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Viabilitas Spermatozoa Semen Beku Babi Duroc Dalam Extender Beltsville Thawing Solution Menggunakan Krioprotektan Gliserol Dan Dimetillacetamida Nancy D. F. K. Foeh; Raden Iis Arifiantini; Tuty Laswardy Yusuf
JURNAL KAJIAN VETERINER Vol 4 No 1 (2016): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v4i1.1013

Abstract

This study investigated the effect of glycerol and dimethylacetamide (DMA) in beltsville thawing solution (BTS) extender. Semen samples were collected from three boars of the Duroc breed (n=5) and were evaluated microscopically and macroscopically. Semen that have been evaluated and meets the following criteria: has motility characteristics >70%, concentration >300 million/ml and abnormality 20%; was accepted as appropriate samples. Semen samples were divided into four tubes and were diluted with 5 ml of BTS. The diluted semen was left for two hours in the temperature range from 20 to 22°C before was centrifuged for 15 minutes (500G). The pellet of centrifuged semen was taken as much as 1 ml with its supernatant. The BTS glycerol (BTSG), BTS-DMA (BTSD), BTS-Glycerol-DMA (BTSG-D) were added into the pellet. The diluted semen was packed into straw of 0.5 ml and was equilibrated for 2 hours in 4 °C. The equilibrated semen was frost with the steam of nitrogen and stored in liquid nitrogen. The quality of frozen semen was assessed at least 24 hour after freezing process. The results indicate that the sperm motility after thawing in BTS extender BTSD is 52.14±0.4%, ,higher than the other two extenders (P<0.05). Thus, it is conclude that the DMA concentration in BTS extender maintains the quality of frozen semen from the Duroc breed better than other extenders.
Sari Buah Lontar Sebagai Pengencer Alami Dalam Mempertahankan Kualitas Spermatozoa Babi Nancy D. F. K. Foeh; Cynthia D. Gaina
JURNAL KAJIAN VETERINER Vol 5 No 1 (2017): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v5i1.1024

Abstract

The aim of this study was to investigate the effect of Siwalan Palm Juice as a natural extender for maintaining boar sperm quality. Semen ejaculates collected from three Duroc and Landrace boars were macroscopically (volume, colour, odor, pH and consistency) and microscopically (motility, concentration and abnormality) evaluated. Semen samples fulfilled all criteria for semen characteristic (motility > 70%, concentration 200 x 106 cells /ml and abnormality ≤ 20%). Antibiotics were added in this semen samples. This study used completely randomized design with 6 treated groups, each group repeated three times. K0 (control): fresh semen; K1: Siwalan juice + Landrace boar semen; K2: Coconut water + Landrace boar semen; K3: NaCL + Landrace boar semen; K4: Siwalan juice + Duroc boar semen; K5: Coconut water + Duroc boar semen; K6: NaCL + Duroc boar semen. This semen samples were stored under room temperature at 220C. The result shows that Duroc and Landrace boar spermatozoa can be stored in Siwalan Palm Juice extender for up to 24 hours. It can concluded that Siwalan Juice could be used as one of natural extender for maintaining boar sperm quality under room temperature at 220C.
EFEKTIVITAS BAKTERI ASAM LAKTAT YANG DIISOLASI DARI SUSU KUDA SUMBA TERHADAP KUALITAS SILASE JERAMI PADI Annytha Ina Rohi Detha; Frans Umbu Datta; Elisabet Beribe; Nancy D. F. K. Foeh; Nemay Ndaong
JURNAL KAJIAN VETERINER Vol 6 No 1 (2018): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v6i1.1053

Abstract

This study aims to evaluate the ability of lactic acid bacteria isolated from Sumba horse milk as a starter in improving the quality of rice straw silage. Determination of the quality of rice straw silage through organoleptic tests, measurement of percentage of silage damage and silage pH. there are 7 treatments based on silage compositions and lactic acid bacteria. The results showed the presence of golden color in all treatments as the original color, the texture was very soft to soft and not slimy, and the presence of a distinctive silage odor or close to the typical smell of silage in all treatments. The presentation of silage damage to rice straw in the positive control group showed the highest percentage of damage that was equal to 43.58%, while the percentage of damage in the treatment group ranged from 28.57% to 41.02%. The pH results showed the control group had a pH of 3.6 and the P1-P6 group had an average pH ranging from 5.7 to 6.5. The addition of lactic acid bacteria in the manufacture of silage in rice straw provides good quality silage results.
EFEKTIVITAS BAKTERI ASAM LAKTAT ASAL CAIRAN ISI RUMEN SAPI BALI TERHADAP BERBAGAI VARIABEL MUTU SILASE JAGUNG Frans Umbu Datta; Nadya Daramuli Kale; Annytha Ina Rohi Detha; Imanuel Benu; Nancy D. F. K. Foeh; Nemay A. Ndaong
JURNAL KAJIAN VETERINER PROSIDING SEMINAR NASIONAL KE-7
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v0i0.1587

Abstract

Silage is the result of fermentation from feed ingredients that have high water content (about 50% -80%) such as corn (Zeamays L), in a vacuum (anaerobic) by lactic acid bacteria. Making silage is one alternative to traditional biotechnology that can be done, especially utilizing the availability of local food sources. The purpose of this study is to determine the ability of lactic acid bacteria isolated from rumen fluid in Bali cattle as a starter in making corn forage silage, and evaluate the quality of corn forage silage provided by lactic acid bacteria isolated from rumen fluid in Bali cattle. The method used in this research is, making probiotics, making samples and testing the quality of silage. The results of this study are, lactic acid bacteria from the rumen contents of Balinese cattle rumen can be used as corn forage silage starter, this is indicated by the silage color in the range of yellowish green to brownish green which indicates that the silage is of good quality, silage aroma in the range of score 2 , 33-2.83 which produces a fresh sour aroma to near fresh-smelling acid, silage pH before being injected with Escherichia coli with an average of 4.51-4.81 which is within the normal range. Corn forage silage given lactic acid bacteria from the rumen contents of Bali cattle showed good quality, this is seen from the final silage results given by pathogenic bacteria Escherichia coli which showed silage color with an average of 1.83-2.58 indicating that the silage color included in the optimal range of brownish green, silage aroma in the range of 1.42-2.75 which gives a fresh sour aroma, silage pH in the range of 4.42-4.58 which indicates silage is in good range, dry silage content ranges from 32.4% -34.4% which is below the normal range, and the average percentage of damage is 0% - 3% which shows the difference in damage presentation between silage given by lactic acid bacteria and control treatment.
DESKRIPSI MORFOLOGIS NEMATODA SALURAN PENCERNAAN KAMBING KACANG (Capra hircus aegagrus) DI KOTA KUPANG-NUSA TENGGARA TIMUR Frans Umbu Datta; Theresia Tinenti; Annytha Ina Rohi Detha; Nancy D. F. K. Foeh; Nemay A. Ndaong
JURNAL KAJIAN VETERINER PROSIDING SEMINAR NASIONAL KE-7
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v0i0.1588

Abstract

Local Goats (Capra hircus aegagrus) is a type of small ruminants and is a home-grown in Indonesia which is very susceptible to attack by diseases caused by gastrointestinal parasites, especially in developing countries, causing losses to infected animals and economic losses to farmers. This study aims to know the morphology and total of eggs and adult worms, in the digestive tract in Kupang City- East Nusa Tenggara. This study uses work methods including total calculation and identification of worm eggs using the McMaster method, aiming to calculate the number of worm eggs and used to identify the morphology of worm eggs, total testing and morphology of adult worms in the digestive tract aims to determine the number of adult worms in the digestive tract, Semichenetic Acetic Camine staining aims to confirm and clarify the type from the results of identification of adult worms found in goat intestines after it is determined the dominant type of adult worms in the digestive tract of local goats. The results showed that there were three types of worm eggs found from 20 local goats, namely Trichuris sp as many as 5, Strongyloides sp as many as 63, and Strongyloid sp as many as 304 and there were three categories of infection, namely infection-free category in two goats (TTGT = 0), mild infections in three goats (TTGT = 300-400 or ranged from 1-499), and moderate infections in 15 goats (TTGT = 500-2850 or ranged from 500-> 5000). The results of the total study of adult worms in the digestive tract from four local goats found one type of digestive tract worms, namely Trichuris sp as many as 120 of the two goats (numbers 2 and 4). Trichuris sp as the most dominant type of worm in the digestive tract of local goats in Kupang City, East Nusa Tenggara.
UJI AKTIVITAS ANTIMIKROBA BAKTERI ASAM LAKTAT CAIRAN RUMEN TERHADAP PERTUMBUHAN Salmonella Enteritidis, Bacillus cereus, Escherichia coli DAN Staphylococcus aureus MENGGUNAKAN METODE DIFUSI SUMUR AGAR Frans Umbu Datta; Angela Novita Daki; Imanuel Benu; Annytha Ina Rohi Detha; Nancy D. F. K. Foeh; Nemay A. Ndaong
JURNAL KAJIAN VETERINER PROSIDING SEMINAR NASIONAL KE-7
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v0i0.1590

Abstract

Rumen liquid is a waste product of slaughterhouse that has the potential to be a pollutant, contains lactic acid bacteria which can be used as bio preservatives in food. The purpose of this study was to identify the antimicrobial activity of lactic acid bacteria (LAB) isolates from rumen fluid against Gram positive and Gram negative bacteria using well diffusion and disc diffusion methods and using lactic acid bacteria isolates (supernatant) and non-filtrate from rumen fluid. The main research materials used were LAB rumen fluid isolates, MRSA media (Mann Rogosa Sharpe Agar), MRSB media (Mann Rogosa Sharpe Broth), MHA media (Muller Hinton Agar), and pathogenic bacteria Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella Enteritidis. The results of the study showed that the LAB of rumen fluid carried out as an active LAB with Gram positive characteristics, round shape, negative catalase and non motile. Based on the results of testing the antimicrobial activity of lactic acid bacteria from rumen fluid isolates against pathogenic Gram positive (B. cereus and S. aureus) and Gram negative bacteria (Escherichia coli and Salmonella Enteritidis) using well and disc diffusion methods showed that Gram negative bacteria were more sensitive to antimicrobial of LAB compared to Gram positive bacteria. The diameter of the larger inhibition zone is produced using the disc method with the inhibition zone diameter range of 13.66-28.3 mm, while the well method ranges from 0-24.2 mm. The antimicrobial activity of LAB using non filtrate BAL produce inhibition zone diameter size range of 0-26.1 mm, while the filtrate BAL produce inhibition zone diameter range of 0-28.3 mm with the optimum time to produce antimicrobial activity 48 hours compared to 24 hours after incubation.
Hubungan Ukuran Testis Terhadap Motilitas Dan Viabilitas Spermatozoa Babi Landrace Dan Babi Duroc Yohanes Raldy Nadja; Cynthia Dewi Gaina; Nancy D. F. K. Foeh; Tarsisius Considus Tophianong
JURNAL KAJIAN VETERINER PROSIDING SEMINAR NASIONAL KE-7
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v0i0.1725

Abstract

Pertumbuhan populasi babi di Indonesia disetiap daerah dari tahun ke tahun mengalami peningkatan. Testis berfungsi menghasilkan spermatozoa dan hormon testosteron. Penelitian ini bertujuan untuk mengetahui pengaruh ukuran testis terhadap kualitas spermatozoa babi Landrace dan babi Duroc dengan umur berkisar 2-4 tahun. Penelitian yang digunakan adalah penelitian eksperimental meliputi pengukuran testis dan evaluasi semen secara makroskopis dan mikroskopis. Data mikroskopis yang diperoleh dianalisis secara deskriptif. Hasil penelitian rerata evaluasi semen babi Landrace secara mikroskopis menunjukkan motilitas 82.5±0.00 % dan viabilitas 88.7±0.00 %, sedangkan evaluasi makroskopis semen babi Duroc menunjukka motilitas 82.5±0.00 % dan viabilitas 88.6±0.00 %. Hasil rerata ukuran panjang testis babi Landrace bagian kiri 12.66 ± 3.0 cm, bagian kanan 14.00 ± 1.0 cm, lebar testis bagian kiri 7.66 ± 2.081 cm, bagian kanan 8.00±0.00 cm. Babi Duroc memiliki panjang testis kiri 16.66 ± 5.507 cm, panjang testis kanan 13.66 ± 3.21cm , lebar testis bagian kiri 8.3 ± 2.020 cm dan lebar testis bagian kanan 8.5 ± 1.5 cm. Dapat disimpulkan bahwa tidak terdapat perbedaan ukuran testis ternak babi Landrace dan Duroc nyata terhadap motilitas dan viabilitas spermatozoa (p>0.05) kedua jenis babi tersebut.
THE QUALITY OF BOAR FROZEN SEMEN DILUTED IN BTS® AND MII® WITH DIFFERENT CRYOPROTECTANT SUPPLEMENTED WITH SODIUM DODECYL SULPHATE Nancy Diana Frederika Katerina Foeh; Raden Iis Arifiantini; Tuty Laswardi Yusuf
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (387.223 KB) | DOI: 10.24815/jts.v%vi%i.5809

Abstract

This research was aimed to study the effect of administration of glycerol and dimetilacetamida (DMA) in BTS® and MIII®extender supplemented with sodium dodecyl sulphate (SDS) on boar frozen semen. A number of four boars were used in this study for semen collection (n=20). The collected semen was evaluated both macroscopically and microscopically. In this study, only the semen that demonstrated70% sperm motility, 200.106/mL sperm concentration, and20% sperm abnormalities were used and divided into eight tubes. A number of 4 tubes were diluted with 5 mL of BTS, and the rest with 5 mL MIII. The sampel was stored at 20-22° C for 2 hours, followed by centrifugation for 15 minutes (at 2000 rpm), and taken of pellet with 1 ml supernatant. The pellet that was resulted from centrifugation using BTS, then re-diluted with BTS-glycerol 5% (BTSG), BTS DMA 5% (BTSD), BTS-glycerol 5% and SDS (BTSG-S), BTS-DMA 5% and SDS (BTSD-S). Four other pellets that were centrifuged with MIII also re-diluted with MIII-glycerol 5% (MIIIG), MIII-DMA 5% (MIIID), MIII-glycerol 5% and SDS (MIIIG-S), MIII-DMA 5% and SDS (MIIID-S). Next, all of diluted semen were inserted into 0.5 mL straw and equilibrated for 2 hours (4° C), then frozen and stored in liquid nitrogen. The evaluation of frozen semen quality was conducted at 24 hours after frozen. The result of this study showed that post-thawing motility of spermatozoa in BTSD-S (40.17±0.2%) was found higher (P0.05) compared to seven other dilution processes. Therefore, it is concluded that the concentration of 5% DMA that supplemented with SDS in BTS dilution much better for maintaining boars frozen semen quality.
Service per Conception (S/C) dan Conception Rate (CR) Sapi Crossbreed (Persilangan Bos sondaicus dan Bos taurus) di Kecamatan Kupang Timur Tarsisius Considus Tophianong; Frans Umbu Datta; Tri Utami; Nancy Foeh; Cynthia Gaina; Yohanes Simarmata; Putri Pandarangga; Yeremia Y. Sitompul; Herlina Umbu Deta
JURNAL KAJIAN VETERINER Vol 11 No 1 (2023): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v11i1.10287

Abstract

Conception Rate (CR) and Service Conception (S/C) are two parameters for assessing the reproductive performance of cattle. This research was conducted to determine the value of S/C and CR in crossbreeding cattle in the working area of ​​the Public Health Center. The material used in this study was 72 crossbreed cattle aged 3-10 years. Semi-intensive rearing system with AI mating. The results the S/C in this study was 3.9 higher than the ideal standard of 1.6–2. The CR in this study was 58.6%. CR numbers are still relatively low from the ideal, namely 65 -75%.
Tinjauan: Implikasi Parasit Cacing pada Komposisi Tubuh Ternak Ruminansia Frans Umbu Datta; Nancy Foeh; Nemay Ndaong; Annytha Detha
JURNAL KAJIAN VETERINER Vol 11 No 1 (2023): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v11i1.10406

Abstract

The effects of gastrointestinal parasties on feed intake and growth is well-established. The emphasis here is on raising awareness that parasites do have effects on body compositon and meat quality atrributes. Body composition refers to the acceptable balance of lean to fat ratio when animals were parasitised or when they were free of parasites. Rejection of carcases due to parasitism is also covered. It was concluded that parasitic infections at subclinical level lead to reduced quantity and impaired quality of meat indirectly by in protein and energy intake as well as through altering and diverting of protein availability for both body maintenance in the forms of establishing resilience to infection as well as repairing of tissues damaged by parasites at sites of predilection. Ineficiency in meat production and alterations to body composition due to nematode parasitism is very much associated with alterations in nutrient absorption and utilisation.