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All Journal Majalah Kedokteran Bandung Global Medical and Health Communication
Nafik, Said
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Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Cytotoxicity of Combination Doxorubicin and Garcinia picrorrhiza Fruit Extract on Fibroblast Cell Utami, Sri; Endrini, Susi; Batubara, Lilian; Rahmah, Nunung Ainur; Syarif, Irfan; Nafik, Said; Arrahmani, Betharie Cendera; Novianto, Agung; Kusuma, Hanna Sari Widya; Widowati, Wahyu
Global Medical & Health Communication (GMHC) Vol 11, No 3 (2023)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v11i3.10985

Abstract

Combining chemotherapeutic agents such as doxorubicin with herbal products or other compounds that can enhance cytotoxicity without side effects is required. Thus, we aimed to observe the cytotoxicity of doxorubicin and sesoot (Garcinia picrorrhiza) fruit ethanolic extract (GpKar) on human fibroblast cells, BJ. This study used a post-test-only control randomized group design with n=3 and a number group of 5. The method used in this research is cell number, and viability was measured with (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. Treatments consisted of a combination of doxorubicin (0.02 μg/ml) and GpKar of 66.47 µg/ml (DES1), 132.94 µg/ml (DES2) and 265.89 μg/ml (DES3). The data were analyzed using a one-way ANOVA and Duncan post hoc tests. DES3 showed the lowest viability among treatments (89.32%). DES1 and DES2 showed high viability (>90%), 97.93%, and 95.08%, respectively. Thus, the combination of doxorubicin (0.02 μg/ml) and GpKar (66.47 µg/ml) was considered safe for further use in the following assay. In summary, the combination of doxorubicin and GpKar showed high viability in normal fibroblast cells.
Antioxidant and Antiaging Properties of Ethanolic Ripe Sesoot Fruit Extract Utami, Sri; Sosiawan, Insan; Nurul, Dewi; Purnamasari, Endah; Batubara, Lilian; Sachrowardi, Qomariyah Romadhiyani; Damayanti, Ndaru Andri; Aryenti, Aryenti; Dewi, Intan Keumala; Nafik, Said; Arrahmani, Betharie Cendera; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Utomo, Herry S
Majalah Kedokteran Bandung Vol 56, No 3 (2024)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v56.3472

Abstract

Skin aging can be characterized by changes in skin, such as the appearance of wrinkles and loss of skin moisture. Some elements that might lead to cell damages and aging are free radicals through the increase of the activities of hyaluronidase and tyrosinase. Bioactive compounds found in sesoot (Garcinia picrorhiza Miq.) are known for their antioxidant properties, which may reduce excessive amounts of free radicals in the body and act as an antiaging agent by inhibiting hyaluronidase and tyrosinase activities. This study aimed to examine the antioxidant, anti-hyaluronidase, and anti-tyrosinase properties found in Ethanolic Extract Ripe Sesoot (EERS). The ethanol extract of ripe sesoot fruit obtained from LIPI, Bogor Botanical Garden was used for the antioxidant and antiaging assays conducted at PT Aretha Medika Utama in December 2016. The activity of antioxidants was measured as the Ferric Reducing Antioxidant Power (FRAP) of the EERS, while the antiaging assays were performed through the inhibition activity of hyaluronidase and tyrosinase. Results indicated that the EERS has a higher FRAP activity (17.58 μM Fe (II)/μg) than xanthone (2.54 μM Fe (II)/μg) at the highest concentration of sample of 5,000 µg/mL. The anti-hyaluronidase of the EERS exhibited lower activity (IC50 of 619.21±12.15 µg/mL) than xanthone (IC50 of 365.55±25.10 µg/mL) and the tyrosinase inhibitory assay demonstrated a lower activity of EERS (IC50 of 1060.68±12.81 µg/mL) compared to xanthone (IC50 of 218.33±9.73 µg/mL). To conclude, EERS shows antioxidant and antiaging properties.
Co-Authors Agung Novianto, Agung Arrahmani, Betharie Cendera Aryenti, Aryenti Batubara, Lilian Damayanti, Ndaru Andri Dewi Nurul, Dewi Dewi, Intan Keumala Endah Purnamasari Hanna Sari Widya Kusuma, Hanna Sari Widya Irfan Syarif, Irfan Nunung Ainur Rahmah Sachrowardi, Qomariyah Romadhiyani Sosiawan, Insan Sri Utami Susi Endrini Utomo, Herry S WAHYU WIDOWATI