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The Role of in Vitro Culture Producing Secondary Metabolites and Plant Conservation Techniques to Overcome the Environmental Crisis Sutini; Widiwurjani; Didik Utomo Pribadi; Nora Augustien
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3108

This paper aims to examine the role of in vitro culture as a technique to produce secondary metabolites that are needed in various fields. In addition to producing secondary metabolites, in vitro culture can also be used as a plant conservation technique that can produce plantlet asexually in large numbers. This large number of plantlets besides being commercialized, its growth and development of plants is useful for overcoming the decline in environmental quality. An example of a plant will be useful as the lungs of an area and its roots will reduce the erosion of land areas. The conceptual framework of this study is that in addition to producing secondary metabolites, in vitro culture also has a function for plant conservation which in the future can prevent environmental damage. The problem in this study is the instability in the secondary metabolite biomass production and the need for optimization in the domain of conservation techniques. The method of this paper writing was extracting data information from journals, books and online sources which being analysed descriptively and qualitatively to verify the data objectively. The result of this study showed the role of in vitro culture that can produce secondary metabolites and can be implemented in plant conservation that overcome environmental crises.

Effects of Leaf Pruning and Nitrogen Fertilizer Application on the Growth and Yield of Sweet Corn (Zea mays saccharata Sturt) Riska Widiya Puteri; Didik Utomo Pribadi; Agus Sulistyono
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 14, No 3 (2025): June 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i3.781-788

Sweet corn production in Indonesia is relatively low due to instability and many factors, including infestation of pests and diseases, inappropriate and inefficient cultivation techniques, and the varieties. Pruning the leaves below the cob and applying nitrogen fertilizer may improve yield. The aim of this research was to investigate the combined effect of leaf pruning and types of nitrogen fertilizer on the growth and yield of sweet corn plants. A plot experiment was performed in a Randomized Block Design with 2 factors, namely leaf pruning (no pruning, 1 leaf remaining, 2 leaves remaining, and 3 leaves remaining below the cob), and types of N fertilizer (Urea 4.5 g/plant, ZA fertilizer 9.6 g/plant, and KNO3 fertilizer 15.5 g/plant). Results showed that the best type of N source was KNO3 fertilizer with fresh weight of dehusked cobs (217.68 g), cob diameter (44.84mm), and sugar content (16.26%). Combination treatment of KNO3 fertilizer and the 3 leaves remaining provided the best results on the parameters of fresh weight of husked cobs (308.50 g), cob length (18.83 cm), weight of husked cobs (10.12 kg/plot), and yield of husked cobs (28.11 ton/ha). Keywords: Cultivation, Fertilizer, Nitrogen, Productivity, Sweet corn.

Pengaruh Kompos Limbah Baglog Jamur dan Zpt Hormon Organik Terhadap Pertumbuhan dan Hasil Tanaman Terong Ungu (Solanum melongena L.) Varietas Mustang F1 Prasetyo, Dina Aulia; Pribadi, Didik Utomo; Suhardjono, Hadi
Jurnal Agroekoteknologi Vol 17, No 1 (2025)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v17i1.31067

Terong (Solanum melongena L.) merupakan tanaman sayuran yang cukup dikenal di negara Indonesia. Salah satu faktor yang mempengaruhi rendahnya hasil produksi terong adalah adanya faktor internal dan eksternal dalam pertumbuhan tanaman sehingga untuk mengoptimalkan pertumbuhan tanaman terong dapat dilakukan pemberian pupuk dan zat pengatur tumbuh yang sesuai dengan kebutuhan pertumbuhan tanaman, salah satunya adalah dengan limbah baglog jamur tiram dan ZPT hormon organik (hormonik). Tujuan penelitian untuk mengetahui pengaruh interaksi dan pengaruh utama kompos limbah baglog jamur dengan ZPT hormon organik terhadap pertumbuhan dan hasil tanaman terong (Solanum melongena L.). Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) secara faktorial yang terdiri dari dua faktor. Faktor pertama adalah pemberian kompos limbah baglog jamur (K) yang terdiri dari 3 taraf perlakuan yaitu K0: 275 g/tanaman, K1: 375 g/tanaman, K2: 475 g/tanaman, dan faktor kedua adalah pemberian ZPT Hormonik (H) yang terdiri dari 4 taraf yaitu H1: 3 ml/liter air, H2: 4 ml/liter air, H3: 5 ml/liter air, H4: 6 ml/liter air. Parameter yang diamati yaitu tinggi tanaman (cm), jumlah daun (helai), diameter batang (mm), umur saat muncul bunga (hst), jumlah bunga total per tanaman (bunga), jumlah buah per tanaman (buah), rata-rata panjang buah (cm), rata-rata diameter buah (mm), rata-rata berat buah per tanaman (gram), dan fruit set (%). Data hasil pengamatan dari masing-masing perlakuan dianalisis secara statistik dan dilanjutkan dengan uji lanjut Beda Nyata Terkecil (BNT) pada taraf 5%. Berdasarkan hasil penelitian yang telah dilakukan, dapat disimpulkan bahwa interaksi pemberian kompos baglog jamur dan ZPT hormon organik berpengaruh nyata terhadap umur muncul bunga, panjang buah panen ke 1, dan diameter buah panen 1. Pengaruh utama pemberian kompos baglog jamur 375 gr/tanaman merupakan dosis yang dapat memberikan hasil yang berbeda nyata terhadap parameter pertumbuhan dan hasil seperti: tinggi tanaman umur 14-35 HST (hari setelah transplanting), jumlah daun umur 14 dan 28 HST, dan berat buah panen ke 1 sedangkan pengaruh utama pemberian ZPT hormon organik 5 ml/l air dapat memberikan hasil yang berbeda nyata terhadap parameter panjang buah panen ke 1 sebesar 15,86 cm.

Effects of Leaf Pruning and Nitrogen Fertilizer Application on the Growth and Yield of Sweet Corn (Zea mays saccharata Sturt) Riska Widiya Puteri; Didik Utomo Pribadi; Agus Sulistyono
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 3 (2025): June 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i3.781-788

Sweet corn production in Indonesia is relatively low due to instability and many factors, including infestation of pests and diseases, inappropriate and inefficient cultivation techniques, and the varieties. Pruning the leaves below the cob and applying nitrogen fertilizer may improve yield. The aim of this research was to investigate the combined effect of leaf pruning and types of nitrogen fertilizer on the growth and yield of sweet corn plants. A plot experiment was performed in a Randomized Block Design with 2 factors, namely leaf pruning (no pruning, 1 leaf remaining, 2 leaves remaining, and 3 leaves remaining below the cob), and types of N fertilizer (Urea 4.5 g/plant, ZA fertilizer 9.6 g/plant, and KNO3 fertilizer 15.5 g/plant). Results showed that the best type of N source was KNO3 fertilizer with fresh weight of dehusked cobs (217.68 g), cob diameter (44.84mm), and sugar content (16.26%). Combination treatment of KNO3 fertilizer and the 3 leaves remaining provided the best results on the parameters of fresh weight of husked cobs (308.50 g), cob length (18.83 cm), weight of husked cobs (10.12 kg/plot), and yield of husked cobs (28.11 ton/ha). Keywords: Cultivation, Fertilizer, Nitrogen, Productivity, Sweet corn.

The Effect of Planting Media and Nutrient Flow on the Growth of Hydroponic Lettuce with the NFT System Ahyaniah Dewi Syabarina; Ramdan Hidayat; Didik Utomo Pribadi
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 15 No. 1 (2026): February 2026
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v15i1.223-232

Lettuce is a type of vegetable plant that is consumed by the wide community. Hydroponic lettuce cultivation has become an alternative as land area decreases as a result of land-use conversion. This research aims to determine lettuce growth which is influenced by the type of planting media and nutrient discharge. This study was conducted at the Greenhouse Emak Farm and Hidroponik Wadungasri, Waru District, Sidoarjo Regency, East Java, adopting a Split Plot Design with the main plot of nutrient solution discharge (L) consisting of 3 levels, namely L1 = 1.5 L/min; L2 = 2 L/min; L3 = 2.5 L/min. The subplot is the planting media (M) consisting of 4 types, namely M₁ = rockwool; M₂ = rice husk charcoal; M₃ = cocopeat; M₄ = fern. The observed variables comprised leaf number, leaf area, plant height, stem diameter, total plant biomass, stem and leaf biomass, root biomass, harvest index, and plant age. The results indicated that the combination of fern-based growing media and a nutrient solution flow rate of 1.5 L/min produced the most favorable outcomes for all measured parameters, with the exception of the harvest index. Nutrient flow rate of 1.5 L/min was recommended for hydroponic using fern growing media.

Optimizing Nasa Liquid Organic Fertilizer Concentration and NPK Fertilizer Dosage 16-16-16 on the Growth and Yield of Glutinous Corn (Zea mays ceratina L.) Firda Aisha Salsabila; Djarwatiningsih; Didik Utomo Pribadi
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 7 No. 2 (2025): Vol. 7 No. 2 Mei (2025): Jurnal Agronomi Tanaman Tropika (JUATIKA)
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v7i2.4365

Glutinous corn (Zea mays ceratina L.) is a distinctive variety known for its unique starch characteristics and high economic value. One of the challenges affecting glutinous corn plants' productivity is the soil's low nutrient content. To address this issue, improving the fertilization system is essential, which can be achieved by balancing the application of chemical fertilizers and organic fertilizers within the cultivation system. This study aims to identify the optimal doses of liquid organic fertilizer and NPK 16-16-16 fertilizer to enhance crop yields and improve fertilization efficiency. The research employed a factorial randomized block design (RAK) with two factors. The first factor is the dose of liquid organic fertilizer (C), which includes the following levels: control (without POC), 2 ml/l, 4 ml/l, and 6 ml/l. The second factor consists of the control (recommended dose of 4.5 g/plant) and doses of 2.25 g/plant, 3 g/plant, and 3.75 g/plant. Reason: Improved clarity, readability, and technical accuracy while maintaining the original meaning. The results indicated an interaction between the combination of liquid organic fertilizer and NPK 16-16-16 fertilizer treatments concerning the sugar content of glutinous corn. The treatment without applying liquid organic fertilizer (0 ml/l) yielded the highest cob weight without husks, measuring 211.22 g. Additionally, the application of NPK 16-16-16 fertilizer resulted in glutinous corn's most significant plant height at both 14 and 42 days after planting (DAP). It contributed positively to the sugar content of the corn.

Increasing the Growth and Yield of Java Sprouts Eggplant (Solanum melongena Var. Gelatik) by Administering Different Planting Media Compositions and Rabbit Urine LOF Dosages Rifdah Nur Safitri; Didik Utomo Pribadi; Pangesti Nugrahani
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 7 No. 3 (2025): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 7 No. 3 September 2025
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v7i3.4599

Gelatik eggplant (Solanum melongena var. Gelatik), commonly referred to as Lalap eggplant, is a variety that can be processed into a vegetable. However, its production has declined due to the inaccurate selection of planting media compositions and the inadequate application of fertilizers. Therefore, optimizing the planting media composition and applying an appropriate dosage of rabbit urine LOF are expected to improve the growth and yield of Gelatik eggplant. The purpose of this study was (1) to examine the interaction between planting media composition and rabbit urine LOF dosage, (2) to assess the effect of planting media composition, and (3) to evaluate the effect of rabbit urine LOF dosage. The experiment was conducted in Tuban, East Java, using a Randomized Block Design (RBD) with two factors. The first factor was planting media composition, consisting of four levels: (a) soil (control), (b) soil: rice husk charcoal: manure (2:1:1), (c) soil: rice husk charcoal: manure (1:2:1), and (d) soil: rice husk charcoal: manure (1:1:2). The second factor was rabbit urine LOF dosage, also with four levels: (a) NPK (control), (b) 300 ml/plant, (c) 400 ml/plant, and (d) 500 ml/plant. The results showed that the combination of soil, rice husk charcoal, and manure (1:1:2) and 300 ml/plant of rabbit urine LOF produced a significant interaction effect on the number of fruits per plant per harvest period. Furthermore, the composition of the planting media alone (soil: rice husk charcoal: manure, 1:1:2) significantly affected plant height, leaf number, fruit weight per fruit, fruit weight per plant, and total fruit weight per plant. Similarly, rabbit urine LOF dosage alone (300 ml/plant) significantly influenced plant height, number of leaves, fruit weight per fruit, fruit weight per plant, and total fruit weight per plant.

Optimization of Growth and Yield of Melon Plants (Cucumis melo L.) in Drip Irrigation Hydroponic System by Providing Various Planting Media and Doses of Magnesium Sulfate Fertilizer Firdi Ihza Rochman; Didik Utomo Pribadi; Felicitas Deru Dewanti
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 7 No. 3 (2025): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 7 No. 3 September 2025
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v8i1.4634

Melon is a horticultural commodity with high sales value in both local and export markets. Consumer demand for sweet, fresh, and high-quality melons continues to rise. This rising demand for melons requires higher productivity. To achieve higher melon productivity, advanced agricultural cultivation technologies are required, including hydroponic melon cultivation. This study aims to determine the effects of planting media and magnesium sulfate fertilizer doses on the growth and yield of hydroponic melons using a drip irrigation system. The study was conducted at the Puspa Lebo greenhouse, Lebo Village, Sidoarjo District, Sidoarjo Regency, East Java, from September to November 2024. The research employed a Completely Randomized Design (CRD) with two treatment factors. The first factor was the type of planting media, consisting of three treatments: rice husk charcoal, cocopeat, and a 1:1 mixture of rice husk charcoal and cocopeat. The second factor was the magnesium sulfate fertilizer dose, with four treatments: 0 g/plant, 15 g/plant, 30 g/plant, and 45 g/plant. The results indicated that the rice husk charcoal planting medium treatment affected the fruit weight parameter. Meanwhile, the Magnesium fertilizer dose treatment of 15 g/plant affected plant length and leaf number. The use of rice husk charcoal as a planting medium increased melon fruit weight, and it is recommended that further research explore its advantages for use with other commodities.

Increasing the Growth and Yield of Okra (Abelmoschus esculentus) Plants with the Use of PGPR ( Plant Growth Promoting Rhizobacteria ) and Gibberellin Krisna Bayu Prasetiyo; Didik Utomo Pribadi; Felicitas Deru Dewanti; Sri Wiyatiningsih
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 8 No. 1 (2026): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 8 No. 1 January 2026
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v8i1.4844

Okra (Abelmoschus esculentus) is a horticultural crop rich in minerals, antioxidants, polyphenols, and flavonoids. One of the limiting factors for okra production is inadequate nutrition. The use of Plant Growth-Promoting Rhizobacteria (PGPR) and gibberellins is a promising approach to enhance okra production. This study aimed to determine the optimal concentrations of PGPR and gibberellin combinations for improving the growth and yield of okra. The experiment was conducted from March to June 2025 at the Experimental Field of the Faculty of Agriculture, Veteran National Development University, East Java. A factorial experiment was designed using a Randomized Block Design (RBD) with two factors: PGPR concentration at four levels (0 ml/l, 10 ml/l, 15 ml/l, and 20 ml/l) and gibberellin concentration at four levels (0 ppm, 100 ppm, 150 ppm, and 200 ppm), resulting in 16 treatment combinations, each replicated three times. The interaction between PGPR and gibberellin treatments significantly affected plant height and the number of flowers. The 20 ml/l PGPR treatment had the most significant positive effect on the number of leaves between 28 and 49 days after planting and on fruit weight during the 1st, 3rd, and 5th harvests. The 200 ppm gibberellin treatment significantly accelerated flower emergence, with an average of 35.39 days to flower emergence. The combination of 20 ml/l PGPR and 200 ppm gibberellin was the most effective treatment for enhancing okra plant growth and yield.

Application of Planting Media Composition and Liquid Organic Mushroom Fertilizer Concentration Eternal Luck (JAKABA) on the Growth and Yield of Purple Eggplant (Solanum melongena L.) Rhendy Rifky Setiawan; Didik Utomo Pribadi; Hadi Suhardjono
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 8 No. 1 (2026): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 8 No. 1 January 2026
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v8i1.4942

The quality of the growing medium and nutrient availability influence eggplant growth and production. This studyaimed to determine the effects of different growing media combined with varying concentrations of liquid organicfertilizer on the growth and yield of eggplant (Solanum melongena L.). A completely randomized design (CRD)with two factors was used: planting media (soil; soil and rice husk charcoal 1:1; soil and goat manure 1:1; soil,rice husk charcoal, and goat manure 1:1:1) and Jakaba POC concentration (15, 30, 45, and 60 ml/L). The resultsshowed a significant interaction between the planting media and Jakaba POC on plant height, number of leaves at28–35 days after planting (DAP), and the number of fruits per plant. The best treatment was the combination ofsoil, rice husk charcoal, and goat manure (1:1:1) with 60 ml/L Jakaba POC. The planting medium significantlyaffected plant height and leaf number at 21, 42, and 49 DAP, as well as fruit weight per plant, while Jakaba POCinfluenced leaf number at 21, 42, and 49 DAP and fruit weight per plant. The combination of organic plantingmedia with Jakaba POC enhanced eggplant growth and yield, particularly plant height, leaf number, and fruitnumber. Based on these results, the combination of soil, rice husk charcoal, and goat manure with 60 ml/L JakabaPOC is recommended as an optimal treatment to improve eggplant growth and production by utilizing organicmaterials that enhance soil physical, chemical, and biological properties.

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