Article Per Year (5 Year)
p-Index From 2020 - 2025
3.041
P-Index
This Author published in this journals
All Journal AL KAUNIYAH Teknosains: Media Informasi Sains dan Teknologi Pelita Perkebunan Jurnal Penelitian Sains BIOEDUKASI Bioma : Jurnal Biologi Indonesia BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains AL-HAYAT: Journal of Biology and Applied Biology Jurnal Sains dan Edukasi Sains Indonesian Journal of Life Sciences InSTEM Jurnal Malikussaleh Mengabdi Life Science and Biotechnology
Su'udi, Mukhamad
Unknown Affiliation
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Education Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Other

Published : 23 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search
Journal : Life Science and Biotechnology

 1 
The matK Mini-barcode as a Potential Molecular Identification Tool for Medicinal Orchids Su'udi, Mukhamad; Setyati, Dwi; Khoirunnisa, Gita Ayu; Afidah, Yusfi; Ulum, Fuad Bahrul
Life Science and Biotechnology Vol. 1 No. 1 (2023): May
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i1.39851

Abstract

matK is one of the universal loci which is commonly used as a barcode in the molecular identification of plants. Up to now, the problem of matK as a single locus is the use of the entire gene region as barcodes. The long amplicon size of matK raises several problems in DNA barcoding. The aim of this study is to uncover a small region of the matK gene as a potential mini-barcode region for alternative sites to get more efficient and effective results. In silico studies performed include the collection of matK sequence of medicinal orchid from GenBank, the amplicon size from matK primer sets that are commonly used in the Orchidaceae family, and PCR analysis. Data analysis was done by comparing the results of PCR amplification from several primer sets: matK 390F-1326R, 743F-R2, 712F-1154R and 712F-1216R. The results show that all primer pairs are able to amplify the DNA template isolated from Dendrobium purpureum. However, the shorter DNA band obtained from matK 743F-R2, 712F-1154R and 712F-1216R primers sets were visualized more thick than the longer amplicon band produced from matK primer 390F-1326R. This indicates that short matK sequence for DNA barcoding (mini-barcode) is more efficient in amplifying DNA templates compared to generally applied barcoding using full length sequences.
Propagation of Dendrobium crumenatum Sw. through In Vitro Seed Culture Using Fertilizer-Enriched and Organic Complex Media Su'udi, Mukhamad; Ziyaroh, Putry Lazimatu
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.53562

Abstract

Dendrobium crumenatum Sw. is one of the epiphytic orchids with high ecological and economic value. This study aims to examine the effect of media containing organic compounds enriched with fertilizers for in vitro propagation of D. crumenatum seeds. This culture medium contains organic materials such as potatoes, bananas, coconut water, charcoal, sucrose, and foliar fertilizer. This medium was designed to provide macro, micro, and growth hormone nutrients that support explant growth. The research procedure included sterilization of orchid seeds, explant sowing, and growth observation for about two months. The results showed that orchid seeds experienced optimal growth in media with the addition of coconut water and other organic compounds. The formulation of organic-based media with the addition of coconut water and other supporting compounds is effective in supporting the in vitro growth of orchids, providing opportunities for efficient propagation of orchid species. Explant growth reached 1-2 cm at the 68th day after sowing, with a fresh green colour as an indicator of optimal physiological conditions. The even distribution of shoots indicates that the media provides sufficient space and nutrients without competition between explants. In sum, simple tissue culture media produced from a mixture of organic materials proved reliable function and suited for growing D. crumenatum orchid seeds to produce plantlets, and into further extent it would support conservation efforts. Key words: Conservation, organic materials, pigeon orchid, simple media, tissue culture
Phylogenetic Analysis of Manihot sp. Based on matK and rbcL Markers Rosida, Wakiatil; Ulum, Fuad bahrul; Su'udi, Mukhamad
Life Science and Biotechnology Vol. 3 No. 1 (2025): May 2025
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cassava (Manihot sp.) is a staple crop of major economic and ethnomedicinal importance. Understanding the phylogenetic relationships within the genus Manihot is essential for clarifying its taxonomy and supporting genetic improvement programs. This study aimed to reconstruct the phylogeny of several Manihot species using two chloroplast DNA markers, matK and rbcL. Sequence data were retrieved from the GenBank database, and Ricinus communis was designated as the outgroup. Phylogenetic analyses were performed using the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods, each with 1,000 bootstrap replications to assess branch support. The resulting trees consistently clustered Manihot esculenta within a well-supported monophyletic group, indicating a close genetic relationship with other congeners. Distinct clades corresponding to genetic similarity were also observed among the analyzed species. These findings enhance current knowledge of Manihot systematics, providing molecular evidence that supports its taxonomic classification. Furthermore, the results contribute valuable insights for cassava breeding and conservation efforts through improved understanding of its evolutionary relationships.
Co-Authors Afidah, Yusfi Aisiyya Qutlana Munawaroh, Putri Amero, Movhalky Argeswara Delta Cancera Ania Ningsih, Misbahul Ardiyansah, Muhammad Ardiyanti, Kurnia Dwi Arum, Asyifa Yasmin Cahyono, Haris DWI INDAHNING ROHMAH Dwi Setyati Dwinianti, Edia Dwinianti, Edia Fitri Fitri, Edia Fitri, Nurfajri Eka Fuad Bahrul Ulum Gunawan, Gilbert Geraldo Hasanah, Lailiyah Maulidatul Ikrimah, Sindhi Wahidatul Iqbaliah, Agil Faiqotul Khoirunnisa, Gita Ayu Munawaroh, Putri Aisiyya Qutlana Mustafidah, Qori'atul Mustafidah, Qori’atul Noviyani, Anis Perwitasari, Dian Al Ghifari Puspito, Agung Nugroho Ramadany, Zakiyah Ravitamala, El Shania Rizqoni, Moh Ikmal Alfi Rizqoni, Moh. Ikmal Alfi Rohmah, Isfina Nurfaidatur Rosida, Waki'atil Rosida, Wakiatil Sattya Arimurti Septianasari, Miatin Alvin Siti Aisyah Siti Rohimah Sulistyani Pancaningtyas Sururin, Fahma Wardah Utami, Diah Ayu Wibowo, Oktavia Putri Wulandari, Wardza Izza Ziyaroh, Putry Lazimatu