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All Journal HAYATI Journal of Biosciences International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology SIMBIOSIS JURNAL HUBUNGAN INTERNASIONAL Folia Medica Indonesiana Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Bawal : Widya Riset Perikanan Tangkap
Ngurah Intan Wiratmini
Program Studi Biologi Fakultas MIPA Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Public Health Other

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Analisis Perubahan Respons Amerika Serikat Terhadap Ancaman Terorisme Islam: Desekuritisasi Periode Obama Wiratmini, Ni Putu Eka; Sushanti, Sukma; Kawitri Resen, Putu Titah
DIKSHI (DISKUSI ILMIAH KOMUNITAS HUBUNGAN INTERNASIONAL) Vol 1 No 2 (2018)
Publisher : Program Studi Hubungan Internasional FISIP Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.157 KB)

Abstract

ABSTRACT The process of desecuritization of Islamic terrorism becomes an interesting thing to be studied in the study of International Relations. This study attempted to analyze the process of Obama in conducting the desecuritization of terrorism through his speech to the Islamic community and the world. The research used descriptive qualitative research method with two concepts, namely: speech act from Nicholus Onuf which is explain the built process of rules through language construction and desecuritization by Barry Buzan that’s explain how threats transfer into the public sphere. Both concepts can help to analyze the process of Obama conducting Islamic terrorism desecuritization. This desexuritization is related to Obama identity that’s close by the culture and environment of third world countries. This makes him have a better understanding of Islam than his predecessor. The analytical technique used in this study is to interpret the data through focusing Obama's discourse or speech. Keywords: Terrorism, Desecuritization, Speech Act, United States
THE DEVELOPMENT OF OVARIAN FOLLICLE CELLS AND CORPUS LUTEUM OF MICE (Mus musculus) SWISS WEBSTER GIVEN Leucaena leucocephala LEAF EXTRACT Ngurah Intan Wiratmini; Ni Wayan Sudatri; Iriani Setyawati
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.15 KB)

Abstract

This research was conducted to determine the effect of Leucaena leucocephala leaf extract on the development of ovarian follicle cells of mice (Mus musculus). Twenty four female mice, 10 weeks old, were divided into 4 groups. The control group (P0) were given 0.9% NaCl and the treatment group P1, P2 and P3 were given 0.5, 1 and 1.5 g/ kg bw of Leucaena leaf extract, respectively. The treatments were administered daily for 15 days by gavage. After the treatment ended, all mice were dissected to collect the ovaries. Histological preparation of the ovaries used paraffin method and Hematoxylin-Eosin staining. Observations were made on the number of primary, secondary, and tertiary oocytes, and also De Graaf follicles and corpus luteum. Data were statistically analyzed using One Way ANOVA method. The results showed there were no significantly differences (P>0.05) on the number of primary, secondary, and tertiary oocytes, as well as De Graaf follicles and corpus luteum among the control and treatment groups. The mimosine content of Leucaena leaf extract was 0.87% (less than 1%) so it could not inhibit the secretion of Follicle Stimulating hormone by the pituitary gland. The doses of Leucaena leaf extract used in this research did not significantly affect the follicles development of mice ovaries. In this study, the mimosine content of Leucaena leaf extract decreased 91.8%.
THE EFFECTS OF DIETARY INCLUSION OF DETOXIFIED Leucaena leucocephala LEAF MEAL ON THYROIDAL ACTIVITY OF RATS DURING GESTATION-LACTATION PERIOD Ngurah Intan Wiratmini; Inna Narayani; Ni Luh Eka Setiasih
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.757 KB) | DOI: 10.24843/IJBB.2018.v05.i02.p02

Abstract

Leucaena leucocephala provides the highest amount of proteins compared to other greens. However, its use is restricted due to the content of mimosine, an antinutrition non-protein amino acid known to be toxic to animals. This study aimed to observe the effect of administration of more than 10% of processed Leucaena leucocephala leaf meal to the level of T3, T4 and the thyroid histopathology. In this study, Leucaena leucocephala leaves were soaked in distilled water for 12 hours. The processed of Leucaena leucocephala leaf meal was made into pellets containing 0%, 7.5%, 15%, and 22.5% leaf meal of total feeds respectively, and was fed to the rats during pregnancy and lactation. The level of triiodothyronine (T3) and thyroxine (T4), and the thyroid histological features were the parameters observed. The collected data were statistically analyzed by SPSS 20.0 for Windows using One-way ANOVA followed by Duncan Multiple Range Test to observe any significant difference among the 4 treatment groups (?=5%). Meanwhile, the presence of hyperplastic cells and follicle lumens filled with vesicles and colloids was descriptively analyzed by means of available literatures. The analysis of T3 and T4 level revealed that there was no any significant difference between the control and treatment groups. The epithelial cells of thyroid follicles in the treatment groups of P1, P2 and P3 showed hyperplasia and were detached from their respective basal membranes.
Uji Efektivitas Vitamin C sebagai Neuroprotektor pada Mencit (Mus musculus) yang Diinduksi Akrilamida Salsabillah Rahma; Ngurah Intan Wiratmini; Ni Wayan Sudatri
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p08

Abstract

Acrylamide is a contaminant compound found in several types of food containing carbohydrates and processed food through frying or roasting at high temperatures. Acrylamide compounds could cause the formation of oxidative stress which could lead to neurotoxicity. This oxidative stress could be minimized by administering the antioxidant vitamin C. This study was conducted to determine the mice cerebral cortex histology, the mice cerebellum histology induced by acrylamide and vitamin C supplementation, and the most effective dose as a neuroprotector. This study used a completely randomized design (CRD) with 4 groups, namely K + (acrylamide 12.5 mg / kg BW); K- (NaCl 0.9%); P1 (vitamin C dose of 0.26 mg/animal /day + acrylamide dose of 12.5 mg/kg BW); and P2 ((vitamin C dose of 0.52 mg/animal/day + acrylamide dose of 12.5 mg / kg BW) and 6 repetitions so that the number of mice used was 24. The acrylamide and vitamin C solutions were administered orally, and acrylamide given for 20 days, while vitamin C was given 3 days before acrylamide induction until day 20. Mice were dissected at day 21, brain organs were collected for histological preparations by the paraffin method and Hematoxylin-Eosin staining. The qualitative data obtained were analyzed by One Way ANOVA and continued with the Duncan test. The parameters observed were necrosis in pyramid cells and glial cells. The results showed that vitamin C at dose of 0.52 mg/animal/day significantly repaired the damage in pyramid cells and glial cells induced by acrylamide.
Keragaman Genetik dan Hubungan Kekerabatan Ayam Lokal Berdasarkan Penanda RAPD Mery Wintari; Made Pharmawati; Ngurah Intan Wiratmini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p13

Abstract

Indonesian local chicken is one kind of germplasm that has been utilized by the community for various needs, such as consumption, ceremonial, collection, and cockfighting. To maintain its sustainability, an inventory of genetic diversity can be made using molecular markers. This study aimed to determine the genetic diversity and relationship between four Indonesian local chickens: ayam kampung, ayam ketawa, ayam kate lokal, and ayam bekisar kangean, based on RAPD marker. DNA was extracted from blood samples of 12 local chickens consisting of 3 ayam kampung, 3 ayam ketawa, 3 ayam kate lokal, and 3 ayam bekisar kangean using Genetic DNA Mini Kit (Tissue) Geneaid. The PCR-RAPD analysis was performed using 6 primers, OPA 04, OPB 08, OPB 12, OPC 16, OPH 01, and OPH 03. The product of PCR-RAPD was electrophoresed using 1,5% agarose in 1x TAE buffer. The results showed that the similarity value of 12 local chickens using RAPD ranged from 0% to 100%. The relationship between 12 local chickens using RAPD marker indicated that the chickens in the same morphology were not clustered in the same group. This suggests that the same group of chickens phenotypically does not necessarily have the same genotype. Keywords: Local Chicken, PCR-RAPD
Kadar Hormon Estrogen Dan Ketebalan Endometrium Tikus (Rattus norvegicus) Betina Ovariektomi Setelah Pemberian Ekstrak Daun Lamtoro (Leucaena leucocephala Lam. De Wit) Maria Antonia Margaretha Fernandez; Ngurah Intan Wiratmini; Ni Made Rai Suarni
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p15

Abstract

Lamtoro (Leucaena leucocephala Lam. De Wit) merupakan tanaman yang digunakan sebagai obat dan pakan ternak. Lamtoro mengandung zat aktif dari golongan senyawa streroid, alkaloid, flavonoid, polifenol dan tannin. Zat aktif yang terkandung pada lamtoro bersifat fitoestrogen karena mengandung golongan senyawa steroid, alkaloid dan flavonoid. Tujuan penelitian ini untuk mengetahui pengaruh pemberian ekstrak daun lamtoro terhadap kadar hormon estrogen dan ketebalan endometrium tikus (Rattus norvegicus) betina yang telah diovariektomi. Penelitian ini menggunakan Rancangan Acak Lengkap dengan empat perlakuan yaitu K- (kontrol negatif diberi minyak jagung), K+ (kontrol positif diberi estrogen sintetik 2 mg), P1dan P2 diberi diberi ekstrak daun lamtoro sebanyak 200 mg/kg BB dan 250 mg/kg BB). Masing masing perlakuan terdiri dari 6 ulangan sehingga digunakan 24 ekor tikus betina. Ekstrak daun lamtoro diberikan secara oral sebanyak 2 ml/ekor/hari selama 15 hari. Data yang diperoleh dianalisis dengan One Way ANOVA dan dilanjutkan dengan uji Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan pemberian ekstrak daun Lamtoro (Leucaena leucocephala Lam. De Wit) dosis 250 mg/kg BB dapat meningkatkan kadar hormon estrogen serum darah dan ketebalan endometrium tikus betina yang diovariektomi. Kata kunci: Leucaena, Ovariektomi, Estrogen, Endometrium, Tikus
Histopatologi serta Aktivitas Hati Kelinci Lokal (Lepus sp.) yang Diberi Ransum Tepung Daun Kaliandra (Calliandra calothyrsus Meissn.) dan Kulit Nanas (Ananas comosus L.) I Gede Wiranatha; Iriani Setyawati; Ngurah Intan Wiratmini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p07

Abstract

The aimed of this study was to determine the effect of feed containing various concentrations of Calliandra calothyrsus Meissn. leaf meal mixed with pineapple peels and concentrate feed on histopathology and the activity of liver of local rabbit (Lepus sp). This study used a Post-Test Only Control Group Design, using 24 local male rabbits divided into 4 groups of feed treatment namely P0 = concentrate feed as control; P1 = mixture of 15% of calliandra leaf meal (CLM), 30% of pineapple peels (PP) and 55% of commercial feed (CF); P2 = 30% of CLM, 30% of PP and 40% of CF; and P3 = 45% of CLM, 30% of PP and 25% of CF). The treatments were given for 60 days and used 6 replicates for each group. The data were analyzed statistically by SPSS program version 22. The research results showed the increase of calliandra leaf meal levels in concentrated feed which were added 30% of pineapple peels still caused liver damage namely fatty degeneration, necrosis and congestion. It increased the ALT level, but did not affect the AST level in local rabbit serum.
UJI AKTIVITAS EKSTRAK BIJI KAPUK RANDU (Ceiba pentandra Gaertn.) TERHADAP PERILAKU KAWIN TIKUS (Rattus norvegicus) JANTAN Dessy Sukma Wirastuti; Ngurah Intan Wiratmini; Sang Ketut Sudirga
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p02

Abstract

Kapuk randu (Ceiba pentandra Gaertn.) merupakan salah satu tanaman yang memiliki banyak manfaat untuk masyarakat. Tanaman ini dimanfaatkan sebagai penahan tanah dari erosi, tanaman peneduh, bahan pengisi kasur dan pakan ternak. Saat ini biji kapuk randu banyak dikembangkan sebagai bahan obat kontrasepsi herbal. Tujuan penelitian ini adalah untuk mengetahui aktivitas ekstrak biji kapuk randu (Ceiba pentandra Gaertn.) terhadap perilaku kawin tikus (Rattus norvegicus) jantan. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 4 kelompok perlakuan dan 8 ulangan yaitu P0 (aquades dan tween 80), P1 (0,07 mg/kg BB ekstrak biji kapuk randu), P2 (0,12 mg/kg BB ekstrak biji kapuk randu), P3(0,24 mg/kg BB ekstrak biji kapuk randu). Pemberian perlakuan ekstrak biji kapuk dilakukan selama 33 hari dengan pengamatan perilaku kawin dilakukan 3 hari sekali. Parameter yang diamati untuk menilai adanya perilaku kawin adalah jumlah kissing vagina, jumlah mount jumlah intromission. Hasil penelitian menunjukkan bahwa ekstrak biji kapuk randu memberikan pengaruh yang nyata terhadap perilaku kawin tikus jantan yang ditunjukkan dengan menurunnya jumlah kissing vagina, jumlah mount dan jumlah intromission tikus jantan kelompok perlakuan.
Gambaran Histologi Lambung Dan Duodenum Mencit (Mus musculus L.) Jantan Yang Diberi Ekstrak Daun Kersen (Muntingia calabura L.) Setelah Diinduksi Monosodium Glutamat (MSG) Ni Wayan Adya Puttry Purna Yogini; Ngurah Intan Wiratmini; Ni Gusti Ayu Manik Ermayanti
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p02

Abstract

The antioxidant contained in kersen (Muntingia calabura L.) leaves has been known to be able to prevent animal tissue damages. Monosodium glutamate (MSG) is a food additive substance associated with histological damage of duodenum as well as increases secretion of HCl in stomach if consumed in excess amount. This study aimed to investigate the histological features of stomach and duodenum of monosodium glutamate-induced mice (M. musculus) after treated with kersen leaves extract, as well as establishing its optimum dose. This study used a Completely Randomized Design, with 25 male mice assigned to 5 treatment groups: 2 control groups i.e. negative control (K-) given aquades and positive control (K+) given 0.7mg/body weight MSG; and 3 intervention groups which were given kersen leaves extract as much as 0.075 mg/g bw (P1), 0.15 mg/g bw (P2) and 0.3 mg/g bw (P3). Both kersen leaves extract and MSG was administered orally once daily for 28 days. The parameters observed were histological features of stomach features (erosion of villi and inflammatory cells infiltration) and duodenum (erosion of villi, necrosis, and inflammatory cell infiltration). The results showed that all parameters observed in the aforementioned organs were significantly different (P<0.05) in intervention groups (P1, P2, dan P3) compared with positive control (K+). It is concluded that administration of kersen leaves extract improved the damages of stomach, duodenum, and liver of MSG-induced mice, and was best achieved at the dose of 0.3 mg/g bw.
Uji Efektivitas Neuroprotektif Ekstrak Daun Pepaya (Carica papaya L.) Terhadap Sel Piramidal Di Hipokampus Dan Korteks Serebri Mencit (Mus musculus L.) Yang Diinduksi Trimetiltin Siti Masnunah; Ngurah Intan Wiratmini; Ni Made Rai Suarni
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p05

Abstract

Penelitian ini bertujuan untuk mengetahui efek neuroprotektif ekstrak daun pepaya (Carica papaya L.) terhadap sel piramidal di hipokampus dan korteks serebri mencit (Mus musculus L.) yang diinduksi Trimetiltin serta untuk mengetahui dosis optimum ekstrak daun pepaya yang bersifat neuroprotektif. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan lima perlakuan yang terdiri dari K+ (hanya diinduksi TMT dosis 0,6 mg/kgBB), K- (hanya diberikan Na-CMC 0,5%), P1 (diinduksi TMT dan diberi ekstrak daun pepaya dosis 500 mg/kgBB), P2 (diinduksi TMT dan diberi ekstrak daun pepaya dosis 1000 mg/kgBB), P3 (diinduksi TMT dan diberi ekstrak daun pepaya dosis 2000 mg/kgBB). Parameter yang diamati adalah nekrosis sel piramidal dan persentase kerusakan sel piramidal pada hipokampus dan korteks serebri. Hasil penelitian menunjukkan terjadi penurunan persentase kerusakan sel piramidal di hipokampus pada P1 sebesar 26,46%, P2 sebesar 28,91%, dan P3 sebesar 26,67%. Persentase kerusakan sel piramidal di korteks serebri mengalami penurunan pada P1 sebesar 9,62%, P2 sebesar 11,14%, dan P3 sebesar 14,88%. Dosis optimum ekstrak daun pepaya yang bersifat neuroprotektif adalah dosis 2000 mg/kgBB (P3). Kata kunci: daun pepaya, TMT, neuroprotektif, nekrosis, hipokampus
Co-Authors A.A.S. A. Sukmaningsih AA Istri Mas Padmiswari Anak Agung Gde Raka Dalem Apriliani, Sinta Arnenda, Gussasta Levi Bram Setyadji Dessy Sukma Wirastuti Elfira Dzikri Ashfahani G.A.A Rina Febrina Gussasta Levi Arnenda I Gede Wiranatha I GUSTI AYU MANIK ERMAYANTI I Made Sara Wijana I Made Sara Wijana I Putu Ari Wijana Dipa I Wayan Andi Yoga Kurniawan I Wayan Kasa I.M.A.S. Wijaya Ida Ayu Nopiari Ida Ayu Ratih Purnama Dewi Inna Narayani IRIANI SETYAWATI Jaqueline Sudiman Job Nico Subagio Joko Raharjo, Sentot Joko Wiryatno Luh Putu Wrasiati MADE PHARMAWATI Maria Antonia Margaretha Ferandez Maria Antonia Margaretha Fernandez Mery Wintari Ni Desak Made Aprilia Dewi Ni Komang Tia Pramesti Ni Luh Eka Setiasih Ni Luh Suriani Ni Made Rai Suarni Ni Putu Adriani Astiti Ni Putu Ayu Dewi Wijayanti Ni Putu Sri Risa Dewi Ni Wayan Adya Puttry Purna Yogini Ni Wayan Sudatri Putu Titah Kawitri Resen Salsabillah Rahma SANG KETUT SUDIRGA shanon lestari Siti Masnunah Sri Sulastri Sukma Sushanti Wahyu Puji Lestari WAHYU WIDOWATI