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All Journal Buletin Peternakan INTEGRALISTIK JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Komputer Terapan JURNAL PENDIDIKAN TAMBUSAI Indonesian Journal of Community Services Al Huquq : Journal of Indonesian Islamic Economic Law SINTA Journal (Science, Technology, and Agricultural) Proceeding Applied Business and Engineering Conference Inovasi Jurnal Pengabdian Masyarakat (IJPM) Jurnal Pengabdian Masyarakat dan Riset Pendidikan Marhalado Economic Development Progress
Ananda, Ananda
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The Quality of Frozen Buffalo Sperm Following Sexing using Bovine Serum Albumin (BSA) Column and Swim-Up (SU) Methods Masrizal, Masrizal; Afriani, Tinda; Wahyudi, Dwiki; Saharani, Savira; Ananda, Ananda
Buletin Peternakan Vol 48, No 1 (2024): BULETIN PETERNAKAN VOL. 48 (1) FEBRUARY 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i1.89394

Abstract

This study assesses the impact of two sperm sexing techniques, the Bovine Serum Albumin (BSA) column method, and the swim-up (SU) method, on frozen buffalo spermatozoa quality.  A total of 50 straws of frozen buffalo semen were used in this study. Spermatozoa quality was evaluated before (post thawing) and after the spermatozoa sexing process. Spermatozoa trapped in BSA upper fraction, BSA lower fraction, SU upper fraction, and SU lower fraction were separately evaluated. The parameters measured consisted of  spermatozoa motility, viability, intact plasma membrane, intact acrosome cap, and spermatozoa DNA integrity. The results indicated that the quality of post-thawing buffalo spermatozoa remained relatively high, with motility at 41%, viability at 64.48%, intact plasma membrane at 55.42%, intact acrosome cap at 47.12%, and sperm DNA integrity at 74.94%. However, the use of the BSA column method significantly (p<0.05) decreased spermatozoa quality in both the upper and lower fractions, resulting in motility levels of 34% and 32%, viability rates of 49.36% and 44.71%, intact plasma membrane percentages of 44.78% and 37.13%, intact acrosome cap figures of 37.58% and 33.27%, and sperm DNA integrity levels of 74.76% and 72.45%, respectively. In contrast, the application of the SU method proved effective in preserving post-thawing spermatozoa quality, yielding motility rates of 42% and 41%, viability levels of 63.62% and 62.78%, intact plasma membrane percentages of 54.42% and 54.74%, intact acrosome cap figures of 46.94% and 45.74%, and sperm DNA integrity values of 70.57% and 70.01%, respectively. In summary, after freezing, the SU method excel the BSA column method in maintaining the quality of buffalo spermatozoa post-thawing.
Sperm Kinematics of Pesisir Bull Thawed at Different Temperatures and Times Hendri, Hendri; Jaswandi, Jaswandi; Indriastuti, Rhesti; Ananda, Ananda
Buletin Peternakan Vol 48, No 4 (2024): BULETIN PETERNAKAN VOL. 48 (4) NOVEMBER 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i4.96459

Abstract

Pesisir bulls from West Sumatra, Indonesia, have the potential to be valuable meat producers due to their adaptability and unique characteristics, but reproductive challenges necessitate the use of artificial insemination (AI) technology. The success of AI relies on the quality of frozen semen, which is influenced by thawing conditions, making the evaluation of sperm kinematics through Computer-Assisted Sperm Analysis (CASA) crucial for a comprehensive assessment of sperm function. This research evaluated the kinematics of frozen semen spermatozoa from Pesisir bulls. Frozen semen samples (n = 20) were thawed at 37°C for 10, 20, and 30 s and at 25°C for 30, 45, and 60 s. Kinematic parameters were observed using a Computer-Assisted Sperm Analyzer (CASA), including total motility (M), progressive motility (PM), the velocity of the average path (VAP) (?m/sec), the velocity of curvilinear (VCL) (?m/sec), the velocity of straight line (VSL) (?m/sec), straightness (STR) (%), linearity (LIN) (%), wobble (WOB) (%), amplitude of lateral head displacement (ALH) (?m), and beat/cross-frequency (BCF) (Hz). Thawed frozen semen at a temperature of 37°C for 20 s significantly (p<0.05) increased M, PM, and ALH. Furthermore, thawed frozen semen at 37°C for all durations and at 25°C for 60 s showed better quality for VAP, VCL, and VSL (p<0.05). Meanwhile, thawed frozen semen at 25°C increased STR, LIN, and WOB. However, thawing temperature and duration had no significant effect on BCF. Correlation analysis conducted on semen thawed at a temperature of 37°C for 20 s found that VAP was correlated with VCL, VSL, and ALH, while semen thawed at a temperature of 25°C for 60 s found that VAP was correlated with VCL, VSL, STR, LIN, and ALH. Most kinematic parameters were significantly better in the thawing treatment at 37°C compared to 25°C, suggesting careful consideration 
Co-Authors Ade Sukma Alif Budiman, M. Puja Alif Budiman Anggraini Anggraini Anisa Anisa Annisa Putri Arfah, Yenni Asri asri Astrid, Astrid Aulia, Surya Azhar Amrullah Hafizh Cici, Cici Eka Putra Febriana Febriana, Febriana Gina Lestari, Gina Gustina DM, Venny Hamdini, Hamdini Hasanah, Zahratul Hendri Hendri Idrus M. Said Ikhlas Pratama Sandy Indri Juliyarsi Indriastuti, Rhesti Jaswandi . Kaiin, Ekayanti Mulyawati Keristin, Usnia Wati Magnalia, Mutiara Maman Rachman Martien Herna Susanti Masyita Masyita Melia, Sri Muhammad Farid Navlia, Rusdiana Novia, Devi Nurfadillah Nurfadillah Nurfaizah Nurfaizah, Nurfaizah Pane, Danang Putra Rahmadhani Fitri Ramadani, Magfirah Indah Rifki, Adam Muhammad Risnal Diansyah, Risnal Rizki Dwi Setiawan Rosniar Rosniar, Rosniar Saharani, Savira Salsabila, Andhini Aulia Sapar Sapar Saprianto, Saprianto Sari, Deta Yosfa Suarghana, Yayan Vebriyanti, Ely Wahyudi, Dwiki