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Antimicrobial activity of plantaricin IIA-1A5 on whey growth medium againts Pseudomonas aeroginosa: a scanning electron microscopy (SEM) study Mutmainna, Andi; Arief, Irma Isnafia; Lestari, Ayu; Hidayat, Muhammad Nur
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.24 KB) | DOI: 10.33772/jitro.v9i2.22444

Abstract

The study aimed to understand antimicrobial properties of plantaricin derived from L. plantarum IIA-1A5 and its inhibitory mechanism against Pseudomonas aeruginosa ATCC 27853 under SEM (scanning electron microscopy) observation. The inhibition of microbial growth was evaluated according to diameter of inhibitory zone. Scanning electron microscopy was then applied to observe the microstructure. The diameter of antimicrobial activity against Pseudomonas aeruginosa ATCC 27853 reached 6.52 mm in whey medium and 8.45 mm in whey+ medium (added with sucrose, tryptone and yeast extract). The experimental result clearly demonstrated the inhibition Pseudomonas aeruginosa ATCC 27853. Observation by SEM suggested that the antimicrobial activity of Pseudomonas aeruginosa ATCC 27853 related destruction of cellular morphology. The cell membrane was destroyed as indicated by the release of nucleic acid and reduction of membrane potential. In short, our experiment provided meaningful evidence on the future use of plantaricin IIA-1A5 as food preservative through disruption of cellular membrane causing leading to cell dying.Keywords:  Plantaricin, Lactobacillus plantarun, Pseudomonas aeruginosa, antimicrobial activity
Pemanfaatan Buah Patikala (Etlingera Elatior) Terhadap Kualitas Mikrobiologi Daging Ayam Afkir Oktavia, Nanda Dwi; Majid, Irmawaty; Mutmainna, Andi
Anoa: Journal of Animal Husbandry Vol 3 No 1 (2024): Februari
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/anoa.v3i1.41546

Abstract

Patikala fruit is a local Indonesian plant that contains many secondary metabolite compounds wich can fungtion as anti bacterials. The aim of this research is to determine the use of patikala fruit (Etlingera elatior) on microbes in rejected chicken meat. The treatments in this study were P0 without treatment, P1 storege time 3 hours, P2 storage time 4 hours and P3 storage time 5 hours. The parameters measured in this research are the microbiological quality of rejected chicken meat which includes testing for Total Plate Count (TPC), Coliform, and E. coli. The resut of the research showed thet the the used of patikala fruit ekstrak (Etlingera elatior) on the microbial quality of rejected chicken meat did not have a significant effect on the Total Plate Count (TPC) test, Colliform test and E. coli test. However based on the data, patikala fruit ekstrak (Etlingera elatior) could inhibit the growth of mikroba samples of rejected chicken meat
Perendaman Menggunakan Ekstrak Buah Mengkudu (Morinda Citrifolia L.) dan Pengaruhnya terhadap Sifat Fisik Daging Kuda Lokal yang Telah Dibekukan Lestari, Ayu; Putra, Rezki Anugrah; Mutmainna, Andi; Irmawaty, Irmawaty; Jamili, Muhammad Arsan
Anoa: Journal of Animal Husbandry Vol 3 No 2 (2024): Agustus
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/anoa.v3i2.50578

Abstract

Meat that has been frozen will experience a decline in physical quality, so to keep its physical properties in good condition, processing technology is needed to maintain the physical properties of horse meat. The aim of the research was to determine the effect of soaking using noni fruit extract (Morinda citrifolia l.) on the physical properties of frozen local horse meat. The research method used was a completely randomized design with 4 treatments and 3 replications. Factor A concentration of noni fruit extract (Morinda citrifolia L.) was 0% (A0), 10% (A1), 15% (A2) 20% (A3) with a soaking time of 30 minutes. Data analysis used Analysis of Variance (ANOVA) and the Honest Significant Difference (BNJ) test. Based on the results of research regarding the effect of soaking local horse meat with noni fruit extract at different concentrations using meat that had been frozen, the results were obtained that soaking with noni fruit extract had no effect on the water holding capacity, breaking strength of the meat and pH, but had a very significant effect on shrinkage cook frozen local horse meat.
The Effectiveness of Kombucha as a Starter in the Production of Fermented Milk Beverages Ningtiyas, Weny Dwi; Irfan, Muhammad; Mukhlisah, Andi Nurul; Syah, Setiawan Putra; Rab, Samsu Alam; Mutmainna, Andi
Jurnal Agripet Vol 25, No 1 (2025): Volume 25, No. 1, April 2025
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v25i1.41474

Abstract

A symbiotic culture of kombucha (SCOBY) has the potential to act as a probiotic source of lactic acid bacteria, which can be combined with fermented beverage starters using fresh or reconstituted milk as a medium. This study aims to evaluate the effectiveness of kombucha in producing fermented milk products with microbiological characteristics. The experimental design used was a completely randomized factorial design with two treatment levels: skim milk concentration and kombucha concentration. The parameters observed included total lactic acid bacteria, pH, and total lactic acid. Data were analyzed by ANOVA using IBM SPSS Statistics program 29, and if significant effects were found, Duncan's post-hoc test was conducted. The results showed that using kombucha as a starter had a very significant effect (P0.01) on all three variables observed. The amount of lactic acid bacteria (LAB) in the fermented milk met the SNI standard, with a concentration of 7 log CFU/ml. It can be concluded that kombucha can be used as a starter in fermented milk production. Although the percentage of lactic acid levels and pH values are not yet listed in the Indonesian National Standard, both pH value and lactic acid levels showed a very significant effect. A higher percentage of kombucha starter led to higher lactic acid content, which is a result of bacterial metabolism. Additionally, a higher percentage of kombucha starter resulted in a lower pH value in the kombucha-fermented milk.
Studi Karakteristik Warna Tubuh dan Morfometrik Kuda di Pasar Kuda Jeneponto Lestari, Ayu; Syukri, Faris Makkawaru; Mutmainna, Andi
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 25 No 3 (2023): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.25.3.298-305.2023

Abstract

Pasar Kuda Jeneponto merupakan pasar khusus kuda tempat diperjualbelikan kuda yang berasal dari berbagai daerah yang merupakan jenis kuda lokal seperti kuda Sumbawa dan kuda Sandel/Sandelwood, kuda Timor, kuda Bima, maupun persilangan antar kuda lokal.Variasi tersebut diduga menyebabkan keragaman fenotipe warna tubuh dan morfometrik kuda. Perlu penelitian mengenai keragaman kuda di Pasar Kuda Jeneponto guna mengidentifikasi karakteristik kuda dan menjadi informasi dinamika populasi ternak di Pasar Kuda Jeneponto. Penelitian dilaksanakan pada Desember 2022 di Pasar Kuda Kabupaten Jeneponto. Sifat kualitatif diamati pada 128 ekor kuda sedangkan pengukuran morfometrik pada 32 ekor kuda secara purposive sampling. Morfometrik yang diukur adalah lingkar dada dan panjang badan. Hasil penelitian menunjukkan warna tubuh yang teridentifikasi yakni bay, roan, splash white, dun, tobiano, yellow dun, bay roans, black bay, liver chestnut, dan grey. Warna tubuh kuda yang dominan adalah bay yakni 46,875%, chestnut 15,625%, splash white 9,325%, dan red dun sebesar 6,25%. Sisanya variasi warna lain masing-masing 3,125%. Ukuran morfometrik lingkar dada dan panjang badan kuda secara berurutan adalah 132,64 cm dan 123,33 cm. Tingkat keseragaman morfometrik ukuran lingkar dada dan panjang badan kuda dinilai relatif tinggi. Keseluruhan karakteristik warna dan morfometrik kuda di Pasar Kuda Jeneponto menunjukkan kesamaan dengan cirri kuda lokal.
Implementasi Kurikulum Merdeka pada Pembelajaran Pendidikan Jasmani, Olahraga dan Kesehatan Hardi, Alif Aryadi; Mutmainna, Andi
JURNAL PENDIDIKAN OLAHRAGA Vol. 15 No. 3 (2025): JURNAL PENDIDIKAN OLAHRAGA
Publisher : STKIP Taman Siswa Bima

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37630/jpo.v15i3.2814

Abstract

Pengembangan kurikulum merupakan komponen penting dari setiap sistem pendidikan yang efektif. Untuk membuat siswa agar berpikir kritis, bertanya, dan berkarya secara mandiri, Kurikulum Merdeka menawarkan perspektif dan metode baru di kelas. Mengetahui bagaimana pembelajaran Pendidikan Jasmani, Olahraga, dan Kesehatan menggunakan Kurikulum Merdeka adalah tujuan dari penelitian ini. Penelitian ini menggunakan metode deskriptif kuantitatif. Secara keseluruhan, 267 individu (termasuk 1 guru PJOK) menjadi populasi penelitian. Dalam penelitian ini, teknik pengambilan sampel adalah total sampling. Informasi dikumpulkan melalui angket yang menggunakan skala Likert. Penelitian ini menemukan bahwa Implementasi Kurikulum Merdeka pada Pendidikan Jasmani, Olahraga dan Kesehatan di SMP Negeri 2 Tellu Siattinge, guru menyatakan baik. Di sisi lain, 76 siswa (28,47%) mengatakan berjalan dengan sangat baik, 179 siswa (67,04%) mengatakan baik, 12 siswa (4,49%) mengatakan berjalan dengan cukup, dan 0 siswa mengatakan kurang dan sangat kurang. Hasil penelitian ini menunjukkan bahwa implementasi Kurikulum Merdeka pada pembelajaran Pendidikan Jasmani, Olahraga, dan Kesehatan di SMP Negeri 2 Tellu Siattinge tergolong baik.
Pelaksanaan Proses Belajar Mengajar Pendidikan Jasmani Olahraga dan Kesehatan Pada Sekolah Dasar Hardi, Alif Aryadi; Mutmainna, Andi
Cokroaminoto Journal of Primary Education Vol. 7 No. 1 (2024): April 2024
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/cjpe.7.1.2024.3616

Abstract

Penelitian deskriptif ini dilaksanakan untuk mengidentifikasi efektivitas metode pengajaran dan kondisi sarana prasarana Pendidikan Jasmani, Olahraga dan Kesehatan (PJOK) di Sekolah Dasar Negeri 56 Lanca, Kabupaten Bone. Sebanyak 72 siswa terlibat sebagai populasi dalam penelitian ini, sementara sampel yang digunakan berjumlah 31 siswa. Hasil dari penelitian ini menunjukkan bahwa Proses Belajar Mengajar PJOK di lokasi penelitian dilaksanakan dengan beberapa indikator. Untuk metode pengajaran yang menggunakan demonstrasi, diperoleh kriteria sedang dengan tingkat respon rata-rata mencapai 78 kali (50%). Sementara itu, metode ceramah juga menunjukkan kriteria sedang dengan tingkat respon rata-rata sebanyak 83 kali (59,83%). Dapat disimpulkan bahwa pelaksanaan proses belajar mengajar Pendidikan Jasmani, Olahraga, dan Kesehatan (PJOK) di Sekolah Dasar Negeri 56 Lanca Kabupaten Bone termasuk dalam kategori sedang, dengan kriteria peralatan olahraga yang memiliki indikator sedang, berdasarkan rata-rata respons siswa sebanyak 72 kali atau 49,56%.
Literasi Fisik Anak Usia 13-15 Tahun di Sulawesi Selatan Bahar, Umrah; Mutmainna, Andi; Syahrir, Apriliansya; Arfanda, Poppy Elisano
Innovative: Journal Of Social Science Research Vol. 3 No. 5 (2023): Innovative: Journal of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pengetahuan adalah dasar dan landasan dari literasi fisik yang bertujuan untuk mengetahui apa yang harus dilakukan dan kapan waktu yang tepat untuk melakukannya. Upaya menguatkan literasi fisik pada anak diharapkan dapat memberikan motivasi dan percaya diri serta antusias untuk tetap melakukan aktivitas fisik. Dengan anak melakukan aktivitas fisik maka akan meningkatkan imunitas tubuh anak agar tetap bugar serta menjaga kesehatan. Tujuan dari penulisan karya tulis ilmiah ini yaitu anak usia 13-15 tahun dapat memahami tentang literasi fisik. Subjek penelitian adalah anak usia 13-15 tahun di daerah Sulawesi Selatan. Kesehatan seseorang bergantung pada aktivitas fisik yang dilakukan dengan didukung oleh gizi yang baik. Perkembangan seorang anak yang ideal adalah selarasnya antara perkembangan fisik, kognitif, afektif, sosial, dan emosional. Metode yang digunakan pada karya tulis ilmiah dalam studi ini adalah deskriptif kuantitatif dengan menggunakan kuesioner dengan jumlah pertanyaan 20 nomor beserta jawabannya. Berdasarkan hasil penelitian ini bahwa, berdasar pada 20 instrumen tes semua mengarah pada pengetahuan literasi fisik yang baik pada anak usia 13-15 tahun di Sulawesi Selatan. Mereka memiliki pemahaman yang tinggi terhadap literasi fisik. Hal ini berkaitan dengan perkembangan anak usia 13-15 tahun yang sifatnya holistik, yaitu jika tubuh seseorang sehat maka mereka akan tumbuh dan berkembang dengan optimal.
Pengaruh latihan skipping dan squat jump terhadap kemampuan smash permainan bulutangkis Anugrah, Reski; Santos, Maria Herlinda Dos; Bahar, Umrah; Mutmainna, Andi; Ilahi, Rahmat; Rijaluddin, Khalid
Jurnal Porkes Vol 6 No 2 (2023): PORKES
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29408/porkes.v6i2.22430

Abstract

This study aims to determine how the effect of skipping and squat jump training on smash ability in badminton games for male students of SMA Negeri 1 Kodeoha Extracurricular. Experimental research method using the static group pretest-posttest design. The population in the study amounted to 45 students with a research sample of 30 students. Data collection will be held twice the test, namely before being given treatment and after being given the final treatment. The results of data analysis and hypothesis testing were obtained using SPSS 20. The results of the analysis showed that there was a significant effect of skipping training on the ability to smash the game, indicated by the t value of tcount 5.527 > ttable 2.145 and p value (0.000) < 0.05. (2). There is a significant effect of squat jump training on badminton smash ability, indicated by the value of tcount 2.291 > ttable 2.145 and the results of p value (0.038) < 0.05. (3). Skipping training is more effective than squat jump on smash ability in badminton games indicated by the value of tcount 2.237 > ttable 2.048 and p value (0.033) > from 0.05. Based on the results of the analysis and discussion that has been described, it can be concluded that skipping and squat jump training have an effect on smash ability in badminton games for male extracurricular students of SMA Negeri 1 Kodeoha.
Peningkatan Estetika Objek Wisata Pesona Tanjung Malaha Kecamatan Samaturu Guna Menambah Daya Tarik Wisatawan Irmayanti, Irmayanti; Zizih, Andi Rezah Tenri; Aldy, Muh.; Maulana, Muh. Veri; Mutmainna, Andi; Sari, Nadia; Syaiful, Muhammad; Sapriyadi, Sapriyadi
Welfare : Jurnal Pengabdian Masyarakat Vol. 2 No. 2 (2024): Welfare : June 2024
Publisher : Fakultas Ekonomi dan Bisnis Islam, IAIN Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30762/welfare.v2i2.1326

Abstract

The aim of this service is to improve the aesthetics of the Pesona Tanjung Malaha tourist attraction in Malaha Village, Samaturu District, Kolaka Regency, in order to increase its tourist attraction. The method used is participatory and collaborative, inviting the community to play an active role. Activities start with field observations, interviews, coordination, and formulating alternative solutions. The activity stages include: (1) field observations at tourist locations; (2) interviews and coordination; (3) formulating alternative solutions; (4) painting signboards, cleaning beaches, and planting sea pine and sea pandan trees; and (5) evaluation of visitor behavior. The results of the activity show that the aesthetics of the tourist attraction have increased significantly. Painting signboards and landscaping helps create a more attractive environment for tourists. In conclusion, efforts to restore and rearrange tourist attractions, including painting signboards, are effective steps to improve the aesthetics of Tanjung Malaha Charm, which is expected to attract more tourists to the area.