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All Journal Jurnal Teknologi Laboratorium Meditory : The Journal of Medical Laboratory International Journal of Natural Science and Engineering Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Jurnal Pengabmas Masyarakat Sehat Jurnal Pengabmas Masyarakat Sehat
Ratih, Gusti Ayu Made
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Engineering Environmental Science Health Professions Nursing Public Health Veterinary Other

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The potential of traditional balinese spices against the growth of Salmonella sp in vitro Jirna, I Nyoman; Sudarmanto, I Gede; Kurniawan, Surya Bayu; Ratih, Gusti Ayu Made; Rasyid, Burhannuddin
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.200

Abstract

Prevention by utilizing medicinal plants can be used as an alternative treatment. This study aims to identify active antimicrobial substances and test the antimicrobial potential of traditional Balinese spices, namely Basa Selem, Basa Gede, Basa Wangen, Basa Bawang Jahe, and Basa Rajang against Salmonella sp bacteria in vitro. The True-experimental method with Posttest only-control design was used in this study, by intervening in the treatment group as well as the presence of positive and negative controls. The maceration method with 96% EtOH solvent was used to extract active substances and identify the levels of antimicrobial active substances. The TLC Spectrophotodensitometer instrument and the diffusion method (discs) were used to test antimicrobial potential. Data analysis was performed using the one-way ANOVA test. All five samples showed flavonoids, alkaloids, tannins, and phenols in qualitative tests. Based on quantitative test results of five samples, the highest compound content obtained in Basa Wangen (6.66 mg/ml of tannins), Basa Gede (3.74 mg/ml of flavonoids), Basa Bawang Jahe (2.49 mg/ml of tannins), Basa Selem (2.87 mg/ml of tannin), and Basa Rajang (6.96 mg/ml of flavonoids). There are differences in the antimicrobial potential of various types of traditional Balinese spices against the growth of Salmonella sp in vitro (sig = 0.037). The traditional Balinese spices have the antimicrobial potential of the intermediate category with a range of inhibition (16-20 mm) based on the NCCLS standard.
Determination of Total Flavonoid, Iron (Fe) and Chlorophyll Content of Brazilian Spinach Extract (Althernanthera sisso) based on Different Types of Extraction Solvents Heri Setyo Bekti; Ratih, Gusti Ayu Made
International Journal of Natural Science and Engineering Vol. 8 No. 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijnse.v8i1.84661

Abstract

Brazilian spinach (Alternanthera sissoo) is known to contain various bioactive compounds such as flavonoids, iron (Fe), and chlorophyll that have the potential to be utilized in the health and food fields. However, the levels of these compounds can vary depending on the type of solvent used in the extraction process, so research is needed to determine the most effective solvent. Brazilian spinach (Alternanthera sissoo) is a vegetable species originally from Brazil that has begun to be cultivated in Indonesia. It contains secondary metabolites such as flavonoids, alkaloids, steroids, and saponins. Additionally, this plant has relatively high levels of iron and chlorophyll compared to other vegetables, with chlorophyll playing an important role in treating anemia and assisting the body in utilizing iron. This study aims to analyze the most effective solvent for extracting total flavonoids, iron (Fe), and chlorophyll from Brazilian spinach, supporting its potential development as a food supplement ingredient. This research employed a quasi-experimental design using a completely randomized design (CRD). The samples were Brazilian spinach extracted with 70% ethanol, ethyl acetate, and methanol. The concentrations of the compounds were determined using UV-Vis spectrophotometry and Atomic Absorption Spectrophotometry (AAS). The results showed that methanol extraction produced the highest extract yield (10.87%), the highest iron content (10.205 mg/kg), chlorophyll content of 13,586.571 mg/kg, and total flavonoids of 900.128 mg/100gQE. Based on these findings, methanol was identified as the most effective solvent for extracting bioactive compounds from Brazilian spinach. These results imply that Brazilian spinach has strong potential to be developed as a natural source for food supplements.
Formulation and Analysis of Alcohol Content in Pineapple Infused Arak Bali with Gas Chromatography Ratih, Gusti Ayu Made; Habibah, Nur
International Journal of Natural Science and Engineering Vol. 6 No. 3 (2022): October
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.937 KB) | DOI: 10.23887/ijnse.v6i3.53031

Abstract

Gastronomic drinks are one of the local cultures that have an important role in the development of the tourism world, one of which is Arak Bali. Various products of Arak Bali infused began to be developed to enrich the flavor. One of the potential ingredients to increase scent and flavor is pineapple. This study was conducted to determine the most popular formulation and the alcohol content of Pineapple Infused Arak Bali as a new safe gastronomic drink in accordance with government regulations. The type of research used True experimental with Posttest Only Control Group Design. The sample used was pineapple infused Arak Bali that was formulated into 5 concentration variations. Furthermore, the alcohol content analysis was carried out using Gas Chromatography method. Sample F4 was selected based on organoleptic tests from 30 panelists as the most popular formula. Analysis of alcohol content showed that both samples were negative for methanol while the ethanol content in FD (34.22%) was classified as group C liquor and FU (18.08%) was classified as group B in accordance with government regulations.
Effectiveness of Giving Chitosan on Interleukin-6 and Mallondhyaldehide Levels in Wistar Rats with Chronic Periodontitis Dharmawati, I Gusti Agung Ayu; Mastra, Nyoman; Supariani, Ni Nyoman Dewi; Ratih, Gusti Ayu Made; Artini, Ni Putu Rahayu
International Journal of Natural Science and Engineering Vol. 7 No. 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijnse.v7i1.53897

Abstract

The 6th highest diseases that occur in Indonesian society include dental caries and periodontitis caused by dental plaque which is a breeding ground for bacteria such as Streptococcus mutants and Phorphyromonas gingivalis. The purpose of this study was to determine the effectiveness of chitosan on levels of Interleukin-6 and Malondhyaldehyde (MDA), as an anti-inflammatory marker using the Elisa test. The research method was a true experimental randomized posttest only control group design, with 3 treatment groups namely control, 2% chitosan gel, and 100mg/200g/bb of chitosan orally in 15 Wistar rats with periodontitis induced by Phorphyromonas gingivalis bacteria. The results showed that the mean levels of Interleukin-6 and Mallondhyaldehyde were significantly different (p<0.05) between the control group, chitosan gel treatment, and oral chitosan administration Interleukin-6 levels were the highest in the chitosan gel (8.3091±1.25570), the control average (5.8523±0.51512), the lowest oral average (3.2563±1.45952). Similarly, Mallondhyaldehyde levels were highest in the gel group (2.8850±0.19353), control group (2.2284±0.00914), and oral group (1.2943±0.60280).). Based on these results, oral chitosan was the most effective in the treatment of periodontitis.
The Influence of Temperature and Storage Time on Alcohol Content of Pineapple Infused Arak Bali Ratih, Gusti Ayu Made; Suryaningsih, Putu Ayu
International Journal of Natural Science and Engineering Vol. 7 No. 2 (2023): Juli
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijnse.v7i2.68076

Abstract

The development of gastronomic drinks from local cultures is an interesting potential, especially in terms of traditional drinks such as Balinese Arak. However, the sustainability of alcoholic beverage products such as Arak Bali can be affected by various factors, including temperature and storage time. This study aims to investigate the effect of temperature and storage time period on alcohol content in Balinese Arak infused with pineapple. This study used the Quasi Experimental method with a Complete Randomized Design (RAL) design of 2 factors, namely temperature factors and storage time factors. The results of the analysis showed that the Methanol content in the Bali Arak sample had negative results. In samples stored at 1ºC–8ºC for 7, 14, 21, and 28 days, the Ethanol content ranged from 16.38% to 17.27%. Meanwhile, samples stored at temperatures of 24ºC–30ºC during the same period had Ethanol content ranging from 17.27% to 20.49%. The results of statistical analysis using Two Way Anova show that there is a significant influence of temperature and storage time on alcohol content in Balinese Arak infused with pineapple. The results of this study provide further understanding of the factors that influence the stability and characteristics of traditional drinks such as Balinese Arak, and can be a guide in the development of sustainable and quality alcoholic beverage products.
The Management of Occupational Diseases among Farmer Groups in Bedugul Tourism Village, Tabanan Regency Krisna, Luh Ade Wilan; Ratih, Gusti Ayu Made; Suryaningsih, Putu Ayu; Antara, Agus
Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN Vol. 5 No. 3 (2024)
Publisher : Faculty of Mathematics and Natural Sciences - Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpkmt.v5i3.168

Abstract

Petani sebagai pekerja sektor informal di Indonesia mengalami berbagai masalah kesehatan terkait Penyakit Akibat Kerja (PAK). Kurangnya sosialisasi kesehatan tentang pentingnya penggunaan APD merupakan permasalahan yang perlu ditindaklanjuti. Pemberdayaan masyarakat khususnya kelompok tani di bidang kesehatan bertujuan agar petani mampu secara mandiri mengatasi permasalahan kesehatan pasca pandemi Covid-19. Total responden dalam kegiatan ini adalah 30 orang anggota kelompok tani Muda Mandiri. Kegiatan PKM dimulai dengan memberikan penyuluhan mengenai PAK dan pola hidup sehat berkualitas, pemeriksaan skrining kesehatan, dan memberikan bantuan berupa APD standar untuk petani. Kegiatan PKM berhasil meningkatkan pemahaman tentang penggunaan APD secara lengkap dalam upaya pencegahan keracunan pestisida pada petani. Tindak lanjut kegiatan adalah perlunya pendampingan berkelanjutan kepada khalayak sasaran untuk mencegah ataupun mengurangi risiko (PAK).
Edukasi Penggunaan Tablet Tambah Darah (TTD) untuk Pencegahan Anemia dan Pemeriksaan Hemoglobin pada Siswi SMPN 3 Abiansemal Badung Bali Ratih, Gusti Ayu Made; Wilan Krisna, Luh Ade; Suryaningsih, Putu Ayu
Jurnal Pengabmas Masyarakat Sehat Vol 6, No 4 (2024): OKTOBER
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jpms.v6i4.3782

Abstract

Anemia masih menjadi masalah kesehatan masyarakat sehubungan dengan prevalesinya yang tinggi dan dampaknya terhadap kesehatan. Remaja putri merupakan kelompok rawan menderita anemia karena dalam masa pertumbuhan dan setiap bulan mengalami menstruasi yang menyebabkan hilangnya zat besi. Menstruasi bulanan menyebabkan para remaja putri mudah mengalami Anemia Defisiensi Besi, yaitu kondisi dimana sel darah merah atau konsentrasi hemoglobin di dalamnya lebih rendah dari biasanya. Sehingga membuat tubuh lebih mudah lemas dan mudah untuk pingsan. Tidak berhenti sampai disitu, dampak anemia juga menyebabkan kondisi seperti: Penurunan imunitas; Penurunan konsentrasi belajar; Penurunan prestasi di sekolah; dan Penurunan kebugaran dan produktivitas. Tujuan kegiatan pengabdian masyarakat ini adalah meningkatkan pengetahuan sasaran mitra tentang pentingnya mengkonsumsi Tablet Tambah Darah secara rutin sebagai bentuk pencegahan penyakit anemia defisiensi besi. Metode Pengabdian yang dilakukan dalam kegiatan pengabdian pada masyarakat ini yaitu penyuluhan tentang anemia, pemeriksaan kadar Hb, pemberian informasi penggunaan TTD yang tepat dan pembagian TTD bagi siswi SMP. Kegiatan ini dilakukan pada bulan Juni di SMPN 3 Abiansemal. Total sasaran yang menjadi responden dalam kegiatan ini adalah 40 orang siswi SMP kelas 8. Karakteristik  responden berdasarkan umur yaitu sebagian besar berada pada usia yaitu usia 14 tahun sebayak 35 orang (87,5%). Kesimpulan dari kegiatan ini adalah terdapat peningkatan pemahaman tentang pentingnya mengkonsumsi Tablet Tambah Darah secara rutin sebagai bentuk pencegahan penyakit anemia defisiensi besi.
Edukasi Penggunaan Tablet Tambah Darah (TTD) untuk Pencegahan Anemia dan Pemeriksaan Hemoglobin pada Siswi SMPN 3 Abiansemal Badung Bali Ratih, Gusti Ayu Made; Wilan Krisna, Luh Ade; Suryaningsih, Putu Ayu
Jurnal Pengabmas Masyarakat Sehat Vol 6, No 4 (2024): OKTOBER
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jpms.v6i4.3782

Abstract

Anemia masih menjadi masalah kesehatan masyarakat sehubungan dengan prevalesinya yang tinggi dan dampaknya terhadap kesehatan. Remaja putri merupakan kelompok rawan menderita anemia karena dalam masa pertumbuhan dan setiap bulan mengalami menstruasi yang menyebabkan hilangnya zat besi. Menstruasi bulanan menyebabkan para remaja putri mudah mengalami Anemia Defisiensi Besi, yaitu kondisi dimana sel darah merah atau konsentrasi hemoglobin di dalamnya lebih rendah dari biasanya. Sehingga membuat tubuh lebih mudah lemas dan mudah untuk pingsan. Tidak berhenti sampai disitu, dampak anemia juga menyebabkan kondisi seperti: Penurunan imunitas; Penurunan konsentrasi belajar; Penurunan prestasi di sekolah; dan Penurunan kebugaran dan produktivitas. Tujuan kegiatan pengabdian masyarakat ini adalah meningkatkan pengetahuan sasaran mitra tentang pentingnya mengkonsumsi Tablet Tambah Darah secara rutin sebagai bentuk pencegahan penyakit anemia defisiensi besi. Metode Pengabdian yang dilakukan dalam kegiatan pengabdian pada masyarakat ini yaitu penyuluhan tentang anemia, pemeriksaan kadar Hb, pemberian informasi penggunaan TTD yang tepat dan pembagian TTD bagi siswi SMP. Kegiatan ini dilakukan pada bulan Juni di SMPN 3 Abiansemal. Total sasaran yang menjadi responden dalam kegiatan ini adalah 40 orang siswi SMP kelas 8. Karakteristik  responden berdasarkan umur yaitu sebagian besar berada pada usia yaitu usia 14 tahun sebayak 35 orang (87,5%). Kesimpulan dari kegiatan ini adalah terdapat peningkatan pemahaman tentang pentingnya mengkonsumsi Tablet Tambah Darah secara rutin sebagai bentuk pencegahan penyakit anemia defisiensi besi.
PHYTOCHEMICAL PROFILING AND ANTIOXIDANT PROPERTIES OF ETHANOL EXTRACT OF TAKOKAK EGGPLANT LEAVES (Solanum torvum) Mita Efianti, Ni Wayan; Ratih, Gusti Ayu Made; Astika Dewi, Ni Nyoman
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.3980

Abstract

Background: Takokak eggplant (Solanum torvum) has been traditionally used in medicine, and its secondary metabolites possess antioxidant potential.Objective: This study aimed to determine the phytochemical profiling and analyze the antioxidant properties of Takokak eggplant leaf extract (Solanum torvum) using ethanol solvent.Methods: An experimental research design was used. Antioxidant activity was tested using the DPPH method.Results:  Phytochemical screening test of the 70% ethanol extract revealed the presence of flavonoids, terpenoids, saponins, and tannins, while the 96% ethanol extract of Takokak eggplant leaves (Solanum torvum) contained only flavonoids, terpenoids, and tannins. Antioxidant activity tests showed that the IC50 (Inhibition Concentration) value of 70% ethanol extract was 49.9 ppm (very active), and 96% ethanol extract was 65.7 ppm (active). The AAI (Antioxidant Activity Index) value of the 70% ethanol extract was 0.80, while the 96% ethanol extract was 0.60.Conclusions: Takokak eggplant leaf extract (Solanum torvum) with 70% ethanol had higher phytochemical content and antioxidant activity than 96% ethanol.
HEMATOPOIETIC EFFECTS OF ETHANOLIC EXTRACT FROM Alternanthera sissoo ON ERYTHROCYTE, HEMOGLOBIN, AND HEMATOCRIT LEVELS IN ANEMIA-INDUCED FEMALE WISTAR RATS Ratih, Gusti Ayu Made; Aryastuti, Anak Agung Sri Agung; Suryaningsih, Putu Ayu; Dwi Agustini, Desak Gede; Habibah, Nur
Meditory : The Journal of Medical Laboratory Vol 13, No 2 (2025): Meditory, Volume 13 No. 2 Tahun 2025 (On Progress)
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i2.4714

Abstract

Background: Maternal anemia remains a primary global health concern due to its association with adverse pregnancy outcomes and the increased risk of iron deficiency anemia in infants, which may impair neurodevelopment and growth. Although iron supplementation is the standard therapy, its gastrointestinal side effects often limit treatment adherence. Therefore, Brazilian Spinach (Althernanthera sissoo), a plant rich in iron, flavonoids, and antioxidants, offers potential as a natural alternative.Objective: This study aimed to evaluate the hematopoietic effects of the ethanolic extract of Alternanthera sissoo on erythrocyte count, hemoglobin concentration, and hematocrit levels in female Wistar rats (Rattus norvegicus) induced with anemia.Methods: A quasi-experimental study using a completely randomized design (CRD) was conducted with three treatment doses (5, 7.5, and 10 mg/kg body weight). Hematological parameters (Erythrocyte count, Hemoglobin (Hb), and Hematocrit (HCT)) were analyzed using a Hematology Analyzer, followed by statistical testing with one-way ANOVA and Duncan’s Multiple Range Test (DMRT) at a 5% significance level.Results: The 10 mg/kg BW treatment produced the most significant improvement in erythrocyte count, Hb, and HCT levels compared to the other groups.Conclusions: Ethanolic extract of Althernanthera sissoo effectively enhances hematological indices in iron-deficient rats, showing strong potential as a natural nutraceutical supplement for preventing anemia during pregnancy.
Co-Authors Agus Antara, Agus Anak Agung Sri Agung Aryastuti Astika Dewi, Ni Nyoman Burhannuddin Dwi Agustini, Desak Gede Heri Setyo Bekti I Gusti Agung Ayu Dharmawati I Nyoman Jirna, I Nyoman Kurniawan, Surya Bayu Luh Ade Wilan Krisna mastra, nyoman Mita Efianti, Ni Wayan Ni Putu Rahayu Artini Nur Habibah Sudarmanto, I Gede Supariani, Ni Nyoman Dewi Suryaningsih, Putu Ayu Wilan Krisna, Luh Ade