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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Industri Pertanian E-jurnal Agro-Industri Indonesia International Journal of Renewable Energy Development Agrointek Jurnal Penelitian Pascapanen Pertanian Jurnal Pangan dan Agroindustri Biota Planta Tropika EDUFORTECH Jurnal Ilmu Nutrisi dan Teknologi Pakan Jurnal Teknologi & Industri Hasil Pertanian Menara Perkebunan International Journal of Engineering, Science and Information Technology Agroradix : Jurnal Ilmu Pertanian International Journal of Renewable Energy Development
Mulyorini Rahayuningsih
Department Of Agroindustrial Technology, Faculty Of Agricultural Techonology, Bogor Agricultural University, Darmaga Campus, Bogor, 16680
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Transportation

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Improvement of purification process of stevia extract by combination of microfiltration and ultrafiltration Michael Silaen; Erliza Noor; Mulyorini Rahayuningsih
Menara Perkebunan Vol. 91 No. 1 (2023): 91 (1), 2023
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v91i1.521

Abstract

Microfiltration and ultrafiltration are used for the purification process of stevia extract to retain steviosides and remove tannins. The main objective of this study was to obtain the operating conditions for the purification process of stevia extract that resulted in the lowest stevioside rejection and highest tannin rejection. The purification process of stevia extract using microfiltration membrane was carried out at transmembrane pressure (1.20, 1.40, 1.65, 1.80, and 1.90 bar), cross flow velocity (0.04, 0.06, and 0.11 m s-1), and stevioside concentration of feed (7.12, 10.25, 14.03, and 18.47 g L-1). The stevia extract purification process used ultrafiltration membrane at transmembrane pressure (1.20, 1.40, 1.65, 1.80, and 1.90 bar), cross flow velocity (0.06, 0.09, and 0.12 m s-1), and stevioside concentration of feed (4.59 and 10.36 g L-1). The first step purification process was carried out using a microfiltration membrane and the resulting permeate was used as feed for the ultrafiltration process. The second step purification process was carried out using an ultrafiltration membrane. The best operating conditions of the microfiltration process were feed stevioside concentration of 14.03 g L-1 at a transmembrane pressure of 1.90 bar and a cross flow velocity of 0.11 m s-1 with a permeate flux of 82.90 L m-2 h-1. The best operating conditions of the ultrafiltration process used a feed stevioside concentration of 10.36 g L-1 with a permeate flux of 26.51 L m-2 h-1 at a transmembrane pressure of 1.90 bar and a cross flow velocity of 0.12 m s-1. The microfiltration and ultrafiltration processes resulted in total stevioside rejection of 59.52 % and total tannin rejection of 57.99 %.
Nutritional Evaluation of Quail-Based Complementary Food to Prevent Stunting in Early Childhood Rosa, Mutya; Hambali, Erliza; Rahayuningsih, Mulyorini
Jurnal Pangan dan Agroindustri Vol. 13 No. 3 (2025): Juli 2025
Publisher : Department of Food Science and Biotechnology, Faculty of Agriculture Technology, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpa.2025.013.03.2

Abstract

The quail, an endemic Indonesian poultry species, offers high nutritional value in its meat and eggs. The first 1,000 days of a child's life are crucial for optimal growth and development, with complementary foods as a key nutritional source. This study aimed to develop an instant quail-based complementary porridge enriched with various legumes. The research employed an experimental laboratory method complemented by quantitative nutritional analysis and organoleptic testing. Results indicated that the best formulation utilized soybeans, meeting the Indonesian National Standard (SNI) requirements for moisture, protein, and fat content. A 25-gram serving of the product contributes 37% of the recommended daily protein intake and provides 107 kcal of energy. The product qualifies for claims as a high-protein, high-fiber food rich in iron, vitamins, and minerals, demonstrating its potential to support infants' daily nutritional needs.
Bioprospecting of Pectinase-Producing Bacteria from Marine Actinomycetes Hasanah, Neneng; Nadhifah, Hana; Rahayuningsih, Mulyorini; Atikana, Akhirta; Ratnakomala, Shanti; Lisdiyanti, Puspita; Rahmani, Nanik
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 4 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.4.689

Abstract

Pectinase is used in a variety of industries, including fruit juice production, textile processing, papermaking, biodegumming, coffee and tea manufacturing, medicines, feed, and nutraceuticals. The demand for pectinase enzymes grows year after year. Industrial applications require high-activity enzymes that can increase considerable product yields while also improving based on needs and byproduct use. Indonesia's industry continues to rely on imported pectinase enzymes. Indonesia, on the other hand, possesses megabiodiversity, particularly marine microorganisms, which have the potential to provide new enzymes with high activity for industrial applications. This work aims to undertake bioprospecting of marine actinomycetes producing pectinase enzymes that have the capacity to hydrolyze pectin polymer, both from commercial and biomass sources. A total of 20 marine actinomycetes isolates from sediment, seawater, and sponges were bioprospected, and one isolate was selected with high pectinase activity (BLH 1.20), which was then used to characterize pH, temperature, hydrolysis analysis on pectin polymers, and isolate identification using 16s rRNA analysis. The selected isolate (BLH 1.20) performed best in a sodium phosphate buffer with a pH of 6.0 and a temperature of 30°C, achieving an activity of 5.4 U/mL. The 16S rRNA analysis revealed that the isolate is from the genus Streptomyces and the species Streptomyces sampsonii. Keywords: bioprospecting, marine Actinomycetes, pectinase, Streptomyces sampsonii
Co-Authors Abdul Aziz Darwis Ariq Rizky Fadhlurrakhman Ati Atul Quddus Atikana, Akhirta Bambang Arif Nugraha Devi Ambarwaty Oktavia Djumali Mangunwidjaja Dwi Setyaningsih Elisa Anggraeni Erliza Hambali Erliza Noor Febilian Adiwinata Febilian Adiwinata Fidriyanto, Rusli Fitri, Ainissya Iftachul Farida Ika Agustin Rusdiana Ika Agustin Rusdiana Ika Amalia Kartika Jaini Fakhrudin Jesslyn Alvina Alan Kartika Kartika Khaswar Syamsu Kiki Puspita Amalia Kirana Sanggrami Sasmitaloka Michael Silaen Mohamad Yani Muhammad Nur Faidzin Nadhifah, Hana NANIK RAHMANI Neneng Hasanah Ninda Ningtyas NISA RACHMANIA MUBARIK PUSPITA LISDIYANTI Rini Purnawati RONI RIDWAN Rosa, Mutya Rozalia Rozalia Rusdiana, Ika Agustin SHANTI RATNAKOMALA Singgih Wibowo Slamet Budijanto Suprihatin Suprihatin Suprihatin Suprihatin Talapessy, Cornelia Titi Candra Sunarti