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Uji Aktivitas Antibakteri Ekstrak Fermentasi Bawang putih tunggal (Allium sativum L.) Terhadap Bakteri Staphylococcus aureus dan Methicillin-resistant Staphylococcus aureus (MRSA) Sapitri, Alfi; Marbun, Eva Diansari; Arisetya, Dian
Herbal Medicine Journal Vol 8 No 1 (2025): Herbal Medicine Journal
Publisher : Program Studi S1 Farmasi, STIKES Senior, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58996/hmj.v8i1.152

Abstract

The pathogenicity of S. aureus bacteria is often associated with purulent wound infections in both humans and animals, which is the main cause of pyemia cases. Methicillin-Resistant Staphylococcus aureus (MRSA) is a Staphylococcus aureus bacteria that is resistant or immune to methicillin-type antibiotics and its derivatives. This study aims to see the activity of extracts from single garlic fermentation (Allium sativum L) which have antibacterial effects against Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria. This study was conducted at the Pharmacy Laboratory of Sari Mutiara Indonesia University in June-August 2024. This research method was carried out experimentally, the extraction was maceration and the media was tested using MHA to see the bacterial inhibition power. Based on the results of phytochemical screening examinations of single garlic fermentation extracts, it showed the presence of chemical compound groups in the form of Alkaloids, Flavonoids, Tannins, and Saponins. Where the concentration of single garlic fermentation extract is 5%; 10%; 15%. The results of the inhibition test against Staphylococcus aureus bacteria were 9.74 mm; 11.48 mm; 12.19 mm, positive control (chloramphenicol) 12.35 mm. The results of the inhibition test against Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria were 8.49 mm; 10.58 mm, 11.33 mm, positive control (chloramphenicol) 11.42 mm. Ethanol extract from single garlic fermentation has weak antibacterial activity against Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria.
Formulasi dan Evaluasi Sediaan Obat Kumur Dari Ekstrak Fermentasi Bawang Lanang (Allium sativum L.) Marbun, Eva Diansari; Sapitri, Alfi; Arisetya, Dian; Thaib, Cut Masyithah; Haliza, Aipak; Purba, Elizabet Kristina; Helmi, Helmi
Herbal Medicine Journal Vol 8 No 1 (2025): Herbal Medicine Journal
Publisher : Program Studi S1 Farmasi, STIKES Senior, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58996/hmj.v8i1.153

Abstract

Mouthwash is a dilute concentration of antibacterial solution used to fight oral microbes, fight oral infections, cleansers, to eliminate bad breath, fresh and antiseptic. Mouthwash plays an important role in an individual's oral hygiene, mouthwash helps to relieve symptoms of gingivitis, inflamed gums and can also be relied on to destroy pathogenic bacteria. (Banu & Gayathri, 2016). The purpose of this study was to see if the extract from fermented Bawang Lanang (Allium sativum L) can be formulated into a mouthwash. This research method is an experimental laboratory. By including preparation of materials, sample identification, Making fermented Bawang Lanang, making extracts, extract yields, phytochemical screening, then formulated into a mouthwash. After that, an evaluation of the physical preparation of the mouthwash was carried out (organoleptic test, pH test, viscosity test and stability test). The results of the evaluation of the mouthwash preparation starting from the organoleptic test, pH test did not exceed pH 7, viscosity test of the series was stable for 3 months. Conclusion: Mouthwash preparations containing fermented ethanol extract of male shallots can be used as mouthwash, with good stability.
Buah Pisang barangan (Musa acuminata linn) Sebagai Media Alternatif Perkembangbiakan Bakteri Lubis, Retnita Ernayani; Sapitri, Alfi; Arisetya, Dian; Marbun, Eva Diansari
Herbal Medicine Journal Vol 8 No 1 (2025): Herbal Medicine Journal
Publisher : Program Studi S1 Farmasi, STIKES Senior, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58996/hmj.v8i1.155

Abstract

Barangan banana (Musa acuminata linn) has an energy content of 89 calories, 22.84 grams of carbohydrates, 1.09 grams of protein and 0.33 grams of fat. The nutritional content and bioactive substances of barangan bananas have phenolics, anthocyanins and phenols. The purpose of this study was to determine whether barangan bananas can potentially be an alternative medium for bacterial growth. The growth method used in this study was the scratch method, with the calculation of the number of colonies using the TPC (Total Plate Count) method. The results of the study on the scratch method at various concentrations were able to grow optimally on barangan banana-based media at a concentration of 36%. The shorter exponential phase (log) period in the 36% concentration media with a higher cell density than in the 12% concentration media. Conclusion: Barangan banana (Musa acuminata linn) can potentially be an alternative medium for bacterial growth.
Edukasi Pemanfaatan Pisang Kepok sebagai Media Tumbuh Bakteri dan Aplikasi Fisika dalam Proses Pengolahan Tepung Pisang di SMA Negeri 7 Medan Lubis, Retnita Ernayani; Sapitri, Alfi; Diansari Marbun, Eva; Arisetya, Dian
Jurnal Pengabdian Masyarakat Nusantara (JPMN) Vol. 5 No. 1 (2025): Februari - Juli 2025
Publisher : Lembaga Otonom Lembaga Informasi dan Riset Indonesia (KITA INFO dan RISET)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35870/jpmn.v5i1.4746

Abstract

This community service activity aims to improve students' scientific literacy through education on the use of kepok bananas as an alternative medium for bacterial growth and the application of physics concepts in the process of processing banana flour. The program was implemented at SMA Negeri 7 Medan, which has limited science laboratory facilities, so that learning is still predominantly theoretical. The activity was designed through four main stages: initial survey, socialization, training, and mentoring. Students were guided to recognize the starch content in bananas as an energy source for microorganisms and to understand the principle of heat transfer in drying bananas. Implementation was carried out through direct practice of making banana flour and bacterial culture media based on local materials. Evaluation of the results showed a significant increase in student understanding, with more than 80% of students successfully making effective growth media marked by the formation of even bacterial colonies. In addition, 92% of students stated that this activity increased their understanding of science in an applicable and enjoyable way. This activity also opens up opportunities for cross-disciplinary integration (biology and physics) and the potential for entrepreneurship based on banana processing. The entire program proves that science learning based on local materials and direct practice can be an effective strategy to improve students' scientific skills and learning independence in high schools.
Aktivitas Antibakteri Ekstrak Etanol Bulbil Porang (Amophophallus muelleri Blume.) Terhadap Bakteri Staphylococcus aureus dan Streptococcus mutans Sapitri, Alfi; Marbun, Eva Diansari; Arisetya, Dian; Lubis, Retnita Ernayani
Herbal Medicine Journal Vol 8 No 2 (2025): Herbal Medicine Journal
Publisher : Program Studi S1 Farmasi, STIKES Senior, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58996/hmj.v8i2.161

Abstract

The porang is a fruit produced by the porang plant which is brownish black in color, oval in shape like a stone and the size is approximately the size of small marbles to large marbles, growing between the stems of the porang plant. This study aimed to determine the activity of the ethanol extract of the porang frog (Amorphophallus muelleri Blume) as an antibacterial against Staphylococcus aureus and Streptococcus mutans bacteria and to determine the chemical compounds contained in the porang frog (Amorphophallus muelleri Blume). Porang frog simplicia powder was extracted in 96% ethanol by maceration. Simplicia powder is screened to determine its phytochemical content. The antibacterial activity test was carried out using the Kirby Bauer method using paper discs against Staphylococcus aureus and Streptococcus mutans bacteria to determine the zone of inhibition. The simplicia results showed that the simplicia frog porang had 6.08% water content, 29.11% water soluble extract, 9.70% ethanol soluble extract, 93.51% total ash content, insoluble ash content in ethanol. acid 91.96%. Simplicia said porang contains alkaloids, flavonoids, and tannins.The ethanol extract of the porang frog (Amorphophallus muelleri Blume) has antibacterial activity against Staphylococcus aureus and Streptococcus mutans bacteria.
Aktivitas Antibakteri Ekstrak Etanol Daun Jengkol (Archidendron pauciflorum Benth.) Terhadap Bakteri Staphylococcus epidermidis dan Pseudomonas aeruginosa Arisetya, Dian; Sapitri, Alfi; Asfianti, Vivi; Lubis, Retnita Ernayani
Herbal Medicine Journal Vol 8 No 2 (2025): Herbal Medicine Journal
Publisher : Program Studi S1 Farmasi, STIKES Senior, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58996/hmj.v8i2.162

Abstract

Archidendron pociflorum benth is a nutritious plant that is used as a traditional medicine that is often used by society. Secondary metabolic compounds contained in archidendron pociflorum leaves are alkaloids, flavonoids, tannins and saponin. The purpose of this research is to know the antibacterial activity of the extract of archidendron pociflorum leaf ethanol from the Staphylococcus epidermidis and Pseudomonas areuginosa bacteria. Research conducted experimentally including the collection and processing of archidendron pociflorum leaves. The powder simplisia leaves archidendron pociflorum then be done characteristic and macerated using ethanol 96%. Further extracts of archidendron pociflorum leaf ethanol in the phytochemical screening and carried out test antibacterial activity against Staphylococcus epidermidis and pseudomonas areuginosa bacteria use the diffusion method to make it with disc paper. Results characteristic simplisia of archidendron pociflorum leaves obtained water content of 8.1%, water soluble sari content of 9.49%, soluble pollen content of 7.61%, total ash content 3.80%, ash content of insoluble acid 14.67%. Scining extracts of archidendron pociflorum leaves and simplisia indicate the presence of alkaloid, tannins, saponin, flavonoids. The test results of antibacterial activity indicate that the leaf ethanol extract of the staphylococcus epidermidis bacteria with a concentration of 80%, 60%, 40%, 20% respectively had an obstacle zone of 13.6 mm, 12.3 mm, 11, 4mm and 9.7 mm. While in bacteria pseudomonas areuginosa with a concentration of 80%, 60%, 40%, 20% respectively has a inhibitory zone of 14.3 mm, 13.1 mm, 11.2 mm and 10.3 mm. Archidendron pociflorum leaf ethanol extract has been effective against antibacterial activity with a concentration of 80% against bacteria staphylococcus epidermidis and pseudomonas aureginosa.
Integrasi Budaya dan Keanekaragaman Hayati Kawasan Danau Toba dalam Pembelajaran Biologi untuk Penguatan Profil Pelajar Pancasila Lubis, Retnita Ernayani; Sapitri, Alfi
Toga Jurnal Keguruan dan Ilmu Pendidikan Vol. 2 No. 1 (2025): April 2025
Publisher : Ilmu Bersama Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56211/toga.v2i1.1054

Abstract

Penelitian ini bertujuan untuk mengintegrasikan kekayaan budaya dan keanekaragaman hayati kawasan Danau Toba ke dalam pembelajaran biologi, guna memperkuat Profil Pelajar Pancasila. Melalui pendekatan kualitatif, data dikumpulkan dari observasi lapangan, wawancara dengan guru dan siswa, serta studi literatur. Hasil penelitian menunjukkan bahwa integrasi ini meningkatkan pemahaman siswa terhadap materi biologi dan nilai-nilai budaya lokal, serta memperkuat karakter sesuai dengan dimensi Profil Pelajar Pancasila
Pembelajaran Kultur Jaringan Tanaman Obat Berbasis Project Base Learning (PjBL) Terintegrasi Lesson Study: Plant Tissue Culture Learning Based on Project Based Learning (PjBL) Integrated Lesson Study Sapitri, Alfi; Tambunan, Muhammad Iqbal H.; Lubis, Retnita Ernayani; Arisetya, Dian
BIODIK Vol. 10 No. 4 (2024): Desember 2024
Publisher : Program Studi Pendidikan Biologi FKIP Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biodik.v10i4.38756

Abstract

The learning process that occurs still focuses on the lecturer as the main source of knowledge. So, to develop superior graduate competencies, learning development is needed by implementing project based learning (PjBL) integrated lesson study in learning medicinal plant tissue culture. In project learning (PjBL) students are required to be able to master theoretical concepts in the field of knowledge in depth, procedurally, analyze data, be able to solve problems, make decisions based on information and data analysis, and provide and choose alternative solutions. The aim of this research is to increase the learning effectiveness of pharmacy students. The research method used is Development or Research and Development (R&D). Lesson Study activities are carried out in 3 stages, namely planning, implementing (do) accompanied by project-based learning, and reflection (see). The research subjects were 50 pharmacy students from Sari Mutiara University, Indonesia. The research results show that: the application of PjBL to medicinal plant tissue culture which is integrated in lesson study can improve student learning outcomes as seen from the pre-test results of 37.4% and post-test results of 83.9%. The project carried out by students on medicinal plant tissue culture in all 10 groups resulted in the production of 100% sterile media, the results of explant initiation were 94% sterile and the explants grew 99.3%.
KEGIATAN PENYULUHAN DAYA SIMPAN SIRUP APEL HIJAU (Pyrus malus) DENGAN VARIASI GULA Nurbaya, Siti; Sitorus, Elly; Sapitri, Alfi
Jurnal Abdimas Mutiara Vol. 1 No. 1 (2020): JURNAL ABDIMAS MUTIARA
Publisher : Universitas Sari Mutiara Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sirup merupakan minuman yang biasa disajikan baik untuk tamu maupun keluarga. Berbagai merek sirup dipasaran dengan adanya bahan pengawet yang mungkin dapat mengurangi kandungan antioksidan pada sirup. Sehingga perlu adanya antioksidan alami yaitu sirup yang berasal dari apel hijau. Sirup apel hijau (Pyrus malus) memiliki umur simpan yang dipengaruhi dengan banyaknya penambahan gula dikarenakan gula yang diberikan pada suatu produk dengan kadar tinggi, dapat meningkatkan tekanan osmosis yang tinggi sehingga dapat mencegah pertumbuhan mikroba sehingga bahan akan menjadi awet. Tujuan dari kegiatan pengabdian masyarakat ini dilakukan untuk memberikan informasi dan edukasi daya simpan sirup apel hijau (Pyrus malus) dengan variasi gula. Hasil kegiatan pelaksanaan program pengabdian masyarakat dalam bentuk penyuluhan kepada masyarakat daya simpan sirup apel hijau (Pyrus malus) dengan variasi gula, memiliki daya simpan yang baik, mencegah terbentuknya mikroba, sehingga kandungan antioksidannya tetap terjaga. Sirup apel bermanfaat bagi kesehatan antara lain memperlancar saluran pencernaan, antioksidan, membantu dalam melawan sel-sel kanker, serta menjaga kesehatan gigi dan mata. Kesimpulan bahwa penyuluhan terlaksana sesuai pelaksanaan dan rencana, mendapat sambutan yang baik dari pemerintah setempat Kelurahan Gaharu, Kecamatan Medan Timur, Kota Medan, masyarakat mendapatkan informasi serta pengetahuan dan menerapkan informasi tersebut bagi keluarga dan masyarakat sekitar.
Aktivitas Antibakteri Ekstrak Etanol Umbi Porang (Amorphophallus muelleri Blume) terhadap Propionibacterium acnes dan Staphylococcus epidermidis Sapitri, Alfi; Marbun, Eva Diansari; Lubis, Retnita Ernayani; Arisetya, Dian
Herbal Medicine Journal Vol 9 No 1 (2026): Herbal Medicine Journal
Publisher : Program Studi S1 Farmasi, STIKES Senior, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58996/hmj.v9i1.208

Abstract

Porang tubers, scientifically known as Amorphophallus muelleri Blume, have shown that they possess compounds like alkaloids, flavonoids, saponins, and tannins. Because of this, researchers are looking closely at porang tubers to see how they can fight bacterial growth. Among the bacteria being investigated are Propionibacterium acnes and Staphylococcus epidermidis, as scientists search for new treatments for skin infections. Aim of reserach to assess how effective the antibacterial properties are and to analyze how different levels of extract concentration affect the growth of Propionibacterium acnes and Staphylococcus epidermidis. The method used for testing involved a paper disc diffusion technique with extract concentrations of 5, 15, 25, 35, and 45%, as well as Clindamycin and 10% DMSO. The findings revealed that the ethanol extract from porang tubers displayed antibacterial effects at concentrations ranging from 5% to 45%, resulting in inhibition zones of 9. 65 mm to 13. 56 mm against Propionibacterium acnes. Additionally, it showed inhibition zones of 9. 95 mm to 16. 07 mm against Staphylococcus epidermidis in the same concentration range. According to the One Way Anova test, the results showed a significant difference indicating that varying concentrations of the ethanol extract from porang tubers greatly influenced the growth of both Propionibacterium acnes and Staphylococcus epidermidis. The ethanol extract of porang tubers begins to show antibacterial effects at a minimum concentration of 5% against both Propionibacterium acnes and Staphylococcus epidermidis, with observable differences in the average inhibition zones for each concentration tested.