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Potensi Antibakteri Ekstrak Spons Laut Koleksi Perairan Grand Watu Dodol Banyuwangi Dian Sari Maisaroh; Nor Sa'adah; Yahya Abdillah Al Hanif
Jurnal Perikanan dan Kelautan Vol 13, No 2 (2023)
Publisher : JURNAL PERIKANAN DAN KELAUTAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jpk.v13i2.22352

Abstract

This study aims to the antibacterial activity of sea sponge extract from the waters of Grand Watu Dodol Banyuwangi against the pathogenic bacteria Staphylococcus aureus, Vibrio parahaemolyticus, Escherichia coli and Escherichia coli MDR (Multi Drug Resistant). This study uses an experimental laboratory method. The samples found were 3 sponges and macerated using methanol solvent with 3 repetitions. The results of the sponge extract paste were tested by disc diffusion method against pathogenic bacteria S. aureus, V. parahaemolyticus, E. coli and E. coli MDR. Antibacterial test using a concentration of 10 mg/ml with 2 repetitions. The extraction results obtained from the four samples only 3 that can produce bioactive compound extracts. Antibacterial activity against pathogenic bacteria S. aureus produced the largest inhibition zone obtained by GWD B with an average of 8.89 mm. Pathogen E.coli, the largest inhibition zone obtained by GWD B with an average of 8.90 mm. Pathogen V. parahaemolyticus the largest inhibition zone obtained by GWD A produced an inhibition zone with an average of 7.30 mm. Pathogen E.coli MDR produced the largest inhibition zone obtained by GWD A with the largest average inhibition zone of 7.26 mm.
Struktur Komunitas Plankton di Pantai Wisata Kampung Kerapu Situbondo Era Fitri Awwalia; Misbakhul Munir; Dian Sari Maisaroh
Juvenil Vol 6, No 1: Februari (2025)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v6i1.27321

Abstract

ABSTRAKPerairan Pantai Wisata Kampung Kerapu dipengaruhi aktivitas manusia, seperti pembuangan limbah dari budidaya ikan kerapu, pemukiman, dan pariwisata dapat mempengaruhi kualitas perairan dan keberadaan organisme. Contohnya, fitoplankton yang berfungsi sebagai produsen primer dan zooplankton sebagai konsumen pertama. Penelitian ini bertujuan untuk mengukur nilai parameter lingkungan perairan, mengetahui struktur komunitas plankton dan menganalisis hubungan struktur komunitas plankton dengan parameter lingkungan di perairan Pantai Wisata Kampung Kerapu Situbondo. Penelitian ini dilaksanakan pada bulan Maret 2024 di Pantai Wisata Kampung Kerapu Situbondo. Penelitian ini menggunakan metode deskriptif dan analisis data menggunakan Priciple Component Analysis (PCA). Metode penentuan stasiun menggunakan purposive sampling. Hasil dari penelitian ini menunjukkan bahwa kualitas air dengan parameter suhu, kecerahan, pH, DO, salinitas, fosfat dan BOD masih sesuai dalam baku mutu KEPMEN LH no.51 tahun 2004 untuk biota laut kecuali nitrat. Struktur komunitas plankton meliputi kelimpahan (N) fitoplankton dan zooplankton sebesar 2.213 – 3.821 ind/ml dan 518 – 883 ind/ml, keanekaragaman (H’) fitoplankton dan zooplankton sebesar 1,71 – 2,27 dan 1,42 – 2,08, keseragaman (E) fitoplankton dan zooplankton sebesar 0,67 – 0,88 dan 0,59 – 0,87, dominansi (C) fitoplankton dan zooplankton sebesar 0,12 – 0,23 dan 0,14 – 0,29. Hubungan kualitas perairan terhadap struktur komunitas plankton adalah salinitas, BOD, fosfat, pH, dan suhu. Kata Kunci: Struktur Komunitas Plankton, Parameter Perairan, Principle Component Analysis (PCA), SitubondoABSTRACTThe waters of the Kampung Kerapu Tourism Beach affect human activities, such as the disposal of waste from grouper cultivation, settlements, and tourism which can affect the quality of the waters and the existence of organisms. For example, phytoplankton function as primary producers and zooplankton as primary consumers. This research aims to measure the value of aquatic environmental parameters, determine the structure of the plankton community and analyze the relationship between plankton community structure and environmental parameters in the waters of the Kampung Kerapu Tourism Beach, Situbondo. This research was carried out in March 2024 at the Kampung Kerapu Tourism Beach, Situbondo. This research uses descriptive methods and data analysis using Principle Component Analysis (PCA). The method for determining stations uses purposive sampling. The results of this research show that water quality with the parameters of temperature, brightness, pH, DO, salinity, phosphate and BOD still conforms to the quality standards KEPMEN LH no. 51 of 2004 for marine biota except nitrate. The plankton community structure includes abundance (N) of phytoplankton and zooplankton of 2,213 – 3,821 ind/ml and 518 – 883 ind/ml, diversity (H') of phytoplankton and zooplankton of 1.71 – 2.27 and 1.42 – 2.08, uniformity (E) of phytoplankton and zooplankton is 0.67 – 0.88 and 0.59 – 0.87, dominance (C) of phytoplankton and zooplankton is 0.12 – 0.23 and 0.14 – 0.29. The relationship between water quality and plankton community structure is salinity, BOD, phosphate, pH, and temperature.Keywords: Structure Plankton Community, Water Parameters, Principle Component Analysis (PCA), Situbondo
Potensi Antifouling Ekstrak Teripang (Phyllophorus sp.) Terhadap Bakteri Biofilm Di Beton Perairan Jembatan Suramadu Arnanda, Nisya; Maisaroh, Dian Sari; Perdanawati, Rizqi Abdi
Jurnal Kelautan Vol 18, No 2: Agustus (2025)
Publisher : Department of Marine Sciences, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jk.v18i2.27332

Abstract

ABSTRAKPenempelan biota pada permukaan beton diawali dengan tahapan penempelan koloni bakteri dan mikroalga (biofilm). Fenomena ini terjadi pula pada beton di jembatan Suramadu. Biofilm yang nantinya berkembang menjadi makrofouling rentan menimbulkan kerusakan sehingga diperlukan upaya untuk menghambat penempelan biofilm. Penelitian ini bertujuan untuk menganalisa kemampuan antifouling ekstrak teripang (Phyllophorus sp.) terhadap biofilm yang muncul di perairan jembatan Suramadu. Pengambilan sampel biofilm didapatkan dari permukaan rendaman beton di perairan jembatan Suramadu. Tahapan penelitian dimulai dari identifikasi bakteri biofilm secara morfologi, pembuatan ekstrak teripang dan pengujian antifouling dengan uji fitokimia dan uji zona hambat. Uji zona hambat ekstrak teripang (Phyllophorus sp.) dilakukan dengan hasil rendemen sebesar 5,64% dengan 2 variasi ekstrak uji yaitu 100% ekstrak dan 50% ekstrak. Bakteri biofilm diketahui terdiri dari bakteri gram positif kokus, gram positif basil, gram negatif kokus dan tidak ditemukan gram negatif basil. Hasil uji fitokimia sebagai agen antifouling yang dapat menghambat pertumbuhan biofilm antara lain fitokimia alkaloid, flavonoid dan terpernoid. Tingkat hambat ekstrak dengan kategori kuat ditemukan pada variasi ekstrak 100% yaitu 2,34–10,92 mm pada 5 isolat, pada ekstrak 50% zona hambat pada kategori lemah. Hasil ini menunjukkan adanya potensi penggunaan ektrak teripang (Phyllophorus sp.) terhadap biofilm yang muncul di perairan jembatan Suramadu.Kata kunci: Antifouling, Biofilm, Biofouling, Ekstrak Teripang, Jembatan SuramaduABSTRACTBiofouling on the concrete surface begins with the adhesion of bacterial and microalgae colonies (biofilm). This phenomenon also occurs on the concrete of the Suramadu Bridge. Biofilms, which eventually develop into macrofouling, are prone to causing damage, necessitating efforts to inhibit biofilm attachment. This study aims to analyze the antifouling potential of sea cucumber extract (Phyllophorus sp.) against biofilms found in the waters of the Suramadu Bridge. Biofilm samples were obtained from the surface of concrete submerged in the waters around the Suramadu Bridge. The research stages included the morphological identification of biofilm bacteria, preparation of sea cucumber extract, and antifouling testing through phytochemical analysis and inhibition zone tests. The inhibition zone test of the sea cucumber extract (Phyllophorus sp.) yielded a 5.64% extract with two variations tested: 100% extract and 50% extract. The biofilm bacteria were identified as gram-positive coccus, gram-positive bacilli, gram-negative coccus, but none of gram-negative bacilli found. The phytochemical analysis indicated that alkaloids, flavonoids, and terpenoids in the extract served as antifouling agents capable of inhibiting biofilm growth. A strong inhibition was observed in the 100% extract variation by the diameter 2,34 – 10,92 mm for 5 isolats, while the 50% extract showed weak inhibition. These results demonstrate the potential use of sea cucumber extract (Phyllophorus sp.) in controlling biofilm formation in the waters surrounding the Suramadu Bridge..Keyword: Antifouling, Biofilm, Biofouling, Sea Cucumber Extract, Suramadu Bridge
ANALISIS LAJU PERTUMBUHAN PADA BUDIDAYA IKAN NILA SALIN DI KOLAM HDPE IBAP BANGIL, PASURUAN Fadilah, Achmad Akmal; Maisaroh, Dian Sari; irmansyah, Pandu Rahman F; Firmansyah, Ryan Achmad; Saputra, Ayung
Prosiding Seminar Nasional Biologi Vol. 13 (2025)
Publisher : Prosiding Seminar Nasional Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk menganalisis laju pertumbuhan ikan nila salin (Oreochromis niloticus) yang dibudidayakan di kolam HDPE pada Instalasi Budidaya Air Payau (IBAP) Bangil, Pasuruan. Ikan nila salin adalah ikan nila yang mampu beradaptasi pada kondisi perairan payau. Penelitian dilakukan selama Februari hingga April 2025 menggunakan metode eksperimen dengan pendekatan kuantitatif. Parameter yang diamati meliputi kualitas air (suhu, pH, DO, dan salinitas), manajemen pakan, Average Body Weight (ABW), serta Average Daily Growth (ADG). Hasil pengamatan menunjukkan kualitas air berada pada kisaran optimal bagi pertumbuhan ikan nila salin, dengan suhu 27,5–31,6°C, pH 7,3–8,4, DO 4,73–8,23 mg/L, dan salinitas 10–11 ppt. Pertumbuhan ikan berlangsung stabil dengan peningkatan bobot rata-rata dari 15 g (DOC 20) menjadi 75 g (DOC 70). Nilai ADG menunjukkan fluktuasi antara 0,5–2 g/hari yang dipengaruhi oleh pengaturan pakan untuk menghindari overfeeding, sementara sistem semi-intensif tetap mendukung pertumbuhan melalui pakan alami. Secara keseluruhan, budidaya ikan nila salin di kolam HDPE menunjukkan performa pertumbuhan yang baik dan dapat menjadi alternatif budidaya berkelanjutan di wilayah pesisir.
Pengaruh Faktor Oseanografi dan Suspensi Sedimen terhadap Pertumbuhan dan Mortalitas Karang Transplantasi (Acropora spp.) di Paiton Probolinggo Supriyadi; Asri Sawiji; Maisaroh, Dian Sari
Journal of Marine Resources and Coastal Management Vol. 1 No. 1 (2020)
Publisher : UIN Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/mrcm.v1i1.749

Abstract

ABSTRAK Transplantasi karang merupakan salah satu upaya dalam memulihkan kondisi terumbu karang yang semakin mengalami degradasi. Transplantasi dapat dilakukan menggunakan berbagai media, salah satunya adalah dengan kanstin FABA dari limbah padat batu bara fly ash bottom ash yang dihasilkan oleh PLTU Paiton. Fragmen karang batu Acropora spp. ditransplantasikan pada dua kedalaman berbeda. Metode penentuan titik pengamatan menggunakan purposive sampling dengan pertimbangan jarak lokasi transplantasi ke lokasi bibit (± 7 meter). Penelitian ini menggunakan metode eksperimental dengan membandingkan dua kondisi penelitian, yaitu transplantasi pada kedalaman 6 m dan 8 m dengan 10 fragmen Acropora formosa dan 10 fragmen Acropora intermedia di setiap kedalaman. Pengamatan dilaksanakan setiap dua minggu meliputi faktor oseanografi dan laju pertumbuhan karang. Hasil penelitian menunjukan bahwa faktor oseanografi di lapangan menunjukan nilai yang sesuai untuk mendukung pertumbuhan karang. Laju sedimentasi tertinggi terdapat di kedalaman 8 m yaitu sebesar 25,57 mg/cm2/hari yang termasuk dalam kategori sedang dan mempengaruhi kelimpahan karang. Pengaruh ini dibuktikan adanya kematian Acropora spp. menjadi death coral algae dengan indeks mortalitas sebesar 0,1. Laju pertumbuhan karang transplantasi pada kedalaman 6 m sebesar 2,2 mm/minggu untuk Acropora formosa dan sebesar 3,1 mm/minggu untuk Acropora intermedia, sedangkan kedalaman 8 m Acropora formosa memiliki laju pertumbuhan sebesar 2,0 mm/minggu dan sebesar 2,4 mm/minggu untuk Acropora intermedia. Faktor oseanografi dengan pengaruh dominan terhadap laju pertumbuhan yaitu salinitas dengan koefisien korelasi 0.853 (hubungan searah) dan kedalaman memiliki pengaruh dengan hubungan terbalik yaitu -0.244. Laju sedimentasi memiliki koefisien korelasi sebesar 0.8 terhadap pertumbuhan dan 0.54 terhadap mortalitas. Kata Kunci: Transplantasi karang batu, kanstin FABA, indeks mortalitas, PCA.
Study of ectoparasite prevalence and intensity on mud crab (Scylla serrata) in mangrove area of Wonorejo, Surabaya Purna, Selobing; Munir, Misbakhul; Maisaroh, Dian Sari
Journal of Marine Resources and Coastal Management Vol. 2 No. 1 (2021)
Publisher : UIN Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/mrcm.v2i1.1121

Abstract

This study aims to know various types, prevalence, and intensity of ectoparasite on mud crab (Scylla serrata) in mangrove area Wonorejo, Surabaya.  This study used survey method with random sampling to collect the data.  This study was conducted in July-December 2020. Ectoparasite in this study collected by smear method.  In-situ water quality measurement were applied in this study.  Mud crab in this study was obtained from local fisherman that consist of 30 crabs.  There are 3 types of ectoparasite was infected mud crab there are Octolasmis sp. (208 ind), Zoothamnium sp. (93 ind), and Epistylis sp. (116 ind).  Those ectoparasite was found in mud crabs gills.  The highest prevalence was Octolasmis sp. (70%), Epistylis sp. (30%), and Zoothamnium sp. (23%).  The highest intensity was Zoothamnium sp (13.29 ind/crab), Epistylis sp. (12.89 ind/crab), and Octolasmis sp. (9.90 ind/crab).  Based on Ministerial Decree of Ministry Environment and Forestry No. 1 of 2004 water quality in Wonorejo mangrove area was still optimal for life cycle of mud crab with average of temperature is 31.20°C, Dissolved Oxygen 5.53 mg/l, salinity 11 ppt, and pH 7.40.
Chitosan from milkfish (Chanos chanos) scales and tiger shrimp (Panaeus monodon) shells wastes as corrosion inhibitor on ASTM A36 steel Silvia, Chandra; Munir, Misbakhul; Maisaroh, Dian Sari
Journal of Marine Resources and Coastal Management Vol. 2 No. 1 (2021)
Publisher : UIN Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/mrcm.v2i1.1122

Abstract

Chitosan synthesized from milkfish (Chanos chanos) scales and tiger shrimp (Panaeus monodon) shells were used as corrosion inhibitors on ASTM A36 steel. Milkfish scales and tiger shrimp shells wastes is extracted into chitosan through deproteination, demineralization and deacetylation process. The yield of milkfish scales and tiger shrimp chitosan was 31,81% and 67,16%, respectively. Corrosion rate testing using weight loss method then calculates inhibition efficiency of each inhibitor. The immersion test of specimens was carried out for four weeks with weight measurements every week. The concentrations used were 0 ppm as negative control; 60 ppm, 500 ppm and 1000 ppm for chitosan inhibitor; and 1000 ppm sodium nitrite as positive control. Inhibition efficiency values ​​produced by milkfish scales chitosan, starting from the first to the fourth week immersion, for concentration of 60 ppm were 43.9%, 46.8%, 48.9%, and 40.8%; for concentration of 500 ppm were 43.6%, 43.3%, 37.8%, and 17.8%; and for concentration of 1000 ppm were 37.5%, 44.9%, 39%, and 21.8%; while for tiger shrimp shells chitosan for concentration of 60 ppm were 37.6%, 51%, 34.6%, and 28.5%; for concentration of 500 ppm were 57.7%, 38.2%, 37.7%, and 19.6%; and for concentration of 1000 ppm were 48.6%, 41.2%, 37%, and 21.3%. Comparison of inhibition efficiency between chitosan from milkfish scales and tiger shrimp shells based on the statistical analysis One Way ANOVA resulted in a sig >0.05 which indicates a similarity in the efficiency value of chitosan from milkfish scales and tiger shrimp shells.
Effect of local sea cucumber (Phyllophorus sp.) methanol extract as natural antifouling against macrofouler (Nerita sp.) Hasanah, Himatul; Maisaroh, Dian Sari; Perdanawati, Rizqi Abdi
Journal of Marine Resources and Coastal Management Vol. 2 No. 1 (2021)
Publisher : UIN Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/mrcm.v2i1.1128

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This study aims to find out the activities of local sea cucumber methanol extract (Phyllophorus sp.) as a natural antifouling against macrofouler Nerita sp. This study uses experimental methods laboratory. Laboratory-scale data collection is carried out by observation of biota paste activities fouling on the aquarium (antifouling rate) as well as its regaining rate. This research uses research design complete random design (RAL) with three repetitions. Local sea cucumber methanol extract (Phyllophorus sp.) contains bioactive compounds such as saponins, alkaloids, tannins, steroids and phenolics. Methanol Extract local sea cucumber (Phyllophorus sp.) affects the antifouling rate by reducing the Nerita sp. macrofouler paste rate with increasing concentration. It's the same thing,occurs where the increasing concentration of local sea cucumber methanol extract (Phyllophorus sp.) provides a significant decrease in Nerita sp. macrofouler regaining capability. The results lethal concentration 50 or LC50 obtained a value of 185.18. This means the concentration of the extract sea cucumber (Phyllophorus sp.) is deadly 50% of all biota test at a concentration of 185.18 mg/ml. Based on antifouling rate analysis and regaining rate it is known that the concentration of the most effective that can be used as an antifouling is a concentration of 75 mg/ml. This is because a concentration of 75 mg/ml of local sea cucumber methanol extract (Phyllophorus sp.) can already inhibit paste macrofouler Nerita sp. The concentration of 75 mg/ml also has a large regaining rate of 85%.
Mangrove (Bruguiera gymnorrhiza) leaves potential as colouring agent on cotton, rayon, and sateen fabrics Anggryani, Cucuk Trisnawati; Munir, Misbakhul; Maisaroh, Dian Sari
Journal of Marine Resources and Coastal Management Vol. 2 No. 1 (2021)
Publisher : UIN Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/mrcm.v2i1.1146

Abstract

This study aims to find the value of the fastness test of the natural dye of mangrove leaves (Bruguiera gymnorrhiza). This research was conducted in June-November 2020. This research used descriptive and experimental methods. The samples used were cotton, rayon, and sateen fabrics. The experimental results showed the addition of fixator tunjung (FeSO4) capable of binding dye mangrove leaves Bruguiera gymnorrhiza that give color to the fabric fibers. The result of the coloring is brownish yellow. The results of the color fastness value in hot water are influenced by the difference in ΔE (colorfastness), cotton fabrics have a range of values ​​between the medium category (5.1-5.94), the medium-large category rayon cloth (5.09-9.06 ), medium category sateen (3.65-4.35). The results of the color fastness value in cold water are influenced by the difference in ΔE (colorfastness), cotton fabrics have a range of values ​​between the small and medium categories (1.96-3.68), rayon fabrics in the small category (2.83-3.9 ), the small category of sateen fabrics (1.54-2.76).
Stingray (Neotrygon orientalis) population structure based on catch data in Nusantara Fishery Port (Pelabuhan Perikanan Nusantara/PPN) Brondong, Lamongan Sukmawati, Mayang; Setiawan, Fajar; Maisaroh, Dian Sari
Journal of Marine Resources and Coastal Management Vol. 3 No. 1 (2022)
Publisher : UIN Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/mrcm.v3i01.1326

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Stingray is a type of fish included in the Elasmobranchii class that has high economic potential. This study aims to determine the sex ratio, growth rate, length-weight relationship, condition factor, mortality and exploitation rate of stingray (Neotrygon orientalis) in PPN Brondong, Lamongan. Samples N. orientalis collected every week starting from October 1, 2019 until January 2021 and weighed in total body weight (gram) and measured in total body length (cm) then sex was identified based on macroscopic characteristics of gonads. The results showed that the sex ratio of N. orientalis was 1.4 : 1. Length growth value (K) of male and female N. orientalis are 0.0285 and 0.0233, respectively, which show a slow growth time to reach the asymptotic length (K≤ 0.5). Based on the analysis of the relationship between length and weight, the results show that b value are 1.9394 and 2.2023 in male and female N. orientalis, respectively, which means length growth rate faster than weight growth (b< 3, negative allometric). N. orientalis are included in the category of flat (thin) fish with condition factor values ranging from 0.2462-3.9227. The total, natural, and fishing mortality rate were 0.395, 0.177, and 0.218, respectively. The exploitation rate is 0.551 where the value has exceeded the optimum value 0.5, which means that N. orientalis landed at the PPN Brondong, Lamongan has indicated overfishing caused by excessive fishing activity.