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All Journal Buletin Peternakan Buletin Udayana Mengabdi Jurnal Veteriner Animal Production : Indonesian Journal of Animal Production Pangkaja: Jurnal Agama Hindu Widya Duta: Jurnal Ilmiah Ilmu Agama dan Ilmu Sosial Budaya
Wayan Sayang Yupardhi
Faculty Of Animal Science, Udayana University, Denpasar, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Social Sciences Veterinary Other

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Gambaran Darah Kambing Gembrong, Kambing Peranakan Etawah, dan Kambing Kacang di Bali (BLOOD ILLUSTRATION OF GEMBRONG GOAT, ETAWAH CROSSBRED GOAT AND KACANG GOAT IN BALI) Wayan Sayang Yupardhi; I Gusti Lanang Oka; Ida Bagus Mantra; I Nyoman Suyasa; I Gede Suranjaya
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

There were three kinds of goat developed in Bali since a long time ago i.e. Gembrong goat, EtawahCrossbred goat, and Kacang goat. Gembrong goat is nearly disappearingrecently due to decrease of itspopulation from year to year. The Gembrong goat has very interesting performance, funny, and this speciesis Balinese native goats. The objective of thisexperiment was to know the blood illustration among thethree speciesof the Balinese native goatwhere in the future it can be used as a guide to develop each ofthem. The experiment was used nine adults goats namely three adult male Gembrong goats, three adultmale Etawah Crossbred goats, and three adult male Kacang Goats respectively. The animal fed dailygreen feed grows around them and additional rice bran. Blood samples of the animals were collected forabout10-15 mL each with venoject, and then was kept in a cooler box and subsequently sent to the ProdiaClinic Laboratory at Denpasar. The results of the experiment showed that blood illustration of the threekinds of goat was not significantly different (P > 0.05) except the triglyseride.
Genetic Relationship Between Gembrong Goat, Kacang Goat and Kacang X Etawah Crossbred (PE) Based on Their Mitochondrial DNA I Gusti Lanang Oka; Wayan SayangYupardhi; Ida Bagus Mantra; Nyoman Suyasa; Anak Agung Sagung Dewi
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Gembrong goat is a specific type of goat which has long hair covers its whole body including its neckand face, originated from eastern part of Bali (Karangasem). A study of this type of goat and its relationshipwith other local goats (Kacang and Kacang x Etawah crossbred) was carried out at Sawe village, Jembrana,Bali.. A number of 12 gembrong goats, 3 kacang goats from Kubu village, Karangasem and 3 Kacang xEtawah crossbred goats from Denpasar were used in this study. Blood samples of all goats were collectedfor mitochondrial DNA (mtDNA) analysis. PCR amplification of D-loop mitochondrial DNA was carriedout by using two primers i.e. CAP-F (5’-CGTGTATGCAAGTACATTAC -3’) and CAP-R (5’-CTGATTAGTCATTAGTCCATC – 3’) . Sequencing of 550 bp (base-pairs) of mitochondrial DNA (productof PCR) only found one polymorphic site at base number 231 with two haplotypes in gembrong goat only,while the other base-pairs were similar between the three goat types (Gembrong, Kacang and Kacang xEtawah crossbred) .The frequency of haplotype 1 was 83.3% and the frequency of haplotype 2 was 16.7%.It was concluded that based on their mitochondrial DNA sequences and “phylogenic analysis”, the threetypes of goat (Gembrong,Kacang and Kacang xEtawah crossbred) had a very close genetic relationship(kinship).
Hematologi dan Kimia Klinik Darah Kambing Peranakan Etawah yang Diberi Pakan Produk Sampingan Pertanian dan Enzim Optizym (HEMATOLOGY AND BLOOD CLINICAL CHEMISTRY OF ETAWAH GOAT CROSSBRED-FED AGRICULTURE BY PRODUCTS SUPPLEMENTED WITH OPTIZYM ENZYME) Wayan Sayang Yupardhi; I Gusti Lanang Oka; Ida Bagus Mantra
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In Indonesia including Bali island until now, a lot of Etawah Goat Crossbred raised by farmers. Inthe island, there was  lots of  agriculture by products available which were not much used yet for animalfeed. There is an opportunity the product were supplemented with Optizym enzyme to  feed the animal.The enzyme was a cellulotic one. The objective of this experiment was to study hematology (packaged cellvolume, hemoglobin, erythrocytes, leukocytes, neutrophil, lymphocyte, monocyte, eosinophil, sedimentationrate) and clinical chemistry of blood (creatinine, urea, serum glutamic piruvat transaminase, serum glutamicoxaloacetic transaminase, glucose, and cholesterol). The experiment was conducted for 2 months at Bukit-Jimbaran, Badung, Bali. Measurements were conducted  on the packaged cell volume with the method ofmicrohematocrite, hemoglobin with the method of Hematin (Hemoglobinometer or Hemometer Sahli),erythrocytes and leukocytes with the method of Hemocytometer (improved Neubaeur), differential ofleukocytes (neutrophil, lymphocyte, monocyte, eosinophil) with the method of slide; and blood sedimentationrate with the method of Westergreen, while measurements of the creatinine, urea, serum glutamic piruvattransaminase, serum glutamic oxaloacetic transaminase, glucose, and cholesterol were conducted with Reflotron Plus modified Reflovet Plus (Roch).Data were analyzed with Analysis of Variance. Results ofthe experiment showed that supplementation of the optizym for 0,25 kg and 0,50 kg in every 100 kg basaldiet of agriculture by products (unconventional) were not increased significantly on the packaged cellvolume, hemoglobin, total erythrocytes, total leucocytes, neuthrophil, lymposite, monocyte and the samething was also occurred on serum glutamic piruvat transaminase, serum glutamic oxaloacetictransaminase, glucose, and cholesterol of the animals (P > 0.05) compared to the untreated one; while therest (blood sedimentation rate, creatinine, and urea) were on the other way around (P < 0.05). However,they were not affected their performances. The animals still look healthy.  It can be concluded that thesupplementation of the optizym in this case was no effect to hematology and clinical chemistry of blood.
Co-Authors A. W. Puger Anak Agung Sagung Dewi Ayu Pratiwi, Ayu Eny Puspani I Dewa Ketut Harya Putra I G.L. Oka I Gede Suarta I Gusti Agung Arta Putra I Gusti Lanang Oka I Made Nuriyasa I N. T. ARIANA I N.S. Sutarpa, I N.S. I Nyoman Sumerta Miwada I WAYAN SUBERATA Ida Bagus Gaga Partama Ida Bagus Mantra IK. Sukada, IK. M.Pd S.T. S.Pd. I Gde Wawan Sudatha . Ni Luh Gde Sumardani Ni Luh Putu Sriyani Ni Wayan Siti Nyoman Suyasa SA. Lindawati, SA. Suranjaya I .Gd