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All Journal SRIWIJAYA JOURNAL OF MEDICINE Jurnal Kesehatan Abdimas Universal Jurnal Pengabdian Masyarakat: Humanity and Medicine
Rivani, Erizka
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Civil Engineering, Building, Construction & Architecture Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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Analisis Gen Blavim, Blandm dan Blaimp Carbapenemase dengan Alat Otomatis Vitek-2 dan Metode Polymerase Chain Reaction (PCR) pada Isolat Bakteri Enterobacteriaceae di RSUP Dr. Moh. Hoesin Palembang Sabrina, Tia; Rivani, Erizka; Patricia, Venny
Sriwijaya Journal of Medicine Vol. 1 No. 2 (2018): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.921 KB)

Abstract

Enterobacteriaceae is a normal flora in human intestinal tract which the most frequently caused the disease in human. Carbapenem is the last lines of antibiotic which used to treat severe infection that caused by gram-negative bacili bacteria, for example Enterobacteriaceae, but there is an increase prevalency of infection which caused by Carbapenem Resistant Enterobacteriaceae (CRE). The gene that encoding CRE is blaVIM, blaNDM, and blaIMP gene. The detection of these genes are very important to prevent spreading the nosocomial infection at hospital. The samples are from the speciment which has detected as isolate of Extended Spectrum Beta Lactamase (ESBL) Enterobacteriaceae bacteria and has occurred resistant to carbapenem group (meropenem and ertapenem) which detected by automatic equipment, VITEK 2 compact system. These samples will be processed by PCR with multiplex PCR technique to indentify blaVIM, blaNDM, and blaIMP gene. There are 43 samples consist of 33 samples (76,7%) ESBL bacteria isolate and 10 samples (23,3%) Carbapenemase bacteria isolate, 29 samples (67,4%) are Klebsiella pneumonia and 14 samples (32,6%) E. coli. These samples were from blood (5 samples/ 11,6%), sputum (16 samples/ 37,2%), peritoneal fluid (1 sample/ 2,3%), pus (4 samples/ 9,3%), urine (12 samples/ 28%), swab (3 samples/ 7%), tissue (1 sample/ 2,3%), and feces (1 sample/ 2,3%). From the PCR result, the gene was indentified from Enterobacteriaceae bacteria isolate was 28 samples (65,11%) from 43 samples. The indentified samples consist of 9 samples (32,1%) Carbapenemase bacteria isolate and 19 samples (67,9%) ESBL bacteria isolate. The identified of Enterobacteriaceae bacteria isolate consist of 15 samples blaNDM gene, 17 samples blaVIM gene, and 4 sample blaIMP gene.
Pemberdayaan kelompok ibu rumah tangga sebagai kader bijak antibiotik dalam upaya pengendalian resistensi antibiotik tingkat komunitas Farah Diba, Masayu; Amalia, Ella; Sabrina, Tia; Rivani, Erizka; Zanaria, Rima; Nawawi, Rizki Andini
Jurnal Pengabdian Masyarakat: Humanity and Medicine Vol 5 No 2 (2024): Jurnal Pengabdian Masyarakat: Humanity and Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/Hummed.V5I2.140

Abstract

Infectious diseases are a threatening health problem in developing countries, including Indonesia. Diseases caused by bacterial infections can be overcome relatively easily after the discovery of various antibiotics. Uncontrolled use of antibiotics will trigger antibiotic resistance. With the incidence of antibiotic resistance, the potential of antibiotics will be reduced in treating infections and diseases in humans, animals and plants. Community service activities aim to provide knowledge to the community about antibiotics, how to use antibiotics wisely, and introduction to the concept of antibiotic resistance. The activities carried out were in the form of empowerment in the form of counseling for the community and assistance in the form of training for groups of prospective antibiotic wise cadres in the Siring Agung Village area. The results of this activity were obtained to increase the insight and knowledge of the community and health cadres about Antibiotic Wisdom, especially the use of antibiotics correctly, how to get them, how to follow up on antibiotic drug waste if it remains. In addition, the community and cadres also have the skills to wash hands properly and correctly using running water and soap independently to prevent transmission of infectious diseases. Furthermore, it is hoped that continuous service activities with similar themes at different layers or levels of society can be carried out in order for the community to know the importance of the principle of using antibiotics wisely so that the incidence of antibiotic drug resistance can be suppressed to increase.
Pemeriksaan Status Gizi Santri Sebagai Upaya Skrining dan Pencegahan Penyakit Infeksi Nawawi, Rizki Andini; Amalia, Ella; Sabrina, Tia; Rivani, Erizka; Zanaria, Rima; Diba, Masayu Farah; Usman, Doni; Al-Fayed, Muhammad Sadad; Zefianto, Zefianto; Ansyori, Achmad Rifky; Mansur, M. Syafi'i
Abdimas Universal Vol. 7 No. 1 (2025): April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Balikpapan (LPPM UNIBA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36277/abdimasuniversal.v7i1.2396

Abstract

Nutrition is a contributing factor in the maintenance of optimal immune system functions. Disturbances in nutritional status, such as malnutrition, would impact various structures, mediators, and processes in the immune system in responding to pathogenic infections, hence increasing vulnerability to infections. Adolescents are an age group often considered “healthy”, but screening in this age group proves important in reassuring optimal growth and development, which in turn will also impact the next generation. Pesantren is an Indonesian educational institution serving adolescents with a boarding school system, so it is of high importance for adolescent santri to know their own nutritional status in preventing infectious diseases. This community service event was held at Pesantren Thawalib Sriwijaya to inform about the importance of nutritional status in prevention of infectious diseases. The activities comprised of classical counseling and anthropometric measurement of participating santri. There were 42 participating santri attending Madrasah Tsanawiyah (MTs) and Madrasah Aliyah (MA) in this event, and plotting results showed most santri had normal nutritional status. However, nutritional status monitoring and awareness to infectious disease still needs to be kept up, as there are various other factors that may influence the spread of various infectious diseases.
Pelatihan tenaga kesehatan dalam penegakan diagnosis penyakit menular seksual dengan pemeriksaan mikroskopis sederhana di fasilitas kesehatan primer Rivani, Erizka; Nurwany, Raissa; Oktharina, Eka Handayani
Jurnal Pengabdian Masyarakat: Humanity and Medicine Vol 6 No 1 (2025): Jurnal Pengabdian Masyarakat: Humanity and Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/Hummed.V6I1.162

Abstract

Sexually transmitted diseases (STDs) encompass a range of infectious diseases that can be attributed to parasites, bacteria, viruses, or fungi. Inaccurate diagnosis can lead to ineffective treatment and increase transmission rates. Furthermore, in an era of widespread antimicrobial resistance, it is imprudent to administer empirical antimicrobial treatment without revelation of potential etiology information which could leads to suboptimal clinical outcomes. Patients suffering from STDs frequently seek initial assistance at primary healthcare facilities, such as community health centres. Enhancing the understanding of health professionals regarding the current state of STDs, including proper diagnostic techniques, will contribute to the improvement of health services. A simple test that can be performed is a microscopic examination of specimens containing genital secretions. The accuracy of microbiological examination heavily relies on the quality of the specimen. Providing training to general practitioners, nurses, midwives, and laboratory personel on specimen collecting techniques and microscopic examination techniques is needed to enhance the precision of diagnosis. The training involved both presentations and hands-on experience in collecting specimens for microscopic examination, as well as techniques for simple microscopic examinations to confirm the etiological diagnosis of STDs. An increase in the trainees' understanding was evident after the training, as reflected in the rise in the average post-test score compared to the pre-test score. Health workers have also been able to independently practice specimen collection techniques and conduct microscopic examinations after attending the training. This training has improved health workers' proficiency in managing STDs and contributed to a higher diagnosis coverage rate.
Activity of Karamunting Leaf Extract (Rhodomyrtus tomentosa (Aiton) Hassk) as an Antibiofilm on Klebsiella pneumoniae Sabrina, Tia; Simanjuntak, Ricardo; Rivani, Erizka; Zanaria, Rima; Lusiana, Evi
Jurnal Kesehatan Vol 16 No 2 (2025): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v16i2.4982

Abstract

Klebsiella pneumoniae can cause serious infections. The use of antibiotics is often an option in treating infections, but bacteria can adapt by forming biofilms. Therefore, the search for alternative antibiofilms from natural sources is important. One potential plant is karamunting leaves (Rhodomyrtus tomentosa (aiton) hassk). This study aims to describe the potential of the ethanol extract of karamunting leaf as an antibiofilm against Klebsiella pneumoniae. This study is an in vitro descriptive laboratory test. A thick ethanol extract of karamunting leaves was then prepared in three concentration variations: 62.5µg/mL, 125µg/mL, and 250µg/mL. The positive control used was meropenem, and the negative control used was DMSO. The parameter measured for the inhibition and eradication test was biofilm thickness, which was read using a 590nm Microplate reader. In this study, the percentage of inhibition in the three concentrations was 90.65% at a concentration of 250 µg/ml, 86.69% at a concentration of 125µg/ml, and 87.22% at a concentration of 62.5µg/ml. During the eradication test, the percentages obtained were -10.67% at a concentration of 250µg/ml, 3.58% at a concentration of 125µg/ml, and 68.23% at a concentration of 62.5µg/ml. The ethanolic extract of karamunting leaves demonstrates good biofilm inhibitory activity against Klebsiella pneumonia at all tested concentrations. At a concentration of 62.5µg/ml, the extract exhibits good biofilm eradication activity, while at 125µg/ml and in the positive control, it shows poor activity. Interestingly, at a concentration of 250µg/ml, the extract acts as a biofilm promoter, enhancing biofilm formation.
Co-Authors Al-Fayed, Muhammad Sadad Ansyori, Achmad Rifky Diba, Masayu Farah Ella Amalia, Ella Evi Lusiana Farah Diba, Masayu Mansur, M. Syafi'i Nawawi, Rizki Andini Nurwany, Raissa Oktharina, Eka Handayani Patricia, Venny Ricardo Simanjuntak Sabrina, Tia Usman, Doni Zanaria, Rima Zefianto, Zefianto