Claim Missing Document
Check
Articles

Found 15 Documents
Search

Antimicrobial activity of plantaricin IIA-1A5 on whey growth medium againts Pseudomonas aeroginosa: a scanning electron microscopy (SEM) study Mutmainna, Andi; Arief, Irma Isnafia; Lestari, Ayu; Hidayat, Muhammad Nur
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.24 KB) | DOI: 10.33772/jitro.v9i2.22444

Abstract

The study aimed to understand antimicrobial properties of plantaricin derived from L. plantarum IIA-1A5 and its inhibitory mechanism against Pseudomonas aeruginosa ATCC 27853 under SEM (scanning electron microscopy) observation. The inhibition of microbial growth was evaluated according to diameter of inhibitory zone. Scanning electron microscopy was then applied to observe the microstructure. The diameter of antimicrobial activity against Pseudomonas aeruginosa ATCC 27853 reached 6.52 mm in whey medium and 8.45 mm in whey+ medium (added with sucrose, tryptone and yeast extract). The experimental result clearly demonstrated the inhibition Pseudomonas aeruginosa ATCC 27853. Observation by SEM suggested that the antimicrobial activity of Pseudomonas aeruginosa ATCC 27853 related destruction of cellular morphology. The cell membrane was destroyed as indicated by the release of nucleic acid and reduction of membrane potential. In short, our experiment provided meaningful evidence on the future use of plantaricin IIA-1A5 as food preservative through disruption of cellular membrane causing leading to cell dying.Keywords:  Plantaricin, Lactobacillus plantarun, Pseudomonas aeruginosa, antimicrobial activity
Pemanfaatan Buah Patikala (Etlingera Elatior) Terhadap Kualitas Mikrobiologi Daging Ayam Afkir Oktavia, Nanda Dwi; Majid, Irmawaty; Mutmainna, Andi
Anoa: Journal of Animal Husbandry Vol 3 No 1 (2024): Februari
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/anoa.v3i1.41546

Abstract

Patikala fruit is a local Indonesian plant that contains many secondary metabolite compounds wich can fungtion as anti bacterials. The aim of this research is to determine the use of patikala fruit (Etlingera elatior) on microbes in rejected chicken meat. The treatments in this study were P0 without treatment, P1 storege time 3 hours, P2 storage time 4 hours and P3 storage time 5 hours. The parameters measured in this research are the microbiological quality of rejected chicken meat which includes testing for Total Plate Count (TPC), Coliform, and E. coli. The resut of the research showed thet the the used of patikala fruit ekstrak (Etlingera elatior) on the microbial quality of rejected chicken meat did not have a significant effect on the Total Plate Count (TPC) test, Colliform test and E. coli test. However based on the data, patikala fruit ekstrak (Etlingera elatior) could inhibit the growth of mikroba samples of rejected chicken meat
Perendaman Menggunakan Ekstrak Buah Mengkudu (Morinda Citrifolia L.) dan Pengaruhnya terhadap Sifat Fisik Daging Kuda Lokal yang Telah Dibekukan Lestari, Ayu; Putra, Rezki Anugrah; Mutmainna, Andi; Irmawaty, Irmawaty; Jamili, Muhammad Arsan
Anoa: Journal of Animal Husbandry Vol 3 No 2 (2024): Agustus
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/anoa.v3i2.50578

Abstract

Meat that has been frozen will experience a decline in physical quality, so to keep its physical properties in good condition, processing technology is needed to maintain the physical properties of horse meat. The aim of the research was to determine the effect of soaking using noni fruit extract (Morinda citrifolia l.) on the physical properties of frozen local horse meat. The research method used was a completely randomized design with 4 treatments and 3 replications. Factor A concentration of noni fruit extract (Morinda citrifolia L.) was 0% (A0), 10% (A1), 15% (A2) 20% (A3) with a soaking time of 30 minutes. Data analysis used Analysis of Variance (ANOVA) and the Honest Significant Difference (BNJ) test. Based on the results of research regarding the effect of soaking local horse meat with noni fruit extract at different concentrations using meat that had been frozen, the results were obtained that soaking with noni fruit extract had no effect on the water holding capacity, breaking strength of the meat and pH, but had a very significant effect on shrinkage cook frozen local horse meat.
The Effectiveness of Kombucha as a Starter in the Production of Fermented Milk Beverages Ningtiyas, Weny Dwi; Irfan, Muhammad; Mukhlisah, Andi Nurul; Syah, Setiawan Putra; Rab, Samsu Alam; Mutmainna, Andi
Jurnal Agripet Vol 25, No 1 (2025): Volume 25, No. 1, April 2025
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v25i1.41474

Abstract

A symbiotic culture of kombucha (SCOBY) has the potential to act as a probiotic source of lactic acid bacteria, which can be combined with fermented beverage starters using fresh or reconstituted milk as a medium. This study aims to evaluate the effectiveness of kombucha in producing fermented milk products with microbiological characteristics. The experimental design used was a completely randomized factorial design with two treatment levels: skim milk concentration and kombucha concentration. The parameters observed included total lactic acid bacteria, pH, and total lactic acid. Data were analyzed by ANOVA using IBM SPSS Statistics program 29, and if significant effects were found, Duncan's post-hoc test was conducted. The results showed that using kombucha as a starter had a very significant effect (P0.01) on all three variables observed. The amount of lactic acid bacteria (LAB) in the fermented milk met the SNI standard, with a concentration of 7 log CFU/ml. It can be concluded that kombucha can be used as a starter in fermented milk production. Although the percentage of lactic acid levels and pH values are not yet listed in the Indonesian National Standard, both pH value and lactic acid levels showed a very significant effect. A higher percentage of kombucha starter led to higher lactic acid content, which is a result of bacterial metabolism. Additionally, a higher percentage of kombucha starter resulted in a lower pH value in the kombucha-fermented milk.
Studi Karakteristik Warna Tubuh dan Morfometrik Kuda di Pasar Kuda Jeneponto Lestari, Ayu; Syukri, Faris Makkawaru; Mutmainna, Andi
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 25 No 3 (2023): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.25.3.298-305.2023

Abstract

Pasar Kuda Jeneponto merupakan pasar khusus kuda tempat diperjualbelikan kuda yang berasal dari berbagai daerah yang merupakan jenis kuda lokal seperti kuda Sumbawa dan kuda Sandel/Sandelwood, kuda Timor, kuda Bima, maupun persilangan antar kuda lokal.Variasi tersebut diduga menyebabkan keragaman fenotipe warna tubuh dan morfometrik kuda. Perlu penelitian mengenai keragaman kuda di Pasar Kuda Jeneponto guna mengidentifikasi karakteristik kuda dan menjadi informasi dinamika populasi ternak di Pasar Kuda Jeneponto. Penelitian dilaksanakan pada Desember 2022 di Pasar Kuda Kabupaten Jeneponto. Sifat kualitatif diamati pada 128 ekor kuda sedangkan pengukuran morfometrik pada 32 ekor kuda secara purposive sampling. Morfometrik yang diukur adalah lingkar dada dan panjang badan. Hasil penelitian menunjukkan warna tubuh yang teridentifikasi yakni bay, roan, splash white, dun, tobiano, yellow dun, bay roans, black bay, liver chestnut, dan grey. Warna tubuh kuda yang dominan adalah bay yakni 46,875%, chestnut 15,625%, splash white 9,325%, dan red dun sebesar 6,25%. Sisanya variasi warna lain masing-masing 3,125%. Ukuran morfometrik lingkar dada dan panjang badan kuda secara berurutan adalah 132,64 cm dan 123,33 cm. Tingkat keseragaman morfometrik ukuran lingkar dada dan panjang badan kuda dinilai relatif tinggi. Keseluruhan karakteristik warna dan morfometrik kuda di Pasar Kuda Jeneponto menunjukkan kesamaan dengan cirri kuda lokal.