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Pengaruh Infeksi Trypanosoma evansi terhadap Kadar TNF-α dan Perubahan Histopatologi Hepar pada Tikus Putih (Rattus norvegicus) Kurnianto, Ady; Pratama, Junianto Wika Adi; Candrarisna, Miranti
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 8, No 1 (2019): EDISI MARET 2019 (available online since April 2019)
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (602.039 KB) | DOI: 10.30742/jikw.v8i1.548

Abstract

Tujuan penelitian ini adalah untuk menganalisis kadar Tumor necrosis Factor ?(TNF-?) dan derajat kerusakan hepar pada tikus putih (Rattus norvegicus) yang diinfeksi isolat Trypanosoma evansi. Penelitian ini adalah penelitian eksperimental dengan Rancangan Acak Lengkap (RAL). Sampel Hewan adalah tikus jantan umur 6 minggu sebanyak 30 ekor dan dibagi dalam 5 kelompok dengan pemberian infeksi secara subcutan (sc), yaitu Po: kelompok tikus kontrol diinjeksi NaCl fisiologis dosis 0,3 ml, pengambilan sampel darah dan organ hepar pada hari ke 7 pasca injeksi NaCl fisiologis. P1: kelompok tikus diinfeksi Trypanosoma evansi dosis 0,3 ml/sc, pengambilan sampel darah dan organ hepar pada hari pertama pasca infeksi Trypanosoma evansi. P2: kelompok tikus diinfeksi Trypanosoma evansi dosis 0,3 ml/sc, pengambilan sampel darah dan organ hepar pada hari ke 3 pasca infeksi Trypanosoma evansi. P3: kelompok tikus diinfeksi Trypanosoma evansi dosis: 0,3 ml/sc, pengambilan sampel darah dan organ hepar pada hari ke 5 pasca infeksi Trypanosoma evansi. P4: kelompok tikus putih diinfeksi Trypanosoma evansi dosis 0,3 ml/sc, pengambilan sampel darah dan organ hepar pada hari ke 7 pasca infeksi Trypanosoma evansi. Nilai Optical Density (OD) atau kadarTNF-?menunjukkan p=0,0624 (p>0,05), mengalami penurunan dan tidak berbeda, dan tidak terdapat hubungan bermakna antara kelompok pada tikus putih yang diinfeksi Trypanosoma evansi isolat Sumbawa. Pemberian infeksi secara subkutan dapat menyebabkan kerusakan hepar berupa lesi degenerasi, nekrosis, dan portal inflamasi pada tikus putih yang diinfeksi Trypanosoma evansi isolat Sumbawa. Kesimpulan adalah kadar TNF-? menurun, mengakibatkan kerusakan hepar dan tingkat keganasan parasit Trypanosoma evansi isolat Sumbawa meningkat.
Aquaria Flushing Technique Of Rosella Extract (Hibiscus sabdariffa Linn) to Determine The Value of SGOT and SGPT in Milk Fish (Chanos chanos) Induced by Heavy Metals (Hg) Astuti Nussa, Olan Rahayu Puji; Hermawan, Intan Permatasari; Kurnianto, Ady
Agrikan Jurnal Agribisnis Perikanan Vol. 15 No. 2 (2022): Agrikan: Jurnal Agribisnis Perikanan
Publisher : Fakultas Pertanian, Universitas Muhammadiyah Maluku Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52046/agrikan.v15i2.1358

Abstract

The purpose of this study was to determine the value of SGOT and SGPT in milkfish induced by heavy metals (Hg) by aquaria flushing technique with Rosella (Hibiscus Sabdariffa Linn) extract. The samples used were 30 male adult milkfish divided into 5 groups. The treatment group was induced with a toxic dose of heavy metal mercury (Hg), which was dissolved in a volume of m3 of water. Blood collection from the heart vein of milkfish as much as 1-2 ml for SGPT and SGOT examination was carried out using the Berthelot method. The liver, gills and brain of milkfish were then examined, 300 grams were centrifuged until smooth and then dissolved in methanol to obtain concentrations of 25ppm, 50ppm, 100ppm, 200ppm, 300ppm, 400ppm, 500ppm, 600ppm, 700ppm, 800ppm, 9 00ppm. and 1000ppm then analyzed by ANOVA. Based on the research results of black roselle flower extract therapy with aquaria flushing system on male milkfish, the average value of SGOT and SGPT showed a very significant decrease in groups P1, P2, P3, and P4, against the P0 group or control group (P<0, 01).
Studi Kasus: Terapi Mastitis Klinis pada Sapi Perah di Kota Batu Hermawan, Intan Permatasari; Kurnianto, Ady; Kartikasari, Dian Ayu; Pratama, Junianto Wika Adi; Astuti, Mery
JURNAL KAJIAN VETERINER Vol 12 No 1 (2024): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v12i1.8192

Abstract

The purpose of this case report is to report a case of clinical mastitis in dairy cattle in Batu. One case was reported on this period. Anamnesis was carried out, the owner revealed that the cow did not produce milk and when milking the udderand nipples felt hard and the milk that wanted to come out seemed to be stuck. Physical examination was carried out, cow’s weight ±400 kg, body temperature 38.4 °C, the udder and nipple were swollen and inflamed, and decrease of milk production, mammae showed reddish and asymmetrical and painful when palpate. Based on the history and physical examination of the cow, it can be concluded that the cow was diagnosed with clinical mastitis with a fausta prognosis and the differential diagnosis was mammary tumour. The therapy given in this case was injection of the antibioticInterspectin-L® 10 ml, the anti-inflammatory and antipyretic Tolfedine® 10 ml intramuscularly.
Laporan Kasus : Feline Immunodeficiency Virus pada Kucing Moi di Surabaya Kurnianto, Ady; Millenia, Maria
JURNAL KAJIAN VETERINER Vol 11 No 2 (2023): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v11i2.12803

Abstract

Feline immunodeficiency (FIV) is a disease caused by retroviridae of the lentivirus genus and is dangerous for cats. This virus attacks the cat's immune system. FIV disease can be transmitted horizontally via as saliva and body fluids. 5-month-old black and white Persian cat named Moi, brought by the owner with complaints of vomiting yesterday, refusing to eat and drink, soft stool, no coughing and sneezing, weakness. On physical examination showed a temperature of 40.1 °C with a body weight of 1.5 kg, clinical symptoms were found in the form of gingivitis, canker sores, conjunctivitis, pale pink mucous membranes, fever, flu symptoms from the nose with clear discharge, crepitus in both ears. Diagnosis of Moi Cats based on anamnesis, physical examination, clinical symptoms, and supporting examinations is diagnosed with Feline immunodeficiency virus (FIV). Case are treated using a combination of antibiotics, anti-inflammatories, fluid therapy, antiemetics, gastrointestinal linings, immune boosters, blood boosters, and cardiac pacemakers. After 4 days the cat Moi was declared dead due to failure of platelet hemostasis, bleeding, and heart failure.
EMPOWERING WOMEN OF BHAYANGKARI RANTING DUKUH PAKIS THROUGH TRAINING IN MANUFACTURING FUNCTIONAL EDIBLE BIRDS' NEST DRINK WITH LOCAL FLAVOR Gusti Ningrum, Siti; Rahayu Puji Astuti Nussa, Olan; Kurnianto, Ady
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 8, No 7 (2025): MARTABE : JURNAL PENGABDIAN KEPADA MASYARAKAT
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v8i7.2876-2882

Abstract

Sarang burung walet (SBW) adalah minuman fungsional yang memiliki banyak manfaat sebagai anti inflamasi dan antiviral. Potensi minuman fungsional SBW telah diteliti sebelumnya dengan menggunakan teknik in silico. Pada pengabdian kepada masyarakat kali ini, teknik pembuatan minuman SBW ini dilakukan sebagai lanjutan dari Program Penelitian Sains 2021. Inovasi ini telah memberikan banyak manfaat kesehatan bagi masyarakat yang rutin mengkonsumsi SBW ini. Metode pembuatan minuman fungsional SBW menggabungkan dua bahan lokal asli Indonesia yaitu cengkeh dan kayumanis. Dengan inovasi ini akan menambah nilai komersial karena rasa lokalnya yang diterima baik oleh masyarakat. Tujuan pengabdian kepada Masyarakat ini adalah untuk melatih ibu-ibu Bhayangkari Cabang Dukuh Pakis dalam pembuatan minuman fungsional SBW. Setelah survei ke Kantor Polisi Cabang Dukuh Pakis, penyampaian materi dilakukan dengan bahan materi meliputi produksi minuman SBW, pengemasan, pengurusan ijin produksi, dan pemasaran. Hasil pengabdian kepada masyarakat menunjukkan keberhasilan ibu-ibu Bhayangkari Cabang Dukuh Pakis dalam pembuatan minuman SBW. Selain itu, kegiatan ini diharapkan mampu meningkatkan perekonomian rumah tangga dan memperkuat komunitas ibu-ibu Bhayangkari Cabang Dukuh Pakis serta berkontribusi meningkatkan kesehatan masyarakat. 
Pengaruh Sari Buah Nanas (Ananas Cosumus l.) Terhadap Total Plate Count (TPC) Dan Derajat Keasaman (pH) Daging Ayam Broiler Widhowati, Dyah; Wiranata, Ferian Pandu; Kurnianto, Ady; Yanestria, Sheila Marty
VITEK : Bidang Kedokteran Hewan Vol 11 No 2 (2021): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v11i2.85

Abstract

The aimed of this study was to determine the effectivity of pineapple essence (Ananas Comosus) as a natural reservative in chicken meat with parameters of total colony of bacteria and pH. The experimental design used completely randomized design with 4 treatments and 6 replications. The treatments applied were P0 as a control, P1 (25% pineapple essence), P2 (50% pineapple essence), and P3 (100% pineapple essence). The parameters of this study were the total colony of bacteria (TPC) and pH. The results of the statistical analysis showed that the total colony of bacteria in meat were P0 = 4,2 x104, P1 = 2,0 x 104, P2 = 1,9 x 104, dan P3 = 1,2 x 104. pH in chicken meat P0 = 5,0 ± 0,17a, P1 = 4,2 ± 0,05b, P2 = 4,0 ± 0,08c, dan P3 = 3,9 ± 0,10d.. The results of this study showed significant difference in chicken meat containing pineapple essence on total colony of bacteria and pH. The most effective result was chicken meat with 100% pineapple essence.
Efektivitas Campuran Ekstrak Daun Kelor (Moringa oleifera) dan Serai Wangi (Cymbopogon nardus l) Terhadap Mortalitas Larva Nyamuk Aedes aegypti Hidayah, Nurul; Kurnianto, Ady; Bhelo, Angela; Palgunadi, Bagus Uda
VITEK : Bidang Kedokteran Hewan Vol 11 No 2 (2021): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v11i2.86

Abstract

This study aimed to determine the effectiveness of a mixture of Moringa leaf extracts (Moringa oleifera) and citronella (Cymbopogon nardus L)on the mortality of mosquito larvae Aedes aegypti. Experimental animals used in this study mosquito larvae Aedes aegypti were 625. The design used was a completely randomized design with 5 treatments and 5 replications. The five treatments were the treatment group consisting of concentrations of 2%, 4% and 6% and the control group consisting of positive control, namely abate and negative control, namely aquades. Mosquito larvae mortality was observed every 2 hours for 12 hours. The data obtained were analyzed using one way ANOVA test and continued with Duncan's test. The results obtained in the mixed treatment of Moringa leaf extract and lemongrass 4% and 6% had high larvicidal ability and were the same as positive control themepos (abate). Based on the results of the study, it was concluded that a mixture of Moringa leaf extracts (Moringa oleifera) and citronella (Cymbopogon nardus L)had larvicidal ability against mosquito larvae Aedes aegypti.
Efektivitas Antibakteri Ekstrak Daun Sisik Naga (Drymoglossum piloselloides) Terhadap Bakteri MRSA (methicilin resistant Staphylococcus aureus) dan Eschericia coli Wirjatmadja, Roeswandono; Kurniasari, Putri Nadia Indah; Wibisono, Freshinta Jellia; Kurnianto, Ady
VITEK : Bidang Kedokteran Hewan Vol 12 No 2 (2022): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v12i2.112

Abstract

The purpose of this research aimed to determine the antibacterial effectiveness of the methanolic extract of dragon scales leaves (Drymoglossum piloselloides) against MRSA (Methicilin-resistant Staphylococcus aureus) and Eschericia coli bacteria in vitro using the diffusion method. This research used a population of Methicillin-resistant Staphylococcus aureus and Eschericia coli bacteria. The sample to be used is dragon scale leaf methanol extract. The method in this research used experimental with 6 treatments, namely dragon scale leaf methanol extract with concentrations of 45%, 55%, 65%, 75%, negative control (methanol) and positive control (chloramphenicol antibiotics). Data collection in this study was carried out by recording the diameter of the inhibition zone using a caliper (mm). The data obtained from the diameter of the inhibition zone of 75% extract variation resulted in an inhibition zone of 17.02 mm (in MRSA) and 8.47 mm (in E.coli). Chloramphenicol was found to be resistant to MRSA, but sensitive to E.coli. Data analysis used ANOVA test which statistically showed that there was a significant difference (P<0.05) in the diameter of the inhibition zone of the dragon scale leaf methanol extract against MRSA bacteria and there was a very significant difference (P<0.01) in the diameter Inhibition zone of dragon scale leaf methanol extract against Eschericia coli bacteria.
Studi kasus : Feline Panleukopenia Virus pada Kucing Abel Albab, Ulul; Febrianth, Alvin; Hermawan, Intan Permatasari; Kurnianto, Ady
VITEK : Bidang Kedokteran Hewan Vol 12 No 2 (2022): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v12i2.113

Abstract

A domestic cat with short hair, approximately three months old, was brought to the Tabby Pet Care clinic with complaints that the cat's owner had vomiting and diarrhea. The results of clinical examination showed a state of dehydration and swollen popliteal. Supportive examinations include hematological examinations and rapid test kits. Hematological examination results showed anemia and leukocytosis. The results of the rapid test kit examination showed a positive FPV result. Based on clinical symptoms and the results of investigations, the patient was diagnosed as positive for Feline Panleukopenia Virus. Treatment of FPV is carried out with supportive therapy. Therapy was carried out for 7 days. On the seventh day, based on the results of the hematological examination and the patient's body condition, his condition progressed and he was allowed to continue outpatient therapy.
A Studi Kasus: Indigesti Sederhana pada Sapi Limousin di KUD Karya Bhakti, Ngancar, Kediri Ningsih, Awalul Fitria; Kurnianto, Ady
VITEK : Bidang Kedokteran Hewan Vol 14 No 1 (2024): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v14i1.225

Abstract

Indigestion is a syndrome of indigestion originating from the rumen or reticulum, characterized by decreased or lost movement of the rumen, weak tone of the two stomachs, indesgeta buried in it and accompanied by constipation. An indigestive case in a limousine cow named Beno, male, 12 months old, 400 kg body weight, brown hair color, was physically examined by inspection and palpation. The results of the physical examination by inspection were weakness and lethargy, hypersalivation, hyperlacrimation, slightly reddish conjunctiva of the eyes, slightly pale mucous membranes of the mouth, nose and ears and the earlobes felt cold, no lesions on the skin and hair, no lice, slightly dull hair. The results of palpation of the lymph nodes did not experience swelling. Palpation of the left abdomen was distended (the ventral part of the rumen/left paralumbar fossa felt tense, rumen movement was slow), a small amount of feces was found and the consistency was slightly runny, the frequency of urine was small. Normal body temperature is 41.1oC, abnormal respiratory rate is 49/minute. Treatment is in the form of drug therapy including injection of analgesics and antipyretics, anti-inflammatories, antihistamines, antibiotics, and multivitamins. Keywords: Simple indigestion, cow, inspection, palpation.