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Pengaruh Infeksi Trypanosoma evansi terhadap Kadar TNF-α dan Perubahan Histopatologi Hepar pada Tikus Putih (Rattus norvegicus) Kurnianto, Ady; Pratama, Junianto Wika Adi; Candrarisna, Miranti
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 8, No 1 (2019): EDISI MARET 2019 (available online since April 2019)
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (602.039 KB) | DOI: 10.30742/jikw.v8i1.548

Abstract

Tujuan penelitian ini adalah untuk menganalisis kadar Tumor necrosis Factor ?(TNF-?) dan derajat kerusakan hepar pada tikus putih (Rattus norvegicus) yang diinfeksi isolat Trypanosoma evansi. Penelitian ini adalah penelitian eksperimental dengan Rancangan Acak Lengkap (RAL). Sampel Hewan adalah tikus jantan umur 6 minggu sebanyak 30 ekor dan dibagi dalam 5 kelompok dengan pemberian infeksi secara subcutan (sc), yaitu Po: kelompok tikus kontrol diinjeksi NaCl fisiologis dosis 0,3 ml, pengambilan sampel darah dan organ hepar pada hari ke 7 pasca injeksi NaCl fisiologis. P1: kelompok tikus diinfeksi Trypanosoma evansi dosis 0,3 ml/sc, pengambilan sampel darah dan organ hepar pada hari pertama pasca infeksi Trypanosoma evansi. P2: kelompok tikus diinfeksi Trypanosoma evansi dosis 0,3 ml/sc, pengambilan sampel darah dan organ hepar pada hari ke 3 pasca infeksi Trypanosoma evansi. P3: kelompok tikus diinfeksi Trypanosoma evansi dosis: 0,3 ml/sc, pengambilan sampel darah dan organ hepar pada hari ke 5 pasca infeksi Trypanosoma evansi. P4: kelompok tikus putih diinfeksi Trypanosoma evansi dosis 0,3 ml/sc, pengambilan sampel darah dan organ hepar pada hari ke 7 pasca infeksi Trypanosoma evansi. Nilai Optical Density (OD) atau kadarTNF-?menunjukkan p=0,0624 (p>0,05), mengalami penurunan dan tidak berbeda, dan tidak terdapat hubungan bermakna antara kelompok pada tikus putih yang diinfeksi Trypanosoma evansi isolat Sumbawa. Pemberian infeksi secara subkutan dapat menyebabkan kerusakan hepar berupa lesi degenerasi, nekrosis, dan portal inflamasi pada tikus putih yang diinfeksi Trypanosoma evansi isolat Sumbawa. Kesimpulan adalah kadar TNF-? menurun, mengakibatkan kerusakan hepar dan tingkat keganasan parasit Trypanosoma evansi isolat Sumbawa meningkat.
Lung Change Pattern On Domestic Cat (Felis Silvestris Catus) Exposed By Factory Air Pollution With X-Ray Thorax Interpretation Ratna Widyawati; Desty Apritya; Junianto Wika Adi Pratama; Asnizar Fahmi
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 4 No. 2, JULY 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v4i2.10697

Abstract

The pathology density changes for X-Ray interpretation of the lungs are difficult to distinguish, because the  disease is not easily classified and may represent a variety of lung disorders. The study was aimed of this research to found the lung disease of domestic cats (Felis Silvestris Catus) that exposure to air pollutions in the industrial area of region Manyar, district Gresik. The type of this research was observational study Explorative that using the simple random sampling and observed nine cats for the sampling.The results of X-ray Thorax in this research showed that there are changing in the 3rd, 4th, 5th, 6th, 7th, an 9th of the sampling cats. On the third, fifth and the seventh of the cats, we found the same condition that there was change the vascular patterns that were enlargement, vascular constriction or ambiguity structures. From the results of the vascular patern indicate the disorder of left-sided heart failure (Mitral Isufisiency) or obstruction of the left atrium. On the fourth of the cat, there was bronchial pattern that showed the classification, dilatation and thickness of the lung. On the sixth of the cats, there were changes on interstitial pattern with the pictures of nodulars that were not structures and on the ninth of the cats, we can found the same conditions with the fourth and the sixth of cats were visible Interstitial Pattern and Bronchial Interstitial Pattern.
EFEKTIVITAS PENGASAPAN MENGGUNAKAN KAYU KESAMBI (Schleichera oleosa) DIBANDINGKAN DENGAN BATOK KELAPA (Cocos nucifera) PADA DAGING BABI TERHADAP TPC, ORGANOLEPTIC DAN AWAL PEMBUSUKAN Sheila Marty Yanestra; Junianto Wika Adi Pratama; Asih Rahayu; Patrianus Tomy
VITEK : Bidang Kedokteran Hewan Vol 10 (2020): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v10i0.46

Abstract

The study was conducted to determine the effect of fogging using kesambi wood(Schleichera Oleosa) compared to coconut shells (Cocos Nucifera) in pork on TPC, organolepticsand early decay. The experimental design used was a completely randomized design with fourtreatments with a total of six replications. The treatments applied were P1 fogging for 30 minutesusing wood kesambi (Scheleichera Oleosa), P2 fogging for 30 minutes using coconut shells (CocosNicifera), P3 fogging for 45 minutes using kesambi wood (Scheleichera Oleosa), P4 fogging for 45minutes using coconut shells (Cocos Nicifera), P3 fogging for 45 minutes using wood cage(Scheleichera Oleosa), P4 fogging for 45 minutes using coconut shells (Cocos Nicifera), P3 foggingfor 45 minutes using wood cage (Scheleichera Oleosa), P4 fogging for 45 minutes using coconutshells (Cocos Nicifera), P3 fogging for 45 minutes using wood cage (Scheleichera Oleosa) coconut(Cocos Nucifera). The results of the analysis of total plate count (TPC) showed that there was noreal difference. The results of the color and taste organleptic analysis showed that there was nosignificant difference between P1, P2, P3 and P4 where P ˃ 0.05, while the texture and aroma testshowed significant changes between P1, P2, P3 and P4 where P ˂ 0.05. In the initial decay testshowed that p1 had the lowest positive results of the initial decay test that is 1 positive sample froma total of 6 samples.
EFEKTIVITAS PERENDAMAN LARUTAN EKSTRAK DAUN BABADOTAN (Ageratum conyzoides L) DENGAN KOSENTRASI BERBEDA PADA KUALITAS TELUR AYAM RAS DALAM SUHU RUANGAN bagus uda palgunadi; Indra Rahmawati; Junianto Wika Adi Pratama; Mohammad Safrizal Alam Hudi
VITEK : Bidang Kedokteran Hewan Vol 9 (2019): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v9i0.63

Abstract

In order to investigate the effect of immersion babadotan leaf extract solution (Ageratum conyzoides L) with various centration of the quality of chicken eggs in room temperature. Reviewed from the yolk index and haugh unit. The sample are 1 day chicken eggs race with an average weight of 50-60 grams, which is taken from PT. Mulya Broiler Farm, Binangun village, Binangun subdistrict, Blitar regency. It used as much as 100 eggs, with immersion treatment using Babadotan leaf extract solution for 1 minute with the centralization of the solution 30%, 40%, 50% and 60%, and stored in room temperature, than calculated the value of yolk index and haugh unit. Results of the study showed that immersion by using Babadotan leaf extract solution had no noticeable effect (P > 0.05) against the Haugh and very noticeable effect (P < 0.05) on the value of yolk Index. Quality of chicken eggs are given the immersion babadotan leaf extract solution is better than without immersion.
PENGARUH BEBERAPA METODE THAWING TERHADAP KUALITAS SEMEN BEKU SAPI SIMENTAL Junianto Wika Adi Pratama; Dian Ayu Kartika Sari; Miarsono Sigit
Jurnal Ilmiah Fillia Cendekia Vol 3 No 2 (2018): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.658 KB) | DOI: 10.32503/fillia.v3i2.254

Abstract

The study aimed to determine the effect of several temperatures of thawing water and the length of thawing on the quality of Simmental frozen semen. This research method was an experimental study used a multifactorized completely randomized design with treatment of thawing water temperature 26oC, 37oC, 42oC and long thawing 10 seconds, 15 seconds and 20 seconds. The data obtained were analyzed by Analysis of Variance (ANOVA). If there are differences in the real or very significant influence on each treatment, then Duncan's multiple distance test is continued. The results showed that there was a significant effect (P <0.05) between the duration of thawing and thawing temperature on sperm motility and viability, but there was no interaction between temperature and thawing time (P> 0.05). Thawing temperature of 37 ° C and thawing time of 20 seconds showed the highest viability and motility of sperm so that it could provide the best quality of Simmental cow frozen semen for artificial insemination
Perbandingan Kadar Lemak, Protein Dan Bahan Kering Tanpa Lemak (BKTL) Pada Susu Sapi Segar Di Kota Kediri Dan Kabupaten Kediri Miarsono Sigit; Wahyu Rafida Putri; Junianto Wika Adi Pratama
Jurnal Ilmiah Fillia Cendekia Vol 6 No 1 (2021): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v6i1.1401

Abstract

The aimed of the study to determine the difference fat content, protein content and Solid Non Fat (SNF) in fresh cow’s milk in Kediri City and Kediri District. This study used fresh cow's milk from Friesian Holstein crossbreed as many as 40 samples, 20 samples from Kediri City and 20 samples from Kediri District. The type of research was a survey with a quantitative approach. Sampling was carried out used quota sampling by comparing the fat content, protein content and Solid Non Fat (SNF) in fresh cow's milk from Kediri City and Kediri District. The data analyzed by T-test. After testing milk using Lactoscan then calculated and found the average fat content from the City of Kediri was 2.9525 mg / dl and the averaged fat content from Kediri District was 3.2860 mg/dl. The averaged of the protein content from Kediri City was 2.1025 mg/dl and the protein content from Kediri Distict 2.1795 mg/dl. The average Solid Non Fat (SNF) from the City of Kediri is 5.6620 mg/dl and the averaged Solid Non Fat (SNF) from Kediri Regency is 5.8690 mg/dl. The result showed that no significant difference (P>0,05) in fat content, protein content and Solid Non Fat (SNF).
Korelasi Antara Volume Skrotum Terhadap Motilitas , Viabilitas Dan Konsentrasi Spermatozoa Sapi Pejantan Peranakan Ongole Miarsono Sigit; Junianto Wika Adi Pratama; Hana Cipka Pramuda Wardhani; Arief Mardijanto
Jurnal Ilmiah Fillia Cendekia Vol 7 No 1 (2022): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v7i1.2277

Abstract

Penelitian ini bertujuan untuk mengetahui hubungan antara panjang dan lingkar skrotum terhadap konsentrasi, motilitas dan viabilitas sperma pejantan sapi Peranakan Ongole (bos indicus). Sampel yang digunakan sebanyak 10 ekor sapi Peranakan Ongole dengan usia rata rata lima sampai sepuluh tahun. Variabel yang diamati adalah lingkar skrotum, panjang skrotum, konsentrasi sperma, motilitas sperma ,dan viabilitas sperma. Data analisis menggunakan korelasi dan analisis regresi linear sederhana. Hasil penelitian ini menunjukan korelasinya antara volume skrotum dengan konsentrasi spermatozoa r = 0,0662, viabilitas sperma r = 0,462, motilitas sperma r = 0,175, sehingga terdapat hubungan sedang antara volume skrotum dengan konsentrasi sperma, dan tidak terdapat hubungan antara volume skrotum dengan viabilitas sperma dan motilitas sperma sapi Peranakan Ongole.
PEMERIKSAAN GOLONGAN DARAH DAN TRANSFUSI DARAH PADA KUCING DAN ANJING SECARA GRATIS DI RUMAH SAKIT HEWAN PENDIDIKAN WEKA FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS WIJAYA KUSUMA SURABAYA Era Hari Mudji Restijono; Retina Yunani; Palestin Palestin; Hana Cipka Pramuda Wardhani; Desty Apritya; Kurnia Desiandura; Dian Ayu Kartika Sari; Muhammad Noor Rahman; Ady Kurnianto; Junianto Wika Adi Pratama; Intan Permatasari Hermawan
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 5, No 9 (2022): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v5i9.3314-3319

Abstract

Pemeriksaan golongan darah kucing merupakan hal penting sebagai salah satu screening awal jika ingin melakukan transfusi darah pada kucing. Transfusi darah dilakukan pada pasien yang membutuhkan atau dalam keadaan darurat seperti anemia berat, infeksi parasit darah, atau dalam keadaan traumatik yang menyebabkan adanya pendarahan hebat. Transfusi darah dapat meningkatkan prosentase keselamatan pasien yang menderita kejadian tersebut. Pada saat transfusi darah, wajib menyiapkan pendonor dan resipien yang sudah terseleksi dengan ketat, antara lain dalam keadaan sehat, bebas parasit darah, bebas infeksi Feline Immunodeficiency Virus dan Feline Leukimia Virus, dan indoor life.
PENINGKATAN SUMBER DAYA EKONOMI PETERNAK KELINCI DI KEDIRI MELALUI PERBAIKAN MANAJEMEN PAKAN DAN PRODUKSI Miarsono Sigit; Junianto Wika Adi Pratama; Hana Cipka Pramuda Wardhani; Ferdi Nur Ardiansyah
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 5, No 7 (2022): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v5i7.2634-2645

Abstract

Kediri is a densely populated city that has problems regarding livestock. The problems that are often experienced are the land for raising livestock and the availability of feed. Raising rabbits is one of the ways used by the Sumber Rejeki livestock group in Kediri. Rabbits can digest various types of feed, so that it can facilitate the maintenance process, especially in the Kediri area which utilizes local feed resources. Rabbits have good potential and can be superior in terms of reproduction and production. The hope is that with rabbit cultivation, group members will be able to improve the economy and nutritional value of the family or the wider community. Constraints that are often experienced by rabbit breeders are the number of puppies that are relatively low in each period, the mortality rate is quite high in puppies and adult rabbits due to frequent bloating and diarrhea, and the lack of information about the local waste that can be used as feed for rabbits. Lack of knowledge in preparing rations for rabbits at each different phase, and lack of knowledge about online marketing. The solution that is expected is to carry out training on proper rabbit cultivation, pregnancy checks, counseling on handling forage, and training members of livestock groups to prepare rabbit rations. Additional provision of complete feed ingredients for rabbits supplemented with cabbage leaves, moringa leaves, and sengon leaves as well as pollard, rice bran, copra meal, soybean meal, drops, and mineral salts. And don't forget to teach marketing of their products in the form of rabbits and biscuits for rabbits online in electronic media and market places. The resulting outputs include increasing the number of children in the next period, reducing the mortality rate of rabbits, and making biscuit-shaped feed so that it is easier to bite and can be consumed by other rodents. This feed product will later be referred to as rabbit bio supplement biscuits. Making rations for weaning rabbits and brooders.
Identifikasi Bakteri Penyebab Kasus Saluran Pernapasan pada Ayam Buras di Pasar Wonokromo Surabaya Wardhani, Lailia Dwi Kusuma; Sigit, Miarsono; Pratama, Junianto Wika Adi; Daniel, Daniel
Jurnal Ilmiah Fillia Cendekia Vol 8 No 2 (2023): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v8i2.3809

Abstract

Studi ini bertujuan untuk mengidentifikasi bakteri penyebab kasus saluran pernapasan pada ayam buras. Ayam Buras didapatkan dari pasar Wonokromo Surabaya. Sampel ayam memiliki gejala klinis yaitu terdapat pembesaran kepala, kepala ayam sedikit miring dan tertunduk, kondisi tubuh tampak lemas, keluar cairan pada mata dan mulut Sampel ayam dilakukan nekropsi dan mengalami perubahan patologi anatomi pada organ paru tampak pucat. Isolasi dan identifikasi bakteri saluran pernapasan dilakukan swab cairan oedema pada kepala, discharge pada hidung, dan usapan organ paru. Uji isolasi primer dan sekunder dilakukan dengan cara menggores hasil swab ke media isolasi (NA,BA, MCA). Hasil uji isolasi didapatkan 3 koloni bakteri. Uji mikroskopik dengan pewarnaan gram didapatkan hasil koloni 3 yang menjadi dugaan bakteri penyebab. Koloni bakter 3 dilanjutkan uji identifikasi. Dari hasil uji isolasi primer dan sekunder, uji mikroskopik dan uji identifikasi didapatkan bakteri penyebab kasus adalah Pseudomonas aeruginosa. Kata Kunci : Identifikasi Bakteri, Kasus Saluran Pernapasan, Ayam Buras