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AA Istri Mas Padmiswari
Institut Teknologi Dan Kesehatan Bali
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Peningkatan Pengetahuan Dengan Pemberian Penyuluhan Tentang Hipertensi Pada Lansia Di Desa Melinggih Kecamatan Payangan Gianyar Dharmapatni, Ni Wayan Kesari; Adnyana, I Kadek Budi; Damayanti , Ida Ayu Manik; Wulansari , Nadya Treesna; Harditya, Kadek Buja; Adiana, I Nengah; Padmiswari, Anak Agung Istri Mas
Jurnal Abdimas ITEKES Vol 3 No 1 (2023)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Institute Teknologi dan Kesehatan (ITEKES) Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37294/jai.v3i1.504

Abstract

Di Indonesia, prevalensi kejadian hipertensi semakin meningkat dari tahun ke tahun khususnya pada kelompok lansia. Pemahaman terkait hipertensi masih kurang khususnya pada kelompok lansia disertai juga dengan masih kecilnya persentase minum obat secara teratur. Hal ini mampu memberikan komplikasi berat hingga menimbulkan kematian. Berdasarkan hal tersebut, diperlukan intervensi preventif yaitu pemberian penyuluhan mengenai hipertensi pada lansia di Desa Melinggih Kecamatan Payangan Gianyar. Tujuan dari pemberian penyuluhan ini adalah agar para lansia mampu meningkatkan pengetahuannya mengenai penyakit hipertensi dan bagaimana pentalaksanaan yang tepat. Berdasarkan hasil pretest dari 279 responden lansia memiliki pengetahuan kurang tentang hipertensi yaitu sebesar 69.2%. Selanjutnya setelah diberikan penyuluhan, dari hasil posttest sebagian besar responden lansia memiliki pengetahuan yang baik yaitu sebesar 71%. Setelah diberikan intervensi penyuluhan dapat disimpulkan bahwa pengetahuan lansia mengenai hipertensi mengalami peningkatan. Kata Kunci : Pengetahuan, Penyuluhan, Hipertensi, Lansia
Antioxidant Activity Test of Salak Bali Peel Extract (Salacca zalacca var. amboinensis) Against Brain Cells of Male Mice (Mus musculus L.) Induced by Alloxan Padmiswari, Anak Agung Istri Mas; Wulansari, Nadya Treesna; Harditya, Kadek Buja; Megayanti, Sri Dewi
Jurnal Pijar Mipa Vol. 20 No. 1 (2025)
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v20i1.8146

Abstract

This study aims to test the antioxidant activity of salak Bali peel extract (Salacca zalacca var. amboinensis) against brain cells of male mice (Mus musculus L.) exposed to alloxan. Free radicals can trigger damage to brain cells through oxidative mechanisms, which contribute to the development of various neurodegenerative diseases that can damage cells in the body. Salak Bali peel extract contains bioactive compounds such as flavonoids, tannins, and polyphenols that are antioxidant-active. This study was conducted using a completely randomized design (CRD) in mice induced by alloxan at a dose of 150 mg/kg BW (group 1), followed by oral administration of salak Bali peel extract at a dose of 4.2 mg/kg BW (group 2) and 8.4 mg/kg BW (group 3) for 35 days. The control group used in this study was alloxan, which was used to compare the treatment results with conditions without intervention so that the effects of the treatment could be identified more clearly. The results of the antioxidant analysis test using the DPPH method showed an IC50 value of the salak Bali peel extract of 45.5 µg/mL, indicating a high antioxidant capacity. Histopathological analysis revealed that administration of salak Bali peel extract could reduce necrosis in the brain tissue of mice induced by alloxan, suggesting a neuroprotective effect. Therefore, salak Bali peel extract has the potential to be a source of natural antioxidants that can be used to prevent brain damage due to oxidative stress and as a therapy for treating neurodegenerative diseases.
The Administration of a Combined Extract of Gotu Kola (Centella asiatica) and Peppermint (Mentha piperita) Leaves on the Brain Cell Count of Stressed Male Mice (Mus musculus) Padmiswari, Anak Agung Istri Mas; Wulansari, Nadya Treesna; Harditya, Kadek Buja
Jurnal Pijar Mipa Vol. 20 No. 3 (2025)
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v20i3.8843

Abstract

Chronic stress is a condition that can adversely affect the central nervous system, particularly the brain, by causing damage to neurons in the hippocampal region, which plays a crucial role in memory and learning functions. This study aimed to evaluate the neuroprotective effects of a combined extract of gotu kola leaves (Centella asiatica) and peppermint leaves (Mentha piperita) on the number of normal brain cells in male mice (Mus musculus) subjected to chronic stress. A Completely Randomized Design (CRD) was used with five treatment groups: a negative control group (K−) with no stress and no extract, a positive control group (K+) exposed to stress without extract administration, and three treatment groups (P1, P2, and P3) receiving the combined extract at doses of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW, respectively. Stress induction was carried out using the immobilization method for 14 consecutive days, followed by extract administration for 35 days. After the treatment period, hippocampal brain tissue was collected and histologically analyzed using Hematoxylin-Eosin (H&E) staining. To assess the treatment effects, One-Way ANOVA was performed. Significant differences were found, Duncan’s Multiple Range Test (DMRT) was conducted at a 5% significance level (p< 0.05). The results demonstrated that administration of the gotu kola and peppermint extract combination significantly reduced neuronal damage and increased the number of normal brain cells. The P3 group (300 mg/kg BW) exhibited the highest number of normal brain cells and the lowest neuronal damage scores, closely resembling the normal condition in the K− group. Therefore, the combination of gotu kola and peppermint leaf extracts shows potential as a natural neuroprotective agent in mitigating the adverse effects of chronic stress on the brain.
Number of Testicular Cells in Male Mice (Mus musculus L.) Treated with Bali Salak Fruit Extract (Salacca zalacca var. Amboinensis) : Jumlah Sel Testis Mencit Jantan (Mus musculus L.) yang Diberi Ekstrak Buah Salak Bali (Salacca zalacca Var. Amboinensis) Padmiswari, Anak Agung Istri Mas; Wulansari, Nedya Treesna; Indrayoni, Putu
BIO-EDU: Jurnal Pendidikan Biologi Vol. 10 No. 2 (2025): BIO-EDU: Jurnal Pendidikan Biologi - August 2025
Publisher : Jurusan Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v10i2.9329

Abstract

Spermatogenesis is a complex process influenced by various factors, including nutrition and bioactive compounds from natural sources. Salak Bali fruit (Salacca zalacca Var. Amboinensis) is known to contain antioxidant compounds that may support male reproductive health, particularly in enhancing testicular cell count. This study aims to analyze the effect of salak Bali fruit extract on the testicular cell count of male mice (Mus musculus L.). This research employed an experimental design using a Completely Randomized Design (CRD) with 24 healthy male mice divided into four treatment groups. The control group (K) received only distilled water, while the treatment groups were administered salak Bali fruit extract at doses of 200 mg/kg BW (P1), 400 mg/kg BW (P2), and 600 mg/kg BW (P3) for 35 days. Data analysis was conducted using a one-way ANOVA test, followed by a post hoc test if a significant difference was found (p < 0.05). The results indicated that the treatment groups exhibited a higher average testicular cell count compared to the control group. The most optimal increase was observed in the group receiving a 600 mg/kg BW dose (P3). This suggests that the bioactive compounds in salak Bali fruit may play a role in enhancing the spermatogenesis process.
Comparison of the Effects of a Combination of Centella asiatica and Mentha piperita Leaf Extracts on the Number of Normal Brain Cells in Male Mice (Mus musculus) Padmiswari, Anak Agung Istri Mas; Antari, Ni Wayan Sukma; Gandamayu, Ida Bagus Maha; Adiana, I Nengah
Jurnal Pijar Mipa Vol. 20 No. 6 (2025)
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v20i6.9998

Abstract

Chronic stress is known to cause neuronal damage in the hippocampus, particularly in the CA1 and CA3 areas, which play a crucial role in memory and learning. One way to prevent this damage is by using natural ingredients with neuroprotective properties. This study aimed to compare the effects of Centella asiatica and Mentha piperita leaf extracts, either singly or in combination, on the number of normal neurons in the hippocampus of male mice exposed to stress. Twenty-five male mice were divided into five treatment groups: K− (control without treatment), K+ (stress without extract), P1 (stress + Centella asiatica extract), P2 (stress + Mentha piperita extract), and P3 (stress + combination of Centella asiatica and Mentha piperita). Stress was induced using a restraint stress method, while the extracts were administered orally at the prescribed dosage. After the treatment period, the hippocampus was removed, and histological slides were prepared with Hematoxylin-Eosin staining to count the number of normal neurons in the CA1 and CA3 areas. The results showed that the K+ group experienced a significant decrease in the number of normal neurons compared to the K− group. Administration of gotu kola (P1) and mint (P2) extracts increased the number of neurons compared to K+, but not to the same extent as K−. The combination of Centella asiatica and Mentha piperita extracts (P3) showed the most optimal effect, with the number of normal neurons approaching that of the negative control group. These findings indicate that the combination of Centella asiatica and Mentha piperita has synergistic potential in protecting hippocampal neurons from stress-induced damage. Further research is needed to explore the molecular mechanisms underlying this neuroprotective effect.
Liver Histology of Male Rat (Rattus norvegicus) Feed L-Carnitine Supplement Padmiswari, Anak Agung Istri Mas; Indrayoni, Putu; Wulansari, Nadya Treesna
Jurnal Pijar Mipa Vol. 19 No. 4 (2024): July 2024
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v19i4.6900

Abstract

Obesity is a condition where the body is overweight due to fat accumulation, each exceeding 20-25% of body weight for men and women. L-carnitine is a nutrient that plays a role in transporting fat to the mitochondria to be burned and converted into energy for the body. Sufficient L-carnitine allows the body to burn fat at optimal levels. This research aims to determine the side effects of fed L-carnitine supplements against liver histology. This study used a completely randomized design (CRD) with four groups comprising six albino rats. The treatments were fed orally by distilled water 1 ml/day (Control), P1 fed by L-carnitine supplement dose 100 mg/kg body weight orally 1ml/day, P2 fed by L-carnitine supplement dose 150 mg/kg body weight orally 1ml/day, P3 fed by L-carnitine supplement dose 200 mg/kg body weight orally 1ml/day. Treatments were given to albino rats every day for 90 days. Variables observed measured were lipid degeneration, central vein dilation, and pyknotic nuclei. Data were analyzed with the statistical program (SPSS 22.0 for Windows) with One Way ANOVA. The results showed that feeding L-carnitine supplements in high doses for long periods can damage the liver cells of male rats. The microscopic liver damage statistical analysis showed significant differences (P<0.05) between the control and treatment groups.
Gambaran Histologi Ovarium dan Fluktuasi Hormon Estrogen pada Mencit Betina (Mus musculus L.) yang diberi Ekstrak Buah Zuriat (Hyphaene thebaica) sebagai Antioksidan Alami Antari, Ni Wayan Sukma; Damayanti, Ida Ayu Manik; Padmiswari, anak agung istri mas; Harditya, Kadek Buja
MAHESA : Malahayati Health Student Journal Vol 3, No 4 (2023): Volume 3 Nomor 4 (2023)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.134 KB) | DOI: 10.33024/mahesa.v3i4.10260

Abstract

ABSTRACT Hyphaene thebaica belongs to the Palmae family which is often referred to as zuriat fruit. Hyphaene thebaica has properties as an antimicrobial, antioxidant, and antidiabetic. This study aims to determine the effect of zuriat extract on the number of follicles on the ovaries in female mice (Mus musculus) given exposure to cigarette smoke. This research have conducted for 6 months from January to June 2021. This type of research was pure experimental (true experimental) with a post test only control group design approach. This study was conducted by giving zuriat fruit extract as treatment for 42 days to female mice. The sample in this study was calculated using the Federer formula (1991). After treatment for 42 days, observations were made on ovarian histology which included: a. primary follicles, b. secondary follicles c. tertiary follicles and estrogen hormone. The research data were processed using a computer statistical program (SPSS 22.0 for Windows) using the OneWay Anova test. The results showed that the administration of zuriat fruit extract can increase the number of follicles in the ovary and increase estrogen hormone.  Keywords: (Mus musculus L.), free radicals, smoking, Hyphaene thebaica  ABSTRAK Hyphaene thebaica termasuk dalam keluarga Palmae yang sering disebut dengan buah zuriat. Hyphaene thebaica memiliki khasiat sebagai antimikroba, antioksidan, dan antidiabetes Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak zuriat terhadap jumlah folikel pada ovarium pada mencit betina (Mus musculus) yang diberi paparan asap rokok. Penelitian ini akan dilaksanakan selama 6 bulan pada bulan Januari sampai dengan Juni 2021. Jenis penelitian ini adalah eksperimental murni (true experimental) dengan pendekatan post test only control group design. Penelitian ini dilakukan dengan memberikan ekstrak buah zuriat sebagai perlakuan selama 42 hari pada mencit betina. Sampel yang akan digunakan dalam penelitian ini dihitung menggunakan rumus Federer (1991). Setelah dilakukan pemberian perlakuan selama 42 hari selanjutnya dilakukan pengamatan pada histologi ovarium yang meliputi: a. folikel primer, b. folikel sekunder c. folikel tersier dan hormone estrogen. Data hasil penelitian diolah menggunakan program statistik komputer (SPSS 22.0 for Windows) dengan menggunakan uji One Way Anova. Hasil penelitian menunjukan bahwa pemberian ekstrak buah zuriat dapat meningkatkan jumlah folikel pada ovarium dan meningkatkan hormone estrogen. Kata Kunci: (Mus musculus L.), Radikal Bebas, Rokok, Hyphaene Thebaica
PENGARUH PEMBERIAN EKSTRAK KOMBINASI DAUN PEGAGAN (Centella asiatica) DAN DAUN MINT (Mentha arvensis) TERHADAP JUMLAH SEL TESTIS PADA MENCIT JANTAN (Mus musculus) Padmiswari, Anak Agung Istri Mas; Wulansari, Nadya Treesna; Harditya, Kadek Buja
Berita Biologi Vol 24 No 2 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.11234

Abstract

Stres oksidatif merupakan salah satu faktor yang dapat menurunkan fungsi sistem reproduksi jantan, terutama melalui kerusakan pada sel-sel germinal di testis. Upaya preventif yang dapat dilakukan untuk mengatasi kondisi ini adalah dengan pemberian senyawa alami yang memiliki aktivitas antioksidan tinggi, seperti flavonoid, alkaloid, dan tanin yang banyak terkandung dalam tanaman herbal. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak kombinasi daun pegagan (Centella asiatica) dan daun mint ((Mentha arvensis) terhadap jumlah sel testis dan berat testis mencit jantan (Mus musculus) yang dipapar stres. Penelitian menggunakan rancangan acak lengkap dengan lima kelompok perlakuan: kontrol negatif (K−), kontrol positif (K+), dan tiga kelompok perlakuan dengan dosis ekstrak kombinasi 100 mg/kgBB (P1), 200 mg/kgBB (P2), dan 300 mg/kgBB (P3). Uji fitokimia menunjukkan bahwa ekstrak kombinasi daun pegagan dan daun mint mengandung flavonoid, alkaloid, saponin, tanin, dan triterpenoid. Aktivitas antioksidan ekstrak kombinasi daun pegagan dan daun mint diuji menggunakan metode DPPH dengan hasil nilai IC50 tergolong kuat. Parameter yang diamati meliputi berat testis serta jumlah sel spermatogonium, spermatosit, dan spermatid. Hasil penelitian menunjukkan bahwa pemberian ekstrak kombinasi secara signifikan meningkatkan (p < 0,05) berat testis dan jumlah sel testis pada kelompok perlakuan dibandingkan dengan kelompok yang hanya dipapar stres (K+). Kelompok P3 memberikan hasil paling optimal dalam menjaga struktur dan fungsi yang dapat dilihat dari histologi testis. Analisis data dilakukan dengan SPSS versi 22.0 menggunakan uji Kolmogorov-Smirnov untuk normalitas, uji Levene untuk homogenitas, dilanjutkan dengan ANOVA satu arah dan uji lanjutan Duncan (DMRT). Berdasarkan hasil tersebut, dapat disimpulkan bahwa  ekstrak kombinasi daun pegagan dan mint berpotensi sebagai agen fitoterapi dalam mengurangi dampak stres oksidatif terhadap sistem reproduksi jantan.
UJI AKTIVITAS ANTIOKSIDAN KOMBINASI EKSTRAK KUNYIT (Curcuma longa) DAN GINSENG (Panax ginseng) SEBAGAI BAHAN MINUMAN HERBAL ALAMI Padmiswari, Anak Agung Istri Mas; Wulansari, Nadya Treesna; Harditya, Kadek Buja
INDIGENOUS BIOLOGI : JURNAL PENDIDIKAN DAN SAINS BIOLOGI Vol 8 No 2 (2025): INDIGENOUS BIOLOGI : JURNAL PENDIDIKAN DAN SAINS BIOLOGI
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v8i2.733

Abstract

Penelitian ini dilatarbelakangi oleh meningkatnya paparan radikal bebas yang memicu stres oksidatif dan penyakit degenerative akibat faktor lingkungan dan makanan. Kunyit (Curcuma longa) dan ginseng (Panax ginseng) diketahui memiliki aktivitas antioksidan, namun informasi mengenai efektivitas kombinasinya dalam bentuk minuman herbal masih terbatas. Penelitian ini bertujuan mengevaluasi aktivitas antioksidan kombinasi ekstrak kunyit dan ginseng menggunakan metode DPPH. Metode penelitian yang digunakan adalah eksperimen skala laboratorium. Desain penelitian ini adalah rancangan acak lengkap. Hasil penelitian menunjukkan bahwa ekstrak tunggal kunyit dan ginseng memiliki aktivitas antioksidan sedang dengan IC₅₀ masing-masing 76,40 µg/mL dan 82,75 µg/mL. Kombinasi ekstrak memberikan aktivitas lebih tinggi, terutama rasio 1:1 dengan IC₅₀ 43,25 µg/mL, yang tergolong sangat kuat dan berbeda nyata (p < 0,05) dibanding perlakuan lain. Kesimpulannya, kombinasi kunyit–ginseng, khususnya rasio 1:1, menunjukkan efek sinergis dan berpotensi dikembangkan sebagai bahan aktif minuman herbal fungsional.
EFEK NEUROPROTEKTIF EKSTRAK DAUN PEGAGAN (Centella asiatica) TERHADAP JUMLAH SEL NEURON HIPPOCAMPUS PADA MENCIT JANTAN (Mus musculus) YANG DIPAPAR STRES Padmiswari, Anak Agung Istri Mas; Wulansari, Nadya Treesna; Harditya, Kadek Buja
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.13391

Abstract

Stres merupakan salah satu faktor utama yang berkontribusi terhadap neurodegenerasi, menurunkan kelangsungan hidup sel otak, dan mengurangi kepadatan neuron di hippocampus. Senyawa alami yang memiliki sifat antioksidan dan neuroprotektif, seperti Centella asiatica (pegagan), menarik perhatian sebagai agen terapeutik potensial. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun pegagan terhadap berat otak dan jumlah neuron hippocampus mencit jantan (Mus musculus) yang dipaparkan stres kronis. Sebanyak 25 ekor mencit dibagi menjadi lima kelompok: kontrol negatif (K–), kontrol positif (K+), serta kelompok perlakuan yang diberikan ekstrak pegagan dengan dosis 100 mg/kg (P1), 200 mg/kg (P2), dan 300 mg/kg (P3). Berat otak dan kepadatan neuron hippocampus diukur setelah perlakuan. Hasil penelitian menunjukkan bahwa stres kronis secara signifikan menurunkan berat otak dan jumlah neuron hippocampus pada kelompok K+ dibandingkan K–. Sebaliknya, pemberian ekstrak Centella asiatica mampu memperbaiki kedua parameter tersebut secara bergantung dosis, dengan peningkatan tertinggi pada kelompok P3. Temuan ini sejalan dengan penelitian sebelumnya yang menunjukkan potensi antioksidan, antiinflamasi, dan neurogenik dari Centella asiatica, yang diduga bekerja melalui regulasi penanda stres oksidatif, modulasi poros hipotalamus-hipofisis-adrenal (HPA), serta peningkatan faktor neurotropik. Kesimpulannya, ekstrak Centella asiatica memberikan efek protektif terhadap kerusakan otak akibat stres, sehingga berpotensi dikembangkan sebagai agen neuroprotektif alami. Penelitian lebih lanjut diperlukan untuk mengeksplorasi mekanisme molekuler, keamanan jangka panjang, serta efektivitas klinis pada manusia.
Co-Authors A.A.S. A. Sukmaningsih Adiana, I Nengah Adnyana, I Kadek Budi Anggaraeni, Komang Rosa Tri Dira, Made Asmarani Dwi Arymbhi Sanjaya Gandamayu, Ida Bagus Maha Gede Surya Indrawan I Wayan Kasa Ida Ayu Manik Damayanti Kadek Buja Harditya Nadya Treesna Wulansari Nadya Treesna Wulansari Nadya Treesna Wulansari Nadya Treesna Wulansari Ngurah Intan Wiratmini Ni Putu Adriani Astiti Ni Wayan Kesari Dharmapatni Ni Wayan Sukma Antari Putu Indrayoni Sri Dewi Megayanti Strisanti, Ida Ayu Suptika Wulansari , Nadya Treesna Wulansari, Nedya Treesna