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SEROLOGY AND MOLECULAR IDENTIFICATION OF RUBELLA VIRUS IN PEDIATRIC CATARACT AT MOEHAMMAD HOESIN PALEMBANG Rusdianto Rusdianto; Reny Violeta; Irsan Saleh
Majalah Kedokteran Sriwijaya Vol 52, No 3 (2020): Majalah Kedokteran Sriwijaya
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/mks.v52i3.12502

Congenital cataract can be found 0.6 - 6 in every 10.000 births. The most etiology is the genetic abnormality but infection is the etiology that can be controlled. Rubella infection is about 10-15 % from all etiology of congenital cataract. Rubella virus is linked with congenital rubella syndrome (CRS). Today there is no data in Indonesia about identification of rubella virus in cataract lens. The purposed of this study to identify rubella virus in children with cataract by blood serology examination and molecular (PCR from lens). The design of this study was descriptive study and this is a pilot study about rubella virus in cataract lens in Indonesia. We had 9 samples - 15 eyes with congenital cataract and juvenile cataract from July 2016 until September 2016. We did blood serological examination IgG rubella, and PCR examination from lens which was taken from aspiration intraoperative. Result: from 15 eyes; 6 eyes PCR (+) with IgG rubella (+) (40 %), 2 eyes (13.33 %) PCR (-) but IgG (+), 4 eyes (26.67 %) with PCR (+) with igG (-), and 3 eyes (20 %) d PCR (-) with IgG (-). Overall from 9 samples there are 7 samples with PCR (+) rubella and 2 samples with PCR (-). There were 7 cases with rubella positive from 9 samples in the lens from pediatric cataract patient during July 2016 until September 2016. PCR examination from cataract lens is much more sensitive than serological blood examination.

The Role of Vascular Endothelial Growth Factorin Thrombocytosis in Colorectal Cancer Patients: The Role of VEGF in Thrombocytosis Subandrate Subandrate; Dwi Indira Setyorini; Mediarty Mediarty; Irsan Saleh
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 2 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i2.45

Backgorund: Colorectal cancer was included in a group of cancer with various complications. One complication that was often a cause of morbidity and mortality was thrombocytosis. In colorectal cancer, the incidence of thrombocytosis associated with the formation of blood vessels around the tumor or angiogenesis. Factors that played an important role in angiogenesis was vascular endothelial growth factor (VEGF). Methods: This study was an observational analytic research in colorectal cancer patients to determine the correlation levels of platelets and serum VEGF levels. A total of 33 patients with colorectal cancer at the Palembang Mohammad Hoesin Hospital be research subjects to examine the levels of platelets and levels of VEGF. The level of serum VEGF was performed using ELISA technique from SIGMAÂ®. Results: The average level of the patient's platelets was281,090.9Â±105,860.8/mm3. In this study, two patients (6.06%) have thrombocytosis.The average serum levels of VEGF research subjects were 221.2 Â± 152.8 pg/mL.Correlation test of levels of serum VEGF and platelets levels showed the value of p=0.040 (p> 0.05) and r = 0468. Conclusions: Thus, it can be concluded that in this research serum VEGF levels are almost always causes an increase in platelet levels in patients with colorectal cancer.

The Anticancer Activity of Srikaya Leaves Fraction (Annona squamosa L.): An In Vitro Study Makbruri Amin; Irsan Saleh; Rachmat Hidayat
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 4 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i4.102

Abstract Introduction: Anticancer drugs are aimed primarily at inhibiting the growth and proliferation of cancer cells. Srikaya leaves (Annona squamosa L.) had been proven to possess various therapeutic effects and potential to be developed as anticancer drugs due to its cytotoxic activity. Aim of study: This study aimed to assess the anticancer activity of srikaya leaves (Annona squamosa L.) fraction. Methods: Methanol fraction of srikaya leaves were obtained at concentrations of 500; 250; 125; 62.5; 31.25 Âµg/ml. Srikaya methanol fraction and cisplatin as control were given to a plate that was sealed with T47D cells for MTT assay. Identification of compounds in the methanol fraction of srikaya leaves was performed with thin layer chromatography (TLC). Data were collected in the form of absorbance value and half-maximal inhibitory concentration (IC50) value was determined â€‹â€‹by linear regression. Data analysis was carried out with paired T test, unpaired T test, and ANOVA. Results: Average percentage of T47D cells viability increased with the decrease in the concentration of srikaya methanol fraction. Obtained IC50 value was 174.25 Âµg/ml which was quite active and potential to be developed as an anticancer drug. Methanol fraction of srikaya leaves contained secondary terpenoid metabolites, steroids, phenols, flavonoids, alkaloids and tannins. Flavonoid was the dominant metabolites in phytochemical tests and believed to play a major role in cytotoxic activity of srikaya leaves. Conclusion: Methanol fraction of srikaya leaves possessed the cytotoxic effect on T47D cancer cell line through the role of flavonoid metabolites. Keywords: srikaya, Annona squamosa, anticancer, T47D cells

The Role of Vascular Endothelial Growth Factorin Thrombocytosis in Colorectal Cancer Patients: The Role of VEGF in Thrombocytosis Subandrate Subandrate; Dwi Indira Setyorini; Mediarty Mediarty; Irsan Saleh
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 2 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i2.45

Backgorund: Colorectal cancer was included in a group of cancer with various complications. One complication that was often a cause of morbidity and mortality was thrombocytosis. In colorectal cancer, the incidence of thrombocytosis associated with the formation of blood vessels around the tumor or angiogenesis. Factors that played an important role in angiogenesis was vascular endothelial growth factor (VEGF). Methods: This study was an observational analytic research in colorectal cancer patients to determine the correlation levels of platelets and serum VEGF levels. A total of 33 patients with colorectal cancer at the Palembang Mohammad Hoesin Hospital be research subjects to examine the levels of platelets and levels of VEGF. The level of serum VEGF was performed using ELISA technique from SIGMAÂ®. Results: The average level of the patient's platelets was281,090.9Â±105,860.8/mm3. In this study, two patients (6.06%) have thrombocytosis.The average serum levels of VEGF research subjects were 221.2 Â± 152.8 pg/mL.Correlation test of levels of serum VEGF and platelets levels showed the value of p=0.040 (p> 0.05) and r = 0468. Conclusions: Thus, it can be concluded that in this research serum VEGF levels are almost always causes an increase in platelet levels in patients with colorectal cancer.

The Anticancer Activity of Srikaya Leaves Fraction (Annona squamosa L.): An In Vitro Study Makbruri Amin; Irsan Saleh; Rachmat Hidayat
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 4 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i4.102

Abstract Introduction: Anticancer drugs are aimed primarily at inhibiting the growth and proliferation of cancer cells. Srikaya leaves (Annona squamosa L.) had been proven to possess various therapeutic effects and potential to be developed as anticancer drugs due to its cytotoxic activity. Aim of study: This study aimed to assess the anticancer activity of srikaya leaves (Annona squamosa L.) fraction. Methods: Methanol fraction of srikaya leaves were obtained at concentrations of 500; 250; 125; 62.5; 31.25 Âµg/ml. Srikaya methanol fraction and cisplatin as control were given to a plate that was sealed with T47D cells for MTT assay. Identification of compounds in the methanol fraction of srikaya leaves was performed with thin layer chromatography (TLC). Data were collected in the form of absorbance value and half-maximal inhibitory concentration (IC50) value was determined â€‹â€‹by linear regression. Data analysis was carried out with paired T test, unpaired T test, and ANOVA. Results: Average percentage of T47D cells viability increased with the decrease in the concentration of srikaya methanol fraction. Obtained IC50 value was 174.25 Âµg/ml which was quite active and potential to be developed as an anticancer drug. Methanol fraction of srikaya leaves contained secondary terpenoid metabolites, steroids, phenols, flavonoids, alkaloids and tannins. Flavonoid was the dominant metabolites in phytochemical tests and believed to play a major role in cytotoxic activity of srikaya leaves. Conclusion: Methanol fraction of srikaya leaves possessed the cytotoxic effect on T47D cancer cell line through the role of flavonoid metabolites. Keywords: srikaya, Annona squamosa, anticancer, T47D cells

Effect of Pneumothorac Duration on Oxidative Stress Level of Wistar Ratâ€™s Lung Faisal Mutaqien; Bermansyah; Irsan Saleh
Sriwijaya Journal of Surgery Vol. 2 No. 2 (2019): Sriwijaya Journal of Surgery
Publisher : Surgery Department, Faculty of Medicine Universitas Sriwijaya

Abstract Introduction. Extensive pneumothorax with a long duration of symptoms can be dangerous. Broad pneumothorax can cause hypoxemia which can cause increased production of free radicals in the lung tissue (pulmonary oxidative stress). Oxidative stress in the lungs can indicate the degree of damage or disruption of cells in the lungs due to hypoxia. Methods. There were 4 groups each consisting of 6 mice: 24-hour pneumothoric group (A), 48-hour pneumothoric (B), 72-hour pneumothoric (C) and control (D). Pneumothorac is made by air injection into the right chest pleural cavity, then on an X-ray. All samples were examined for PaO2 to ascertain their level of hypotension. After that, the rats were examined for their lungs malondialdehyde (MDA) levels to find out their stress oxidation stress levels. Results. All groups of pneumothorac mice experienced hypoxemia. The average MDA level was higher in the pneumothorac group, but it was statistically significant only in the 72 hour (C) group with p = 0.031. MDA levels in each group were 1.601 Â± 0.739 (A), 1.585 Â± 0.714 (B), 2.256 Â± 0.513 (C), 1.243 Â± 0.162 (D). Conclusion. Pneumothorax can cause pulmonary oxidative stress if the pneumothorac is large in volume and the duration of symptoms reaches 72 hours. MDA can be a marker of cell disruption or injury in cases of pneumothorax who have experienced hypoxemia.

Immunological Implications of Gold Weight Implantation for Lagophthalmos: A Systematic Review Iskandar, Elza; Krisna Murti; Irsan Saleh; Ramzi Amin
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 12 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i12.1138

Background: Gold weight implantation is a well-established surgical intervention for lagophthalmos, a condition characterized by incomplete eyelid closure. While generally safe and effective, gold implants can elicit immune responses, potentially leading to complications. This systematic review aims to comprehensively evaluate the immunological implications of gold weight implantation in lagophthalmos treatment. Methods: A systematic search of PubMed and ScienceDirect databases was conducted, encompassing studies published from 2000 to June 2024. Keywords included "gold weight implant" and "gold implant AND eyelid." Studies reporting quantitative data on immune responses to gold implants were included. Data extracted included study design, patient demographics, implant characteristics, follow-up duration, complications, and long-term outcomes. Results: Thirteen studies met the inclusion criteria, encompassing a total of 340 patients (370 eyelids). Reported complications included hypersensitivity reactions (11.9%), lymphoma (0.6%), infection (1.2%), extrusion (8.1%), and nonspecific inflammatory reactions (4.3%). Hypersensitivity reactions were mediated by T-cell and B-cell responses, leading to chronic inflammation. Lymphoma cases highlighted the potential for chronic inflammation to trigger lymphoproliferative disorders. Infections were infrequent but could necessitate implant removal. Extrusion rates varied, influenced by implant size and placement. Nonspecific inflammatory reactions were observed, often requiring implant removal or steroid treatment. Conclusion: Gold weight implantation can trigger diverse immune responses, ranging from mild inflammation to severe hypersensitivity and lymphoma. Careful patient selection, meticulous surgical technique, and vigilant postoperative monitoring are crucial to minimize complications. Further research is warranted to elucidate the precise mechanisms underlying these immune responses and develop strategies for their prevention and management.

Beyond Cholesterol: The Independent Roles of Inflammation and Renal Dysfunction in Carotid Atherosclerosis Among Indonesian Elders Rukiah Chodilawati; Taufik Indrajaya; Ferry Usnizar; Sudarto; Irsan Saleh
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 9 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i9.1386

Background: Atherosclerosis remains a leading cause of mortality in aging populations, driven by a complex interplay of metabolic and inflammatory factors. While dyslipidemia is a cornerstone of risk, the contributions of systemic inflammation, marked by high-sensitivity C-reactive protein (hsCRP), and declining renal function are increasingly recognized. This study aimed to elucidate the independent associations of hsCRP, dyslipidemia, and renal function with the presence of carotid atherosclerosis in an understudied elderly Indonesian population. Methods: We conducted a single-center, case-control study at a tertiary hospital in Palembang, Indonesia, from January to June 2024. One hundred participants aged ≥60 years were enrolled from the geriatric outpatient clinic. Cases were defined by the presence of carotid plaque, identified via B-mode Doppler ultrasound, and defined according to international consensus criteria. Controls had no evidence of plaque. We performed multivariate logistic regression to identify independent predictors of atherosclerosis, including hsCRP, lipid parameters, and estimated glomerular filtration rate (eGFR). Results: After multivariable adjustment, three factors emerged as significant, independent predictors of carotid atherosclerosis. High total cholesterol (≥200 mg/dL) was the most powerful predictor, associated with a more than seven-fold increased odds of plaque (Adjusted Odds Ratio [aOR]: 7.38; 95% Confidence Interval [CI]: 2.87–18.94; p<0.001). Elevated hsCRP (≥2 mg/L) (aOR: 3.38; 95% CI: 1.33–8.59; p=0.005) and abnormal eGFR (≤90 mL/min/1.73m²) (aOR: 3.36; 95% CI: 1.10–10.22; p<0.001) were also robustly associated with atherosclerosis, each conferring over a three-fold increase in odds. Conclusion: In this elderly Indonesian study, dyslipidemia remains a dominant risk factor for carotid atherosclerosis. However, systemic inflammation (high hsCRP) and mild renal dysfunction (abnormal eGFR) are also powerful, independent contributors. These findings highlight the multifactorial nature of atherosclerosis and underscore the importance of a comprehensive risk assessment that extends beyond traditional lipid profiling to include markers of inflammation and renal health.

Beyond Cholesterol: The Independent Roles of Inflammation and Renal Dysfunction in Carotid Atherosclerosis Among Indonesian Elders Rukiah Chodilawati; Taufik Indrajaya; Ferry Usnizar; Sudarto; Irsan Saleh
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 9 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i9.1386

Background: Atherosclerosis remains a leading cause of mortality in aging populations, driven by a complex interplay of metabolic and inflammatory factors. While dyslipidemia is a cornerstone of risk, the contributions of systemic inflammation, marked by high-sensitivity C-reactive protein (hsCRP), and declining renal function are increasingly recognized. This study aimed to elucidate the independent associations of hsCRP, dyslipidemia, and renal function with the presence of carotid atherosclerosis in an understudied elderly Indonesian population. Methods: We conducted a single-center, case-control study at a tertiary hospital in Palembang, Indonesia, from January to June 2024. One hundred participants aged ≥60 years were enrolled from the geriatric outpatient clinic. Cases were defined by the presence of carotid plaque, identified via B-mode Doppler ultrasound, and defined according to international consensus criteria. Controls had no evidence of plaque. We performed multivariate logistic regression to identify independent predictors of atherosclerosis, including hsCRP, lipid parameters, and estimated glomerular filtration rate (eGFR). Results: After multivariable adjustment, three factors emerged as significant, independent predictors of carotid atherosclerosis. High total cholesterol (≥200 mg/dL) was the most powerful predictor, associated with a more than seven-fold increased odds of plaque (Adjusted Odds Ratio [aOR]: 7.38; 95% Confidence Interval [CI]: 2.87–18.94; p<0.001). Elevated hsCRP (≥2 mg/L) (aOR: 3.38; 95% CI: 1.33–8.59; p=0.005) and abnormal eGFR (≤90 mL/min/1.73m²) (aOR: 3.36; 95% CI: 1.10–10.22; p<0.001) were also robustly associated with atherosclerosis, each conferring over a three-fold increase in odds. Conclusion: In this elderly Indonesian study, dyslipidemia remains a dominant risk factor for carotid atherosclerosis. However, systemic inflammation (high hsCRP) and mild renal dysfunction (abnormal eGFR) are also powerful, independent contributors. These findings highlight the multifactorial nature of atherosclerosis and underscore the importance of a comprehensive risk assessment that extends beyond traditional lipid profiling to include markers of inflammation and renal health.

Effectiveness of Nigella sativa Addition against TNF-Alpha in Stage III and IV Breast Cancer Undergoing Doxorubicin and Cyclophosphamide Chemotherapy at Dr. Mohammad Hoesin General Hospital, Palembang, Indonesia Friyoga Syahril; Wirdah, Aisyah; Nur Qodir; Irfanuddin; Irsan Saleh; Yenny Dian Andayani; Mediarty; Norman Djamaluddin
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 2 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i2.924

Background: Breast cancer is a malignancy caused by the continuous uncontrolled growth of the component cells of the ducts or lobules of the breast gland. Globally, breast cancer is the second leading cause of death in women. Nigella sativa contains substances thymoquinone. Thymoquinone can inhibit the progression of cancer through anti-inflammatory, anti-oxidant mechanisms, inhibit proliferation, trigger apoptosis, and prevent angiogenesis. Chronic inflammation in breast cancer is mediated by one of them is TNF-alpha tumors. Elevated levels of TNF-α have a significant association with poor prognosis and progression of breast cancer. This study aims to determine the effectiveness of the additional Nigella sativa in declining TNF-α levels in breast cancer patients at Dr. Mohammad Hoesin General Hospital Palembang. Methods: This research used a randomized, open clinical trial design, which was carried out at the internal medicine hematology-oncology medical polyclinic and the surgical oncology polyclinic of Dr. Mohammad Hoesin General Hospital Palembang from January 2023 to October 2023. Data processing for data analysis used SPSS version 26 for Windows. Results: There were 36 research subjects followed during the study period and received Doxorubicin chemotherapy and cyclophosphamide (AC), which were divided into treatment groups of 18 people (chemotherapy with the addition of Nigella sativa @600 mg 2x2 capsule/ day and a control group of 18 people. A TNF-alpha examination was carried out before and after to assess the comparison before and after. From the results, it was found that TNF-alpha levels in the treatment group showed a decrease in TNF-alpha with a p-value <0.001. Conclusion: The addition of Nigella sativa has the effect of reducing TNF-alpha in breast cancer patients receiving AC chemotherapy at Dr. Mohammad Hoesin General Hospital, Palembang.

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