (2.8 mg/g BW) white ginger rhizome extract. The extract is given orally at 0.5 mL/head/day on the 4th to 8th day of pregnancy. The mother's body weight is weighed every three days. On the 18th day of pregnancy, mice were sacrificed using chloroform and dissected to observe early post-implantation pregnancy parameters. Data were analyzed using Analysis of Variance (ANOVA) and Duncan's follow-up test with a confidence level of 95%.   The research showed that white ginger rhizome extract significantly reduced the percentage of implantation (IM) and the percentage of live fetuses (FH), causing fetal growth to be hampered. The rate of implantation (IM) is K1 (1.8%), K2 (0.52%), K3 (0.49%). The percentage of live fetuses (FH) was K1 (1.06%), K2 (0.48%), and K3 (0.30%), while no fetus was found in K4. K3 treatment (1.4mg/g) causes fetal growth to be hampered. White ginger rhizome extract, on average, increases post-implantation mortality (KPI) and percentage of embryo resorption (ER) and reduces fetal weight. Based on the results of this study, it is concluded that white ginger extract has antifertility properties in female animals because it causes pregnancy disorders, so it is hoped that the use of white ginger in early pregnancy will be reduced.
Pengaruh Fungi Mikoriza Arbuskula dan Jarak Tanam terhadap Pertumbuhan Tanaman Kacang Hijau (Vigna radiata L.) Halim, Halim; Sabaruddin, Laode; Arma, Makmur Jaya; Resman, Resman; Hisein, Waode Siti Anima
Jurnal Penelitian Inovatif Vol 4 No 2 (2024): JUPIN Mei 2024
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jupin.389

Abstract

Produksi kacang hijau di Sulawesi Tenggara selalu mengalami fluktuasi yang disebabakan oleh kondisi iklim yang berubah-ubah, kesuburan tanah yang rendah serta sistem budidaya yang masih konvensional. Untuk meningkatkan produksi kacang hijau, maka perlu adanya budidaya tanaman yang baik dengan input yang mudah diterapkan oleh petani. Penelitian ini bertujuan untuk mengetahui pengaruh fungi mikoriza arbuskula dan jarak tanam terhadap pertumbuhan tanaman kacang hijau. Penelitian ini dilaksanakan di Laboratorium Lapangan Kebun Percobaan II Fakultas Pertanian Universitas Halu Oleo Kendari. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) pola factorial yang terdiri dari dua factor yaitu: faktor pertama propagul fungi mikoriza arbuskula (FMA) yang terdiri atas empat taraf dan Faktor kedua yaitu jarak tanam yang terdiri atas tiga taraf. Variabel yang diamati yaitu: tinggi tanaman (cm), jumlah daun (helai), diameter batang (cm), luas daun (cm2). Hasil penelitian menunjukkan bahwa terdapat interaksi antara fungi mikoriza arbuskula dengan jarak tanam terhadap tinggi tanaman 21, 28 dan 35 HST, jumlah daun 28 HST, diameter batang 35 HST dan luas daun 21, 35 dan 42 HST.