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AKTIVITAS ENZIM SELULASE MIKROBA YANG DIISOLASI DARI JERAMI PADI DI PERSAWAHAN PASANG SURUT DI KALIMANTAN SELATAN Fakhrur Razie; Iswandi Anas; Atang Sutandi; Lukman Gunarto; Sugiyanta Sugiyanta
Jurnal Ilmu Tanah dan Lingkungan Vol 13 No 2 (2011): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.775 KB) | DOI: 10.29244/jitl.13.2.43-48

Abstract

Enzim selulase terdiri dari tiga enzim ekstraselular yang bekerja secara sinergis dalam mendegredasi selulosa, yakni endoglukanase, eksoglukanase dan β-glukosidase. Tiga enzim tersebut berperan dalam mendegradasi selulosa menjadi gula sederhana. Penelitian ini bertujuan untuk mengisolasi dan menyeleksi mikroba (bakteri dan fungi) berdasarkan aktivitas enzim selulase dari mikroba tanah yang diisolasi dari persawahan pasang surut Kalimantan Selatan. Kemampuan mengekskresikan enzim endoglukanase dinilai berdasarkan nilai indeks selulolitik pada media CMC dan kemampuan mengekskresikan enzim eksoglukanase dan β-glukosidase diukur dari aktivitas kedua enzim tersebut menggunakan metode Mandel yang dimodifikasi. Hasil yang diperoleh menunjukkan bahwa kisaran nilai indeks selulolitik dari mikroba selulolitik di persawahan pasang surut tipe A sebesar 2.29-3.72, di lahan tipe B sebesar 2.66-5.41, dan di lahan tipe C sebesar 1.84-3.34. Aktivitas eksoglukanase dari mikroba selulolitik di persawahan pasang surut tipe A sebesar 0.27-1.65 nkat mL-1, lahan tipe B sebesar 0.37-1.85 nkat mL-1, dan lahan tipe C sebesar 0.31-1.85 nkat mL-1. Mikroba selulolitik dari persawahan pasang surut Kalimantan Selatan memiliki aktivitas β-glukosidase sebesar 0.05-1.52 nkat mL-1. Isolat- isolat mikroba selulolitik yang memiliki aktivitas selulase tertinggi adalah isolat bakteri selulolitik J11, J42, R23, BK12, C52, TB41, B82 dan SN123, dan isolat fungi selulolitik ST33, ST22, TB31, B52, GA22, TD11, PI52 dan P31.
THE SYSTEM OF RICE INTENSIFICATION (SRI) AS A BENEFICIAL HUMAN INTERVENTION INTO ROOT AND SOIL INTERACTION Iswandi Anas; Joeli Barison; Amir Kassam; Abha Mishra; O.P Rupela; Amod K. Thakur; T. M. Thiyagarajan; Norman Uphoff
Jurnal Ilmu Tanah dan Lingkungan Vol 13 No 2 (2011): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (611.752 KB) | DOI: 10.29244/jitl.13.2.72-88

Abstract

The System of Rice Intensification (SRI) was developed in Madagascar in the ealier 1980 by Fr. Henri de Laulanié. Basic principles of SRI are: (1) the transplanting of young seedlings, preferably only 8-12 days old, this conserves the growth potential that rice plants have if they are transplanted before the start of the fourth phyllochron; (2) The young seedlings are transplanted quickly and quite carefully, taking care to minimize any trauma to the roots, also singly and with wide spacing, in a square pattern usually 25 cm x 25 cm, or even farther apart if the soil is fertile; (3) Under SRI management, paddy fields are not kept continuously flooded, instead, mostly aerobic soil conditions are maintained throughout the vegetative growth period, either by adding small amounts of water regularly, or by alternate wetting and drying (AWD); (4) a simple mechanical, soil-aerating weeder is used to control weed growth; (5) Although these methods when used with chemical fertilizer will enhance crop yield, the best yields and greatest cost-saving for farmers are attained with application of organic fertilizer or other organic matter, when available. When SRI practices are used together and as recommended, the following results are common: (1) Grain yields are usually increased by 50-100%, or sometimes more, while water applications are reduced by 30-50% since there is no continuous flooding, straw yields usually also increase, which is an additional benefit to many farmers; (2) The need to use agrochemicals for crop protection is reduced because SRI plants are naturally more resistant to pest and disease damage; (3) With reduced costs of production, including often reduced labor requirements, farmers’ net income is greatly increased with the higher yields; (4) SRI plants are better suited to withstand the effects of climate change, having greater resistance as a rule to most biotic and abiotic stresses; (5) SRI paddy usually gives higher milling out-turn, about 15%, because when milled there is less chaff (fewer unfilled grains) and less breaking of grains. These qualities are probably attributable to the effects of better root systems which can more effectively take up micronutrients from lower soil horizons. Currently, SRI practices has been introduced in many countries with modifications and adaptation to local conditions.
POLISAKARIDA DAN STABILITAS AGREGAT TANAH MASAM YANG DIPERLAKUKAN DENGAN BRACHIARIA, MIKORIZA DAN KOMPOS JERAMI DIPERKAYA KALIUM Bariot Hafif; Supiandi Sabiham; Iswandi Anas; Atang Sutandi; Suyamto Suyamto
Jurnal Ilmu Tanah dan Lingkungan Vol 13 No 1 (2011): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.041 KB) | DOI: 10.29244/jitl.13.1.1-7

Abstract

Stabilitas agregat menentukan kualitas tanah dan polisakarida adalah agen agregasi utama partikel tanah. Penelitian bertujuan mempelajari stabilitas agregat dan polisakarida sebagai agen agregasi partikel tanah masam yang diperlakukan dengan Brachiaria decumbens (BD), mikoriza dan kompos jerami diperkaya kalium di Kebun Percobaan Tegineneng BPTP Lampung. Penelitian ini menggunakan rancangan acak lengkap faktorial 3 faktor. Faktor 1, rumput Brachiaria decumbens, yaitu tanpa B. decumbens (B0) dan dengan baris B. decumbens (B1); faktor 2, mikoriza yaitu tanpa mikoriza (M0) dan dengan inokulasi mikoriza (M1); dan faktor 3, kompos jerami diperkaya kalium yaitu kompos 2 ton ha-1 masing-masing diperkaya KCl masing-masing 0 kg ha-1 (K0), 50 kg ha-1 (K50), 100 kg ha-1 (K100) dan 200 kg ha-1 (K200). Hasil penelitian menunjukkan perlakuan BD dan interaksi BD dan mikoriza mendorong fragmentasi agregat makro menjadi agregat meso dan mikro, namun stabilitas agregat dibawah pengaruh perlakuan tersebut lebih baik dibanding stabilitas agregat tanah kontrol. Inokulasi mikoriza memperbaiki stabilitas agregat makro 1-2 mm. Pengayaan kalium pada kompos jerami secara rata-rata tidak berpengaruh terhadap stabilitas agregat tetapi dalam interaksi dengan B. decumbens, pengayaan kompos jerami dengan 100 dan 200 kg KCl ha-1 berpengaruh cukup baik terhadap stabilitas agregat makro 2-5 mm. Polisakarida total di dalam agregat tanah pada perlakuan interaksi B. decumbens dan mikoriza nyata meningkat, demikian juga polisakarida bukan selulosa cenderung lebih baik. Perlakuan B. decumbens meningkatkan kadar polisakarida total di dalam agregat meso (0.25-1 mm) dan mikro (0.05-0.25 mm), sedangkan mikoriza meningkatkan polisakarida total dan polisakarida bukan selulosa di dalam agregat makro (> 1 mm).
EFFECT OF AZOTOBACTER AND AZOSPIRILLUM ON GROWTH AND YIELD OF RICE GROWN ON TIDAL SWAMP RICE FIELD IN SOUTH KALIMANTAN Fakhrur Razie; Iswandi Anas
Jurnal Ilmu Tanah dan Lingkungan Vol 10 No 2 (2008): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.55 KB) | DOI: 10.29244/jitl.10.2.41-45

Abstract

Tidal swamp land is a potential area for rice cultivation. However, tidal swamp is fragile ecosystems, so that when this area is used for rice cultivation, it has to be done carefully. To reduce a risk of environmental pollution in the tidal swamp area due to rice cultivation, the area should be managed properly and wisely especially when using agrochemicals such as fertilizers and pesticides. In relation to this, the use of bio-fertilizer such as Azotobacter or/and Azospirillum, an atmospheric nitrogen fixing bacterium, might be an important thing for this area. The objectives of this study were to evaluate the number of NFB (i.e. Azotobacter and Azospirillum spp.) found in tidalswamp rice fields of South Kalimantan, and their ability in fixing atmospheric nitrogen and supplying this fixed nitrogen to rice, and in increasing rice yields. There were three research stages performed in this study. The first, collecting, isolating and purifying the NFB on the selective media of nitrogen free media. The second, a hydroponic experiment in the greenhouse using Yoshida nutrition solution (Yoshida et al., 1976 in IRRI, 2003) as culture media and selected Azotobacter and Azospirillum. The third experiment was to study the effect of selected Azotobacter isolates to increase growth and yield of IR64 and Margasari rice cultivars, and two Azotobacter isolates were combined with six inoculation methods for Siam Unus rice cultivar as treatments. The result showed that the number of NFB strains found in rhizospere rice cultivars were varied widely. Then, Azotobacter and Azospirillum spp selected from rice fields in the tidal land of South Kalimantan when associated with IR64 and Siam Unus rice cultivars have ability in fixing atmospheric N2 and in supplying N on the initial rice growth. Azotobacter T.B.PDST.2b and T.HM.BPMT.2b were significantly supplying N for early growth of IR64 rice cultivars (2.34 and 2.13 %N). The ability of these isolates to fix atmospheric N2 was similar to N fertilizer (urea) in supplying N (2.2% N). Only Azotobacter T.B.PDST.2b was relatively significant to supply N (1.36% N) for Siam Unus rice cultivar early growth, even though this strain has less ability than N fertilizer (1.94% N) in supplying N. Azotobacter isolates grow in media containing no urea were higher IAA produced than in media containing Urea. Furthermore, yield of IR64, Margasari and Siam Unus inoculated with Azotobacter isolates were 3.87–4.93; 4.63-5.36 and 5.44-6.42 ton/ha respectively. In conclusion, the utilization of Azotobacter and Azorpirillum spp to substitute N fertilizer would be able to increase effeciency of N nutritions and to avoid environment pollutions risks from agrochemical N fertilizer other than to increase rice yields on tidal land of South Kalimantan.Keywords: Azotobacter, Azospirillum, rice cultivars, tidal lands and rice yields.
INDONESIAN FARMERS CAN CONTRIBUTE IN REDUCING GREENHOUSE GASES EMISSION FROM WETLAND RICE FIELD Iswandi Anas; Nia K. Megasari; Suprihati Suprihati; Hiroyuki OHTA
Jurnal Ilmu Tanah dan Lingkungan Vol 10 No 2 (2008): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.274 KB) | DOI: 10.29244/jitl.10.2.54-59

Abstract

Agricultural sector contribute significantly to the total GHG emission. There are some GHG mitigation options available but so far non of these mitigation options is being implemented by farmers. There is no real action have been taken by government to encourage farmers to implement the available mitigation option to reduce GHG emission from wetland rice field. We discuss some mitigation options and we suggest two additional GHG mitigation options namely crops rotation and System of Rice Intensification (SRI) that probably more attractive to farmer to implement them. However the impacts of these two proposed options as GHG mitigation options should be studied further.Key words: Greenhouse gases (GHG), mitigation option, farmer contribution, SRI, crop rotation
UJI EFEKTIVITAS BIO-ORGANIC FERTILIZER (PUPUK ORGANIK HAYATI) DALAM MENSUBSTITUSI KEBUTUHAN PUPUK ANORGANIK PADA TANAMAN SWEET SORGHUM [SORGHUM BICOLOR (L.) MOENCH] Ester LN Lumbantobing; Fahrizal Hazra; Iswandi Anas
Jurnal Ilmu Tanah dan Lingkungan Vol 10 No 2 (2008): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.815 KB) | DOI: 10.29244/jitl.10.2.72-76

Abstract

The aim of research was to examine the effectiveness of bio-organic fertilizer to substitute inorganic fertilizer necessity on sweet sorghum [Sorghum bicolor (L.) Moench] vegetative growth. This research was carried out in the University Farm green house Cikabayan and in the Leuwikopo Experiment Field, Institut Pertanian Bogor. The green house experiment used completely randomized design with six treatments, namely: 1. No fertilizer (control), 2. 100%NPK, 3. 50%N + DOP, 4. 50%NPK + Fertismart, 5. 50%NPK + Ponti, dan 6. 50%NPK + Biost. The field experiment used completely randomizes block design with seven treatments, namely: 1. No fertilizer (control), 2. 50%NPK, 3. 100%NPK, 4. 50%N + DOP, 5. 50%NPK + Fertismart, 6. 50%NPK + Ponti, and 7. 50%NPK + Biost. Materials which was used were super sugar variety, NPK fertilizers, bio-organic fertilizer Fertismart, Ponti, Biost, and organic fertilizer Double Organic Phosphate (DOP).The results of greeen house research showed that bio-organic fertilizer could increase crop height, number of leaves, wet weight upper crop, dry weight upper crop, wet weight root, and dry weight root of sweet sorghum than control and same with treatment of 100%NPK. Treatment of 50%NPK + Biost could increase crop height (47.8%) than control and same with treatment of 100%NPK. Treatment of 50%NPK + Ponti increase dry weight root (59%) than control and same with treatment of 100%NPK.The results of field research showed that bio-organic fertilizer Fertismart could increase wet weight upper crop, crop height (42.8%) and number of leaves (39.5%) than control, and same with 100%NPK. It means that bio-organic fertilizer could substitute about 50% inorganic necessity on sweet sorghum.Keywords: bio-organic fertilizer, Azotobacter, Azospirillum, phosphate solubilizing microbes.
VIABILITAS INOKULAN DALAM BAHAN PEMBAWA GAMBUT, KOMPOS, ARANG BATOK DAN ZEOLIT YANG DISTERIL DENGAN IRADIASI SINAR GAMMA Co-60 DAN MESIN BERKAS ELEKTRON Sindy Marieta Putri; Iswandi Anas; Fahrizal Hazra; Ania Citraresmini
Jurnal Ilmu Tanah dan Lingkungan Vol 12 No 1 (2010): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (632.597 KB) | DOI: 10.29244/jitl.12.1.23-30

Abstract

Carrier is one of the important factor to determine the quality of biofertilizer. The inoculant carriers should contain no or less microbial contaminant. The purpose of this research was to investigate viability of Azospirillum, Azotobacter and Phosphate Solubilizing Fungi inoculants in carrier material that were sterilized by Gamma Irradiation Co-60 and Electron Beam Machine (EBM). Each inoculants was injected to the sterilized peat, compost, coconut shell charcoal and zeolite. Viability of inoculants in steriled carrier was evaluated at 0, 7, 14, 42 and 70 days after preparation. The stirage was done in incubator at 25ºC. The result of viability of Azospirillum, Azotobacter and Phosphate Solubilizing Fungi inoculants on sterilized carrier material by Gamma Irradiation Co-60, EBM and autoclave tended to decline during storage 70 days at room temperature (250C). Viability of Azospirillum inoculants in zeolite sterilized by Gamma Irradiation Co-60 and autoclave gave the highest numbres of viable cells. Storage of these inoculants at 25ºC for 70 days only reduce the number of viable cells by 11.1%. Viability of Phosphate Solubilizing Fungi inoculants in coconut shell charcoal or compost sterilized by Gamma Irradiation Co-60 went down by 99.8% after 70 days. The sterilization of carriers by using autoclave or Gamma Irradiation Co-60 were better than EBM sterilization. . Keywords: Carrier, Electron Beam Machine, Gamma Irradiation Co-60, sterilization, viability
PROFIL KELARUTAN LIMBAH MINYAK BERAT AKIBAT PENAMBAHAN BAHAN PENCAMPUR Charlena Charlena; Iswandi Anas; Zainal Alim Mas'ud; Ahmad Syahreza; Niken Dyah Wanodyanti
Jurnal Ilmu Tanah dan Lingkungan Vol 12 No 1 (2010): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.946 KB) | DOI: 10.29244/jitl.12.1.31-35

Abstract

Heavy Oil Waste (HOW) is on of the most important petroeum waste. In the recent years, many contamination of soil or water by HOW have been reported. Bioremediation is one of the alternative technology to clean the HOW contaminated soil since it is enviromentally friendly, effective, efficient and low cost. The contaminated soil is diluted in water to form bioslurry. However, the solubility of HOW in water is very low. To increase HOW solubility in water, addition of four mixing agents were tested namely: Sodium Tripolyphosphate (STPP), Carboxymethyl cellulose (CMC), quart sand and pumice stone. The main characteristics of HOW were solid TPH was 17.2%, water content was 1.96% and Cu and Hg content was 1.49 ppm and 3.33 ppb, respectively. The parameters determined were turbidity, solid TPH, liquid TPH pH and COD. The results showed that (1) the highest turbidity was in the STPP addition; (2) the lowest solid TPH was in the addition of pumice stone; (3) the highest liquid TPH was in the STPP addition;(4) the pH varied from 3.6 to 7.9; (5) the ghigest COD was in the addition of STPP. From these results it can be concluded that the best mixing agent to increase the solubility of HOW in soil slurry was STPP. Keywords: heavy oil waste (HOW), mixing agents, solubility of HOW
APLIKASI PUPUK ANORGANIK DAN ORGANIK HAYATI PADA BUDIDAYA PADI SRI (System of Rice Intensification) Muchlis Muhammad Bakrie; Iswandi Anas; Sugiyanta Sugiyanta; Komaruddin Idris
Jurnal Ilmu Tanah dan Lingkungan Vol 12 No 2 (2010): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.442 KB) | DOI: 10.29244/jitl.12.2.25-32

Abstract

Excessive use of inorganic fertilizers mainly NPK causes soil degradation, environment pollution, decreases production, and reduces soil biological activity. System of rice intensification is one method of rice cultivation. SRI main principles are seed transplanting at young age (7-11 days old), transplanting use single seedling methode, seedlings at wide plant spacing ≥ 25x25 cm, intermittent irrigation and field conditions are not flooded, and reduction of chemical fertilizers and application of organic fertilizer. This research was conducted at Situgede Village, West Bogor District, Bogor. The research design was split plot randomized complete block design with three blocks. Two rice cultivation system as the main plot and five combinations of fertilizer application as sub plot. In the main plot consisted of two rice cultivation which is conventional and SRI while the subplot consisted of five combinations of fertilizer application is no fertilition, 100% inorganic fertilizers (Urea = 250 kg ha-1, SP-36 = 75 kg ha-1 and KCl = 50 kg ha-1), 75% inorganic fertilizers (Urea = 187 kg ha-1, SP-36 = 56.8 kg ha-1 and KCl = 37.5 kg ha-1) + 200 kg of bio-organicfertilizer, 50% inorganic fertilizers (Urea = 125 kg ha-1, SP-36 = 37.5 kg ha-1 and KCl = 25 kg ha-1) + 200 kg of bio-organicfertilizer and 50% inorganic fertilizers (Urea = 125 kg ha-1, SP-36 = 37.5 kg ha-1 and KCl = 25 kg ha-1). The results showed that SRI method produced maximum number of tillers higher that of 25.8 tillers/hill or an increase of 64.33% compared with conventional methods. Wet and dry shoot weight of wet and dry weight of root is greater in successive SRI method of 13.3%, 19.1%, 1.40% and 41.8% compared with the conventional method. The number of productive tillers, grain number/panicle, 1000 grains weight, root wet weight and dry grain at SRI method was higher than those in conventional method respectively 58.6%, 37.0%, 2.50%, 25.1% and 32.6%. The uptake of N and P in the SRI method higher at 72.0% and 100% compared to conventional method. Application 50% inorganic fertilizer + 200 kg bio-organic fertilizer, producing more fresh weight biomass, plant dry weight, wet weight and dry weight, number of productive tillers, 1000 grain weight respectively 13.9%, 42.0%, 49.8%, 74.0%, 10.7% and 2.48% compared with the dosage recommendations dose or 100% inorganic fertilizer. N, P and K uptake at 50% inorganic fertilizer + 200 kg of bio-organic fertilizer treatment higher (55.6%, 66.7% and 46.2%) than the full recommendation dose of inorganic fertilizer. Bio-organic fertilizer can be used as substitute of NPK fertilizer.Keywords : Bio-organic fertilizer, inorganic fertilizer, System of Rice Intensification (SRI)
PRODUKSI MASAL INOKULUM AZOTOBACTER, AZOSPIRILLUM DAN BAKTERI PELARUT FOSFAT DENGAN MENGGUNAKAN MEDIA ALTERNATIF Richard Gunawan; Iswandi Anas; Fahrizal Hazra
Jurnal Ilmu Tanah dan Lingkungan Vol 12 No 2 (2010): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (807.208 KB) | DOI: 10.29244/jitl.12.2.33-39

Abstract

Azotobacter, Azospirillum and phosphate solubilizing bacteria are the most common microbial inoculants used as biofertilizer. To have good quality of biofertilizer, the high number of inoculant cells and suitable carriers as well as the method of carrier sterilization are among the most important factors determined the quality of biofertilizer. Related to the number of inoculant cells in carriers, the growing medium used to cultivate the microbial cells play very important role. For mass production of microbial cells, the medium should be able to support fast growth of microbial cells. The price of medium should be reasonably cheap and the materials used in medium should be available easily. The purpose of this study was to obtain a cheap growing medium that can support high number of microbial inoculant cells and the components of the medium should be easyly obtain and the price is not expensive. The study was conducted at the Department of Soil Science and Land Resources, Faculty of Agriculture, Bogor Agricultural University (IPB). The results showed that the medium IPB RI-1 was able to support the growth of 1010 cfu ml-1 Azotobacter, 108 cfu ml-1 Azospirillum and 109 cfu ml-1 Phosphate Solubilizing Bacteria. The number of bacterial cells in Nutrient Broth medium was only 108 cfu ml-1. This means that the IPB RI-1 medium was able to produce 100-fold population of Azotobacter compared to the growth of this bacterium in Nutrient Broth and Phosphate Solubilizing Bacteria was 10-fold higher than population in Nutrient Broth medium. The costs of the IPB RI-1 and IPB RI-2 were much cheaper compared to the cost of Nutrient Broth medium. The cost of medium IPB RI-1 only 3% (IDR 945) and IPB RI-2 about 2% (IDR 690) of the cost of Nutrient Broth medium (IDR 27,752) per liter medium in the year of 2010.Keywords : Alternative media, Azotobacter, Azospirillum, Nutrient Broth, Phosphate Solubilizing Bacteria