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Efektivitas Kitosan dan Ekstrak Daun Abelmoschus dalam Mengurangi Nilai COD, BOD, dan Logam Berat pada Limbah Laboratorium Kimia Rohana, Hana; Dzulummah, Zakiah; Asmoro, Cahyo Puji
Jurnal Pengelolaan Laboratorium Pendidikan Vol.8, No.1, Januari 2026
Publisher : UPT Laboratorium Terpadu, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jplp.8.1.23-32

Abstract

Chemical laboratory wastewater is classified as hazardous due to the presence of compounds that can pose serious risks to both human health and the surrounding environment. Parameters such as Chemical Oxygen Demand (COD), Biological Oxygen Demand (BOD), and heavy metal content serve as key indicators of water pollution, reflecting its quality and environmental impact. In this study, treatment of complex chemical laboratory wastewater was carried out through coagulation and flocculation processes using natural coagulants derived from chitosan and Abelmoschus leaf extract. The main objective of this research was to evaluate the effectiveness of these organic coagulants in reducing COD, BOD, and heavy metal concentrations. The experiment consisted of two stages: (1) optimization of treatment parameters using the Jar Test method, and (2) analysis of treated and untreated wastewater for COD, BOD, and heavy metals (Cr, Mn, Cu, Pb, Zn, Ni, and Fe) using Atomic Absorption Spectrophotometry (AAS). The results revealed that the treatment successfully reduced COD from 74,928 mg/L to 65,311 mg/L; Cr from 3.422 mg/L to 2.877 mg/L; Mn from 5.084 mg/L to 4.787 mg/L; Cu from 36.548 mg/L to 9.902 mg/L; Pb from 0.169 mg/L to 0.141 mg/L; Zn from 0.342 mg/L to 0.243 mg/L; Ni from 23.79 mg/L to 17.375 mg/L; and Fe from 13.615 mg/L to 7.697 mg/L. However, the BOD value increased from 383 mg/L to 453.5 mg/L, which is likely due to the introduction of organic matter from the natural coagulants and flocculants used. For improved treatment efficiency, further processes such as filtration or adsorption using activated carbon, zeolite, or bentonite, as well as biological treatment with activated sludge, are recommended..
Desain Kegiatan Praktik dan Pengembangan Video Tutorial Pengujian Anti Bakteri Menggunakan Prosedur “ANKER” Putra, Renardi Erwinsyah; Asmoro, Cahyo Puji; Hofifah, Siti Nur
Jurnal Pengelolaan Laboratorium Pendidikan Vol.8, No.1, Januari 2026
Publisher : UPT Laboratorium Terpadu, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jplp.8.1.33-46

Abstract

The design of practical activities for testing anti-bacterial sensitivity in microbiology practice, often only reach the verification stage using model recipe guidance. It is suspected that the recipe model laboratory activity design has not been able to construct student knowledge. The aim of this research is to develop a video tutorial for anti-bacterial sensitivity testing using VEE diagrams based on the ANKER procedure. The research method chosen was descriptive research through object scoring and even VEE diagrams, The research object was the design of anti-bacterial testing practice activities. Data sources were obtained from documentation studies, observations and interview surveys. Supervision video tutorials resulting from the design reconstruction of practical activities were tested on respondents and an assessment was carried out using a questionnaire survey to strengthen the research results. Scoring of the design of anti-bacterial sensitivity testing practice activities obtained 12 out of 18 points in the good category. Reconstruction of the practical activity design was carried out because the choice of tools and materials had not been identified. Next, video supervision of antibacterial testing was carried out as a result of the reconstruction of the design of practical activities. The results of the questionnaire showed that 72% of respondents thought that the video was suitable for use without improvement, and the remaining 28% thought that the video tutorial was suitable for use but needed improvement.