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All Journal AGRITROP Buletin Udayana Mengabdi Majalah Ilmiah Peternakan Pastura; Journal Of Tropical Forage Science Jurnal Peternakan Tropika PTK: Jurnal Tindakan Kelas
I WAYAN SUBERATA
Fakultas Peternakan Universitas Udayana, Denpasar Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Veterinary Other

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TRANSFORMASI GEN ALBUMIN BUNGA MATAHARI (HELIANTHUS ANNUUS L.) PADA TANAMAN KEDELAI MENGGUNAKAN VEKTOR AGROBAKTERIUM TUMEFACIENS LBA4404 (PAL4404, PSW600) SECARA IN VITRO SUBERATA, I WAYAN
AGRITROP Vol. 26, No. 2 Juni 2007
Publisher : AGRITROP

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Abstract

Usages of A.tumefaciens for the purpose of including DNA sunflower albumine into soybean cell, constituted of natural ability of A. tumefaciens to trasformation in specific form of DNA plasmid (T-DNA) into crop cell later, and then integrate at mains crop cell genome. Result of gene cloning of albumine grown at bacterium of E.coli, then carried over by A. tumefaciens LBA4404, passing method of triparental mating. A. tumefaciens LBA4404 (pAL4404, pSW600) yielded later, then transformation at soybean by in vitro. Result of analysis of transformation at soybean of transgenic by in vitro, can be proved with testing its resilience to gene activity of GUS-its and canamisin.
TEKNIK DAN MANAJEMEN PRODUKSI BIBIT BABI DI BANJAR DAUH UMA, DESA PENGOTAN, KABUPATEN BANGLI NLG SUMARDANI; IGA ARTA PUTRA; IW SUBERATA; IG SURANJAYA; DK HARYA PUTRA; WS YUPARDHI
Buletin Udayana Mengabdi Vol 14 No 1 (2015): April 2015
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

The short course of management of animal husbandry and using Artificial Insemination (AI) on swine wasconducted on September 6th 2014 at Dauh Uma Pengotan Village, Regency of Bangli. There were 15 farmers whomerged into group as participants of this activity. The aimed of this activity was to increase litter size of swinewith AI program in breeding management. The used method of activity was giving counseling about breedingand health management of animal husbandry in breeding farm, AI program, and then practiced inseminate onswine. Based on the process of short course it was concluded that, the response of participants on AI was veryenthusiastic in joining the activity.Keywords : animal husbandry, breeding, artificial insemination, litter size, swine.
PENINGKATAN JUMLAH KELAHIRAN ANAK BABI MELALUI TEKNOLOGI INSEMINASI BUATAN DI DESA TAKMUNG KLUNGKUNG SUMARDANI NLG; ARTININGSIH NM; SUBERATA IW
Buletin Udayana Mengabdi Vol 9 No 1 (2010): Volume 9 No.1 – April 2010
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

ABSTRACT The short course of using Artificial Insemination (AI) on swine was conducted on October 30th 2009 at Takmung Village, Bajarangkan District, Regency of Klungkung. There were 25 farmers who merged into group of livestock ‘Semadhi Karya’ as participants of this activity. The aimed of this activity was to increase litter size of swine with AI program. The methods of activity were : giving counseling about management of animal husbandry in breeding farm, AI program, and then practising inseminate on five of swine. Based on the process of short course it can be concluded that, the response of participants on AI was very enthusiastic in joining the activity.
PENERAPAN TEKNOLOGI FERMENTASI JERAMI PADI DENGAN SUPLEMEN CAMPURAN UREA, KAPUR DAN MOLASIS UNTUK MENINGKATKAN PRODUKSI TERNAK SAPI DI DESA BEBETIN IN. S Sutama; IK Sukada; IW Suberata; IW Wijana; IN Ardika
Buletin Udayana Mengabdi Vol 15 No 2 (2016): Jurnal Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Kegiatan pengabdian kepada masyarakat yang dilakukan di Desa Bebetin, Kecamatan Sawan, KabupatenBuleleng bertujuan untuk memberikan pengetahuan dan ketrampilan kepada petani tentang fermentasi jeramipadi dengan suplemen campuran urea, kapur, dan molasis. Ketersediaan hijauan biasanya melimpah saatpanen dan musim hujan, namun saat ketersediaan pakan terbatas petani biasanya memberikan jerami padisaja sebagai pakan ternak tanpa disertai aplikasi teknologi pengolahan. Jerami padi memiliki kandunganlignin dan silika yang tinggi sehingga sulit dicerna. Teknologi fermentasi dapat digunakan untukmeningkatkan kandungan nutrisi dan tingkat kecernaan dari jerami padi. Pelaksanaan kegiatan pengabdiankepada masyarakat dilakukan dengan dua cara, yakni melalui sosialisasi termasuk diskusi dan pelatihansingkat dengan demonstrasi langsung. Para petani sangat antusias dengan metode pelatihan terutama tentangpembuatan fermentasi jerami padi dengan suplemen campuran urea, kapur, dan molasis. Hasil dari kegiatanini, menunjukkan bahwa kesadaran dan pengetahuan para petani maupun peternak meningkat untukmemberikan suplemen pada pakan ternak. Hal ini juga akan meningkatkan konsumsi bahan pakan kering.Selain itu, persyaratan nutrisi yang terpenuhi pada pakan ternak akan berdampak pada pertumbuhan produksiternak.
OVARIUM ACTIVITIES OF LANDRACE AND BALI SOWS AT TRADITIONAL SLAUGHTER HOUSING SUMARDANI N. L. G.; I W. SUBERATA; N. M. ARTININGSIH; K. BUDAARSA
Majalah Ilmiah Peternakan Vol 21 No 2 (2018): Vol 21, No 2 (2018)
Publisher : Fakultas Peternakan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (774.428 KB) | DOI: 10.24843/MIP.2018.v21.i02.p08

Abstract

The reproductive status of sows can be analyzed based on its level of ovarian activities characterized by thepresence of a number of follicles and corpus luteum in the ovary. The study of level of ovarium activity of sowsobserved the level of other reproductive performance. In this study, 100 ovaries in each 50 Landrace and bali sowswere divided into three groups of sows weighs slaughtering as of 60-70 kg (A); 71-80 kg (B); and 80-90 kg (C). Thevariables observed were dimensions of ovary, the number of follicles and corpus luteum in the right and left ovaries.Quantitative data with quantitative t-test determines the comparison on right and left ovary activities. It showedthat the average weight of right and left ovaries in landrace sows were 5.70 ± 1.22 g; 6.77 ± 0.96 g, and 4.89 ± 1.47g; 6.13 ± 1.46 g in bali sows. The average number of dominant follicles on the right and left ovaries of landrace sowswere 6.54 ± 1.81 f follicles; 9.78 ± 1.58 follicles, and on bali sows is 5.82 ± 1.90 follicles; 8.91 ± 1.50 follicles. Thenumber of corpus luteum on the right and left ovaries of the Landrace were 5.49 ± 2.22 CL; 8.16 ± 1.86 CL, and onbali sows is 5.27 ± 2.23 CL; 7.69 ± 2.22 CL. The t-test results showed that average number of dominant follicles andcorpus luteum in left ovary were significantly greater (P <0.05) compared to the right ovary. The highest percentageof dominant follicles and corpus found in sows of slaughter grouping 80-90 kg (C). It can be concluded that leftovary activity was more active than right ovary, and ovarian activity positively correlated with ovarian weight whichindicating ovarian activity in egg cell production and reproductive hormone.
GENE TRANSFORMATION OF ALBUMINE SUNFLOWER (Helianthus annuuss L.) WITH Agrobacterium tumefaciens VECTOR BY IN PLANTA AT SOYBEAN PLANTS Suberata I. W.
Majalah Ilmiah Peternakan Vol 23 No 1 (2020): Vol. 23 No. 1 (2020)
Publisher : Fakultas Peternakan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (843.083 KB) | DOI: 10.24843/MIP.2020.v23.i01.p08

Abstract

Penggunaan A. tumefaciens untuk maksud mengintroduksi DNA albumin bunga matahari ke dalam sel tanaman kedelai didasari atas kemampuan alami dari A.tumefaciens untuk mentransfer suatu fragmen yang spesifik dari DNA plasmid (T-DNA ) ke dalam sel tanaman lalu berintegrasi pada genom sel tanaman inang. Hasil kloning gen albumin yang ditumbuhkan pada bakteri E.coli selanjutnya ditransfer ke A. tumefaciens LBA4404, melalui metode triparental mating. A. tumefaciens LBA4404 (pAL4404, pSW600 ) yang dihasilkan kemudian ditransformasikan pada tanaman kedelai secara in planta. Hasil analisis transformasi pada tanaman kedelai transgenic secara in planta dapat dibuktikan dengan analisis PCR.
EVALUASI BIJI TANAMAN GAMAL PROVENAN RETALHULEU PADA LAHAN KERING DI BUKIT BADUNG BALI A.W. Puger; I.W. Suberata; I.G.E. Putra; I.M. Suarn; I.M. Nitis
Pastura : Jurnal Ilmu Tumbuhan Pakan Ternak Vol 1 No 1
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.464 KB) | DOI: 10.24843/Pastura.2011.v01.i01.p04

Abstract

Gliricidia (Gliricidia sepium) provenance Retalhuleu seed garden has been established in 1992 in dry land farming at Bukit Pecatu Village, Bukit Badung Peninsula, South Bali, at 100m sea level, on red-brown mediteran calcareous-base soil, with 1552 mm rainfall, distributed during 4 months wet season and 8 months dry season. The Completely Randomized Block Design Arrangement consisted of 60 famili(F) as treatments, 6 blocks as replications and 10 plants per treatment were used in this experiment. The first harvest was carried out in November 1995 and the subsequent harvest was carried out in October-November each year. Results of 11nd harvests (16 year plant age) on seed production and quality showed that famili F12, F17 and F51 (ranking order) could be registered (priority rights) as planting.
PEROMBAKAN LIMBAH TANAMAN PANGAN OLEH BAKTERI SELULOLITIK ASAL CACING TANAH Suberata I W.; I M. Mudita; I N. S. Sutama
Jurnal Peternakan Tropika Vol 5 No 3 (2017)
Publisher : Animal Science Study Program, Faculty of Animal Husbandry, Udayana University

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Abstract

Evaluation of ability on degrading agricultural waste by eight cellulolytic bacteria isolated from earthworm (Lumbricus rubellus) had been carried out for have the best cellulolytic bacteria as weeds crops degrader. The experiment was conducted with completely randomized design (CRD) 8 treatments and 3 replications, treatments based on the eight cellulolytic bacteria isolates with the code EB1CL, EB2CL, EB3CL, EB4CL, EB5CL, EB6CL, EB7CL and EB8CL.Evaluated of degrade ability measure by diameters of clear zone on agricultural waste were rice straw, rice bran, water hyacinth and water lettuce leaves. The results showed that cellulolytic bacteria isolated from earthworm coded EB1CL has produce highest clear zone diameters (P <0.05) on rice bran and water lettuce leaves substrate of 1,351 cm (Vs 1,239 - 1,331 cm) and 1,727 cm (Vs 1,621 - 1,721 cm), whereas the bacteria isolate coded EB8CL has produce highest clear zone diameter (P <0.05) on rice straw and water hyacinth substrates that were 1.060 cm (vs. 1.010 - 1.053 cm) and 1.495 cm (vs 1.394 - 1.494 cm). The bacteria isolates coded EB2CL had the lowest degradation ability on all substrates measured. Based on the result of this research, it can be concluded that cellulolytic bacteria isolate from earthworm with code EB1CL and EB8CL have the best ability in degrading of agricultural waste. Key words: Cellulolytic Bacteria, Earthworm, Ability on degrading substrates, Agricultural Waste
PENGARUH PEMBERIAN SUPLEMEN YANG DIPRODUKSI DENGAN INOKULAN CACING TANAH DALAM RANSUM TERHADAP PENAMPILAN ITIK BALI UMUR 2-8 MINGGU Banurea M.R; I M. Mudita; I W. Suberata; I G.N. Kayana
Jurnal Peternakan Tropika Vol 4 No 1 (2016)
Publisher : Animal Science Study Program, Faculty of Animal Husbandry, Udayana University

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Abstract

Penelitian yang bertujuan untuk mengetahui pengaruh pemberian suplemen yang diproduksi melalui proses fermentasi dengan menggunakan inokulan cacing tanah dengan tingkat yang berbeda terhadap penampilan itik bali telah dilaksanakan di farm Fakultas Peternakan Universitas Udayana, Bukit Jimbaran, selama 2 bulan. Penelitian dilaksanakan menggunakan  Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 4 ulangan, dimana tiap ulangan menggunakan 4 ekor itik bali umur 2 minggu. Perlakuan yang diberikan yaitu: Pemberian ransum berbasis limbah pertanian tanpa suplemen (R0), pemberian ransum berbasis limbah pertanian disuplementasi SB0 (RBS0), pemberian ransum berbasis limbah pertanian disuplementasi SBC1 (RBS1), pemberian ransum berbasis limbah pertanian disuplementasi SBC2 (RBS2), pemberian ransum berbasis limbah pertanian disuplementasi SBC3 (RBS3), pemberian ransum berbasis limbah pertanian disuplementasi SBC4 (RBS4). Variabel yang diamati pada penelitian ini adalah pertambahan bobot badan harian, konsumsi bahan kering, konsumsi bahan organik, konsumsi serat kasar, konsumsi protein kasar dan feed conversion ratio. Hasil penelitian menunjukkan bahwa pemberian suplemen yang diproduksi dari inokulan cacing tanah berpengaruh nyata (P<0,05) terhadap pertambahan bobot badan harian, konsumsi bahan kering, konsumsibahan organik, konsumsi protein kasar dan feed conversion ratio, namun tidak berpengaruh nyata (P>0,05) terhadap konsumsi serat kasar. Hasil penelitan dapat disimpulkan bahwa pemanfaatan ransum basal dengan suplementasi suplemen yang diproduksi menggunakan inokulan cacing tanah  dengan penambahan 0,4% merupkan ransum yang terbaik.
RESPONS RUMPUT GAJAH (Pennisetum purpureum Schumach) TERHADAP APLIKASI PUPUK UREA, KOTORAN AYAM, DAN KOTORAN SAPI SEBAGAI SUMBER NITROGEN (N) A. A. P., Putra Wibawa; I G. B., Adi Parwata; I W., Wirawan; N. L. G., Sumardani; I W., Suberata
Majalah Ilmiah Peternakan Vol 17 No 2 (2014): Vol 17, No 2 (2014)
Publisher : Fakultas Peternakan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.137 KB) | DOI: 10.24843/MIP.2014.v17.i02.p01

Abstract

Penelitian ini bertujuan untuk mengetahui respons rumput gajah terhadap pupuk urea, pupuk kotoran ayam, dan pupuk kotoran sapi sebagai sumber nitrogen. Penelitian menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan yaitu P0 (tanpa pupuk), P1 (pupuk urea), P2 (pupuk kotoran ayam) dan P3 (pupuk kotoran sapi) dan setiap perlakuan diulang empat kali. Variabel yang diamati terdiri dari tinggi rumput, jumlah anakan, jumlah daun, warna daun, luas daun, berat kering (daun, batang, akar, total rumput), T/R (Top Root) ratio, LSR (Leaf Steam Ratio), LWR (Leaf Weight Ratio), LAR (Leaf Area Ratio), SLA (Spesifik Leaf Area), kadar protein kasar dan serat kasar. Hasil penelitian menunjukkan perlakuan dengan pemberian pupuk urea memberikan hasil yang tertinggi secara nyata (P<0,01) pada variabel tinggi rumput, jumlah anakan, jumlah daun, warna daun, luas daun, berat kering (daun, batang, total rumput), T/R ratio, dan kadar protein kasar, sedangkan pada variabel LSR, LWR, LAR, SLA memberikan nilai yang berbeda tidak nyata (P>0,05) di antara semua perlakuan. Disimpulkan bahwa perlakuan pupuk urea memberikan respons yang terbaik pada pertumbuhan, produksi, karakteristik produksi dan kualitas hijauan rumput gajah yang dihasilkan.
Co-Authors A. W. Puger Adi Parwata I G. B. Anak Agung Putu Putra Wibawa ARTININGSIH NM Aslamiyah, Ditya Banurea M.R DARSONO I Dewa Ketut Harya Putra I Gusti Agung Arta Putra I Gusti Ngurah Kayana I Ketut Sukada I Komang Budaarsa I M. Mudita I M. Mudita I N. S. Sutama I.G.E. Putra I.M. Nitis I.M. Suarn IK Sukada IN Ardika IN. S Sutama IW Wijana N. M. ARTININGSIH Ni Luh Gde Sumardani Ni Made Artiningsih Rasna Suranjaya I .Gd Wayan Sayang Yupardhi Wirawan I W.