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All Journal Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal Kelautan Tropis Jurnal Sumberdaya Akuatik Indopasifik Omni-Akuatika Jurnal Ilmu Kelautan Kepulauan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine
Ismail, Firdaut
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology

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Journal : Journal of Fisheries

 1 
DNA Barcoding of Red Algae (Rhodophyta) in Ternate Island Sea, North Maluku, Indonesia Achmad, Muhammad Janib; Akbar, Nebuchadnezzar; Ismail, Firdaut; Samman, Ardan; Subhan, Beginer; E Paembonan, Rustam; Arafat, Dondy
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.44436

Abstract

Abstract Ternate Island is located on the North Maluku Sea. The North Maluku Sea region includes the Wallacea area and the world's coral triangle. One of the organisms commonly found in this sea is red algae. Study aimed to determine the red algae species by phylogenetic tree analysis based on the rbcL gene as a DNA marker. The preserved red algae tissue samples were extracted with the Geneaid GP100 DNA Extraction Kit Plant. The DNA sample was amplified and then visualized by 1% agarose gel electrophoresis. The amplicon products were sequenced and then aligned with the rbcL gene database that was available at the NCBI gene bank. The phylogenetic tree was constructed using the UPGMA method. The results showed that red algae were identified into four species: namely Gibsmithia hawaiiensis (98.65%), C_rbcL sample was identical to Amansieae sp. (91.50%), D_rbcL sample was identical to Peyssonnelia sp. (95.54%), and G_rbcL sample was similar to Portieria hornemanniI (96.15%). Based on phylogenetic tree analysis, Gibsmithia hawaiiensis from North Maluku is closely related to species from Raja Ampat, West Papua, Indonesia, followed by the Philippines species. Special findings were found to carry out phylogenetic reconstructions that can answer inter-species kinship. The general finding is that the markers used can be used for phylogenetic construction. Phylogenetic construction of Peyssonnelia sp. in North Maluku is related to species from South Africa. North Maluku's Portieria hornemannii is closely related to a species from Korea. Highlight Research This study provides initial information about the DNA barcoding of red algae. Important for management purposes and determining the status of red algae in the future. This scientific information also provides an understanding of the relationships among red algae species in the world's oceans. Global warming has changed the temperature of seawater and affected the population of marine organisms.
Biodiversity and DNA Barcoding of Seagrass in Equatorial Latitudes Regional Representation of Tropical Indonesia Would Improve Clarity, Structure, and Scientific Precision Subur, Riyadi; Akbar, Nebuchadnezzar; Abubakar, Salim; Ismail, Firdaut; Paembonan, Rustam E; Subhan, Beginer; Arafat, Dondy; Irfan, Muhammad
Jurnal Ilmiah Perikanan dan Kelautan 2026: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2026)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i2.83915

Abstract

Graphical Abstract Highlight Research 1. This research provides initial and current information regarding seagrass on small islands right on the equator. 2. directly depicts the diversity, density and cover of seagrass in a unique region, namely a small island crossed by the equator. 3. The environmental conditions of marine waters in seagrass areas on islands crossed by the equator have been described. 4. The latest information specifically describing seagrass and the ecology of equatorial marine environments. Essential for the development of future knowledge and technology. 5. DNA barcoding was capable of resolving field identification challenges and providing taxonomic clarification based on DNA sequence data.   Abstract The equator is a hypothetical line that separates the general region from the world.  Complete information on coastal ecosystems has not been provided for seagrass in the equatorial latitudes. Seagrass is dynamically distributed throughout the equatorial latitudes. The area that the equatorial latitudes also traverse is known as the indotropic region. Eastern Indonesia's Kayoa Island is situated directly on the equator.  A problem, namely how is the biodiversity of seagrass in the equatorial region. There are no published research on seagrass, particularly on biodiversity. The aim of this study is to reveal the diversity of seagrass in equatorial latitudes on the small islands. The methodology in this study was purposive sampling with a quadrant line transect was used to obtain the samples.  In situ identification of seagrass species was done.  Seagrass samples were taken from the leaves for DNA analysis purposes. Leaf samples were analyzed molecular in the laboratory. To determine the distribution of seagrass ecology and environmental were subjected to PCA analysis. The results found that six species of seagrass.  It has been discovered that seagrass may grow and develop in the tropical region under typical environmental conditions.  Despite the fact that six species were discovered, diversity was limited.  In this region, seagrass cover is typically high and supports a healthy species density.  In general concluded that diversity and density are low but cover is still quite good. DNA barcoding has revealed differences in morphological identification.This discovery represents preliminary data that can be investigated further in subsequent studies.    
Co-Authors Abdul Motalib Angkotasan Abdurrachman Baksir, Abdurrachman Abjan Ibrahim, Abjan Abubakar, Salim Achmad, M Djanib Achmad, M Janib Achmad, Muhammad Janib Angkotasan, Abdul M Anwar, Muhammad Fathur Ardan Samman Arif, Sanindo M Asep Sandra Budiman, Asep Sandra Baddu, S Baddu, Sartini Bahar Subur, Abd. Beginer Subhan Darmiyati Muksin Daud, Asmar Hi Dietriech Geoffrey Bengen Dondy Arafat E Paembonan, Rustam Hari, Restu Fajar I Wayan Nurjaya Ikbal Marus, Ikbal Irmalita Tahir, Irmalita Karman, Amirul Kotta, Raismin Muhammad Irfan Mutmainnah Mutmainnah N Natih, Nyoman Metta N, Furqan Najamadidin, Najamuddin Najamuddin Najamuddin, Najamuddin Nebuchadnezzar Akbar NEVIATY PUTRI ZAMANI Nurdiansah, Doni Nyoman M N Natih Paembonan, Rustam E Raden, Muhammad Sahlan R Rahimah, Insaniah Rahman Rahman Ramili, Yunita Rasidi, Rasidi Restu, Yunan Gilang Riyadi Subur, Riyadi Rustam Effendi Paembonan, Rustam Effendi Salnuddin Salnuddin, Salnuddin Salnuddin, Salnudin Samad, Julkar Simon I Patty Siolimbona, Abdul Ajiz Supyan Supyan Wahab, Iswandi Waluyo Waluyo Wibowo, Eko S Wibowo, Eko Setyobudi Yidoatimojo, Sudibyo Zulham Apandy Harahap Zulhan A Harahap, Zulhan A Zulhan Arifin Harahap