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All Journal HAYATI Journal of Biosciences International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Bumi Lestari Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology JURNAL HUBUNGAN INTERNASIONAL Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Bawal : Widya Riset Perikanan Tangkap Folia Medica Indonesiana SIMBIOSIS Jurnal Biologi Udayana

Ngurah Intan Wiratmini

Program Studi Biologi Fakultas MIPA Universitas Udayana, Bali

Author-ID : 287691

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Neuroscience Public Health

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Analisis Perubahan Respons Amerika Serikat Terhadap Ancaman Terorisme Islam: Desekuritisasi Periode Obama Wiratmini, Ni Putu Eka; Sushanti, Sukma; Kawitri Resen, Putu Titah
DIKSHI (DISKUSI ILMIAH KOMUNITAS HUBUNGAN INTERNASIONAL) Vol 1 No 2 (2018)
Publisher : Program Studi Hubungan Internasional FISIP Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.157 KB)

ABSTRACT The process of desecuritization of Islamic terrorism becomes an interesting thing to be studied in the study of International Relations. This study attempted to analyze the process of Obama in conducting the desecuritization of terrorism through his speech to the Islamic community and the world. The research used descriptive qualitative research method with two concepts, namely: speech act from Nicholus Onuf which is explain the built process of rules through language construction and desecuritization by Barry Buzan that’s explain how threats transfer into the public sphere. Both concepts can help to analyze the process of Obama conducting Islamic terrorism desecuritization. This desexuritization is related to Obama identity that’s close by the culture and environment of third world countries. This makes him have a better understanding of Islam than his predecessor. The analytical technique used in this study is to interpret the data through focusing Obama's discourse or speech. Keywords: Terrorism, Desecuritization, Speech Act, United States

THE DEVELOPMENT OF OVARIAN FOLLICLE CELLS AND CORPUS LUTEUM OF MICE (Mus musculus) SWISS WEBSTER GIVEN Leucaena leucocephala LEAF EXTRACT Ngurah Intan Wiratmini; Ni Wayan Sudatri; Iriani Setyawati
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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This research was conducted to determine the effect of Leucaena leucocephala leaf extract on the development of ovarian follicle cells of mice (Mus musculus). Twenty four female mice, 10 weeks old, were divided into 4 groups. The control group (P0) were given 0.9% NaCl and the treatment group P1, P2 and P3 were given 0.5, 1 and 1.5 g/ kg bw of Leucaena leaf extract, respectively. The treatments were administered daily for 15 days by gavage. After the treatment ended, all mice were dissected to collect the ovaries. Histological preparation of the ovaries used paraffin method and Hematoxylin-Eosin staining. Observations were made on the number of primary, secondary, and tertiary oocytes, and also De Graaf follicles and corpus luteum. Data were statistically analyzed using One Way ANOVA method. The results showed there were no significantly differences (P>0.05) on the number of primary, secondary, and tertiary oocytes, as well as De Graaf follicles and corpus luteum among the control and treatment groups. The mimosine content of Leucaena leaf extract was 0.87% (less than 1%) so it could not inhibit the secretion of Follicle Stimulating hormone by the pituitary gland. The doses of Leucaena leaf extract used in this research did not significantly affect the follicles development of mice ovaries. In this study, the mimosine content of Leucaena leaf extract decreased 91.8%.

THE EFFECTS OF DIETARY INCLUSION OF DETOXIFIED Leucaena leucocephala LEAF MEAL ON THYROIDAL ACTIVITY OF RATS DURING GESTATION-LACTATION PERIOD Ngurah Intan Wiratmini; Inna Narayani; Ni Luh Eka Setiasih
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Leucaena leucocephala provides the highest amount of proteins compared to other greens. However, its use is restricted due to the content of mimosine, an antinutrition non-protein amino acid known to be toxic to animals. This study aimed to observe the effect of administration of more than 10% of processed Leucaena leucocephala leaf meal to the level of T3, T4 and the thyroid histopathology. In this study, Leucaena leucocephala leaves were soaked in distilled water for 12 hours. The processed of Leucaena leucocephala leaf meal was made into pellets containing 0%, 7.5%, 15%, and 22.5% leaf meal of total feeds respectively, and was fed to the rats during pregnancy and lactation. The level of triiodothyronine (T3) and thyroxine (T4), and the thyroid histological features were the parameters observed. The collected data were statistically analyzed by SPSS 20.0 for Windows using One-way ANOVA followed by Duncan Multiple Range Test to observe any significant difference among the 4 treatment groups (?=5%). Meanwhile, the presence of hyperplastic cells and follicle lumens filled with vesicles and colloids was descriptively analyzed by means of available literatures. The analysis of T3 and T4 level revealed that there was no any significant difference between the control and treatment groups. The epithelial cells of thyroid follicles in the treatment groups of P1, P2 and P3 showed hyperplasia and were detached from their respective basal membranes.

PELATIHAN PENGADAAN DAN PENGAWETAN BAHAN PRAKTIKUM BIOLOGI UNTUK GURU SMP BIDANG IPA DI KECAMATAN KUBUTAMBAHAN KABUPATEN BULELENG Intan Wiratmini
Buletin Udayana Mengabdi Vol 19 No 3 (2020): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.641 KB)

Kendala yang dihadapi dalam mengajar IPA khususnya Biologi adalah tidak tersedianya bahan-bahan praktikum terutama untuk anatomi hewan. Selain karena membutuhkan biaya yang tidak sedikit, pengetahuan untuk menyiapkan bahan praktikum yang murah dan efisien belum dikuasai. Tujuan dari kegiatan pengabdian ini adalah memberikan pelatihan/praktikum langsung kepada guru-guru SMP bidang IPA di Kecamatan Kubutambahan Kabupaten Buleleng tentang cara membedah anatomi hewan coba (mamalia), pembuatan herbarium, insektarium dan pengawetannya sehingga bahan-bahan praktikum tersebut selalu tersedia di laboratorium. Metode pengabdian yang digunakan adalah PRA (Participatory Rural Apraisal), pelaksanaan pelatihan dilakukan dengan ceramah, praktikum dan evaluasi. Kegiatan pelatihan dilaksanakan di SMPN1 Kubutambahan dengan jumlah peserta 28 orang guru bidang IPA dari 7 SMPN di Kecamatan Kubutambahan. Hasil akhir dari kegiatan pengabdian menghasilkan beberapa produk, antara lain herbrium, insektarium dan awetan basah hewan mamalia (mencit jantan dan betina)

MAKROZOOBENTHOS PANTAI PERERENAN (KABUPATEN BADUNG): JENIS, STATUS, DAN MANFAATNYA BAGI MASYARAKAT Ngurah Intan Wiratmini; Joko Wiryatno; A.A.G. Raka Dalem
Bumi Lestari Journal of Environment Vol 8 No 2 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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The research was undertaken on Pererenan beach in the Regency of Badung, Bali Province, between September and December, 2006. The study sites occur close to the downstream of Bausan creek, the location of which were part of ”Subak Kedungu” irrigation system (east of the creek) and ”Subak Munggu Tegal Lantang” irrigation system (west of the creek). Samples were taken on low tides periods of the intertidal zones of the beach. Samples were stratified randomly taken on three kinds of substrates: on sand and coral substrates on the west, sand substrates in the middle and hard sandstone on the east, each of which consist of five quadrates of 40 cm x 40 cm. Results of this research showed that on the site have been successfully identified 17 species of macrozoobenthic fauna. None of them were classified as protected species. The macrozoobenthic fauna on Parerenan beach had a medium level of index of diversity (IK = 2,598). This meant that, the beach ecosystem in Pererenan was in a stable condition with a medium level of diversity. From the benefit and problems related to their occurrence, it can be described that macrozoobenthic organisms on this study sites, some can be consumed. Besides, there were also some species which dangerous to human because they can cause bleeding, or dangerous hurt caused by sharp needles.

BIOLOGICAL ASPECTS, CATCHING ASPECTS AND FISHING GROUND OF EASTERN LITTLE TUNA OR KAWAKAWA(Euthynnusaffinis (Cantor, 1849)) BASED ON THE FISHING GEAR AT WPP 572 Gussasta Levi Arnenda; Bram Setyadji; Ngurah Intan Wiratmini; I Made Sara Wijana
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 16, No 3 (2020): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.16.3.%p

Eastern little tuna (Euthynnus affinis) is the main catch in WPP572. The caught E. affinis are still in their infancy. This research aims is to know the biological aspects, the fishing aspects and the distribution of the fishing gear per fishing gear, as a consideration for sustainable fisheries management. The research was carried out during the years 2019-2020. Taking samples for gonad observation was carried out by direct survey to the location in February, May, July and October 2019. The measurement of fish length was carried out in stratified random. Catching data were retrieved by requesting secondary data and interviews with related parties. The distribution of fishing grounds is mapped using the QGIS 3.4, the spatial distribution of catches is mapped. This study showed the size structure of E. affinis on purse seine, troll line and encircling gill netfishing gear from 16-55 cm FL, while on the boat/raft lift net it was 21-50 cmFL. These fish growth's is positive allometric on purse seine and encircling gillnet fishing gear, negative allometric on boat/raft lift net fishing gear, and isomeric on troll line. The sex ratio is in the ratio 1: 1.15 and balanced. Based on the maturity level, the gonads were mature in July and October and immature in February and May. Gonads ripened for the first time at a size of 42.032 cmFL. The distribution of the purse seine catchment areas at 3°-6°N and 93°-96°E, and 3°-6°S and 94°-95°E. Boat/raft lift net at 0°-3°S and 95° -101°E. Troll line 4°-6°N and 94°-95 E. Encircling gillnet 0°-2°S and 99°-101°E.

PENANAMAN DAN PEMANFAATAN TANAMAN OBAT KELUARGA SEBAGAI MINUMAN IMMUNE BOOSTER M. Pharmawati; L.P. Wrasiati; I.M.A.S. Wijaya; N.I. Wiratmini
Buletin Udayana Mengabdi Vol 21 No 3 (2022): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

The immune system is important in dealing with foreign objects that enter the body. A good body defense system is very important in the existence of the corona virus disease 19 (COVID-19) pandemic caused by the SARS-CoV2. The immune system can be improved by consuming foods with balanced nutrition and various kinds of herbs, in the form of herbal medicine. Jamu is a product derived from medicinal plants that can function as an immune booster. The government has campaigned for the planting of family medicinal plants (TOGA) as an effort to maintain health. In Budaga Village, Klungkung Regency, several families have planted medicinal plants, but the types of are still limited and the processing carried out is still very simple, namely brewing the leaves or plant rhizomes. The purpose of this activity was to introduce cultivation techniques of various kinds of medicinal plants, as well as to train member of PKK of Budaga Village in processing medicinal plants into herbal jamu immune booster. The activity was held on September 1, 2021, attended by representatives of PKK in Budaga Village. The methods used are lectures, demonstrations and direct practice of seeding and planting medicinal plants and practice of preparing jamu. The results of this activity are that the community can cultivate medicinal plants and able to prepare jamu following recipe from Kementerian Kesehatan RI. This activity produced seeds of medicinal plants ginger, red ginger, Piper betle, lemongrass, turmeric, mint and three herbal products, namely jamu gula asam, kunyit asam and temulawak. Keywords: Budaga Village, immune booster, jamu, PKK, TOGA

PENDAMPINGAN PENGADAAN BAHAN PRAKTUKUM ZOOLOGI DAN BIOTEKNOLOGI BAGI GURU SMA DI KABUPATEN KLUNGKUNG N.I. Wiratmini; A.A.S. Sukmaningsih; I. Narayani; M. Pharmawati
Buletin Udayana Mengabdi Vol 21 No 3 (2022): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Advances in molecular biology has brought a new branch of multidiscipline study named biotechnology. To comprehend the complex nature of concepts in biology, practical and hands-on approaches are often required. This activity was a collaboration between Biology Teachers Working Group (MGMP Biologi) in Klungkung Regency with university as the facilitator, and aimed to train senior high school biology teachers in compiling learning modules as well as supplying them with the appropriate materials required in simple practical sessions, such as those in animal physiology and reproduction, as well as biotechnology. The event was held successfully, attended by total of 20 biology teachers from six senior high schools (SMA) in Klungkung District, Klungkung Regency, started by introductory lectures and followed by practical sessions on how to perform mice vaginal and sperm smear, human blood smear, and DNA extraction of plant tissues. Keywords: practicals, zoology, biotechnology, senior high school students

KLASIFIKASI TINGKAT KEMATANGAN GONAD DAN PEMIJAHAN IKAN TONGKOL KOMO BETINA (EUTHYNNUS AFFINIS (CANTOR, 1849)) YANG DIDARATKAN DI KEDONGANAN- BALI arnenda, gussasta levi; Wiratmini, Ngurah Intan; Wijana, I Made Sara
BAWAL Widya Riset Perikanan Tangkap Vol 15, No 3 (2023): (DESEMBER) 2023
Publisher : Politeknik Kelautan dan Perikanan Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/bawal.15.3.2023.132-146

Wilayah Pengelolaan Perikanan Republik Indonesia di Samudera Hindia dengan kode WPP-RI 572 dan 573 kaya akan potensi sumber daya ikan yang didominasi oleh ikan Tongkol Komo (Euthynnus affinis (Cantor, 1849). Pemanfaatan ikan Tongkol Komo telah melampaui batas optimal dengan tekanan penangkapan yang tinggi. Biologi reproduksi Tongkol Komo perlu diketahui untuk memastikan keberadaannya di alam. Tujuan dari penelitian ini adalah Tahap Kematangan Gonad (TKG) dan pemijahan. Penelitian dilaksanakan pada bulan Februari, April, Juni hingga Desember 2020. Pengumpulan data dilakukan melalui pengamatan langsung di Pangkalan Pendaratan Ikan (PPI) Kedonganan. Teknik pengambilan sampel yang digunakan dalam penelitian ini adalah purposive stratified sampling. Analisis histologi di Laboratorium Penelitian Perikanan Tuna, Denpasar. Hasil yang diperoleh bahwa pola pertumbuhan tuna komo didominasi oleh pola alometrik positif dengan sebaran panjang 20-65cmFL. Ukuran pertama kali matang gonad (Lm) untuk betina 44,07 cmFL. Tingkat Kematangan Gonad (TKG) pada klasifikasi makroskopis dan mikroskopis perkembangan gonad didominasi oleh ikan yang belum matang gonad. Musim pemijahan pada bulan Agustus dan Desember.

Anti-Inflammatory Activity of Phyllanthus niruri L. Leaf Extract: In Vitro Study on RAW 264.7 Macrophage Cells and In Silico Analysis Intan Wiratmini, Ngurah; Pharmawati, Made; Sukmaningsih, Anak Agung Sagung Alit; Joko Raharjo, Sentot; Widowati, Wahyu
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.287-299

Phyllanthus niruri L. is a medicinal plant recognized for its wide range of therapeutic benefits, particularly its anti-inflammatory properties. This research focused on assessing the impact of P. niruri leaf extract on pro-inflammatory cytokines in vitro using RAW 264.7 macrophage cells, analyze its phytochemical composition, and validate its potential anti-inflammatory mechanisms through molecular docking studies. The extract was analyzed for its phytochemical composition using LC-MS, revealing the presence of sesquiterpene glycosides, flavonoid glycosides, and tannins among the identified compounds. Cell viability tests showed that the extract was not toxic to the cells at concentrations up to 50 µg/ml. Treatment with the extract significantly reduced the levels of pro-inflammatory cytokines TNF-α and IL-1β in LPS-induced RAW 264.7 cells, with the most effective concentration being 50 µg/ml. Molecular docking studies further supported the anti-inflammatory potential of the extract by demonstrating its ability to inhibit COX-2 and TNF-alpha. These findings suggest that P. niruri leaf extract has promising anti-inflammatory properties and needs further investigation as a potential treatment for inflammatory diseases. The in silico study provided insights into the molecular interactions between the extract's compounds and inflammatory targets, supporting its anti-inflammatory mechanism of action.

Co-Authors A.A.S. A. Sukmaningsih AA Istri Mas Padmiswari Anak Agung Gde Raka Dalem Apriliani, Sinta Arnenda, Gussasta Levi Bram Setyadji Dessy Sukma Wirastuti Elfira Dzikri Ashfahani Elrade Rofaani G.A.A Rina Febrina Gussasta Levi Arnenda I Gede Wiranatha I GUSTI AYU MANIK ERMAYANTI I Made Sara Wijana I Made Sara Wijana I Putu Ari Wijana Dipa I Wayan Andi Yoga Kurniawan I Wayan Kasa I.M.A.S. Wijaya Ida Ayu Nopiari Ida Ayu Ratih Purnama Dewi Inna Narayani IRIANI SETYAWATI Iriani Setyawati Jaqueline Sudiman Job Nico Subagio Joko Raharjo, Sentot Joko Wiryatno Luh Putu Wrasiati MADE PHARMAWATI Maria Antonia Margaretha Ferandez Maria Antonia Margaretha Fernandez Mery Wintari Muyasaroh, Chanif Lutfiyati Ni Desak Made Aprilia Dewi Ni Komang Tia Pramesti Ni Luh Eka Setiasih Ni Luh Suriani Ni Made Rai Suarni Ni Made Suartini Ni Putu Adriani Astiti Ni Putu Ayu Dewi Wijayanti Ni Putu Sri Risa Dewi Ni Wayan Adya Puttry Purna Yogini Ni Wayan Sudatri Putu Titah Kawitri Resen Revael Sihombing Salsabillah Rahma SANG KETUT SUDIRGA shanon lestari Sistra, Kanigara Anupama Siti Masnunah Sri Sulastri Sukma Sushanti Sukmaningsih, A. A.S.A. Wahyu Puji Lestari WAHYU WIDOWATI Widia Meliawati

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