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Analisis Gen Blavim, Blandm dan Blaimp Carbapenemase dengan Alat Otomatis Vitek-2 dan Metode Polymerase Chain Reaction (PCR) pada Isolat Bakteri Enterobacteriaceae di RSUP Dr. Moh. Hoesin Palembang Sabrina, Tia; Rivani, Erizka; Patricia, Venny
Sriwijaya Journal of Medicine Vol. 1 No. 2 (2018): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.921 KB)

Abstract

Enterobacteriaceae is a normal flora in human intestinal tract which the most frequently caused the disease in human. Carbapenem is the last lines of antibiotic which used to treat severe infection that caused by gram-negative bacili bacteria, for example Enterobacteriaceae, but there is an increase prevalency of infection which caused by Carbapenem Resistant Enterobacteriaceae (CRE). The gene that encoding CRE is blaVIM, blaNDM, and blaIMP gene. The detection of these genes are very important to prevent spreading the nosocomial infection at hospital. The samples are from the speciment which has detected as isolate of Extended Spectrum Beta Lactamase (ESBL) Enterobacteriaceae bacteria and has occurred resistant to carbapenem group (meropenem and ertapenem) which detected by automatic equipment, VITEK 2 compact system. These samples will be processed by PCR with multiplex PCR technique to indentify blaVIM, blaNDM, and blaIMP gene. There are 43 samples consist of 33 samples (76,7%) ESBL bacteria isolate and 10 samples (23,3%) Carbapenemase bacteria isolate, 29 samples (67,4%) are Klebsiella pneumonia and 14 samples (32,6%) E. coli. These samples were from blood (5 samples/ 11,6%), sputum (16 samples/ 37,2%), peritoneal fluid (1 sample/ 2,3%), pus (4 samples/ 9,3%), urine (12 samples/ 28%), swab (3 samples/ 7%), tissue (1 sample/ 2,3%), and feces (1 sample/ 2,3%). From the PCR result, the gene was indentified from Enterobacteriaceae bacteria isolate was 28 samples (65,11%) from 43 samples. The indentified samples consist of 9 samples (32,1%) Carbapenemase bacteria isolate and 19 samples (67,9%) ESBL bacteria isolate. The identified of Enterobacteriaceae bacteria isolate consist of 15 samples blaNDM gene, 17 samples blaVIM gene, and 4 sample blaIMP gene.
The Effectiveness of Karamunting Leaf’s Fraction (Rhodomyrtus tomentosa (Aiton) Hassk) as Antimicrobials in Carbapenemase Resistant Klebsiella pneumonia Sabrina, Tia; Kamaluddin, MT; Theodorus, Theodorus; Salni, Salni
Sriwijaya Journal of Medicine Vol. 4 No. 1 (2021): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/sjm.v4i1.225

Abstract

In the world, the incidence of nosocomial infections in hospitals had increased. There had been an increase in the incidence of infections caused by Enterobacteriaceae, one of them is Klebsiella pneumonia, which resistant to carbapenem in the worldwide. The consequences of increased rates of resistance to many drugs pose a high need for the discovery of new types of antibiotic drugs. Rhodomyrtus tomentosa (aiton) hassk has an antibacterial effect that has long been used by Indonesians as a traditional drug. This study aims to find out the effectiveness of karamunting leaf fraction as an antimicrobial in carbapenemase resistant K.pneumonia bacteria and to find out the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the active compound of karamunting leaves as antimicrobial in Carbapenemase resistant Klebsiella pneumonia (CRKP) bacterial isolates. This research was an experimental laboratory research in vitro that exploratory analytical. The results of the study obtained the value of MIC ethyl acetate fraction started at a concentration of 125 μg/ml and n-hexan fraction at a concentration of 4000 μg/ml. MBC value was 125 μg/ml for ethyl acetat fraction and n-hexan fraction of karamunting leaves was 8000 μg/ml. From the bacterial activity test obtained at a concentration 32000 μg/ml of n-hexan fraction, the average diameter of the inhibitory zone 7.80 ± 1.30 mm and ethyl acetate fraction at a concentration of 4000 μg/ml can inhibit the growth of CRKP bacteria with an average of inhibition zone diameter was 9.40 ± 1.67 mm. From the results of the analysis using the Independent T Test and mann whitney test obtained a probability value between all groups with positive control was <0.05. It can be concluded that the ethyl fraction of acetate and n-hexan leaves of karamunting leaves contains active compounds that can interfere with the integrity of CRKP bacterial cell walls or membranes so that the bacteria can die.
GAMBARAN INFEKSI Klebsiella pneumoniae PENGHASIL Extended-spectrum β-lactamase (ESBL) PADA PASIEN COVID-19 DI RSUP DR. MOHAMMAD HOESIN PERIODE JANUARI 2021-JUNI 2021 Ahmad, Qaedi; Sabrina, Tia; Diba, Masayu Farah; Amalia, Ella; Putra, Ramadhan Ananditia
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol. 10 No. 2 (2022): Special Issues: Jambi Medical And Health Sciences International Conference (JA
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

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Abstract

ABSTRACT Background: ESBL-producing Klebsiella pneumoniae infection can make medical costs increase, patients become longer in the hospital, and a worse prognosis in Covid-19 patients. Therefore, this study aims to find out the prevalence, characteristics of age, gender, specimen type, inpatient room, and pattern of antibiotic sensitivity of ESBL-producing Klebsiella pneumoniae in Covid-19 patients so as to help patients in the prevention and control of HAIs in Dr. Mohammad Hoesin Hospital. Method: This observational research used secondary data in the form of the status of Covid-19 patients identified as ESBL-producing Klebsiella pneumoniae at the Central Laboratory Installation of Mohammad Hoesin Hospital Palembang during the period January 2021-June 2021. The samples in this study were all medical records of Covid-19 patients who met the inclusion criteria. Data was processed and analyzed by univariate to determine the frequency distribution of each variable studied. Result: Prevalence of ESBL-producing Klebsiella pneumoniae in Covid-19 patients at Dr. Mohammad Hoesin Hospital is 48,6%. Based on Age, prevalence of ESBL-producing Klebsiella pneumoniae in Covid-19 patients are often found in elderly (41,2%). Based on gender, prevalence of ESBL-producing Klebsiella pneumoniae in Covid-19 patients are often found in female (64,7%). Based on specimen type, prevalence of ESBL-producing Klebsiella pneumoniae in Covid-19 patients are often found in sputum (47,1%). Based on the inpatient room, prevalence of ESBL-producing Klebsiella pneumoniae in Covid-19 patients are often found in non-intensive care unit (79,5%). Based on sensitivity patterns, isolates ESBL-producing Klebsiella pneumoniae are resistance to ampicilin/AMP (100%), cefazoline (100%), ceftriaxon (100%), aztreonam (91,2%), ceftazedim (85,3%), ampisilin/Sulbaktam (76,5%), ciprofloxacin (76,5%), trimethoprim/ sulfamethoxazole (58,8%) and gentamicin (55,9%). Conclusion: Prevalence of ESBL-producing Klebsiella pneumoniae in Covid-19 patients at the Central Laboratory Installation of Mohammad Hoesin Hospital Palembang during the period January 2020-June 2020 is 48,6%. Antibiotics that resistance to ESBL-producing Klebsiella pneumoniae in Covid-19 patients are ampicilin/AMP, cefazoline, ceftriaxon, aztreonam, ceftazdim, ampisilin/sulbaktam, ciprofloxacin, trimethoprim/sulfamethoxazole and gentamicin. Keywords: ESBL, Covid-19, HAIs ABSTRAK Pendahuluan: Infeksi Klebsiella pneumoniae penghasil ESBL bisa membuat biaya pengobatan menjadi bertambah, pasien menjadi lebih lama di rumah sakit, dan prognosis yang lebih buruk pada pasien Covid-19. Oleh karena itu, penelitian ini bertujuan untuk mengetahui prevalensi, karakteristik berdasarkan usia, jenis kelamin, jenis spesimen, asal ruang rawat inap, dan pola sensitivitas antibiotik Klebsiella pneumoniae penghasil ESBL pada pasien Covid-19 sehingga dapat membantu pasien dalam pencegahan dan pengendalian HAIs di RSUP Dr. Mohammad Hoesin. Metode: Penelitian deskriptif observasional ini menggunakan data sekunder berupa status pasien Covid-19 yang teridentifikasi Klebsiella pneumoniae penghasil ESBL di instalasi laboratorium sentral RSUP Dr. Mohammad Hoesin Palembang selama periode Januari 2021-Juni 2021. Sampel pada penelitian ini adalah seluruh data rekam medik pasien Covid-19 yang memenuhi kriteria inklusi. Data diolah dan dianalisis secara univariat untuk mengetahui distribusi frekuensi setiap variabel yang diteliti. Hasil: Prevalensi Klebsiella pneumoniae penghasil ESBL pada pasien Covid-19 di RSUP Dr. Mohammad Hoesin sebesar 48,6%. Berdasarkan usia, Klebsiella pneumoniae penghasil ESBL pada pasien Covid-19 banyak ditemukan pada lansia (41,2%). Berdasarkan jenis kelamin, Klebsiella pneumoniae penghasil ESBL pada pasien Covid-19 banyak ditemukan pada perempuan (64,7%). Berdasarkan jenis spesimen, Klebsiella pneumoniae penghasil ESBL pada pasien Covid-19 banyak ditemukan pada sputum (47,1%). Berdasarkan ruang rawat inap, Klebsiella pneumoniae penghasil ESBL pada pasien Covid-19 banyak berasal dari ruang rawat non intensif (79,5%). Berdasarkan pola sensitivitas, Klebsiella pneumoniae penghasil ESBL resisten terhadap ampisilin/ AMP (100%), sefazolin (100%), seftriakson (100%), aztreonam (91,2%), seftazdim (85,3%), ampisilin/ sulbaktam (76,5%), siprofloksasin (76,5%), trimetoprim/ sulfametoksazol (58,8%), dan gentamisin (55,9%) Kesimpulan: Prevalensi Klebsiella pneumoniae penghasil ESBL pada pasien Covid-19 di Instalasi Laboratorium Mikrobiologi RSUP Dr. Mohammad Hoesin Palembang selama periode Januari 2021-Juni 2021 sebesar 48,6%. Antibiotik yang resisten pada Klebsiella pneumoniae penghasil ESBL yaitu ampisilin/AMP, sefazolin, seftriakson, aztreonam, seftazdim, ampisilin/sulbaktam, siprofloksasin, trimetoprim/sulfametoksazol dan gentamisin. Kata kunci: ESBL, Covid-19, HAIs
Pemberdayaan kelompok ibu rumah tangga sebagai kader bijak antibiotik dalam upaya pengendalian resistensi antibiotik tingkat komunitas Farah Diba, Masayu; Amalia, Ella; Sabrina, Tia; Rivani, Erizka; Zanaria, Rima; Nawawi, Rizki Andini
Jurnal Pengabdian Masyarakat: Humanity and Medicine Vol 5 No 2 (2024): Jurnal Pengabdian Masyarakat: Humanity and Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/Hummed.V5I2.140

Abstract

Infectious diseases are a threatening health problem in developing countries, including Indonesia. Diseases caused by bacterial infections can be overcome relatively easily after the discovery of various antibiotics. Uncontrolled use of antibiotics will trigger antibiotic resistance. With the incidence of antibiotic resistance, the potential of antibiotics will be reduced in treating infections and diseases in humans, animals and plants. Community service activities aim to provide knowledge to the community about antibiotics, how to use antibiotics wisely, and introduction to the concept of antibiotic resistance. The activities carried out were in the form of empowerment in the form of counseling for the community and assistance in the form of training for groups of prospective antibiotic wise cadres in the Siring Agung Village area. The results of this activity were obtained to increase the insight and knowledge of the community and health cadres about Antibiotic Wisdom, especially the use of antibiotics correctly, how to get them, how to follow up on antibiotic drug waste if it remains. In addition, the community and cadres also have the skills to wash hands properly and correctly using running water and soap independently to prevent transmission of infectious diseases. Furthermore, it is hoped that continuous service activities with similar themes at different layers or levels of society can be carried out in order for the community to know the importance of the principle of using antibiotics wisely so that the incidence of antibiotic drug resistance can be suppressed to increase.
Pemeriksaan Status Gizi Santri Sebagai Upaya Skrining dan Pencegahan Penyakit Infeksi Nawawi, Rizki Andini; Amalia, Ella; Sabrina, Tia; Rivani, Erizka; Zanaria, Rima; Diba, Masayu Farah; Usman, Doni; Al-Fayed, Muhammad Sadad; Zefianto, Zefianto; Ansyori, Achmad Rifky; Mansur, M. Syafi'i
Abdimas Universal Vol. 7 No. 1 (2025): April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Balikpapan (LPPM UNIBA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36277/abdimasuniversal.v7i1.2396

Abstract

Nutrition is a contributing factor in the maintenance of optimal immune system functions. Disturbances in nutritional status, such as malnutrition, would impact various structures, mediators, and processes in the immune system in responding to pathogenic infections, hence increasing vulnerability to infections. Adolescents are an age group often considered “healthy”, but screening in this age group proves important in reassuring optimal growth and development, which in turn will also impact the next generation. Pesantren is an Indonesian educational institution serving adolescents with a boarding school system, so it is of high importance for adolescent santri to know their own nutritional status in preventing infectious diseases. This community service event was held at Pesantren Thawalib Sriwijaya to inform about the importance of nutritional status in prevention of infectious diseases. The activities comprised of classical counseling and anthropometric measurement of participating santri. There were 42 participating santri attending Madrasah Tsanawiyah (MTs) and Madrasah Aliyah (MA) in this event, and plotting results showed most santri had normal nutritional status. However, nutritional status monitoring and awareness to infectious disease still needs to be kept up, as there are various other factors that may influence the spread of various infectious diseases.
Liposome Photosensitizer with Enzyme from Black Soybean Tempeh: Formula Optimization and In Vitro Thrombolytic Activity Evaluation Azzahra, Farah Daffa; Mulyani, Laida Neti; Sabrina, Tia; Fithri, Najma Annuria
Science and Technology Indonesia Vol. 10 No. 3 (2025): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.3.903-915

Abstract

Stroke and myocardial infarction contributed significantly as the leading causes of global mortality rate, both commonly caused by thrombosis. Black soybean tempeh (BSBT), a traditional Indonesian food fermented with Rhizopus oligosporus fungus is rich in proteolytic enzymes, with potential to be utilized for thrombosis related ailments. Herein, we report the first findings of BSBT enzymatic activity and its subsequent formulation into liposomal system as a thrombolytic. Additionally, we incorporated photosensitizer dyes into the liposomes, phycocyanin and fluoresecein, creating a photothermally active therapeutic delivery system. Liposomes containing BSBTwere formulated using soy lecithin and tween 80, whichwere then subjected to evaluations including size, PDI, zeta potential, morphological, and stability studies. Furthermore, we observed their photothermal efficiency and thrombolytic activity using whole blood clot in vitro model. BSBT crude and purified extract produced satisfactory enzymatic activity, stable at neutral pH (∼7) and maintained stable activity at temperatures of ∼60◦C. Liposome formulation was spherical with a particle size of 607.8 nm; PDI of 0.339; and zeta potential of -24.2 mV. BSBT crude extract and purified enzyme at a concentration of 100% gave 51.28 and 56.05% thrombolytic activity. Based on the test results obtained, the optimum formula of photosensitizer liposomes produced had high encapsulation efficiency, with photothermal efficiency of 57.66 and 44.23% for Lip-Flu and Lip-Phy respectively. The formulations with laser exposure generated good thrombolytic activity (∼55-56%) comparable with nattokinase. Based on these findings, liposomal delivery of BSBT enzymes can maintain proteolytic activity, providing the first insights for thrombolytic purposes of BSBT enzymes.
Activity of Karamunting Leaf Extract (Rhodomyrtus tomentosa (Aiton) Hassk) as an Antibiofilm on Klebsiella pneumoniae Sabrina, Tia; Simanjuntak, Ricardo; Rivani, Erizka; Zanaria, Rima; Lusiana, Evi
Jurnal Kesehatan Vol 16 No 2 (2025): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v16i2.4982

Abstract

Klebsiella pneumoniae can cause serious infections. The use of antibiotics is often an option in treating infections, but bacteria can adapt by forming biofilms. Therefore, the search for alternative antibiofilms from natural sources is important. One potential plant is karamunting leaves (Rhodomyrtus tomentosa (aiton) hassk). This study aims to describe the potential of the ethanol extract of karamunting leaf as an antibiofilm against Klebsiella pneumoniae. This study is an in vitro descriptive laboratory test. A thick ethanol extract of karamunting leaves was then prepared in three concentration variations: 62.5µg/mL, 125µg/mL, and 250µg/mL. The positive control used was meropenem, and the negative control used was DMSO. The parameter measured for the inhibition and eradication test was biofilm thickness, which was read using a 590nm Microplate reader. In this study, the percentage of inhibition in the three concentrations was 90.65% at a concentration of 250 µg/ml, 86.69% at a concentration of 125µg/ml, and 87.22% at a concentration of 62.5µg/ml. During the eradication test, the percentages obtained were -10.67% at a concentration of 250µg/ml, 3.58% at a concentration of 125µg/ml, and 68.23% at a concentration of 62.5µg/ml. The ethanolic extract of karamunting leaves demonstrates good biofilm inhibitory activity against Klebsiella pneumonia at all tested concentrations. At a concentration of 62.5µg/ml, the extract exhibits good biofilm eradication activity, while at 125µg/ml and in the positive control, it shows poor activity. Interestingly, at a concentration of 250µg/ml, the extract acts as a biofilm promoter, enhancing biofilm formation.
UPAYA PENCEGAHAN CACINGAN MELALUI EDUKASI DAN PRAKTIK KEBERSIHAN TANGAN DAN KUKU Handayani, Dwi; Dalilah, Dalilah; Sabrina, Tia; Prasasty, Gita Dwi; Susilawati, Susilawati; Naue, Dian Adhe Bianggo; Rahmadona, Risa; Fadilatunnisa, Nur
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 5 No. 1 (2024): Volume 5 No 1 Tahun 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v5i1.19541

Abstract

Infeksi cacingan masih menjadi masalah kesehatan di masyarakat namun seringkali diabaikan. Anak-anak usia sekolah dasar merupakan kelompok yang rentan mengalami infeksi ini. Salah satu faktor risiko terjadinya infeksi cacingan adalah kebersihan tangan dan kuku yang buruk akibat rendahnya pengetahuan dan kesadaran dalam mencuci tangan dan memelihara kebersihan kuku. Kegiatan ini bertujuan untuk memberikan edukasi kesehatan tentang cacingan dan praktik mencuci tangan enam langkah menurut WHO. Sasaran kegiatan ini adalah santri dan orangtua/wali santri Rumah Tahfidz Yatim Dhuafa Persaudaraan Muslimah Ilir Timur 2 (RTYD Salimah IT 2) yang berlokasi di Lorong Wiraguna, Kelurahan Kuto Batu, Kecamatan Ilir Timur 2, Kota Palembang. Edukasi dilakukan melalui beberapa metode yaitu pengisian kuesioner, pemeriksaan tinja, penyuluhan, dan praktik/peragaan cara mencuci tangan dan memotong kuku. Sebanyak 72 orang santri hadir dalam kegiatan dan mengisi kuesioner berisi pertanyaan mengenai cacingan, kebiasaan mencuci tangan, dan kebersihan kuku melalui observasi. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan dan psikomotor setelah diberikan edukasi terkait cacingan dan mencuci tangan. Pada pemeriksaan tinja ditemukan satu sampel yang positif cacing Ascaris lumbricoides. Peningkatan pemahaman mengenai bahaya cacingan diharapkan dapat menumbuhkan kesadaran dan kemandirian dalam pencegahan cacingan di keluarga dan masyarakat.
PENINGKATAN LITERASI KESEHATAN TENTANG PENYAKIT INFEKSI EMERGING DAN REEMERGING MELALUI SEMINAR DARING NASIONAL BAGI MAHASISWA DAN TENAGA KESEHATAN Rivani, Erizka; Amalia, Ella; Sabrina, Tia; Diba, Masayu Farah; Nawawi, Rizki Andini; Usman, Doni
JPM (Jurnal Pengabdian Masyarakat) Ruwa Jurai Vol. 9 No. 2 (2024): JURNAL PENGABDIAN MASYARAKAT RUWA JURAI
Publisher : FK Unila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpmrj.v9i2.3403

Abstract

Penyakit infeksi emerging dan reemerging merupakan tantangan yang semakin signifikan dalam dunia kesehatan dalam beberapa tahun terakhir, terutama diakibatkan oleh makin berkembangnya globalisasi, urbanisasi, dan juga perkembangan teknologi di dunia. Berbagai penyakit infeksi baru bermunculan, dan penyakit infeksi yang telah dikenal sejak dahulu mengalami perubahan dari segi manifestasi klinik maupun tantangan dalam deteksi dan tata kelola. Hal ini membuat manajemen dari penyakit infeksi emerging dan reemerging menjadi unik dari segi kebaruan dan pengenalan penyakit. Pengabdian masyarakat dalam bentuk seminar bagi tenaga kesehatan diharapkan dapat meningkatkan kewaspadaan akan penyakit, termasuk bagi mahasiswa yang kelak akan menjadi bagian dari praktisi kesehatan. Seminar diadakan secara daring untuk memperluas cakupan acara sehingga dapat diikuti oleh peserta dengan berbagai latar belakang profesi kesehatan dari seluruh Indonesia. Materi yang disampaikan mencakup tantangan klinis dan manajemen penyakit serta peran laboratorium mikrobiologi klinik dalam deteksi dan pencegahan penyakit infeksi emerging dan reemerging. Terdapat peningkatan persentase jawaban benar dan nilai rerata dari post-test yang dikerjakan oleh peserta pasca paparan materi dibandingkan dengan pre-test. Adanya diskusi interaktif setelah paparan juga menunjukkan ketertarikan peserta akan materi yang disampaikan yang dinilai relevan untuk praktik klinis sehari-hari. Kegiatan pengabdian masyarakat ini memberikan dampak positif berupa peningkatan pemahaman dan motivasi peserta untuk menerapkan ilmu yang didapatkan dalam manajemen, deteksi, dan pencegahan penyakit infeksi emerging dan reemerging. Seminar daring diharapkan dan menjadi model edukasi berkelanjutan untuk meningkatkan kesiapsiagaan dalam menghadai perkembangan ilmu kesehatan secara global.
Pendampingan santri dalam pemanfaatan tanaman obat untuk mencegah anemia Lusiana, Evi; Saleh, Muhammad Irsan; Tamzil, Nia Savitri; Oktariana, Desi; Sabrina, Tia; Turridho, Aisya; Qonitah, Zahrah Aliyah
Jurnal Pengabdian Masyarakat: Humanity and Medicine Vol 6 No 3 (2025): Jurnal Pengabdian Masyarakat: Humanity and Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/Hummed.V6l3.178

Abstract

Anemia is a condition characterized by a deficiency of hemoglobin in the blood, which can impair productivity and health, particularly among vulnerable populations such as Islamic boarding school students (santri). Education and the utilization of herbal plants have potential as effective measures for anemia prevention. However, these approaches are underutilized in this population. This study aimed to enhance the knowledge and skills of santri regarding anemia prevention, the use of herbal plants, and health screening at the Modern Thawalib Sriwijaya Islamic Boarding School in Palembang, involving 40 participants (67.5% male, 32.5% female). Activities included educational sessions, health screenings, and guidance on herbal plant cultivation. Evaluations were conducted using pretest and posttest assessments. Results demonstrated significant improvements in knowledge (from 30% to 95%), attitudes (from 15% to 65%), and practices (from 47.5% to 82.5%). Hemoglobin screening revealed moderate anemia in 42.5% of participants, mild and severe anemia in 10% each, and no anemia in 37.5% of participants. Educational interventions effectively improved the understanding, attitudes, and behaviors of santri concerning anemia and the preventive potential of herbal plants.