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Peranan rizobakteri dan fungi mikoriza arbuskular dalam proses fotosintesis dan produksi gula sorgum manis (Sorghum bicolor L. Moench) Role of rhizobacteria and arbuscular mycorrhizal fungi in increasing photosynthesis process and sugar production of sweet sorghum (Sorghum bicolor L. Moench) Bedah RUPAEDAH; Iswandi ANAS; Dwi Andreas SANTOSA; Wahono SUMARYONO; Sri Wilarso BUDI
Menara Perkebunan Vol. 83 No. 1: 83 (1), 2015
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v83i1.13

Abstract

AbstractDual inoculation effects of rhizobacteria andAMF as well as the addition of chemical fertilizerson photosynthesis were studied by analyzingprocess of CO2 gas exchange, chlorophyll content,nutrient uptake, sugar content and growth of sweetsorghum. AMF inoculation as a single cultureincreased carbon assimilation, stomatal conductance,intercellular CO2 concentration, phosphoruscontent, chlorophyll content, sugar content, plantheight, shoot weight and mycorrhizal colonization.Interaction of AMF and rhizobacteria increasedsugar content of sweet sorghum stems andpotassium content of sweet sorghum leaves,whereas its interaction with chemical fertilizerssignificantly increased chlorophyll content, sugarcontent and mycorrhizal colonization of sorghumplant roots. Interaction of AMF, rhizobacteria andchemical fertilizers increased sugar content, rootweight and mycorrhizal colonization. The use ofAMF solely or its interaction with rhizobacteriaand chemical fertilizers had a great potential inimproving photosynthesis process of sweetsorghum. The process is associated with increasingcrop productivity, such as sugar content ofsorghum which is potential as a source ofrenewable energy.AbstrakPengaruh inokulasi ganda rizobakteri danFMA dengan penambahan pupuk kimia dipelajaridengan cara menganalisis proses pertukaran gas CO2, kandungan klorofil, kandungan hara dan gula,pertumbuhan dan produktivitas sorgum manis.Inokulasi FMA sebagai kultur tunggal dapatmeningkatkan asimilasi karbon, konduktansistomata, konsentrasi CO2 interselular, kandunganfosfor, kandungan klorofil, kandungan gula, tinggitanaman, berat batang dan derajat kolonisasimikoriza. Interaksi antara rizobakteri dan FMAdapat meningkatkan kandungan gula batang dankalium daun sorgum manis, sedangkan interaksinyadengan pupuk kimia dapat meningkatkan kandunganklorofil dan gula serta derajat kolonisasimikoriza pada perakaran sorgum manis. Sementaraitu, interaksi FMA, rizobakteri dan pupuk kimiadapat meningkatkan kandungan gula batang, beratakar dan derajat kolonisasi mikoriza pada perakaransorgum manis. Penggunaan FMA baik sendirimaupun interaksinya dengan rizobakteri dan pupukkimia memiliki potensi besar dalam meningkatkanproses fotosintesis sorgum manis. Proses tersebutberkaitan dengan peningkatan produktivitas sorgummanis dalam hal ini kandungan gula sorgum manisyang berpotensi sebagai sumber energi terbarukan.
Media Campuran Tanah-Pasir dan Pupuk Anorganik untuk Memproduksi Inokulan Cendawan Mikoriza Arbuskula (CMA) Iswandi Anas; J. L. O. Tampubolon
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 32 No. 1 (2004): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (592.639 KB) | DOI: 10.24831/jai.v32i1.1433

Abstract

AMF fungi are obligate biotroph, they can not be multiplied on artificial growth medium. The growth of AMF fungi depends on photosynthates of the host plant. The AMF inoculant production so far is done on special growth media such as vermicullite or zeolite and using a certain composition of fertilizer such as Hyponex (N: P: K = 25: 5:20). The aims of this experiment were (1) to study the possibility of using soil-sand mixture for growth medium in AMF inoculant production, and (2) to study the possibility of using Urea, SP-36 and KCI as fertilizers in AMF inoculant production. The results of the experiment showed that soil-sand mixture was a better growth medium for AMF inoculant production than zeolite. The number of AMF fungi spore, the root infection rate and the root biomass of the host plant (shorgum) were higher on soil-sand mixture growth medium compared to zeolite. Urea, SP-36 and KCI can be used as subtitute to Hyponex fertilizer in AMF inoculant production. Key words: CMA, Produksi inokulum, Entrophospora colombiana. Glomus manihotis
Tanggap Tanaman Kedelai terhadap Inokulasi Rhizobium dan Asam Indol Asetat (IAA) pada Ultisol Darmaga Rina D. Ningsih; Iswandi Anas
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 32 No. 2 (2004): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1699.641 KB) | DOI: 10.24831/jai.v32i2.1453

Abstract

Some of rhizobacteria have been known to stimulate the growth of some crops through their fitohormon (IAA = indole acetic acid). Those rhizobacteria can stimulate the development of epidermis cells formation at root hair  site and increase the infection sites to increase the nodulation and N2 fixation. The aims of this study were to study the effect of Rhizobium strains inoculation and indole acetic acid (IAA) application on crop growth, root nodulation. and N, P uptake of soybean on the Ultisols. The greenhouse experiment used Completely Randomize Design (CRD) with four replications. The treatments were: I) Without inoculation (blank), 2) 100 ppm N application, 3) 0.4 ppm IAA application, 4) Inoculation of Rhizobium 1004 (106), 5) Inoculation of Rhizobium 1004 (I05) + IAA, 6) Inoculation of  Rhizobium RD-20 (104), 7) Inoculation of Rhizobium RD-20 (106), 8) Inoculation of Rhizobium SNI-2 (106). 9) Inoculation of Rhizobium SNI-2 106 + IAA. Result of the experiment indicated that inoculation of Rhizobium and IAA application increased crop growth, nodulation, and nutrient uptake of soybean. Inoculation of Rhizobium I004(106). RD-20(104)R, D-20(I06),S NI-2(I06), and IAA 0.4 ppm increased dry weight of crop by 33.5%,37.8,17.3%,35.1%,and 3.8% respectively compared to blank. Application of IAA at Rhizobium inoculation treatment of SNI-2(106) and 1004(106i)n creased dry nodule weight on soybean 40.9%, and 55.7 % respectively compared to without IAA application.