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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Pengabdian Masyarakat Peternakan Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Jurnal Veteriner Nusantara
Sitompul, Yeremia Yobelanno
Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Jurnal Sain Veteriner

 1 
Swab Bukal Sebagai Bahan Sexing Piyikan Burung Kenari (Serinus canaria) dan Burung Merpati (Columba livia) Akrom, Afif Muhammad; Indarjulianto, Soedarmanto; Yanuartono, Yanuartono; Susmiati, Trini; Nururrozi, Alfarisa; Raharjo, Slamet; Permana, Rief Ghulam Satria; Sitompul, Yeremia Yobelanno
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1012.071 KB) | DOI: 10.22146/jsv.57364

Abstract

Molecular sexing for bird with polymerase chain reaction-based method have been developed, but the samples used as a sources of DNA are blood and feathers which are considerably invasive. The aim of this study was to study the efficiency of buccal swabs as a resource of DNA for sexing canary bird and pigeon. This study used 10 canaries (Serinus canaria) consisting of 6 adult canaries (3 males and 3 females) and 4 young nestling canaries (14 - to 18-day old) and 6 adults (3 males and 3 females) pigeons (Columba livia) and 7 young nestling pigeons (14- to 25-day-old). All birds were taken their buccal swab samples, then DNA were extracted, mixed with PCR-mix to be amplified for sexing genes with CHD1F/CHD1R primer pairs. The amplification results showed that all of adult male birds produced single band (± 500 bp), whereas all of adult female birds produced double bands (± 500 bp and ± 300 bp). The PCR method for nestling canaries showed 2 males and 2 females. whereas nestling pigeons 6 males and 1 female. Based on this study it can be concluded that buccal swabs are efficient as a source of DNA for birds sexing especially young nestling birds. 
Swab Bukal Sebagai Bahan Sexing Piyikan Burung Kenari (Serinus canaria) dan Burung Merpati (Columba livia) Afif Muhammad Akrom; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Trini Susmiati; Alfarisa Nururrozi; Slamet Raharjo; Rief Ghulam Satria Permana; Yeremia Yobelanno Sitompul
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.49032

Abstract

Teknik sexing pada burung secara molekuler dengan metode PCR telah banyak dikembangkan, tetapi sampel yang digunakan adalah darah dan bulu yang dianggap invasif. Penelitian ini bertujuan untuk mempelajari efisiensi sampel swab bukal sebagai sumber DNA dalam sexing dengan metode PCR. Penelitian ini menggunakan 10 ekor burung kenari (Serinus canaria) yang terdiri dari 6 ekor burung dewasa (3 jantan dan 3 betina) dan 4 ekor kenari piyikan (umur 14 – 18 hari) yang belum diketahui jenis kelaminnya serta 6 ekor merpati (Columba livia) dewasa (3 jantan dan 3 betina) dan 7 ekor merpati piyikan (umur 14 – 25 hari) yang belum diketahui jenis kelaminnya. Amplifikasi fragmen gen dilakukan menggunakan metode PCR dengan pasangan primer CHD1F/CHD1R.Hasil visualisasi produk PCR menunjukkan semua burung jantan dewasa menghasilkan satu band (± 500 bp), sedangkan burung betina dewasa menghasilkan dua band (± 500 bp dan ± 300 bp). Amplifikasi gen dari swab bukal burung kenari muda didapatkan 2 ekor jantan dan 2 ekor betina, sedangkan dari swab bukal burung merpati muda didapatkan 6 ekor jantan dan 1 ekor betina. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa sampel swab bukal terbukti efisien sebagai sumber DNA dalam sexing burung khususnya burung piyikan.
DESAIN PRIMER BERDASARKAN GEN MT-12S rRNA UNTUK MENDETEKSI CEMARAN DAGING BABI PADA PRODUK OLAHAN ASAL DAGING SAPI DENGAN METODE MULTIPLEKS-PCR Sitompul, Yeremia Yobelanno; Artanto, Sidna
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.53197

Abstract

The case of adulteration of pork in beef products, which causes fretfulness especially for muslims, is still found in Indonesia. Multiplex Polymerase Chain Reaction (Multiplex PCR) can help detecting the adulteration of pork in beef products. This method needs multiple specific primers to amplify several targeted DNA mixture in single step reaction. This study aimed to design specific primers that can be utilized to detect the adulteration of pork in beef products using Multiplex PCR method.Firstly, primers were designed in silico using bioinformatic tools which were MegAlign, Seqbuilder, and AmplifX. This design was based on mt-12s rRNA gene. The length of amplified fragment using the designed primers for cow was 91 bp, while the pig was 230 bp. Secondly, the designed primers were tested to amplify DNA of cow and pig from raw beef, pork, mixture of beef and pork, and twelve samples of their products (meatballs) to assess their ability to amplify targeted DNA spesifically.The result showed that the designed primers could amplify the targeted DNA specifically from raw beef, pork, mixture of both meat, and their products. Therefore, the designed primers evidently can be used to detect adulteration of pork in beef by Multiplex PCR method.
Co-Authors A.A. Ketut Agung Cahyawan W Afif Muhammad Akrom Akrom, Afif Muhammad Alfarisa Nururrozi Alfarisa Nururrozi, Alfarisa Almet, Julianty Amalo, Maria Dangur, Fransiska Aping Deta, Stepanus Umbu Dewi F.L Djungu Foeh, Nancy Diana Frederika Katerina Joesoef, Jayusman Arsiyanti Juni Claudia Dami Kallau, Novalino Harold Geoffrey Loe, Fhady Risckhy Malelak, Marlin Cindy Claudya Maria Aega Gelolodo Maxs Urias Ebenheizer Sanam Permana, Rief Ghulam Satria Rief Ghulam Satria Permana Sidna Artanto Sidna Artanto, Sidna Simarmata, Yohanes Timbun Raja Mangiut Ronael Slamet Raharjo Slamet Raharjo Soedarmanto Indarjulianto Tabali, Zulkifli Tarsisius Considus Tophianong Tarsisius Considus Tophianong, Tarsisius Considus Tri Utami Tri Utami Trini Susmiati Wuhan, Yustinus Oswin Primajuni Yanuartono Yanuartono Yanuartono, Yanuartono