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All Journal International Journal of Power Electronics and Drive Systems (IJPEDS) Jurnal Dedikasi Dinamik Transmisi: Jurnal Ilmiah Teknik Elektro Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Medical Laboratory Technology Journal AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat JURNAL MEDIA INFORMATIKA BUDIDARMA JPM (Jurnal Pemberdayaan Masyarakat) BERDIKARI : Jurnal Inovasi dan Penerapan Ipteks Buletin Ilmiah Sarjana Teknik Elektro Jambura Journal of Electrical and Electronics Engineering MOTIVECTION : Journal of Mechanical, Electrical and Industrial Engineering Academia Open Indonesian Journal of Law and Economics Review Journal of Computer Networks, Architecture and High Performance Computing JiTEKH (Jurnal Ilmiah Teknologi Harapan) Journal of Electrical Engineering and Computer (JEECOM) Jurnal Pengabdian kepada Masyarakat JEEE-U (Journal of Electrical and Electronic Engineering-UMSIDA) Jurnal Janitra Informatika dan Sistem Informasi Indonesian Journal of Innovation Studies e-SPORT: Jurnal Pendidikan Jasmani, Kesehatan dan Rekreasi Jurnal Al Ulum: LPPM Universitas Al Washliyah Medan Jurnal Cakrawala Ilmiah Prosiding Seminar Nasional Teknik Elektro, Sistem Informasi, dan Teknik Informatika (SNESTIK) PELS (Procedia of Engineering and Life Science) Procedia of Social Sciences and Humanities SENAPAS Journal of Social Comunity Services BEGIBUNG: Jurnal Penelitian Multidisiplin Computing and Information System Journal SNTE
Arief Wisaksono
Program Studi Teknik Elektro, Universitas Muhammadiyah Sidoarjo, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : Medical Laboratory Technology Journal

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Detection of Salmonella typhi Using Multiplex and Monoplex PCR in Tifoid Fever Patients Andika Aliviameita; Meryn Linanda Agustin; Anggie Wedha Puspita; Miftahul Mushlih; Puspitasari Puspitasari; Yanik Purwanti; Arief Wisaksono
Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (700.217 KB) | DOI: 10.31964/mltj.v5i2.230

Abstract

Typhoid fever is a disease caused by Salmonella typhi infection. This disease is still a global problem. The purpose of this study was to develop a rapid detection method for Salmonella using molecular methods utilizing Inv A and Fim-C Genes. Two methods compared, which are Multiplex and monoplex PCR. The sample is in the form of the patient's blood, which is stored at 3 ml EDTA vacutainer tube consisting of 10 positive samples and five negative samples. The criteria for samples used previously have tested by widal/ immunoserology (titer more than 1/160). The results showed that multiplex PCR for detection of Salmonella using the Inv A gene and fim c gene not recommended because too many bands produced. The application of monoplex in the Inv A gene gives better results than the Fim C gene. In conclusion, the monoplex application on Inv A gene recommended than Fim C gene used to detect S. typhi in human blood samples.
Co-Authors A. Miftakhurrahmat Achmad Fatchur Rochman, Achmad Fatchur Achmad Karim Fadhillah Adam, Mochammad Rizki Ade Efiyanti Adhimanata, Yogi Adiansyah, Aldo Agus Hayatal Falah Ahmad Ahfas Ahmad Ahfas, Ahmad Ahmad Fudholi Ahmad Rifandi Akbar Ari Wibowo Akhmad Ahfas Alamsha, Mukhammad Ariya Amelia K, Paramitha Andika Aliviameita Anggie Wedha Puspita Anggraeni, Aprilia Putri Anis Farihah Anis Farihah, Anis Anjani, Kafita Luluk Arena, Ghinnata Alsyah Arief Rachman Yunanto Cylen Setyo Rini Darmaji, Muhammad Desyandri Desyandri Donny Wiraputra Dwi Hadidjaja Dwiki Maulana Rizaldi Efiyanti, Ade Egha Arya Fadly Hikmatus Sholichin Faris, Faisal Fauziyah, Mai Rizki Febrian, Dharma Ekita Putra Ferry Wahyu Perdana Hadiyatulloh, Moh Hindarto Ilham Ramadhana Chofananda Indah Sulistiyowati Indah Sulistyowati, Indah Ivan Danu Tirta Izza Anshory Jamaaluddin Lukman Hudi Masruchin Masruchin Masruchin Masruchin Mas’ud, Ibnu Maulidani, Muhammad Faza Maydia Anggraeni, Putri Meryn Linanda Agustin Miftahul Mushlih Mochammad Derian Ramadhan Mochammad Luthfan Hakim Mochammad Luthfan Hakim Mohamad Abid Sahuri Muhadzdzib Sayyidul Quthb Muhammad Aris Rafiansyah Muhammad Faris Muhammad Faris, Muhammad Muhammad Nasar Muhammad Shofiyuddin Muhammad Umar Mokhtar Novia Ariyanti Nurbaya, Syarifa Ramadani Phalah, Surya Mahendra Pian pian Pradana, Aldi Prasetyo, Yoga Eko Puspitasari Puspitasari Rachmad Aditya Pratama Rahmawati, Jurotul Islamiah Nur Rakhmad Fajar Ashari Ramadhan, Mochammad Derian Ribangun Bamban Jakaria Riski Yulianto Riski Yulianto Robbiatul Adawiyah Rossoleh, Andriyan Herqi Saickoni, Mohammad Sarwan Hamid Setyawan, Candra Dwi Setyo Rini, Cylen Shazana Dhiya Ayuni Sumarno Sumarno Sumarno Sumarno Syahrorini, Syamsudduha Syarifa Ramadani Nurbaya TATI NURHAYATI Tinara , Dinda Tia Tirta, Ivan Danu Wibowo, Akbar Ari wildan arief prasetyo Wiwik Sumarmi Yanik Purwanti Yoga Eko Prasetyo Yoga Eko Prasetyo