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All Journal Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Sainteks Jurnal Sumberdaya Lahan Menara Perkebunan

Iswandi Anas

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Author-ID : 1839931

Agriculture, Biological Sciences & Forestry Computer Science & IT Earth & Planetary Sciences Environmental Science Mechanical Engineering Medicine & Pharmacology

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EFFECT OF AZOTOBACTER AND AZOSPIRILLUM ON GROWTH AND YIELD OF RICE GROWN ON TIDAL SWAMP RICE FIELD IN SOUTH KALIMANTAN Fakhrur Razie; Iswandi Anas
Jurnal Ilmu Tanah dan Lingkungan Vol 10 No 2 (2008): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Tidal swamp land is a potential area for rice cultivation. However, tidal swamp is fragile ecosystems, so that when this area is used for rice cultivation, it has to be done carefully. To reduce a risk of environmental pollution in the tidal swamp area due to rice cultivation, the area should be managed properly and wisely especially when using agrochemicals such as fertilizers and pesticides. In relation to this, the use of bio-fertilizer such as Azotobacter or/and Azospirillum, an atmospheric nitrogen fixing bacterium, might be an important thing for this area. The objectives of this study were to evaluate the number of NFB (i.e. Azotobacter and Azospirillum spp.) found in tidalswamp rice fields of South Kalimantan, and their ability in fixing atmospheric nitrogen and supplying this fixed nitrogen to rice, and in increasing rice yields. There were three research stages performed in this study. The first, collecting, isolating and purifying the NFB on the selective media of nitrogen free media. The second, a hydroponic experiment in the greenhouse using Yoshida nutrition solution (Yoshida et al., 1976 in IRRI, 2003) as culture media and selected Azotobacter and Azospirillum. The third experiment was to study the effect of selected Azotobacter isolates to increase growth and yield of IR64 and Margasari rice cultivars, and two Azotobacter isolates were combined with six inoculation methods for Siam Unus rice cultivar as treatments. The result showed that the number of NFB strains found in rhizospere rice cultivars were varied widely. Then, Azotobacter and Azospirillum spp selected from rice fields in the tidal land of South Kalimantan when associated with IR64 and Siam Unus rice cultivars have ability in fixing atmospheric N2 and in supplying N on the initial rice growth. Azotobacter T.B.PDST.2b and T.HM.BPMT.2b were significantly supplying N for early growth of IR64 rice cultivars (2.34 and 2.13 %N). The ability of these isolates to fix atmospheric N2 was similar to N fertilizer (urea) in supplying N (2.2% N). Only Azotobacter T.B.PDST.2b was relatively significant to supply N (1.36% N) for Siam Unus rice cultivar early growth, even though this strain has less ability than N fertilizer (1.94% N) in supplying N. Azotobacter isolates grow in media containing no urea were higher IAA produced than in media containing Urea. Furthermore, yield of IR64, Margasari and Siam Unus inoculated with Azotobacter isolates were 3.87–4.93; 4.63-5.36 and 5.44-6.42 ton/ha respectively. In conclusion, the utilization of Azotobacter and Azorpirillum spp to substitute N fertilizer would be able to increase effeciency of N nutritions and to avoid environment pollutions risks from agrochemical N fertilizer other than to increase rice yields on tidal land of South Kalimantan.Keywords: Azotobacter, Azospirillum, rice cultivars, tidal lands and rice yields.

INDONESIAN FARMERS CAN CONTRIBUTE IN REDUCING GREENHOUSE GASES EMISSION FROM WETLAND RICE FIELD Iswandi Anas; Nia K. Megasari; Suprihati Suprihati; Hiroyuki OHTA
Jurnal Ilmu Tanah dan Lingkungan Vol 10 No 2 (2008): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Agricultural sector contribute significantly to the total GHG emission. There are some GHG mitigation options available but so far non of these mitigation options is being implemented by farmers. There is no real action have been taken by government to encourage farmers to implement the available mitigation option to reduce GHG emission from wetland rice field. We discuss some mitigation options and we suggest two additional GHG mitigation options namely crops rotation and System of Rice Intensification (SRI) that probably more attractive to farmer to implement them. However the impacts of these two proposed options as GHG mitigation options should be studied further.Key words: Greenhouse gases (GHG), mitigation option, farmer contribution, SRI, crop rotation

UJI EFEKTIVITAS BIO-ORGANIC FERTILIZER (PUPUK ORGANIK HAYATI) DALAM MENSUBSTITUSI KEBUTUHAN PUPUK ANORGANIK PADA TANAMAN SWEET SORGHUM [SORGHUM BICOLOR (L.) MOENCH] Ester LN Lumbantobing; Fahrizal Hazra; Iswandi Anas
Jurnal Ilmu Tanah dan Lingkungan Vol 10 No 2 (2008): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

The aim of research was to examine the effectiveness of bio-organic fertilizer to substitute inorganic fertilizer necessity on sweet sorghum [Sorghum bicolor (L.) Moench] vegetative growth. This research was carried out in the University Farm green house Cikabayan and in the Leuwikopo Experiment Field, Institut Pertanian Bogor. The green house experiment used completely randomized design with six treatments, namely: 1. No fertilizer (control), 2. 100%NPK, 3. 50%N + DOP, 4. 50%NPK + Fertismart, 5. 50%NPK + Ponti, dan 6. 50%NPK + Biost. The field experiment used completely randomizes block design with seven treatments, namely: 1. No fertilizer (control), 2. 50%NPK, 3. 100%NPK, 4. 50%N + DOP, 5. 50%NPK + Fertismart, 6. 50%NPK + Ponti, and 7. 50%NPK + Biost. Materials which was used were super sugar variety, NPK fertilizers, bio-organic fertilizer Fertismart, Ponti, Biost, and organic fertilizer Double Organic Phosphate (DOP).The results of greeen house research showed that bio-organic fertilizer could increase crop height, number of leaves, wet weight upper crop, dry weight upper crop, wet weight root, and dry weight root of sweet sorghum than control and same with treatment of 100%NPK. Treatment of 50%NPK + Biost could increase crop height (47.8%) than control and same with treatment of 100%NPK. Treatment of 50%NPK + Ponti increase dry weight root (59%) than control and same with treatment of 100%NPK.The results of field research showed that bio-organic fertilizer Fertismart could increase wet weight upper crop, crop height (42.8%) and number of leaves (39.5%) than control, and same with 100%NPK. It means that bio-organic fertilizer could substitute about 50% inorganic necessity on sweet sorghum.Keywords: bio-organic fertilizer, Azotobacter, Azospirillum, phosphate solubilizing microbes.

VIABILITAS INOKULAN DALAM BAHAN PEMBAWA GAMBUT, KOMPOS, ARANG BATOK DAN ZEOLIT YANG DISTERIL DENGAN IRADIASI SINAR GAMMA Co-60 DAN MESIN BERKAS ELEKTRON Sindy Marieta Putri; Iswandi Anas; Fahrizal Hazra; Ania Citraresmini
Jurnal Ilmu Tanah dan Lingkungan Vol 12 No 1 (2010): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Carrier is one of the important factor to determine the quality of biofertilizer. The inoculant carriers should contain no or less microbial contaminant. The purpose of this research was to investigate viability of Azospirillum, Azotobacter and Phosphate Solubilizing Fungi inoculants in carrier material that were sterilized by Gamma Irradiation Co-60 and Electron Beam Machine (EBM). Each inoculants was injected to the sterilized peat, compost, coconut shell charcoal and zeolite. Viability of inoculants in steriled carrier was evaluated at 0, 7, 14, 42 and 70 days after preparation. The stirage was done in incubator at 25ºC. The result of viability of Azospirillum, Azotobacter and Phosphate Solubilizing Fungi inoculants on sterilized carrier material by Gamma Irradiation Co-60, EBM and autoclave tended to decline during storage 70 days at room temperature (250C). Viability of Azospirillum inoculants in zeolite sterilized by Gamma Irradiation Co-60 and autoclave gave the highest numbres of viable cells. Storage of these inoculants at 25ºC for 70 days only reduce the number of viable cells by 11.1%. Viability of Phosphate Solubilizing Fungi inoculants in coconut shell charcoal or compost sterilized by Gamma Irradiation Co-60 went down by 99.8% after 70 days. The sterilization of carriers by using autoclave or Gamma Irradiation Co-60 were better than EBM sterilization. . Keywords: Carrier, Electron Beam Machine, Gamma Irradiation Co-60, sterilization, viability

PROFIL KELARUTAN LIMBAH MINYAK BERAT AKIBAT PENAMBAHAN BAHAN PENCAMPUR Charlena Charlena; Iswandi Anas; Zainal Alim Mas'ud; Ahmad Syahreza; Niken Dyah Wanodyanti
Jurnal Ilmu Tanah dan Lingkungan Vol 12 No 1 (2010): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Heavy Oil Waste (HOW) is on of the most important petroeum waste. In the recent years, many contamination of soil or water by HOW have been reported. Bioremediation is one of the alternative technology to clean the HOW contaminated soil since it is enviromentally friendly, effective, efficient and low cost. The contaminated soil is diluted in water to form bioslurry. However, the solubility of HOW in water is very low. To increase HOW solubility in water, addition of four mixing agents were tested namely: Sodium Tripolyphosphate (STPP), Carboxymethyl cellulose (CMC), quart sand and pumice stone. The main characteristics of HOW were solid TPH was 17.2%, water content was 1.96% and Cu and Hg content was 1.49 ppm and 3.33 ppb, respectively. The parameters determined were turbidity, solid TPH, liquid TPH pH and COD. The results showed that (1) the highest turbidity was in the STPP addition; (2) the lowest solid TPH was in the addition of pumice stone; (3) the highest liquid TPH was in the STPP addition;(4) the pH varied from 3.6 to 7.9; (5) the ghigest COD was in the addition of STPP. From these results it can be concluded that the best mixing agent to increase the solubility of HOW in soil slurry was STPP. Keywords: heavy oil waste (HOW), mixing agents, solubility of HOW

APLIKASI PUPUK ANORGANIK DAN ORGANIK HAYATI PADA BUDIDAYA PADI SRI (System of Rice Intensification) Muchlis Muhammad Bakrie; Iswandi Anas; Sugiyanta Sugiyanta; Komaruddin Idris
Jurnal Ilmu Tanah dan Lingkungan Vol 12 No 2 (2010): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Excessive use of inorganic fertilizers mainly NPK causes soil degradation, environment pollution, decreases production, and reduces soil biological activity. System of rice intensification is one method of rice cultivation. SRI main principles are seed transplanting at young age (7-11 days old), transplanting use single seedling methode, seedlings at wide plant spacing ≥ 25x25 cm, intermittent irrigation and field conditions are not flooded, and reduction of chemical fertilizers and application of organic fertilizer. This research was conducted at Situgede Village, West Bogor District, Bogor. The research design was split plot randomized complete block design with three blocks. Two rice cultivation system as the main plot and five combinations of fertilizer application as sub plot. In the main plot consisted of two rice cultivation which is conventional and SRI while the subplot consisted of five combinations of fertilizer application is no fertilition, 100% inorganic fertilizers (Urea = 250 kg ha-1, SP-36 = 75 kg ha-1 and KCl = 50 kg ha-1), 75% inorganic fertilizers (Urea = 187 kg ha-1, SP-36 = 56.8 kg ha-1 and KCl = 37.5 kg ha-1) + 200 kg of bio-organicfertilizer, 50% inorganic fertilizers (Urea = 125 kg ha-1, SP-36 = 37.5 kg ha-1 and KCl = 25 kg ha-1) + 200 kg of bio-organicfertilizer and 50% inorganic fertilizers (Urea = 125 kg ha-1, SP-36 = 37.5 kg ha-1 and KCl = 25 kg ha-1). The results showed that SRI method produced maximum number of tillers higher that of 25.8 tillers/hill or an increase of 64.33% compared with conventional methods. Wet and dry shoot weight of wet and dry weight of root is greater in successive SRI method of 13.3%, 19.1%, 1.40% and 41.8% compared with the conventional method. The number of productive tillers, grain number/panicle, 1000 grains weight, root wet weight and dry grain at SRI method was higher than those in conventional method respectively 58.6%, 37.0%, 2.50%, 25.1% and 32.6%. The uptake of N and P in the SRI method higher at 72.0% and 100% compared to conventional method. Application 50% inorganic fertilizer + 200 kg bio-organic fertilizer, producing more fresh weight biomass, plant dry weight, wet weight and dry weight, number of productive tillers, 1000 grain weight respectively 13.9%, 42.0%, 49.8%, 74.0%, 10.7% and 2.48% compared with the dosage recommendations dose or 100% inorganic fertilizer. N, P and K uptake at 50% inorganic fertilizer + 200 kg of bio-organic fertilizer treatment higher (55.6%, 66.7% and 46.2%) than the full recommendation dose of inorganic fertilizer. Bio-organic fertilizer can be used as substitute of NPK fertilizer.Keywords : Bio-organic fertilizer, inorganic fertilizer, System of Rice Intensification (SRI)

PRODUKSI MASAL INOKULUM AZOTOBACTER, AZOSPIRILLUM DAN BAKTERI PELARUT FOSFAT DENGAN MENGGUNAKAN MEDIA ALTERNATIF Richard Gunawan; Iswandi Anas; Fahrizal Hazra
Jurnal Ilmu Tanah dan Lingkungan Vol 12 No 2 (2010): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Azotobacter, Azospirillum and phosphate solubilizing bacteria are the most common microbial inoculants used as biofertilizer. To have good quality of biofertilizer, the high number of inoculant cells and suitable carriers as well as the method of carrier sterilization are among the most important factors determined the quality of biofertilizer. Related to the number of inoculant cells in carriers, the growing medium used to cultivate the microbial cells play very important role. For mass production of microbial cells, the medium should be able to support fast growth of microbial cells. The price of medium should be reasonably cheap and the materials used in medium should be available easily. The purpose of this study was to obtain a cheap growing medium that can support high number of microbial inoculant cells and the components of the medium should be easyly obtain and the price is not expensive. The study was conducted at the Department of Soil Science and Land Resources, Faculty of Agriculture, Bogor Agricultural University (IPB). The results showed that the medium IPB RI-1 was able to support the growth of 1010 cfu ml-1 Azotobacter, 108 cfu ml-1 Azospirillum and 109 cfu ml-1 Phosphate Solubilizing Bacteria. The number of bacterial cells in Nutrient Broth medium was only 108 cfu ml-1. This means that the IPB RI-1 medium was able to produce 100-fold population of Azotobacter compared to the growth of this bacterium in Nutrient Broth and Phosphate Solubilizing Bacteria was 10-fold higher than population in Nutrient Broth medium. The costs of the IPB RI-1 and IPB RI-2 were much cheaper compared to the cost of Nutrient Broth medium. The cost of medium IPB RI-1 only 3% (IDR 945) and IPB RI-2 about 2% (IDR 690) of the cost of Nutrient Broth medium (IDR 27,752) per liter medium in the year of 2010.Keywords : Alternative media, Azotobacter, Azospirillum, Nutrient Broth, Phosphate Solubilizing Bacteria

STUDI MIKROBIOLOGI DAN SIFAT KIMIA MIKROORGANISME LOKAL (MOL) YANG DIGUNAKAN PADA BUDIDAYA PADI METODE SRI (System of Rice Intensification) Arum Asriyanti Suhastyo; Iswandi Anas; Dwi Andreas Santosa; Yulin Lestari
Sainteks Vol 10, No 2 (2013): SAINTEKS
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v10i2.148

Penggunaan larutan mikroorganisme lokal (MOL) dalam metode SRI budidaya padi yang dikembangkan di Indonesia dimulai awal sejak persiapan bibit fase vegetatif, pembentukan malai dan pengisian bulir padi. MOL adalah cairan yang dapat dibuat dari bahan yang tersedia di sekitar kita seperti sisa sayuran, rebung, keong mas, buah maja, daun gamal, bonggol pisang, nasi, urine kelinci, dan lain-lain. Cairan umumnya diberikan 10, 20, 30, 40 dan 60 hari setelah tanam (HST ) atau sesuai kebutuhan. Penelitian ini menggunakan larutan MOL yang terbuat dari bonggol pisang, keong mas dan urin kelinci. Tujuan penelitian adalah untuk menentukan mikrobaa, identifikasi mikrobaa dan sifat kimia dalam MOL bonggol pisang, keong mas dan urin kelinci. Penelitian ini dilakukan di Laboratorium Bioteknologi Tanah IPB. Penelitian ini menggunakan rancangan acak lengkap dengan satu faktor (waktu) dan tiga kali pengulangan. Hasil penelitian menunjukkan MOL bonggol pisang memiliki rata-rata nilai pH terendah (4,2-4,5) dan nilai EC tertinggi (10,44-12,82 mikrodetik/cm) selama proses fermentasi. MOL keong emas memiliki rata-rata pH tertinggi (4,5-6,55) dan dan yang paling oksidatif - reduktif nilai Eh [ 269-(-381) mV ] selama proses fermentasi. MOL urin kelinci memiliki nilai rata-rata terendah dari EC (2,18-2,23 mikrodetik/cm) dan mengandung lebih banyak unsur K, Ca, Mg, Cu, Zn, Fe dan Mg dari kedua jenis MOL lainnya. Selanjutnya, Bacillus sp., Aeromonas sp. dan Aspergillus niger diidentifikasi dalam MOL dari bonggol pisang. MOL dari keong mas mengandung Staphylococcus sp. dan Aspergillus niger, sedangkan MOL urin kelinci memiliki Bacillus sp. , Rhizobium sp. , Pseudomonas sp. , Aspergillus niger dan Verticillium sp. Kata kunci : SRI, MOL, sifat kimia MOL, identifikasi mikrobaa

Soil Biology Contribution on Agricultural Land Suitability Evaluation of Wet Tropical Megabiodiversity Regions SUBOWO GITOSUWONDO; EDI SANTOSA; ISWANDI ANAS
Jurnal Sumberdaya Lahan Vol 4, No 2 (2010)
Publisher : Indonesian Center for Agriculture Land Resource Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jsdl.v4n2.2010.%p

Indonesia is in the region "wet tropical megabiodiversity" it would need to complete the system for agricultural land suitability evaluation in accordance with real conditions, thus increasing the value-added resources optimally and sustainable and accountable production investment. Soil organisms as a component of production support can act as an agent of energy and nutrient cycling in the soil, improving soil physical properties, and controlling pests and disease. For that soil, biological parameters that need to be considered in evaluating the suitability of land that already exist include: N-fixing or P-solubilizing bacteria groups that live symbiosis and free-living, the fungi solubilizing P and soil organic matter decomposition groups, BGA fixing and free-living N symbiotic groups, fauna groups are able to conserve soil organic matter and improve soil physical properties. Important steps that need to be done in the evaluation of land cover inventory of soil biological populations and its role on the growth of crops, and evaluate the suitability of the soil biological parameters of the functional value of commodity options.

Peranan rizobakteri dan fungi mikoriza arbuskular dalam proses fotosintesis dan produksi gula sorgum manis (Sorghum bicolor L. Moench) Role of rhizobacteria and arbuscular mycorrhizal fungi in increasing photosynthesis process and sugar production of sweet sorghum (Sorghum bicolor L. Moench) Bedah RUPAEDAH; Iswandi ANAS; Dwi Andreas SANTOSA; Wahono SUMARYONO; Sri Wilarso BUDI
E-Journal Menara Perkebunan Vol 83, No 1: Juni 2015
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

AbstractDual inoculation effects of rhizobacteria andAMF as well as the addition of chemical fertilizerson photosynthesis were studied by analyzingprocess of CO2 gas exchange, chlorophyll content,nutrient uptake, sugar content and growth of sweetsorghum. AMF inoculation as a single cultureincreased carbon assimilation, stomatal conductance,intercellular CO2 concentration, phosphoruscontent, chlorophyll content, sugar content, plantheight, shoot weight and mycorrhizal colonization.Interaction of AMF and rhizobacteria increasedsugar content of sweet sorghum stems andpotassium content of sweet sorghum leaves,whereas its interaction with chemical fertilizerssignificantly increased chlorophyll content, sugarcontent and mycorrhizal colonization of sorghumplant roots. Interaction of AMF, rhizobacteria andchemical fertilizers increased sugar content, rootweight and mycorrhizal colonization. The use ofAMF solely or its interaction with rhizobacteriaand chemical fertilizers had a great potential inimproving photosynthesis process of sweetsorghum. The process is associated with increasingcrop productivity, such as sugar content ofsorghum which is potential as a source ofrenewable energy.AbstrakPengaruh inokulasi ganda rizobakteri danFMA dengan penambahan pupuk kimia dipelajaridengan cara menganalisis proses pertukaran gas CO2, kandungan klorofil, kandungan hara dan gula,pertumbuhan dan produktivitas sorgum manis.Inokulasi FMA sebagai kultur tunggal dapatmeningkatkan asimilasi karbon, konduktansistomata, konsentrasi CO2 interselular, kandunganfosfor, kandungan klorofil, kandungan gula, tinggitanaman, berat batang dan derajat kolonisasimikoriza. Interaksi antara rizobakteri dan FMAdapat meningkatkan kandungan gula batang dankalium daun sorgum manis, sedangkan interaksinyadengan pupuk kimia dapat meningkatkan kandunganklorofil dan gula serta derajat kolonisasimikoriza pada perakaran sorgum manis. Sementaraitu, interaksi FMA, rizobakteri dan pupuk kimiadapat meningkatkan kandungan gula batang, beratakar dan derajat kolonisasi mikoriza pada perakaransorgum manis. Penggunaan FMA baik sendirimaupun interaksinya dengan rizobakteri dan pupukkimia memiliki potensi besar dalam meningkatkanproses fotosintesis sorgum manis. Proses tersebutberkaitan dengan peningkatan produktivitas sorgummanis dalam hal ini kandungan gula sorgum manisyang berpotensi sebagai sumber energi terbarukan.

Co-Authors Abdul Hasyim Sodiq Abha Mishra Ahmad Syahreza Amir Kassam Amod K. Thakur Ania Citraresmin Ania Citraresmini Arum Asriyanti Suhastyo Atang Sutandi Bariot Hafif Bedah Rupaedah Charlena Dedi Nursyamsi DWI ANDREAS SANTOSA Edi Santosa Ester LN Lumbantobing Fahrizal Hazra Fakhrur Razie Grahan Sugeng Aprilian Heni Hariyani Hiroyuki OHTA Indriyati, Lilik Tri J Heinzemann Joeli Barison Komaruddin Idris Luh Putu Ratna Sundari Lukman Gunarto Muchlis Muhammad Bakrie Nia K. Megasari Niken Dyah Wanodyanti Noegroho Hadi Norman Uphoff O.P Rupela Richard Gunawan Rosinta Sitepu Sindy Marieta Putri Sri Wilarso Budi SUBOWO GITOSUWONDO Sukmadewi, Desak Ketut Tristiana Suprihati Suwarno Suwarno Suyamto Suyamto T Yulliawati T. M. Thiyagarajan Triyani Dewi Wahono Sumaryono Windi Windi YULIN LESTARI Yuwan Pratama Baki Zainal Alim Mas'ud

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