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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Industri Pertanian E-jurnal Agro-Industri Indonesia International Journal of Renewable Energy Development Agrointek Jurnal Penelitian Pascapanen Pertanian Jurnal Pangan dan Agroindustri Biota Planta Tropika EDUFORTECH Jurnal Ilmu Nutrisi dan Teknologi Pakan Jurnal Teknologi & Industri Hasil Pertanian Menara Perkebunan International Journal of Engineering, Science and Information Technology Agroradix : Jurnal Ilmu Pertanian International Journal of Renewable Energy Development
Mulyorini Rahayuningsih
Department Of Agroindustrial Technology, Faculty Of Agricultural Techonology, Bogor Agricultural University, Darmaga Campus, Bogor, 16680
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Transportation

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Bioprospecting of Pectinase-Producing Bacteria from Marine Actinomycetes Hasanah, Neneng; Nadhifah, Hana; Rahayuningsih, Mulyorini; Atikana, Akhirta; Ratnakomala, Shanti; Lisdiyanti, Puspita; Rahmani, Nanik
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 4 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.4.689

Abstract

Pectinase is used in a variety of industries, including fruit juice production, textile processing, papermaking, biodegumming, coffee and tea manufacturing, medicines, feed, and nutraceuticals. The demand for pectinase enzymes grows year after year. Industrial applications require high-activity enzymes that can increase considerable product yields while also improving based on needs and byproduct use. Indonesia's industry continues to rely on imported pectinase enzymes. Indonesia, on the other hand, possesses megabiodiversity, particularly marine microorganisms, which have the potential to provide new enzymes with high activity for industrial applications. This work aims to undertake bioprospecting of marine actinomycetes producing pectinase enzymes that have the capacity to hydrolyze pectin polymer, both from commercial and biomass sources. A total of 20 marine actinomycetes isolates from sediment, seawater, and sponges were bioprospected, and one isolate was selected with high pectinase activity (BLH 1.20), which was then used to characterize pH, temperature, hydrolysis analysis on pectin polymers, and isolate identification using 16s rRNA analysis. The selected isolate (BLH 1.20) performed best in a sodium phosphate buffer with a pH of 6.0 and a temperature of 30°C, achieving an activity of 5.4 U/mL. The 16S rRNA analysis revealed that the isolate is from the genus Streptomyces and the species Streptomyces sampsonii. Keywords: bioprospecting, marine Actinomycetes, pectinase, Streptomyces sampsonii
DESAIN TEMPAT PENGELOLAAN LIMBAH CANGKANG TERPADU (TPLCT) SEBAGAI UPAYA PENINGKATAN NILAI TAMBAH LIMBAH PADAT RAJUNGAN Anggraeni, Elisa; Rahayuningsih, Mulyorini; Nugraha, Bambang Arif; Amalia, Kiki Puspita; Fadhlurrakhman, Ariq Rizky; Alan, Jesslyn Alvina; Faidzin, Muhammad Nur
Jurnal Teknologi Industri Pertanian Vol. 33 No. 3 (2023): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24961/j.tek.ind.pert.2023.33.3.290

Abstract

Blue swimming crab is a marine product commodity that has high economic value in Indonesia with a capacity of 47,895 kg/day, so there is a potential for shell waste of 25,187 kg/day distributed along Indonesia’s coastline. This shows that there is a high economic potential related to the increase in added value of crab shell waste. Shell waste is much needed by the fertilizer, chitin/chitosan and animal feed industries, but currently the quality of shell waste is not suitable and causes environmental pollution. This project was aimed at increasing the added value of crab shell waste which currently scattered, has various qualities and capacities, and need multi-stakeholder involvement. The solution developed was a collaborative business model in the form of TPLCT that has economic value, has a quality that is suitable for the shell processing industry, has an easy and efficient process, there is information transparency, and reduces environmental pollution. The prototype validation results show that the solution design provided is accepted by partners (APRI), stakeholders, and can answer existing problems. Based on the results of the study it can also be concluded that TPLCT is feasible from a financial perspective with criteria for NPV > 0 (Rp 201,257,103), IRR > BRI Retail SBDK (8.25%), namely 14.67%, B/C Ratio > 1 (1.3), and payback period < assuming a project age of 20 years (4.93 years). The selling price of dry shells increased for the shell, swimming legs, claws and other parts respectively Rp 1456/pcs, Rp 7573/kg, Rp 3786/kg, and Rp 2446/kg. Keywords: blue swimming crab shell, collaborative business model, agroindustry added values
Direct Ethanol Production from Breadfruit Starch (Artocarpus communis Forst.) by Engineered Simultaneous Saccharification and Fermentation (ESSF) using Microbes Consortium Farida, Iftachul; Syamsu, Khaswar; Rahayuningsih, Mulyorini
International Journal of Renewable Energy Development Vol 4, No 1 (2015): February 2015
Publisher : Center of Biomass & Renewable Energy (CBIORE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.4.1.25-31

Abstract

Breadfruit (Artocarpus communis Forst.) is one of sources for ethanol production, which has high starch content (89%). Ethanol production from breadfruit starch was conducted by Simultaneous Saccharification and Fermentation (SSF) technology using microbes consortium. The aim of the research was to examine a method to produce ethanol by SSF technology using microbes consortium at high yield and efficiency. The main research consisted of two treatments, namely normal SSF and enginereed SSF. The results showed that normal SSF using aeration and agitation during cultivation could produce ethanol at 11.15 ± 0.18 g/L, with the yield of product (Yp/s) 0.34 g ethanol/g substrate; and yield of biomass (Yx/s) 0.29 g cell/g substrate, respectively. A better result was obtained using engineered SSF in which aeration was stopped after biomass condition has reached the end of the exponential phase. The ethanol produced was 12.75 ± 0.04 g/L, with the yields of product (Yp/s) 0.41 g ethanol/g substrate, and the yield of cell (Yx/s) 0.09 g cell/g substrate.
Co-Authors Abdul Aziz Darwis Alan, Jesslyn Alvina Amalia, Kiki Puspita Ati Atul Quddus Atikana, Akhirta Devi Ambarwaty Oktavia Djumali Mangunwidjaja Dwi Setyaningsih Elisa Anggraeni, Elisa Erliza Hambali Erliza Noor Fadhlurrakhman, Ariq Rizky Faidzin, Muhammad Nur Febilian Adiwinata Febilian Adiwinata Fidriyanto, Rusli Fitri, Ainissya Ika Agustin Rusdiana Ika Agustin Rusdiana Ika Amalia Kartika Jaini Fakhrudin Kartika Kartika Khaswar Syamsu Kirana Sanggrami Sasmitaloka Michael Silaen Mohamad Yani Nadhifah, Hana NANIK RAHMANI Neneng Hasanah Ninda Ningtyas NISA RACHMANIA MUBARIK Nugraha, Bambang Arif PUSPITA LISDIYANTI Rini Purnawati RONI RIDWAN Rosa, Mutya Rozalia Rozalia Rusdiana, Ika Agustin SHANTI RATNAKOMALA Singgih Wibowo Slamet Budijanto Suprihatin Suprihatin Suprihatin Suprihatin Talapessy, Cornelia Titi Candra Sunarti