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All Journal AL KAUNIYAH Teknosains: Media Informasi Sains dan Teknologi Pelita Perkebunan Jurnal Penelitian Sains BIOEDUKASI Bioma : Jurnal Biologi Indonesia BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains AL-HAYAT: Journal of Biology and Applied Biology Jurnal Sains dan Edukasi Sains Indonesian Journal of Life Sciences InSTEM Jurnal Malikussaleh Mengabdi Life Science and Biotechnology
Su'udi, Mukhamad
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Journal : BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains

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Metode Direct PCR yang Cepat dan Hemat Biaya untuk DNA Barcoding Anggrek Obat Dendrobium crumenatum Sw. Afidah, Yusfi; Su'udi, Mukhamad
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i2.12893

Abstract

This study aims to develop a rapid and cost-effective Direct PCR method for amplifying the rbcL gene without requiring DNA isolation, particularly for Dendrobium crumenatum Sw., an orchid species known for its traditional medicinal uses. The method involved soaking fresh leaves of D. crumenatum in TE buffer, followed by incubation at 55ºC. The incubation product was Directly used as a PCR template. The results showed a clear DNA band of 600 bp, corresponding to the target rbcL gene. BLAST analysis confirmed that the DNA sequence shared 84-85% identity with other species in GenBank. In conclusion, the Direct PCR method proved to be effective for DNA barcoding applications, offering advantages in speed and cost-efficiency over conventional DNA isolation methods. Keywords: Medicinal Orchids, Dendrobium crumenatum Sw., Direct PCR, DNA Barcoding
Co-Authors Afidah, Yusfi Aisiyya Qutlana Munawaroh, Putri Amero, Movhalky Argeswara Delta Cancera Ania Ningsih, Misbahul Ardiyansah, Muhammad Ardiyanti, Kurnia Dwi Arum, Asyifa Yasmin Cahyono, Haris DWI INDAHNING ROHMAH Dwi Setyati Dwinianti, Edia Dwinianti, Edia Fitri Fitri, Edia Fitri, Nurfajri Eka Fuad Bahrul Ulum Gunawan, Gilbert Geraldo Hasanah, Lailiyah Maulidatul Ikrimah, Sindhi Wahidatul Iqbaliah, Agil Faiqotul Khoirunnisa, Gita Ayu Munawaroh, Putri Aisiyya Qutlana Mustafidah, Qori'atul Mustafidah, Qori’atul Noviyani, Anis Perwitasari, Dian Al Ghifari Puspito, Agung Nugroho Ramadany, Zakiyah Ravitamala, El Shania Rizqoni, Moh Ikmal Alfi Rizqoni, Moh. Ikmal Alfi Rohmah, Isfina Nurfaidatur Rosida, Waki'atil Rosida, Wakiatil Sattya Arimurti Septianasari, Miatin Alvin Siti Aisyah Siti Rohimah Sulistyani Pancaningtyas Sururin, Fahma Wardah Utami, Diah Ayu Wibowo, Oktavia Putri Wulandari, Wardza Izza Ziyaroh, Putry Lazimatu