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All Journal Jurnal Veteriner AL KAUNIYAH Jurnal Kesehatan Masyarakat Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Inovasi Pendidikan IPA BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat EnviroScienteae Jurnal SOLMA Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Journal of Community Service and Empowerment Jurnal Kependidikan: Jurnal Hasil Penelitian dan Kajian Kepustakaan di Bidang Pendidikan, Pengajaran dan Pembelajaran Bioscientist : Jurnal Ilmiah Biologi Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat ENVIRONMENTAL OCCUPATIONAL HEALTH AND SAFETY JOURNAL Prisma Sains: Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Livestock and Animal Research Journal of Biotechnology and Natural Science Quagga: Jurnal Pendidikan dan Biologi
Moh. Mirza Nuryady
Universitas Muhammadiyah Malang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Veterinary Other

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Journal : Jurnal Veteriner

 1 
Desain Primer Spesifik Vektor Dengue Aedes aegypti Berdasarkan DNA Pengkode ITS-1, 5.8S Ribosomal RNA, dan ITS-2 Kurnia Ayu Miranti; Sri Wahyuni; Tutut Indria Permana; Diani Fatmawati; Moh Mirza Nuryady
Jurnal Veteriner Vol 24 No 1 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.1.76

Abstract

Identification of dengue hemorrhagic fever (DHF) vector species is important for vector control programs. Internal Transcribed Spacer (ITS) of DNA markers is one of the DNA markers that is generally used to identify a species. This study was aimed to obtain potential primers with specific results for Aedes aegypti mosquito species based on DNA sequences ITS-1, 5.8S Ribosomal RNA and ITS-2. This research was an in silico descriptive study using Primer3 and Primer-BLAST, as well as an in vitro confirmation stage was used the Polymerase Chain Reaction (PCR) method. The sequence of DNA data was obtained from NCBI with accession numbers GU980956.1 and ON652374.1. The results of in silico primer design obtained two pairs of potential primers, namely primer 1) Left 5’-CATTTGCTAGTCCCTCGGG-3’ Right 5’-CACCACACCACGTCTGAC-3’, and primer 2) Left 5’-CATTTGCTAGTCCCTCGGG-3’ Right 5’-CATCAACCGCGG TGTGTC- 3’. Visualization of PCR results was detected using a 1.5% agarose gel with a product size of aroind 800 bp. The conclusion of this study was that two pairs of ITS primers were obtained which had the potential to identify A. aegypti mosquitoes.
Co-Authors AA Sudharmawan, AA Agustin, Jihan Ully Agustina, Jihan Aurelia Ainur Rofieq Aisha, Aisha Aldina, Siti Nur Alimatul, Zada Amaliyah Dina Anggraeni Anggitania Dyan Anggraini Anka Mohammad Dinindra Arsya Lingga Datu Asmah Hidayati Aulia, Diva Ayu, Putri Dhiga Agung Sasongkojati Diani Fatmawati Diani Fatmawati Diani Fatmawati Diani Fatmawati Dinindra, Anka Muhammad Dwi Setyawan Elly Purwanti Elly Purwanti Erni Yohani Mahtuti Fatmawati, Diani Fauzi Ahmad Muda Findo Bayu Adji Fitrine Ekawasti, Fitrine Fuad Jaya Miharja Hidajah Rachmawati Husamah Husamah Ichsan Luqmana Indra Putra, Ichsan Luqmana Indra Iin Hindun Iin Hindun Ika Wahyuni Ikmal Reza Fahlevy Ilma Nurul Khoiriyah Inggita Utami Ivan Ardiansyah Jihan Ully Agustin Khoiriyah, Zakiyatul Khoiro, Aisha Azaria Nisa’ul Kiky Martha Ariesaka Kurnia Ayu Miranti Kurnia Ayu Miranti Kwon, Ohseok Lestari, Novi Puji Lise Chamisijatin Lisma Dahlia Lud Waluyo Lutfi, Muhammad Ahman Maliza, Rita Martha Ariesaka, Kiky Miftachur Rohma Misbahul Munir Muhammad Ahman Luthfi Setiawan Naadiya, Nurun Ndzani Latifatur Rofi’ah Nur Ilmi Dwi Sasmitasari Nur Islakhun Nisa’ Nurwidodo, Nurwidodo P. Patmawati Patmawati, P. Phandini, Intan Poncojari Wahyono Pratiwi, Ambar Purwanti, Elly Putri Ayu Irrodah Putri, Aura Febiola Afi Rr. Eko Susetyarini Sasmitasari, Nur Ilmi Dwi Savitri, Aulia Shifa Alkautsar Siti Mariyatul Qibtiyah Siti Nur Aldina Sri Wahyuni Sri Wahyuni Susetyarini, Rr Eko Tamala, Shofi Tutut Indria Permana Ulfa, Darlah Immaria Utami, Inggita Wahyuni, Sri Warkoyo, W. Zada Alimatul Mu’azah Zakiyatul Khoiriyah