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All Journal Majalah Kedokteran Bandung Jurnal Permukiman Majalah Kesehatan Pharmamedika Journal of Health and Nutrition Research Makara Journal of Science
Aryenti, Aryenti
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Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Health Professions Medicine & Pharmacology Nursing Public Health

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Journal : Makara Journal of Science

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Effects of Methanolic Jatropha multifida L. Extract in Wound Healing Assessed by the Total Number of PMN Leukocytes and Fibroblasts Juniarti, Juniarti; Aryenti, Aryenti; Yuhernita, Yuhernita; Poerwaningsih, Ernie Ernie
Makara Journal of Science Vol. 16, No. 3
Publisher : UI Scholars Hub

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Abstract

The aim of this study was to evaluate the effects of methanol extract of Jatropha multifida leaves on the wound healing process and to investigate the wound healing activity based on reduced numbers of PMN (polymorpho nuclear) leukocytes and increased numbers of fibroblasts. Methanol extract of dried leaves of J. multifida was used in the wound healing activity studies. The study subjects were 36 white male Sprague Dawlay rats aged 2 months with 150-200 gram body weight. The subjects were divided into 4 groups and experimentally injured: Group I (negative control) underwent injury without subsequent treatment; group II (positive control) received topical treatment with Bethasone-N after injury; group III (solvent control) was treated with 70% methanol; group IV (treatment group) was treated with 10 mg methanol extract of J. multifida Each group consisted of 3 rats, which were decapitated on days 3, 6, and 13 after the start of treatment. Histological preparation was stained with hematoxyline-eosin (HE) and was continuously examined by counting the numbers of PMN leukocytes and fibroblasts as indicators of wound healing on days 3, 6, and 13 of treatment. The study showed lower numbers of PMN leukocytes in subjects treated with the extract of J. multifida as compared to the other groups. The numbers of fibroblasts were significantly higher on days 6 and 13 of treatment. The treatment of injuries with methanol extract of leaves from J. multifida provided better results compared to the other groups in our study.
PMN Leukocytes and Fibroblasts Numbers on Wound Burn Healing on the Skin of White Rat after Administration of Ambonese Plantain Banana Aryenti, Aryenti; Suryadi, Suryadi; Harijadi, Harijadi; Yuhernita, Yuhernita
Makara Journal of Science Vol. 16, No. 1
Publisher : UI Scholars Hub

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A study of ambonese plantain banana (Musa paradisiaca var sapientum Lamb) treatment in burn wound healing on the skin of white rats (Rattus novergicus) has been conducted. The wound healing of burn injuries was evaluated by counting the number of PMN leukocytes and fibroblasts at the 7th, 14th, and 21st days following the treatment. The study showed that the decrease in number of PMN leukocytes of subjects treated with ambonese plantain banana was relatively more significant compared to both negative and positive control (Bioplacenton ®). In contrast, an increasing number of fibroblasts was significantly demonstrated at the 14th and 21st days after treatment. In conclusion, ambonese plantain banana treatment in burn injuries will provide better results compared to both positive and negative controls.
Co-Authors Abdullah, Johari Yap Anggraeni, Fitrijani Arrahmani, Betharie Cendera Batubara, Lilian Damayanti, Ndaru Andri Dewi Nurul, Dewi Dewi, Intan Keumala Endah Purnamasari Hanna Sari Widya Kusuma, Hanna Sari Widya Harijadi Harijadi Juniarti . Juniarti Juniarti Kustiasih, Tuti Maya Genisa MD Yusoff, Bazli Bin Nafik, Said Permata Yuda, Indah Poerwaningsih, Ernie Ernie Sachrowardi, Qomariyah Romadhiyani Setyawati, Lya Meilany Sosiawan, Insan Sri Darwati Sri Utami Suryadi Suryadi Utomo, Herry S WAHYU WIDOWATI Yuhernita, Yuhernita