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PENGARUH DIET PROTEIN DAN SUPLEMENTASI VITAMIN D3 PADA MASA MENYUSUI TERHADAP ERUPSI GIGI INSISIVUS SENTRALIS MANDIBULA ANAK TIKUS MALNUTRISI Riski Amalia Hidayah; Rakmawati Rakmawati; Aris Aji Kurniawan; Anindita Laksitasari; Meylida Ichsyani
Scripta Biologica Vol 10, No 1 (2023)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2023.10.1.1296

ABSTRAKMalnutrisi akibat kekurangan protein saat kehamilan berisiko melahirkan bayi berat badan lahir rendah (BBLR) dan berdampak pada pertumbuhan tulang dan gigi anak. Kekurangan protein saat kehamilan dapat menyebabkan terjadinya gangguan mineralisasi gigi, ganggguan maturasi gigi, hambatan erupsi gigi, kegoyahan gigi bahkan kehilangan gigi akibat penurunan kualitas tulang alveolar. Protein sangat dibutuhkan dalam proses metabolisme kalsium dalam tulang dan gigi. Vitamin D3 berperan dalam homeostasis kalsium dan fosfor yang dibutuhkan untuk mineralisasi jaringan keras termasuk tulang dan gigi. Tujuan dari penelitian ini adalah untuk mengetahui umur pertama erupsi dan panjang pertumbuhan gigi insisivus mandibula sentralis kanan dan kiri saat masa erupsi pada anak malnutrisi yang menyusu pada induk dengan perlakuan diet protein dan suplementasi vitamin D3. Penelitian ini termasuk jenis penelitian eksperimental laboratoris dengan rancangan post test only control group design. Sampel penelitian menggunakan 30 ekor tikus Rattus novergicus galur wistar yang dibagi menjadi 5 kelompok yaitu 2 kelompok kontrol meliputi kontrol positif (K+) berupa anak tikus dengan berat badan (BB) normal yang menyusu pada induk yang diberikan perlakuan diet protein standar dan suplementasi vitamin D dosis 0,036 IU/ hari peroral serta kontrol negatif (K-) berupa anak tikus malnutrisi yang menyusu pada induk yang diberikan perlakuan diet protein rendah selama masa menyusui. Kelompok perlakuan meliputi 3 kelompok anak tikus malnutrisi yang menyusu pada induk dengan diet protein standar dengan suplementasi vitamin D dosis 0,036 IU/ hari peroral (P1), diet protein standar dengan suplementasi vitamin D dosis 0,036 IU/ hari peroral (P2) serta diet protein standar saja selama masa menyusui. Hari pertama erupsi diukur secara visual dan panjang pertumbuhan gigi diukur pada umur 14 hari dan 22 hari dengan menggunakan sliding caliper. Hasil penelitian menunjukkan bahwa erupsi paling cepat terjadi pada kelompok K+ kemudian P1. Kelompok P2 dan P3 mengalami kecepatan erupsi yang sama yaitu pada rerata umur 10,2 hari, sedangkan kelompok K- mengalami erupsi gigi paling lambat yaitu mencapai rerata umur 11,5 hari. Pemberian diet protein standar dan vitamin D3 dosis 0,036 IU/hari peroral menunjukkan perbedaan yang bermakna terhadap panjang pertumbuhan gigi. Pemberian diet protein standar menunjukkan perbedaan bermakna terhadap panjang pertumbuhan gigi. Pemberian diet protein rendah dan vitamin D3 dosis 0,036 IU/hari peroral menunjukkan perbedaan bermakna terhadap panjang pertumbuhan gigi insisivus sentralis mandibula kiri namun tidak terdapat perbedaan yang bermakna pada regio kanan. Kombinasi diet protein standar dengan vitamin D3 dosis 0,036 IU/ hari per oral yang saat masa menyusui mempercepat waktu erupi gigi dan meningkatkan panjang pertumbuhan gigi insisivus sentralis mandibula kanan dan kiri anak tikus dari umur 14 hingga 22 hari.

Reaksi Likenoid Sebagai Akibat Penggunaan Obat Antihipertensi pada Rongga Mulut Anindita Laksitasari; Ridhofar Akbar Khusnul Abdillah; Rinawati Satrio; Maulina Triani; Aris Aji Kurniawan; Ryana Budi Purnama; Rakhmawati Rakhmawati
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 20 No 1 (2023)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v20i1.38591

A lichenoid reaction is commonly finding in the oral cavity. A lichenoid reaction may be caused by systemic drugs. Among the drugs are antihypertensive drugs. The aim of this study is to know the treatment of patient with oral lichenoid reaction. Male patient came into RSGM Unsoed complaining of white patches on the right gums near 3rd molar three months ago. The white patches were painless and approximately 0.5 in diameter. Anamnesis was presenting patients is conducted and has been shown that they take 6 years of antihypertensive drugs and are prescribed by their own physician in the city of Semarang. The patient was then instructed on how to keep the oral hygiene and be evaluated two weeks later. Patients then having a normal tension and were off drugs for the following 2 weeks and got a slightly reduced lesion when it was carried out by control. Treatment of a lichenoid reaction can be done with communication, information, and education about his condition to the patient first. Another conservative treatment of topical corticosteroids is recommended when symptomatic symptoms occur. Treatment of patients with an asymptomatic reaction can be done with communication, information, and education to the patient and drug substitution if perceived as needed. Another treatment that can be taken is by using the topical corticosteroid if the lesion is symptomatic.

Aktivitas antibiofilm ekstrak etanol kulit bawang merah (Allium cepa L.) dalam menghambat pembentukan biofilm Staphylococcus aureus ATCC 25923 Alya Ghina Rosyada; Christiana Cahyani Prihastuti; Dwi Nur Indah Sari; Setiawati Setiawati; Meylida Ichsyani; Anindita Laksitasari; Restian Febi Andini; Aris Aji Kurniawan
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 35, No 1 (2023): April 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v35i1.42451

ABSTRAKPendahuluan: Permasalahan dental maupun medis banyak disebabkan oleh biofilm bakteri di dalam rongga mulut. Salah satu bakteri penyusun biofilm yaitu Staphylococcus aureus. Bakteri ini merupakan jenis bakteri Gram positif yang bersifat patogen oportunistik dalam rongga mulut serta memiliki mekanisme pembentukan biofilm yang kompleks. Kulit bawang merah mengandung senyawa fitokimia seperti flavonoid, saponin, fenol, tanin, alkaloid, dan steroid yang dilaporkan bersifat antibiofilm sehingga berpotensi sebagai alternatif obat kumur. Penelitian ini bertujuan untuk menganalisis aktivitas antibiofilm ekstrak etanol kulit bawang merah (EKBM) konsentrasi 6,25%; 12,5%; 25%; 50%; dan 100% dalam menghambat pembentukan biofilm S. aureus. Metode: Penelitian ini meruoakan penelitian eksperimental laboratoris in vitro dengan rancangan penelitian posttest-only control group design.Ekstrak dibuat menggunakan metode maserasi etanol 96% dengan perendaman selama 15 hari. Kontrol positif penelitian ini adalah CHG 0,2% dan kontrol negatif berupa DMSO 1%. Penghambatan pembentukan biofilm diukur menggunakan Microtiter Plate Assay (MPA) dengan panjang gelombang 595 nm. Data hasil pembacaan MPA dianalisis secara statistik dengan uji Kruskal-Wallis dan uji post hoc Mann-Whitney. Hasil: Persentase penghambatan biofilm tertinggi ada pada EKBM 25% (92,86+1,45%) namun hasil ini masih lebih rendah daripada kelompok kontrol positif (94,74+0,56%) secara bermakna (p<0,05). Tidak terdapat perbedaan pengaruh yang bermakna (p<0,05) antara persentase penghambatan pembentukan biofilm oleh EKBM 12,5% (92,74+0,98%) dengan EKBM 25% secara statistik. Simpulan: Penghambatan pembentukan biofilm oleh EKBM terhadap biofilm S. aureus yang paling efektif terdapat pada konsentrasi 12,5%.Kata kunci: Allium cepa L, biofilm, kulit bawang merah, Staphylococcus aureus.ABSTRACTIntroduction: Many dental and medical problems are caused by bacterial biofilms in the oral cavity. One of the bacteria that form the biofilm is Staphylococcus aureus. This bacterium is a type of Gram-positive bacteria which is an opportunistic pathogen in the oral cavity and has a complex biofilm formation mechanism. Shallot peel contains phytochemical compounds such as flavonoids, saponins, phenols, tannins, alkaloids, and steroids which are reported as antibiofilms so that they have the potential as an alternative mouthwash. This study aims to analyzed the antibiofilm activity of ethanolic extract of shallot peel (EESP) with a concentration of 6.25%; 12.5%; 25%; 50%; and 100% in inhibiting the formation of S. aureus biofilms. Methods: This research was an experimental study. Extracts were prepared using 96% ethanol maceration method by immersion for 15 days. The positive control in this study was CHG 0.2% and the negative control was DMSO 1%. Inhibition of biofilm formation was measured using a Microtiter Plate Assay (MPA) with a wavelength of 595 nm. The data from the MPA readings were analyzed statistically by the Kruskal-Wallis test and the Mann-Whitney post hoc test. Results: The highest percentage of biofilm inhibition was in EESP 25% (92.86+1.45%) but this result was significantly lower than the positive control group (94.74+0.56%) (p<0.05). There was no significant difference (p<0.05) between the percentage of inhibition of biofilm formation of EESP 12.5% (92.74+0.98%) and EESP 25% statistically. Conclusion: The most effective inhibition of biofilm formation by EESP against S. aureus biofilms was found at a concentration of 12.5%.Keywords: Allium cepa L, biofilm, shallot peel, Staphylococcus aureus

Pengaruh Pemberian Penyuluhan dengan Kombinasi Metode Ceramah dan Media Video Edukasi terhadap Pengetahuan Kesehatan Gigi dan Mulut Balita pada Ibu di Wilayah Puskesmas Purwokerto Utara I Aditya Priagung Prakosa; Aris Aji Kurniawan; Anindita Laksitasari; Maulina Triani; Fadli Ashar
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Kesehatan gigi dan mulut anak di Indonesia masih didominasi oleh penyakit karies gigi. Prevalensi perawatan gigi merupakan hal yang sulit dilakukan oleh anak usia 3-5 tahun karena anak pada usia ini masih harus dibimbing untuk membedakan mana yang benar dan mana yang salah. Faktor yang sangat berpengaruh pada kesehatan gigi dan mulut anak balita yaitu dari tingkat pengetahuan ibu. Pengetahuan dapat ditingkatkan melalui penyuluhan. Penyuluhan dengan metode ceramah dapat mudah diterima dengan dibantu kombinasi tambahan media. Media Audio Visual merupakan salah satu alat peraga yang bersifat dapat didengar dan dapat dilihat yang dapat membantu dalam belajar mengajar yang berfungsi memperjelas atau mempermudah dalam memahami bahasa yang sedang dipelajari. Tujuan penelitian ini untuk mengetahui pengaruh pemberian penyuluhan dengan media video edukasi terhadap pengetahuan kesehatan gigi dan mulut balita pada ibu di wilayah kerja Puskesmas Purwokerto Utara I. Penelitian ini menggunakan jenis penelitian pre eksperimental dengan rancangan penelitian yang digunakan yaitu one group pre and posttest design tanpa dilakukan adanya kelompok pembanding. Sampel dilakukan pada ibu yang datang sambil melakukan imunisasi balitanya di Puskesmas Purwokerto Utara I. Kegiatan penyuluhan dilakukan dengan metode individual kepada ibu yang datang secara bergantian sambil melakukan imunisasi di Puskesmas Purwokerto Utara I. Jumlah ibu yang berkenan mengisi kegiatan hingga akhir dari mulai pre test, penyuluhan, dan post test yaitu sebanyak 20 orang. Berdasarkan hasil penelitian menunjukkan bahwa pemberian penyuluhan kesehatan gigi dan mulut dengan kombinasi metode cerama dan media video edukasi dapat berpengaruh terhadap pengetahuan ibu di wilayah Puskesmas Purwokerto Utara I sebesar 30,3%.

An Inhibition effect of immersion in effervescent garlic ethanol extract (Allium sativum L.) against Staphylococcus aureus growth on heat cured acrylic Ramadhanti, Dian Praba; Rachmani, Eka Prasasti Nur; Kurniawan, Aris Aji
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.77132

Denture stomatitis is an infection of the mucosa caused by bacteria such as Staphylococcus aureus (S. aureus) accumulating on the denture. Garlic (Allium sativum) contains antibacterial compounds that can be used as an alternative denture cleanser. The purpose of this study was to determine the inhibition effect of immersion in effervescent garlic ethanol extract (Allium sativum) against Staphylococcus aureus growth on heat cured acrylic dental plate. This research was a laboratory experiment in vitro using 40%, 50%, and 60% effervescent garlic ethanol extract. The samples were 12 pieces (n = 12) of heat cured acrylic plates which were divided into 3 treatment groups then incubated in S. aureus suspension for 24 hours and then immersed in an effervescent garlic ethanol extract for 6 hours. Bacterial colonies were counted using a colony counter and the data were analyzed using the One-way ANOVA and LSD Post hoc tests. The statistical analysis showed that the number of S. aureus colonies decreased along with an increased concentration of garlic ethanol extract. The results of the analysis showed that the 60% effervescent garlic ethanol extract had a significantly lower mean number of colonies compared to the 40% and 50% effervescent garlic ethanol extracts (p ≤ 0.05). This research concluded that effervescent garlic ethanol extract prevented the growth of S. aureus on the heat cured acrylic dental plate.

Effectiveness of vitamin D3 supplementation with protein realimentation in osteoblast and osteoclast of the maxilla of breastfed malnourished rat infants Hidayah, Riski Amalia; Kurniawan, Aris Aji; Widyaningsih, Pratiwi Nur; Rakhmawati, Rakhmawati
Majalah Kedokteran Gigi Indonesia Vol 9, No 2 (2023): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.78293

Child malnutrition can happen because of lack of nutrition during pregnancy. Malnutrition can cause low birth weight in babies. This can cause jaw and alveolar bone abnormalities that can lead to malocclusion, periodontitis, and others. This condition can affect bone cells, such as osteoblast and osteoclast. Vitamin D3 supplementation accompanied by protein realimentation may promote optimum bone growth. This research aims to investigate the effect of protein realimentation and vitamin D3 on osteoblast and osteoclast of malnourished rat pups’ maxilla. In this research, the rat pups were divided into 5 groups: a positive control group (K+), a negative control group (K-), and 3 treatment groups (P1, P2, and P3). The subjects of this research were 10 male and 30 female Wistar rats. A condition of malnutrition was induced in the rat pups and they were given vitamin D3 supplementation with protein realimentation. Rat pups in each group were euthanized on the 22nd day to observe the number of osteoclast and osteoblast in the maxilla. The results showed that the number of osteoblasts between normal and malnourished rats was significantly different (p value < 0.05), but the number of osteoclasts between rats in the control and treatment groups was not significantly different. Administration of vitamin D3 may affect the number of osteoblasts and may not affect the number of osteoclasts on bone. The effective dose of vitamin D3 is 0.36 IU/day.

Perbedaan Tingkat Pengetahuan Sebelum dan Sesudah Pemberian Informasi Kesehatan Gigi dan Mulut pada Ibu Hamil di Desa Suro, Kalibagor, Kabupaten Banyumas, Jawa Tengah Irene Putri Jayanti; Aris Aji Kurniawan; A. Haris Budi Widodo
Cakradonya Dental Journal Vol 16, No 2 (2024): Agustus 2024
Publisher : FKG Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/cdj.v16i2.33741

Pemeliharaan kesehatan gigi dan mulut yang optimal membutuhkan pengetahuan yang baik. Sebagian besar ibu hamil masih memiliki pengetahuan yang kurang terhadap kesehatan gigi dan mulut. Penelitian mengenai perbedaan tingkat pengetahuan sebelum dan setelah pemberian informasi kesehatan gigi dan mulut pada ibu hamil di Desa Suro, Kecamatan Kalibagor, Kabupaten Banyumas belum dilaksanakan sampai saat ini. Tujuan penelitian ini adalah untuk mengetahui perbedaan tingkat pengetahuan sebelum dan setelah pemberian informasi kesehatan gigi dan mulut pada ibu hamil di Desa Suro periode bulan Maret 2022. Penelitian ini adalah pre-eksperimental dengan one group pre and post-test design. Populasi penelitian ini adalah total populasi, yakni seluruh ibu hamil di Desa Suro yang berpartisipasi dalam kelas ibu hamil sebanyak 9 orang. Hasil penelitian menunjukkan bahwa rerata nilai pengetahuan kesehatan gigi dan mulut ibu hamil sebelum pemberian informasi adalah 61,1%, sedangkan setelah pemberian informasi adalah 78,9%. Hasil uji paired t-test menyatakan terdapat perbedaan bermakna antara nilai pengetahuan sebelum dan setelah pemberian informasi kesehatan gigi dan mulut pada ibu hamil di Desa Suro periode bulan Maret 2022. Kesimpulannya adalah pemberian informasi kesehatan gigi dan mulut dengan leaflet efektif dalam meningkatkan pengetahuan ibu hamil.

PREHEATING EFFECT OF GIOMER’S SURFACE HARDNESS ON DIRECT RESTORATION Dwiandhono, Irfan; Kurniawan, Aris Aji; Satrio, Rinawati; Logamarta, Setiadi Warata; Winanto, Muthiary Nitzschia Nur Iswary
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Composite resin is a material that is widely used for caries restoration, enamel abrasion, and aesthetic needs because of the good fit with teeth. Restorative materials that release fluoride ions may prolong the therapeutic and protective effects of the enamel. Nowadays, composite resins have been developed a new hybrid restorative material, named giomer. Giomer containing pre-reacted glass-ionomer (PRG) which can release and recharge fluoride ions. Composite resins have a disadvantage, a presence of shrinkage during polymerization, which can cause gaps between the tooth surface and composite resin. Preheating is carried out before irradiation with conventional oven. Preheating makes the composite resin stronger, reduces viscosity makes it easier to adapt and apply, and improves mechanical properties. This study aims to determine the effect of preheating on surface hardness.Method: The research sample used is 27 pieces cylinder-shaped with 5 mm diameter and 2 mm depth. The number of treatments was divided into 3 treatment groups. The treatment groups were divided based on the preheating treatment temperature of 37Â°C, 60Â°C, and control group. The surface hardness test was carried out using Vicker's microhardness tester.Result: The statistical One Way ANOVA test showed a significant result between the groups (p<0,05). LSD test (Least Significance Difference test) showed a significant result between the treatment temperature of 37Â°C, 60Â°C, and control group (p<0,05).Conclusion: The result of the giomer surface hardness test with preheating showed a higher result than the control group. The preheating group with a temperature of 60Â°C had the highest result.

The Effect of Addition of Nano hydroxyapatite Powder of Anadara granosa Shells on Surface Roughness of Heat-cured Acrylic Resin Aris Aji Kurniawan; Dian Noviyanti Agus Imam; Helmi Hirawan
Denta Journal Kedokteran Gigi Vol 14 No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v14i2.5

Background: Heat-cured acrylic resin is one of the most widely used denture base materials. However, the residual monomer can reduce its mechanical properties, which affect physical properties such as surface roughness, resulting in high porosity—the results in the growth of fungal colonies. One way to improve these properties is to add a reinforcement material such as nano-hydroxyapatite (HAp) from the Anadara granosa shell. Nano-hydroxyapatite has a large surface area to act as a filler because of its strong bonding capacity and a smoother surface, and a higher density to improve mechanical properties. Purpose: This study aims to determine HAp powder's effect from the Anadara granosa shells on the surface roughness of heat-cured acrylic resin. Methods: This type of research used an experimental laboratory, research design posttest-only control group design. Twenty-four samples were used and divided into three groups: the acrylic resin group with 1%, 3% blood cockles shell HAp, and acrylic resin without HAp with simple random sampling. Results: The results showed that the lowest mean value of surface roughness was in a group with the addition of 3% HAp, at 1.696 ± 0.25058 µm. One-Way ANOVA test on roughness showed a significant difference between groups with p = 0.000 (p <0.05). Conclusion: This study concludes that there is an effect of HAp on the roughness of heat-cured acrylic resin.

PREHEATING EFFECT OF GIOMER’S SURFACE HARDNESS ON DIRECT RESTORATION Dwiandhono, Irfan; Kurniawan, Aris Aji; Satrio, Rinawati; Logamarta, Setiadi Warata; Winanto, Muthiary Nitzschia Nur Iswary
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.1.95-99

Background: Composite resin is a material that is widely used for caries restoration, enamel abrasion, and aesthetic needs because of the good fit with teeth. Restorative materials that release fluoride ions may prolong the therapeutic and protective effects of the enamel. Nowadays, composite resins have been developed a new hybrid restorative material, named giomer. Giomer containing pre-reacted glass-ionomer (PRG) which can release and recharge fluoride ions. Composite resins have a disadvantage, a presence of shrinkage during polymerization, which can cause gaps between the tooth surface and composite resin. Preheating is carried out before irradiation with conventional oven. Preheating makes the composite resin stronger, reduces viscosity makes it easier to adapt and apply, and improves mechanical properties. This study aims to determine the effect of preheating on surface hardness.Method: The research sample used is 27 pieces cylinder-shaped with 5 mm diameter and 2 mm depth. The number of treatments was divided into 3 treatment groups. The treatment groups were divided based on the preheating treatment temperature of 37Â°C, 60Â°C, and control group. The surface hardness test was carried out using Vicker's microhardness tester.Result: The statistical One Way ANOVA test showed a significant result between the groups (p<0,05). LSD test (Least Significance Difference test) showed a significant result between the treatment temperature of 37Â°C, 60Â°C, and control group (p<0,05).Conclusion: The result of the giomer surface hardness test with preheating showed a higher result than the control group. The preheating group with a temperature of 60Â°C had the highest result.

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