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The Role of in Vitro Culture Producing Secondary Metabolites and Plant Conservation Techniques to Overcome the Environmental Crisis Sutini; Widiwurjani; Didik Utomo Pribadi; Nora Augustien
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3108

Abstract

This paper aims to examine the role of in vitro culture as a technique to produce secondary metabolites that are needed in various fields. In addition to producing secondary metabolites, in vitro culture can also be used as a plant conservation technique that can produce plantlet asexually in large numbers. This large number of plantlets besides being commercialized, its growth and development of plants is useful for overcoming the decline in environmental quality. An example of a plant will be useful as the lungs of an area and its roots will reduce the erosion of land areas. The conceptual framework of this study is that in addition to producing secondary metabolites, in vitro culture also has a function for plant conservation which in the future can prevent environmental damage. The problem in this study is the instability in the secondary metabolite biomass production and the need for optimization in the domain of conservation techniques. The method of this paper writing was extracting data information from journals, books and online sources which being analysed descriptively and qualitatively to verify the data objectively. The result of this study showed the role of in vitro culture that can produce secondary metabolites and can be implemented in plant conservation that overcome environmental crises.
Effects of Leaf Pruning and Nitrogen Fertilizer Application on the Growth and Yield of Sweet Corn (Zea mays saccharata Sturt) Riska Widiya Puteri; Didik Utomo Pribadi; Agus Sulistyono
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 14, No 3 (2025): June 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i3.781-788

Abstract

Sweet corn production in Indonesia is relatively low due to instability and many factors, including infestation of pests and diseases, inappropriate and inefficient cultivation techniques, and the varieties. Pruning the leaves below the cob and applying nitrogen fertilizer may improve yield. The aim of this research was to investigate the combined effect of leaf pruning and types of nitrogen fertilizer on the growth and yield of sweet corn plants. A plot experiment was performed in a Randomized Block Design with 2 factors, namely leaf pruning (no pruning, 1 leaf remaining, 2 leaves remaining, and 3 leaves remaining below the cob), and types of N fertilizer (Urea 4.5 g/plant, ZA fertilizer 9.6 g/plant, and KNO3 fertilizer 15.5 g/plant). Results showed that the best type of N source was KNO3 fertilizer with fresh weight of dehusked cobs (217.68 g), cob diameter (44.84mm), and sugar content (16.26%). Combination treatment of KNO3 fertilizer and the 3 leaves remaining provided the best results on the parameters of fresh weight of husked cobs (308.50 g), cob length (18.83 cm), weight of husked cobs (10.12 kg/plot), and yield of husked cobs (28.11 ton/ha). Keywords: Cultivation, Fertilizer, Nitrogen, Productivity, Sweet corn.
Pengaruh Kompos Limbah Baglog Jamur dan Zpt Hormon Organik Terhadap Pertumbuhan dan Hasil Tanaman Terong Ungu (Solanum melongena L.) Varietas Mustang F1 Prasetyo, Dina Aulia; Pribadi, Didik Utomo; Suhardjono, Hadi
Jurnal Agroekoteknologi Vol 17, No 1 (2025)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v17i1.31067

Abstract

Terong (Solanum melongena L.) merupakan tanaman sayuran yang cukup dikenal di negara Indonesia. Salah satu faktor yang mempengaruhi rendahnya hasil produksi terong adalah adanya faktor internal dan eksternal dalam pertumbuhan tanaman sehingga untuk mengoptimalkan pertumbuhan tanaman terong dapat dilakukan pemberian pupuk dan zat pengatur tumbuh yang sesuai dengan kebutuhan pertumbuhan tanaman, salah satunya adalah dengan limbah baglog jamur tiram dan ZPT hormon organik (hormonik). Tujuan penelitian untuk mengetahui pengaruh interaksi dan pengaruh utama kompos limbah baglog jamur dengan ZPT hormon organik terhadap pertumbuhan dan hasil tanaman terong (Solanum melongena L.). Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) secara faktorial yang terdiri dari dua faktor. Faktor pertama adalah pemberian kompos limbah baglog jamur (K) yang terdiri dari 3 taraf perlakuan yaitu K0: 275 g/tanaman, K1: 375 g/tanaman, K2: 475 g/tanaman, dan faktor kedua adalah pemberian ZPT Hormonik (H) yang terdiri dari 4 taraf yaitu H1: 3 ml/liter air, H2: 4 ml/liter air, H3: 5 ml/liter air, H4: 6 ml/liter air. Parameter yang diamati yaitu tinggi tanaman (cm), jumlah daun (helai), diameter batang (mm), umur saat muncul bunga (hst), jumlah bunga total per tanaman (bunga), jumlah buah per tanaman (buah), rata-rata panjang buah (cm), rata-rata diameter buah (mm), rata-rata berat buah per tanaman (gram), dan fruit set (%). Data hasil pengamatan dari masing-masing perlakuan dianalisis secara statistik dan dilanjutkan dengan uji lanjut Beda Nyata Terkecil (BNT) pada taraf 5%. Berdasarkan hasil penelitian yang telah dilakukan, dapat disimpulkan bahwa interaksi pemberian kompos baglog jamur dan ZPT hormon organik berpengaruh nyata terhadap umur muncul bunga, panjang buah panen ke 1, dan diameter buah panen 1. Pengaruh utama pemberian kompos baglog jamur 375 gr/tanaman merupakan dosis yang dapat memberikan hasil yang berbeda nyata terhadap parameter pertumbuhan dan hasil seperti: tinggi tanaman umur 14-35 HST (hari setelah transplanting), jumlah daun umur 14 dan 28 HST, dan berat buah panen ke 1 sedangkan pengaruh utama pemberian ZPT hormon organik 5 ml/l air dapat memberikan hasil yang berbeda nyata terhadap parameter panjang buah panen ke 1 sebesar 15,86 cm.